Spermatozoa

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  Subject Areas on Research

  • A comparative analysis of embryo implantation potential in patients with severe teratozoospermia undergoing in-vitro fertilization with a high insemination concentration or intracytoplasmic sperm injection. 
  • A comparison of the relative efficiency of ICSI and extended culture with epididymal sperm versus testicular sperm in patients with obstructive azoospermia. 
  • A microliter oxygen electrode system for sperm suspensions. 
  • A paternal environmental legacy: evidence for epigenetic inheritance through the male germ line. 
  • A step toward solving some of the problems of cryopreservation. 
  • A study of the effect of perchloroethylene exposure on semen quality in dry cleaning workers. 
  • A technique for serial spermatozoa collection in mice. 
  • Adolescent cancer patients: sperm storage, consent and emotion. 
  • Alteration of cervical mucus by vanguard human spermatozoa. 
  • Alteration of human sperm kinematics in cervical mucus due to nonoxynol-9. 
  • An imprinted PEG1/MEST antisense expressed predominantly in human testis and in mature spermatozoa. 
  • Antiretroviral therapy effects on genetic and morphologic end points in lymphocytes and sperm of men with human immunodeficiency virus infection. 
  • Apoptosis in Xenopus egg extracts. 
  • Assessment of a new spermicidal agent against ejaculated dog and human spermatozoa in vitro. 
  • Axokinin phosphorylation by cAMP-dependent protein kinase is sufficient for activation of sperm flagellar motility. 
  • Beat frequency is bimodally distributed in spermatozoa from T/t12 mice. 
  • Biological basis of in vitro tests of sperm function. 
  • Ca2+ regulation of sperm axonemal motility. 
  • Cannabinoid exposure and altered DNA methylation in rat and human sperm. 
  • Cannabis use is associated with potentially heritable widespread changes in autism candidate gene DLGAP2 DNA methylation in sperm. 
  • Characterization and application of an avian in vitro spermatozoa-egg interaction assay using the inner perivitelline layer from laid chicken eggs. 
  • Characterization of moesin in the sea urchin Lytechinus variegatus: redistribution to the plasma membrane following fertilization is inhibited by cytochalasin B. 
  • Chemically induced mutations observed as mosaics in Drosophila melanogaster. 
  • Comparison of dose-response relationships for ethyl methanesulfonate and 1-ethyl-1-nitrosourea in Drosophila melanogaster spermatozoa. 
  • Comparison of intracervical, intrauterine, and intratubal techniques for donor insemination. 
  • Computer-aided sperm analysis (CASA): image digitization and processing. 
  • Computer-aided sperm analysis: technology at a crossroads. 
  • Conservation and regeneration of transgenic lines of swine by semen cryopreservation and artificial insemination. 
  • Cortisone acetate versus clomiphene citrate in per-germinal idiopathic oligospermia. 
  • Cyclic adenosine 3',5' monophosphate, calcium and protein phosphorylation in flagellar motility. 
  • Defending male fertility. 
  • Delayed fertilization during in vitro fertilization and embryo transfer cycles: analysis of causes and impact on overall results. 
  • Developmental changes in pituitary-gonadal function in free-ranging lions (Panthera leo leo) of the Serengeti Plains and Ngorongoro Crater. 
  • Differences in the movement of morphologically normal and abnormal human seminal spermatozoa. 
  • Differential extraction and enrichment of human sperm surface proteins in a proteome: identification of immunocontraceptive candidates. 
  • Diverse humoral and cell-mediated effects of antisperm antibodies on reproduction. 
  • Dosage-response relationships for methyl methanesulfonate in Drosophila melanogaster spermatozoa: DNA methylation per nucleotide vs. sex-linked recessive lethal frequency. 
  • Dosimetry of the chemical mutagen ethyl methanesulfonate in spermatozoan DNA from Drosophila melanogaster. 
  • Early indicators of male reproductive toxicity. 
  • Effect of mutagen-induced cell lethality on the dose response of germline mutations. 
