Substrate Specificity

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  Subject Areas on Research

  • 3'-Azido-3'-deoxythymidine triphosphate as an inhibitor and substrate of purified human immunodeficiency virus reverse transcriptase. 
  • 3,4-Diaryl-5-hydroxyfuranones: highly selective inhibitors of cyclooxygenase-2 with aqueous solubility. 
  • A CDC6 protein-binding peptide selected using a bacterial two-hybrid-like system is a cell cycle inhibitor. 
  • A Fluorometric Method of Measuring Carboxypeptidase Activities for Angiotensin II and Apelin-13. 
  • A bacterial selection for the directed evolution of pyruvate aldolases. 
  • A chemical glycoproteomics platform reveals O-GlcNAcylation of mitochondrial voltage-dependent anion channel 2. 
  • A chemical method for labeling lysine methyltransferase substrates. 
  • A conserved family of enzymes that phosphorylate inositol hexakisphosphate. 
  • A continuous fluorometric assay for the feline immunodeficiency virus protease. 
  • A facile synthesis of 1-O-alkyl-2-(R)-hydroxypropane-3-phosphonocholine (lyso-phosphono-platelet activating factor). 
  • A loss-of-function RNA interference screen for molecular targets in cancer. 
  • A metabolic enzyme for S-nitrosothiol conserved from bacteria to humans. 
  • A modular switch for spatial Ca2+ selectivity in the calmodulin regulation of CaV channels. 
  • A mutation-induced activated state of the beta 2-adrenergic receptor. Extending the ternary complex model. 
  • A network of substrates of the E3 ubiquitin ligases MDM2 and HUWE1 control apoptosis independently of p53. 
  • A novel approach for characterizing protein ligand complexes: molecular basis for specificity of small-molecule Bcl-2 inhibitors. 
  • A phosphotransferase that generates phosphatidylinositol 4-phosphate (PtdIns-4-P) from phosphatidylinositol and lipid A in Rhizobium leguminosarum. A membrane-bound enzyme linking lipid a and ptdins-4-p biosynthesis. 
  • A protein microarray-based analysis of S-nitrosylation. 
  • A role for receptor kinases in the regulation of class II G protein-coupled receptors. Phosphorylation and desensitization of the secretin receptor. 
  • A single base substitution in the variable pocket of yeast tRNA(Arg) eliminates species-specific aminoacylation. 
  • A spectrophotometric glutathione S-transferase assay displaying alpha-class selectivity utilizing 1-p-chlorophenyl-4,4-dimethyl-5-diethylamino-1-penten-3- one hydrobromide. 
  • A structural basis for lithium and substrate binding of an inositide phosphatase. 
  • A substrate specific functional polymorphism of human gamma-glutamyl hydrolase alters catalytic activity and methotrexate polyglutamate accumulation in acute lymphoblastic leukaemia cells. 
  • A systems genetics approach identifies Trp53inp2 as a link between cardiomyocyte glucose utilization and hypertrophic response. 
  • A ubiquitin E2 variant protein acts in axon termination and synaptogenesis in Caenorhabditis elegans. 
  • A viral microRNA functions as an orthologue of cellular miR-155. 
  • AQP2 is a substrate for endogenous PP2B activity within an inner medullary AKAP-signaling complex. 
  • Abi-2, a novel SH3-containing protein interacts with the c-Abl tyrosine kinase and modulates c-Abl transforming activity. 
  • Activated human plasma carboxypeptidase B is retained in the blood by binding to alpha2-macroglobulin and pregnancy zone protein. 
  • Acyl carrier protein-specific 4'-phosphopantetheinyl transferase activates 10-formyltetrahydrofolate dehydrogenase. 
  • Adding a positive charge at residue 46 of Drosophila alcohol dehydrogenase increases cofactor specificity for NADP+. 
  • Adducin is an in vivo substrate for protein kinase C: phosphorylation in the MARCKS-related domain inhibits activity in promoting spectrin-actin complexes and occurs in many cells, including dendritic spines of neurons. 
  • Affinity of (alpha-P-borano)-NTP analogs to rabbit muscle pyruvate kinase. 
