Staining and Labeling

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  Subject Areas on Research

  • "Wildcatting" for breakthroughs in urothelial cancer detection and management--a frustrating business. 
  • A chemical method for labeling lysine methyltransferase substrates. 
  • A comparative histologic study of rapid three-dimensional zygomatic suture expansion osteogenesis. 
  • A comparative study of the effect of arterial and venous occlusion after various periods of ischemia. 
  • A determination of the outer dimensions of oncornaviruses by several electron microscopic procedures. 
  • A fixative for use in muscle histochemistry. 
  • A hybrid DNA aptamer-dendrimer nanomaterial for targeted cell labeling. 
  • A method for the continuous calculation of the age of labeled red blood cells. 
  • A mouse model of adult-onset anaemia due to erythropoietin deficiency. 
  • A multimodal micro-optrode combining field and single unit recording, multispectral detection and photolabeling capabilities. 
  • A new stain for systemic fungi in tissue. 
  • A novel approach for assessing protein synthesis in channel catfish, Ictalurus punctatus. 
  • A novel approach to the measurement of different in vitro leukaemic cell growth parameters: the use of PKH GL fluorescent probes. 
  • A projection from the parabigeminal nucleus to the pulvinar nucleus in Galago. 
  • A rapid method of staining ultrathin sections for surgical pathology TEM with the use of the microwave oven. 
  • A robust automated measure of average antibody staining in immunohistochemistry images. 
  • A study of pyramidal cell axon collaterals in intact and partially isolated adult cerebral cortex. 
  • A transgenic toolkit for visualizing and perturbing microtubules reveals unexpected functions in the epidermis. 
  • Active staining of mouse embryos for magnetic resonance microscopy. 
  • Air bubble-associated endothelial trauma in descemet stripping automated endothelial keratoplasty. 
  • Altered distribution of mitochondria and actin fibers in 3T3 cells cultured on microcarriers. 
  • An alpha hydroxy acid derivative suitable for sensitive skin. 
  • Antibody binding to a conformation-dependent epitope induces L-selectin association with the detergent-resistant cytoskeleton. 
  • Applied electric fields accelerate the diffusion rate and increase the diffusion distance of DiI in fixed tissue. 
  • Aptamer-mediated delivery of chemotherapy to pancreatic cancer cells. 
  • Assaying surface expression of chemosensory receptors in heterologous cells. 
  • Automated segmentation of the actively stained mouse brain using multi-spectral MR microscopy. 
  • Axons from non-cochlear sources in the anteroventral cochlear nucleus of the cat. A study with the rapid Golgi method. 
  • Basic methods in histopathology of joint tissues. 
  • Bioluminescence imaging to track bacterial dissemination of Yersinia pestis using different routes of infection in mice. 
  • Brain injury and protective effects of hypothermia using triphenyltetrazolium chloride in neonatal rat. 
  • Brain tumors induced in dogs by the Schmidt-Ruppin strain of Rous sarcoma virus. Neuropathological and immunological observations. 
  • Carbohydrate histochemistry of surface epithelium of rat trachea: backscatter and x-ray imaging. 
  • Cell adhesion molecule expression in coronary artery aneurysms in acute Kawasaki disease. 
  • Cellular senescence in the glaucomatous outflow pathway. 
  • Characterization of a novel human corneal endothelial antigen. 
  • Clonally dominant cardiomyocytes direct heart morphogenesis. 
  • Collagen-rich crystalloids in a scarred vascularized cornea. 
  • Comparison of acridine orange and Gram stains for detection of microorganisms in cerebrospinal fluid and other clinical specimens. 
  • Comparison of acridine orange, methylene blue, and Gram stains for blood cultures. 
  • Comparison of glycoproteins isolated by phenol-water partition of human erythrocyte membranes. 
  • Correlation between interferon- gamma secretion and cytotoxicity, in virus-specific memory T cells. 
  • Correlations of unique clinical, immunotypic, and histologic findings in cutaneous gamma/delta T-cell lymphoma. 