  • Effect of vaginal lubricants on natural fertility. 
  • Effects of psychological stress on human semen quality. 
  • Embryonic development of human transplantation antigens. 
  • Endocrine dysfunction and clomiphene citrate therapy. 
  • Epigenetic alterations in cytochrome P450 oxidoreductase (Por) in sperm of rats exposed to tetrahydrocannabinol (THC). 
  • Ethical issues in gamete preservation for children undergoing treatment for cancer. 
  • Ethics of postmortem sperm retrieval: ethics of sperm retrieval after death or persistent vegetative state. 
  • Evidence for involvement of metalloendoproteases in a step in sea urchin gamete fusion. 
  • Evidence for the involvement of metalloendoproteases in the acrosome reaction in sea urchin sperm. 
  • Experience with subzonal insemination (SUZI) and intracytoplasmic sperm injection (ICSI) on unfertilized aged human oocytes. 
  • Expression of Sry, the mouse sex determining gene. 
  • Expression of a mitochondrial progesterone receptor in human spermatozoa correlates with a progestin-dependent increase in mitochondrial membrane potential. 
  • Fertilising ability of testicular spermatozoa. 
  • Flagellar motility requires the cAMP-dependent phosphorylation of a heat-stable NP-40-soluble 56 kd protein, axokinin. 
  • For sperm--is closer to the egg an advantage or liability? 
  • Germ cell aneuploidy in zebrafish with mutations in the mitotic checkpoint gene mps1. 
  • Glucocorticoid receptor binding to rat liver nuclei occurs without nuclear transport. 
  • Hamster sperm penetration of the zona pellucida: kinematic analysis and mechanical implications. 
  • Highly variable sperm precedence in the stalk-eyed fly, Teleopsis dalmanni. 
  • Human cervical mucus and its interaction with sperm: a fine-structural view. 
  • Human cervical mucus: research update. 
  • Human sperm penetration of zona-free hamster eggs after storage of the semen for 48 hours at 2 degrees C to 5 degrees C. 
  • Identification of a new variant of PDE1A calmodulin-stimulated cyclic nucleotide phosphodiesterase expressed in mouse sperm. 
  • Identification of an X-ray induced deletion mutant flanked by direct repeats. 
  • Identification, characterization, and functional correlation of calmodulin-dependent protein phosphatase in sperm. 
  • Immunohistochemical expression of monoclonal antibody 43-9F in testicular germ cell tumours. 
  • Impact of fertilization history and semen parameters on ICSI outcome. 
  • Improved motility recovery of human spermatozoa after freeze preservation via a new approach. 
  • In vitro studies of the golden hamster sperm acrosome reaction: completion on the zona pellucida and induction by homologous soluble zonae pellucidae. 
  • Increases in human sperm oxygen consumption at low cell concentrations. 
  • Isolation of a human teratoma cell line which expresses F9 antigen. 
  • Kinematic response of human spermatozoa to nonoxynol-9. 
  • Late intracytoplasmic sperm injection in unexpected failed fertilization in vitro: diagnostic or therapeutic? 
  • Location of the PH-20 protein on acrosome-intact and acrosome-reacted spermatozoa of cynomolgus macaques. 
  • Macrophages and infertility: enhancement of human macrophage-mediated sperm killing by antisperm antibodies. 
  • Macrophages and infertility: oviductal macrophages as potential mediators of infertility. 
  • Male germ cells and photoreceptors, both dependent on close cell-cell interactions, degenerate upon ClC-2 Cl(-) channel disruption. 
  • Measurement and modulation of nonoxynol-9 diffusion and bioactivity against spermatozoa in human cervical mucus. 
  • Mechanics of sperm-egg interaction at the zona pellucida. 
  • Mechanisms of filtration of morphologically abnormal human sperm by cervical mucus. 
  • Membrane remodelling during sperm maturation in the epididymis. 
  • Molecular dosimetry of the mutagen ethyl methanesulfonate in Drosophila melanogaster spermatozoa: linear relation of DNA alkylation per sperm cell (dose) to sex-linked recessive lethals. 