  • Allosteric activation of proto-oncogene kinase Src by GPCR-beta-arrestin complexes. 
  • Altered intracellular and extracellular signaling leads to impaired T-cell functions in ADA-SCID patients. 
  • Aminonaphthalenesulfonamides, a new class of modifiable fluorescent detecting groups and their use in substrates for serine protease enzymes. 
  • An EcoRI-RsrI chimeric restriction endonuclease retains parental sequence specificity. 
  • An approach to the study of G-protein-coupled receptor kinases: an in vitro-purified membrane assay reveals differential receptor specificity and regulation by G beta gamma subunits. 
  • An economical and preparative orthogonal solid phase synthesis of fluorescein and rhodamine derivatized peptides: FRET substrates for the Staphylococcus aureus sortase SrtA transpeptidase reaction. 
  • Analysis of medaka cytochrome P450 3A homotropic and heterotropic cooperativity. 
  • Analysis of recombinant Phex: an endopeptidase in search of a substrate. 
  • Analysis of the substrate specificity of the Staphylococcus aureus sortase transpeptidase SrtA. 
  • Ankyrin regulation: an alternatively spliced segment of the regulatory domain functions as an intramolecular modulator. 
  • Apparent suicide inactivation of human lymphoblast S-adenosylhomocysteine hydrolase by 2'-deoxyadenosine and adenine arabinoside. A basis for direct toxic effects of analogs of adenosine. 
  • Application of chemoproteomics to drug discovery: identification of a clinical candidate targeting hsp90. 
  • Association between ratio of matrix metalloproteinase-1 to tissue inhibitor of metalloproteinase-1 and local recurrence, metastasis, and survival in human chondrosarcoma. 
  • Autoinhibition of casein kinase I epsilon (CKI epsilon) is relieved by protein phosphatases and limited proteolysis. 
  • Autoregulation of enzymes by pseudosubstrate prototopes: myosin light chain kinase. 
  • Beta 2-adrenergic receptor stimulated, G protein-coupled receptor kinase 2 mediated, phosphorylation of ribosomal protein P2. 
  • Beta-adrenergic receptor kinase: primary structure delineates a multigene family. 
  • Binding of tissue-type plasminogen activator to the glucose-regulated protein 78 (GRP78) modulates plasminogen activation and promotes human neuroblastoma cell proliferation in vitro. 
  • Biochemical analysis of inositol phosphate kinases. 
  • Biochemical role of the Cryptococcus neoformans ADE2 protein in fungal de novo purine biosynthesis. 
  • C-terminal repeat domain kinase I phosphorylates Ser2 and Ser5 of RNA polymerase II C-terminal domain repeats. 
  • CD10/neutral endopeptidase 24.11 hydrolyzes bombesin-like peptides and regulates the growth of small cell carcinomas of the lung. 
  • CD19 regulates Src family protein tyrosine kinase activation in B lymphocytes through processive amplification. 
  • CD9 plays a role in Schwann cell migration in vitro. 
  • CYP3A probes can quantitatively predict the in vivo kinetics of other CYP3A substrates and can accurately assess CYP3A induction and inhibition. 
  • Ca(2+)-regulated serine protease associated with the nuclear scaffold. 
  • Caged protein prenyltransferase substrates: tools for understanding protein prenylation. 
  • Calmodulin-dependent multifunctional protein kinase. Evidence for isoenzyme forms in mammalian tissues. 
  • Caspase cleavage is not for everyone. 
  • Caspase-3-dependent cleavage of Bcl-2 promotes release of cytochrome c. 
  • Caspase-8 levels affect necessity for mitochondrial amplification in death ligand-induced glioma cell apoptosis. 
  • Catalytic site remodelling of the DOT1L methyltransferase by selective inhibitors. 
  • Cell-type-dependent histone demethylase specificity promotes meiotic chromosome condensation in Arabidopsis. 
  • Chapter Nine - Cellular Roles of Beta-Arrestins as Substrates and Adaptors of Ubiquitination and Deubiquitination. 
  • Characterization of Ca2+/calmodulin-dependent protein kinase IV. Role in transcriptional regulation. 
  • Characterization of SARS2 Nsp15 nuclease activity reveals it's mad about U. 