  • Decreased cochlear DNA receptor staining in MRL.MpJ-Fas(lpr) autoimmune mice with hearing loss. 
  • Demineralized Murine Skeletal Histology. 
  • Demineralized murine skeletal histology. 
  • Dendritic development of Purkinje and granule cells in the cerebellar cortex of rats treated postnatally with alpha-difluoromethylornithine. 
  • Dendritic properties of hippocampal CA1 pyramidal neurons in the rat: intracellular staining in vivo and in vitro. 
  • Descemet-stripping automated endothelial keratoplasty: effect of inserting forceps on DSAEK donor tissue viability by using an in vitro delivery model and vital dye assay. 
  • Design and characterization of microporous hyaluronic acid hydrogels for in vitro gene transfer to mMSCs 
  • Detection of Pneumocystis jiroveci in respiratory specimens by four staining methods. 
  • Detection of iron-labeled single cells by MR imaging based on intermolecular double quantum coherences at 14 T. 
  • Diagnosis of gastrointestinal T-cell lymphomas in routinely processed tissues. 
  • Differential uptake of DNA–poly (ethylenimine) polyplexes in cells cultured on collagen and fibronectin surfaces 
  • Disruption of endosperm development is a major cause of hybrid seed inviability between Mimulus guttatus and Mimulus nudatus. 
  • Distribution and localization of a novel cholecystokinin-releasing factor in the rat gastrointestinal tract. 
  • Distributions of choline acetyltransferase and acetylcholinesterase activities in the retinal layers of the red-tailed hawk and road runner. 
  • Ductular and proliferative response of esophageal submucosal glands in a porcine model of esophageal injury and repair. 
  • Early forming label-retaining muscle stem cells require p27kip1 for maintenance of the primitive state. 
  • Effects of n-alkanes on the morphology of lipid bilayers. A freeze-fracture and negative stain analysis. 
  • Encoded multichromophore response for simultaneous label-free detection. 
  • Enhanced T2 contrast for MR histology of the mouse brain. 
  • Enrichment of human bone marrow mononuclear phagocytes and characterization of macrophage subpopulations by immunoenzymatic double staining. 
  • Eosinophilic intracytoplasmic globules in pulmonary adenocarcinomas: a histochemical, immunohistochemical, and ultrastructural study of six cases. 
  • Epidermal growth factor receptor expression in normal and malignant endometrium. 
  • Expression of RON Proto-oncogene in Renal Oncocytoma and Chromophobe Renal Cell Carcinoma. 
  • Facilitated light microscopic cytochemical diagnosis of acute myelogenous leukemia. 
  • False-positive Gram-stained smears. 
  • Fenestrations of sarcoplasmic reticulum. Delineation by lanthanum acting as a fortuitous tracer and in situ negative stain. 
  • Fibrillar systems in cell motility. 
  • Fibrin assembly: a comparison of electron microscopic and light scattering results. 
  • Flow cytometric detection of lymphocyte proliferation in eyes with immunogenic inflammation. 
  • Fluorescein CorneoGraphy (FCG): Use of a repurposed fluorescein imaging technique to objectively standardize corneal staining. 
  • Fluorescent viability stains overestimate chondrocyte viability in osteoarticular allografts. 
  • Free radical scavenger specifically prevents ischemic focal ventricular tachycardia. 
  • Gating Harmonization Guidelines for Intracellular Cytokine Staining Validated in Second International Multiconsortia Proficiency Panel Conducted by Cancer Immunotherapy Consortium (CIC/CRI). 
  • Generalized papular xanthomatosis in mycosis fungoides. 
  • Genetic labeling of neurons in mouse brain. 
  • Giant two-dimensional gel electrophoresis: methodological update and comparison with intermediate-format gel systems. 
  • Hematoxylin: Mesoamerica's Gift to Histopathology. Palo de Campeche (Logwood Tree), Pirates' Most Desired Treasure, and Irreplaceable Tissue Stain. 