  • Molecular genetic analysis of two human sperm fibrous sheath proteins, AKAP4 and AKAP3, in men with dysplasia of the fibrous sheath. 
  • Morphometric analysis of spermatozoa in the assessment of human male fertility. 
  • Movement characteristics and acrosomal status of rabbit spermatozoa recovered at the site and time of fertilization. 
  • Movement characteristics and power output of guinea-pig and hamster spermatozoa in relation to activation. 
  • Movement characteristics of hamster and guinea pig spermatozoa upon attachment to the zona pellucida. 
  • Movement of cynomolgus and rhesus monkey spermatozoa collected from the lower female reproductive tract. 
  • Mutations produced by transmutation of phosphorus-32 to sulfur-32 within Drosophila DNA. 
  • NO is necessary and sufficient for egg activation at fertilization. 
  • Negative regulation of DNA replication by the retinoblastoma protein is mediated by its association with MCM7. 
  • New measures of sperm motion. I. Adaptive smoothing and harmonic analysis. 
  • Obesity-related DNA methylation at imprinted genes in human sperm: Results from the TIEGER study. 
  • Odorant receptors and desensitization proteins colocalize in mammalian sperm. 
  • Optimizing Nervous System-Specific Gene Targeting with Cre Driver Lines: Prevalence of Germline Recombination and Influencing Factors. 
  • Outcome of intracytoplasmic sperm injection in azoospermic patients: stressing the liaison between the urologist and reproductive medicine specialist. 
  • Outer dense fiber proteins are dominant postobstruction autoantigens in adult Lewis rats. 
  • P150c-abl is detected in mouse male germ cells by an in vitro kinase assay and is associated with stage-specific phosphoproteins in haploid cells. 
  • Percutaneous epididymal sperm aspiration and intracytoplasmic sperm injection in the management of infertility due to obstructive azoospermia. 
  • Permanent loss of male fecundity following sperm depletion in Aedes aegypti (L.). 
  • Phosphoproteome analysis of capacitated human sperm. Evidence of tyrosine phosphorylation of a kinase-anchoring protein 3 and valosin-containing protein/p97 during capacitation. 
  • Physiological implications of the presence, distribution, and regulation of calmodulin in eukaryotic cells. 
  • Polymerization of actin. IV. Role of Ca++ and H+ in the assembly of actin and in membrane fusion in the acrosomal reaction of echinoderm sperm. 
  • Preliminary examination of polymorphisms of GSTM1, GSTT1, and GSTZ1 in relation to semen quality. 
  • Redefining the relationship between sperm deoxyribonucleic acid fragmentation as measured by the sperm chromatin structure assay and outcomes of assisted reproductive techniques. 
  • Reflections on CASA after 25 years. 
  • Regulation of protein phosphorylation and motility of sperm by cyclic adenosine monophosphate and calcium. 
  • Relationships of mammalian sperm motility and morphology to hydrodynamic aspects of cell function. 
  • Relative biological effectiveness of tritiated water to gamma radiation for germ line mutations. 
  • Reversibility of sterilization produced by vas occlusion clip. 
  • SLLP1, a unique, intra-acrosomal, non-bacteriolytic, c lysozyme-like protein of human spermatozoa. 
  • Semen analysis. 
  • Semen characteristics of Vietnam veterans. 
  • Semen quality: is there a paternal effect on pregnancy outcome in in-vitro fertilization/intracytoplasmic sperm injection? 
  • Shifting gears and putting on the brakes: Female germ cells transition into meiosis. 
  • Simultaneous assessment of human sperm motility and morphology by videomicrography. 
  • Sperm DNA methylation altered by THC and nicotine: Vulnerability of neurodevelopmental genes with bivalent chromatin. 
  • Sperm morphology as diagnosed by strict criteria: probing the impact of teratozoospermia on fertilization rate and pregnancy outcome in a large in vitro fertilization population. 
  • Sperm phagocytosis by human peritoneal macrophages: a possible cause of infertility in endometriosis. 
  • Sperm transport in the reproductive tract of the female rabbit: II. The sustained phase of transport. 
  • Sperm-macrophage interaction in the mouse: a quantitative assay in vitro using 111indium oxine-labeled sperm. 
  • Spermiogenesis and exchange of basic nuclear proteins are impaired in male germ cells lacking Camk4. 
  • Stability of Drosophila chromosomes to radioactive decay of incorporated phosphorus-32. 
  • Standardization and comparability of CASA instruments. 
  • Stimulation of spermatogenesis with recombinant human follicle-stimulating hormone (follitropin alfa; GONAL-f): long-term treatment in azoospermic men with hypogonadotropic hypogonadism. 
  • Summarizing the motion of self-propelled cells: applications to sperm motility. 
  • Swimming of spermatozoa in a linear viscoelastic fluid. 
  • Synaptonemal complex complement of man in spreads of spermatocytes, with details of the sex chromosome pair. 
  • Synaptonemal complex karyotyping in spermatocytes of the Chinese hamster (Cricetulus griseus). III. Quantitative evaluation. 
  • The AZFc region of the Y chromosome features massive palindromes and uniform recurrent deletions in infertile men. 
  • The Role of a Mitochondrial Progesterone Receptor (PR-M) in Progesterone Action. 
  • The evolution of conspecific sperm precedence in Drosophila. 
  • The fine structure of spermatogenesis in Hymenolepis diminuta (Cestoda) with a description of the mature spermatozoon. 
  • The gene encoding bone morphogenetic protein 8B is required for the initiation and maintenance of spermatogenesis in the mouse. 
  • The healthy men study: an evaluation of exposure to disinfection by-products in tap water and sperm quality. 
  • The impact of obstructive interval and sperm granuloma on outcome of vasectomy reversal. 
  • The influence of large deletions on the mutation frequency induced by tritiated water and X-radiation in male Drosophila melanogaster post-meiotic germ cells. 
  • The mechanisms and analysis of sperm migration through cervical mucus. 
  • The physiologic consequences of vas ligation. 
  • The relation of computer-based measures of sperm morphology and motility to male infertility. 
  • The relationship between the motility and morphology of spermatozoa in human semen. 
  • Thresholds for thermal damage to normal tissues: an update. 
  • Timing of onset of contraceptive effectiveness in Norplant implant users. Part I. Changes in cervical mucus. 
  • Transmutation of carbon-14 within DNA of Drosophila melanogaster spermatozoa. 
  • Treatment of human spermatozoa with seminal plasma inhibits protein tyrosine phosphorylation. 
  • Use of human gametes obtained from anonymous donors for the production of human embryonic stem cell lines. 
  • Validity and cost-effectiveness of antisperm antibody testing before in vitro fertilization. 
  • Variations within and amongst normal men of movement characteristics of seminal spermatozoa. 
  • Vertebrate Reproduction. 
  • Water mobility within bovine cervical mucus. 
  • What functions of the sperm cell are measured by in vitro fertilization of zona-free hamster eggs? 
  • Y353/B: a candidate multiple-copy spermiogenesis gene on the mouse Y chromosome. 
  • cAMP-dependent regulatory processes in the acquisition and control of sperm flagellar movement. 
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  Keywords of People

  • Bennett, Vann, George Barth Geller Distinguished Professor of Molecular Biology, Duke Cancer Institute
  • Hogan, Brigid L. M., George Barth Geller Distinguished Professor for Research in Molecular Biology, Cell Biology
  • Katz, David F., Nello L. Teer, Jr. Distinguished Professor of Biomedical Engineering, in the Edmund T. Pratt, Jr. School of Engineering, Biomedical Engineering
  • Soderling, Scott Haydn, George Barth Geller Distinguished Professor of Molecular Biology, Cell Biology
  • Starmer, Charles Franklin, Professor Emeritus of Computer Science, Computer Science