  • Hepatocyte paraffin 1 antigen as a biomarker for early diagnosis of Barrett esophagus. 
  • Hereditary benign intraepithelial dyskeratosis: an evaluation of diagnostic cytology. 
  • High-resolution in vivo imaging of blood vessels without labeling. 
  • Highly photostable fluorescent labeling of proteins in live cells using exchangeable coiled coils heterodimerization. 
  • Histologic observations and P-glycoprotein expression in gastric and esophageal adenocarcinomas treated with preoperative chemotherapy. 
  • Histologic, nuclear DNA, and human papillomavirus studies of cervical condylomas. 
  • Histopathologic features of adult-onset foveomacular pigment epithelial dystrophy. 
  • Human Adipose-Derived Stem Cells Labeled with Plasmonic Gold Nanostars for Cellular Tracking and Photothermal Cancer Cell Ablation. 
  • Human acute leukemia cells with membrane-bound immunoglobulin. 
  • Human aortic fibrolipid lesions. Immunochemical localization of apolipoprotein B and apolipoprotein A. 
  • Human conjunctival surface mucins: a quantitative study of normal and diseased (KCS) tissue. 
  • Identification and visualization of multidimensional antigen-specific T-cell populations in polychromatic cytometry data. 
  • Identification of calcium oxalate crystals using alizarin red S stain. 
  • Identification of personal lubricants that can cause rectal epithelial cell damage and enhance HIV type 1 replication in vitro. 
  • Imaging Escherichia coli using functionalized core/shell CdSe/CdS quantum dots. 
  • Imaging synaptic inhibition in transgenic mice expressing the chloride indicator, Clomeleon. 
  • Immunohistochemical expression of monoclonal antibody 43-9F in testicular germ cell tumours. 
  • Immunohistochemical localization of epidermal growth factor in cat paradental tissues during tooth movement. 
  • Immunohistochemical p16INK4a analysis of archival tumors with deletion, hypermethylation, or mutation of the CDKN2/MTS1 gene. A comparison of four commercial antibodies. 
  • Immunohistologic quantification of Pseudomonas aeruginosa in the tracheobronchial tree from patients with cystic fibrosis. 
  • Improved techniques for staining and enumerating focus formation in viral infectivity assays. 
  • In vivo analysis of white adipose tissue in zebrafish. 
  • In vivo bioluminescent imaging of influenza a virus infection and characterization of novel cross-protective monoclonal antibodies. 
  • In vivo fluorescein staining of SI-30NB silicone intraocular lens. 
  • In vivo staining of cytoskeletal actin by autointernalization of nontoxic concentrations of nitrobenzoxadiazole-phallacidin. 
  • Incorporation of low molecular weight molecules into alpha(2)-macroglobulin by nucleophilic exchange. 
  • Infantile chlamydial conjunctivitis. A comparison of Papanicolaou, Giemsa and immunoperoxidase staining methods. 
  • Infectious disease pathology. 
  • Injection of Hydrogel Biomaterial Scaffolds to The Brain After Stroke. 
  • Inositol pyrophosphates modulate S phase progression after pheromone-induced arrest in Saccharomyces cerevisiae. 
  • Insulin-like growth factor I in skeletal muscle after weight-lifting exercise in frail elders. 
  • Interpathologist Diagnostic Agreement for Non-Small Cell Lung Carcinomas Using Current and Recent Classifications. 
  • Junctions between the cells of the trabecular meshwork. 
  • Label-Free Imaging of Female Genital Tract Melanocytic Lesions With Pump-Probe Microscopy: A Promising Diagnostic Tool. 
  • Label-free measurement of microbicidal gel thickness using low-coherence interferometry. 
  • Label-free, high-throughput measurements of dynamic changes in cell nuclei using angle-resolved low coherence interferometry. 
  • Laser-capture microdissection. 
  • Lectin staining of cultured CNS microglia. 
  • Leiomyosarcoma of the kidney: a clinicopathologic study. 
  • Localization of RNA tumor virus polypeptides. I. Isolation of further virus substructures. 
  • Localization of cell wall polysaccharides in nonarticulated laticifers of Asclepias speciosa Torr. 
  • Loss of hippocampal acetylcholinesterase staining after fornix lesion in the monkey. 
  • Lymphatic mapping and sentinel lymph node biopsy in patients with melanoma: a meta-analysis. 
  • Macrophage imaging within human cerebral aneurysms wall using ferumoxytol-enhanced MRI: a pilot study. 
  • Macrophage infiltration in the human retina. 
  • Mechanism of detection of acute cerebral ischemia in rats by diffusion-weighted magnetic resonance microscopy. 
  • Metabolic glycan labeling and chemoselective functionalization of native biomaterials. 
  • Metabolic labeling enables selective photocrosslinking of O-GlcNAc-modified proteins to their binding partners. 
  • Method for decellularizing skeletal muscle without detergents or proteolytic enzymes. 
  • Microbial biofilms in the gut: visualization by electron microscopy and by acridine orange staining. 
  • Microscopic diffusion tensor imaging of the mouse brain. 
  • Mitomycin-induced hemolytic-uremic syndrome. 
  • Mitral valvular interstitial cell responses to substrate stiffness depend on age and anatomic region. 
  • Modeling the developmental neurotoxicity of nicotine in vitro: cell acquisition, growth and viability in PC12 cells. 
  • Molecular and genetic approaches for the analysis of C. elegans neuronal development. 
  • Morphometric analysis of spermatozoa in the assessment of human male fertility. 
  • Multicolor in vitro translation. 
  • Multicolor mapping of the cardiomyocyte proliferation dynamics that construct the atrium. 
  • Multidimensional Tracking of GPCR Signaling via Peroxidase-Catalyzed Proximity Labeling. 
  • Murine granulocyte-macrophage colony-stimulating factor expressed from a bicistronic simian immunodeficiency virus-based integrase-defective lentiviral vector does not enhance T-cell responses in mice. 
  • Naïve T cells are maintained in the periphery during the first 3 months of acute HIV-1 infection: implications for analysis of thymus function. 
  • Neural cell adhesion molecule-secreting transgenic mice display abnormalities in GABAergic interneurons and alterations in behavior. 
  • Neural circuits. Labeling of active neural circuits in vivo with designed calcium integrators. 
  • Neurobehavioral damage to cholinergic systems caused by prenatal exposure to heroin or phenobarbital: cellular mechanisms and the reversal of deficits by neural grafts. 
  • Neuroendocrine differentiation is an independent prognostic factor in chemotherapy-treated nonsmall cell lung carcinoma. 
  • Novel in vitro gene transfer method for study of local modulators in vascular smooth muscle cells. 
  • On gap junction structure. 
  • Organization of the superior olivary complex in the guinea pig. I. Cytoarchitecture, cytochrome oxidase histochemistry, and dendritic morphology. 
  • Orientation of acidic polysaccharides and rhodopsin-oligosaccharides in frog retinal rod outer segments. 
  • Orthogonal site-specific protein modification by engineering reversible thiol protection mechanisms. 
  • Overexpression of p53 is not a feature of benign and early-stage borderline epithelial ovarian tumors. 
  • Pathways connecting the right and left cochlear nuclei. 
  • Phenotypic and functional characterization of cytotoxic T lymphocytes by flow cytometry. 
  • Phenotypic stability of Pro347Leu rhodopsin transgenic pigs as indicated by photoreceptor cell degeneration. 
  • Physiological organization of layer 4 in macaque striate cortex. 
  • Ploidy analysis of epithelial ovarian cancers using image cytometry. 
  • Point sources of Schwann cells result in growth into a nerve entubulation repair site in the absence of axons: effects of freeze-thawing. 
  • Polypeptides of avian RNA tumor viruses. 1. Isolation and physical and chemical analysis. 
  • Polypeptides of avian RNA tumor viruses. II. Serological characterization. 
  • Possible extrathymic development of nonfunctional T cells in a patient with complete DiGeorge syndrome. 
  • Preferential sputtering effects in the ion microanalysis of biological tissues. 
  • Pressure effects on the distribution of extracellular materials in the rhesus monkey outflow apparatus. 
  • Proliferation index in various stages of breast cancer determined by Ki-67 immunostaining. 
  • Properties of mouse leukemia viruses. II. Isolation of viral components. 
  • Protease-activated quantum dot probes. 
  • Protein polymer hydrogels by in situ, rapid and reversible self-gelation. 
  • Quantitative neuromorphometry using magnetic resonance histology. 
  • Rapid histological staining procedures for material from immune-suppressed patients. 
  • Rapid staining and imaging of subnuclear features to differentiate between malignant and benign breast tissues at a point-of-care setting. 
  • Real-time imaging of morphogenetic movements in Drosophila using Gal4-UAS-driven expression of GFP fused to the actin-binding domain of moesin. 
  • Recent advances for measurement of protein synthesis rates, use of the 'Virtual Biopsy' approach, and measurement of muscle mass. 
  • Recent and innovative methods for detection of bacteremia and fungemia. 
  • Recurrent demyelinating myelitis associated with hepatitis C viral infection. 
  • Recurrent mossy fiber pathway in rat dentate gyrus: synaptic currents evoked in presence and absence of seizure-induced growth. 
  • Regenerating titanium ventricular assist device surfaces after gold/palladium coating for scanning electron microscopy. 
  • Regular structures in unit membranes. III. Further observations on the particulate component of the suckling rat ileum endocytic membrane complex. 
  • Regulation of ZAP-70 intracellular localization: visualization with the green fluorescent protein. 
  • Rejection criteria for endotracheal aspirates from adults. 
  • Relationship between 3T3 cell spreading and the strength of adhesion on glass and silane surfaces. 
  • Relationship of DNA ploidy to histology and prognosis in rhabdomyosarcoma. Comparison of flow cytometry and image analysis. 
  • Reproducibility of left atrial ablation with high-intensity focused ultrasound energy in a calf model. 
  • Robust approaches to quantitative ratiometric FRET imaging of CFP/YFP fluorophores under confocal microscopy. 
  • Role of platelet activating factor in the inflammatory and secretory effects of Clostridium difficile toxin A. 
  • Sedimentation and volume analysis of human bone marrow. 
  • Segmentation of the canine corpus callosum using diffusion-tensor imaging tractography. 
  • Selective neuronal death after transient forebrain ischemia in the Mongolian gerbil: a silver impregnation study. 
  • Silver staining of histones in Triton-acid-urea gels. 
  • Simple identification of the third segment of the extracranial vertebral artery by extreme lateral inferior transcondylar-transtubercular exposure (ELITE). 
  • Small leucine rich repeat proteoglycans (SLRPs) in the human sclera: identification of abundant levels of PRELP. 
  • Solid-phase capture for the detection and relative quantification of S-nitrosoproteins by mass spectrometry. 
  • Somatostatin immunohistochemistry of hippocampal slices with lucifer yellow-stained pyramidal neurons responding to somatostatin. 
  • Specialized vascularization of the primate visual cortex. 
  • Staining methods for magnetic resonance microscopy of the rat fetus. 
  • Statistical analysis of the concordance of immunohistochemical stains with the final diagnosis in spitzoid neoplasms. 
  • Steady-state fluorescence imaging of neoplasia. 
  • Structural features of the terminal loop region of frog retinal rod outer segment disk membranes: II. Organization of the terminal loop complex. 
  • Studies on cellular autophagocytosis. A histochemical study on sequential alterations of mitochondria in the glucagon-induced autophagic vacuoles of rat liver. 
  • Studies on cellular autophagocytosis. Cyclic AMP- and dibutyryl cyclic AMP-stimulated autophagy in rat liver. 
  • Sympathohippocampal neurons are inside the blood--brain barrier. 
  • Synthesis of N-succinimidyl 4-guanidinomethyl-3-[*I]iodobenzoate: a radio-iodination agent for labeling internalizing proteins and peptides. 
  • TRPV4 channels augment macrophage activation and ventilator-induced lung injury. 
  • Targeted alpha-particle radiotherapy with 211At-labeled monoclonal antibodies. 
  • The Ca, Pi, F, and proline content of developing bovine enamel under GBHA-stained and unstained bands. 
  • The administration of renoprotective agents extends warm ischemia in a rat model. 
  • The appearance of the outflow apparatus of the eye after staining with ruthenium red. 
  • The application of permanent middle cerebral artery ligation in the mouse. 
  • The choice between the positive and negative structures for nerve myelin. 
  • The diagnosis of desmoplastic malignant mesothelioma and its distinction from fibrous pleurisy: a histologic and immunohistochemical analysis of 31 cases including p53 immunostaining. 
  • The differential expression of statin in the nuclei of human colonic crypts adjacent to a cancer: an immunohistochemical study. 
  • The effects of local progesterone on stilbestrol-associated vaginal adenosis. 
  • The proximity-labeling technique BioID identifies sorting nexin 6 as a member of the insulin-like growth factor 1 (IGF1)-IGF1 receptor pathway. 
  • The tumor biology of melanoma nodal metastases. 
  • The use of flow cytometry as a tool for monitoring filament formation of fungi. 
  • The use of histidase for the study of amniotic cell origin and histidinemia. 
  • The ventral nucleus of the lateral lemniscus of the gerbil (Meriones unguiculatus): organization of connections with the cochlear nucleus and the inferior colliculus. 
  • Tinctorial and morphologic properties distinguishing actinomycosis and nocardiosis. 
  • Tissue clearing techniques for three-dimensional optical imaging of intact human prostate and correlations with multi-parametric MRI. 
  • Transgenic expression of pancreatic secretory trypsin inhibitor-I ameliorates secretagogue-induced pancreatitis in mice. 
  • Transsynaptic Tracing from Peripheral Targets with Pseudorabies Virus Followed by Cholera Toxin and Biotinylated Dextran Amines Double Labeling. 
  • Two-dimensional rhodopsin crystals from disk membranes of frog retinal rod outer segments. 
  • Ultrastructural discrimination of lipid droplets and vesicles in atherosclerosis: value of osmium-thiocarbohydrazide-osmium and tannic acid-paraphenylenediamine techniques. 
  • Use of excitatory amino acids to make axon-sparing lesions of hypothalamus. 
  • Validation of interobserver agreement in lung cancer assessment: hematoxylin-eosin diagnostic reproducibility for non-small cell lung cancer: the 2004 World Health Organization classification and therapeutically relevant subsets. 
  • Variable presentation of nemaline myopathy: novel mutation of alpha actin gene. 
  • Waxholm space: an image-based reference for coordinating mouse brain research. 
  • p53 and MDM2 immunostaining in pulmonary blastomas and bronchogenic carcinomas. 
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  Keywords of People

  • Berchuck, Andrew, James M. Ingram Distinguished Professor of Gynecologic Oncology, Obstetrics and Gynecology, Gynecologic Oncology
  • Ferrari, Guido, Professor in Surgery, Molecular Genetics and Microbiology
  • Johnson, G. Allan, Charles E. Putman University Distinguished Professor of Radiology, Biomedical Engineering
  • Stinnett, Sandra Sue, Associate Professor of Biostatistics & Bioinformatics, Biostatistics & Bioinformatics
  • Turner, Dennis Alan, Professor of Neurosurgery, Biomedical Engineering
  • Wax, Adam P., Professor of Biomedical Engineering, Biomedical Engineering
  • White, Leonard Edward, Associate Professor in Neurology, Psychology & Neuroscience