  • Characterization of a Na+/glucose cotransporter cloned from rabbit small intestine. 
  • Characterization of additional components of the environmental pH-sensing complex in the pathogenic fungus Cryptococcus neoformans. 
  • Characterization of key residues in the subdomain encoded by exons 8 and 9 of human inducible nitric oxide synthase: a critical role for Asp-280 in substrate binding and subunit interactions. 
  • Characterization of neuronal protein phosphatases in Aplysia californica. 
  • Characterization of prorenin activation using a synthetic peptide substrate. 
  • Characterization of reaction intermediates of human excision repair nuclease. 
  • Characterization of the DNase I hypersensitive site 3' of the human beta globin gene domain. 
  • Characterization of the role of the amino-terminal proline in the enzymatic activity catalyzed by macrophage migration inhibitory factor. 
  • Choline Acetyltransferase Mutations Causing Congenital Myasthenic Syndrome: Molecular Findings and Genotype-Phenotype Correlations. 
  • Chromogenic assay for phospholipase C from Bacillus cereus. 
  • Cleavage of oligoribonucleotides by a ribozyme derived from the hepatitis delta virus RNA sequence. 
  • Cloning and characterization of PDE7B, a cAMP-specific phosphodiesterase. 
  • Cloning, isolation and characterization of the Thermotoga maritima KDPG aldolase. 
  • Cocrystal structure of protein farnesyltransferase complexed with a farnesyl diphosphate substrate. 
  • Collagenase specificity in chondrosarcoma metastasis. 
  • Colocalizing ribozymes with substrate RNAs to increase their efficacy as gene inhibitors. 
  • Comparative analysis of the ATP-binding sites of Hsp90 by nucleotide affinity cleavage: a distinct nucleotide specificity of the C-terminal ATP-binding site. 
  • Comparative properties of channel catfish (Ictalurus punctatus) and blue crab (Callinectes sapidus) acetylcholinesterases. 
  • Comparison of acceptor and donor substrates in the CoA-independent transacylase reaction in human neutrophils. 
  • Complex drug interactions of HIV protease inhibitors 1: inactivation, induction, and inhibition of cytochrome P450 3A by ritonavir or nelfinavir. 
  • Computational de novo design and characterization of a protein that selectively binds a highly hyperpolarizable abiological chromophore. 
  • Computational design and elaboration of a de novo heterotetrameric alpha-helical protein that selectively binds an emissive abiological (porphinato)zinc chromophore. 
  • Computational design of receptor and sensor proteins with novel functions. 
  • Computational design of receptors for an organophosphate surrogate of the nerve agent soman. 
  • Computational design, synthesis and biological evaluation of para-quinone-based inhibitors for redox regulation of the dual-specificity phosphatase Cdc25B. 
  • Concerted proton transfer mechanism of Clostridium thermocellum ribose-5-phosphate isomerase. 
  • Conversion of Tyr361 beta to Leu in mammalian protein farnesyltransferase impairs product release but not substrate recognition. 
  • Conversion of a maltose receptor into a zinc biosensor by computational design. 
  • Conversion of protein farnesyltransferase to a geranylgeranyltransferase. 
  • Conversion of protein phosphatase 1 catalytic subunit to a Mn(2+)-dependent enzyme impairs its regulation by inhibitor 1. 
  • Cross-clade inhibition of HIV-1 replication and cytopathology by using RNase P-associated external guide sequences. 
  • Crucial role of copper in detection of metal-coordinating odorants. 
  • Cryo-EM structure of human rhodopsin bound to an inhibitory G protein. 
  • Crystal structure of farnesyl protein transferase complexed with a CaaX peptide and farnesyl diphosphate analogue. 
  • Crystal structures of Toxoplasma gondii HGXPRTase reveal the catalytic role of a long flexible loop. 
  • Crystal structures of Toxoplasma gondii adenosine kinase reveal a novel catalytic mechanism and prodrug binding. 
  • Crystal structures of Toxoplasma gondii uracil phosphoribosyltransferase reveal the atomic basis of pyrimidine discrimination and prodrug binding. 
  • Crystal structures of the anticancer clinical candidates R115777 (Tipifarnib) and BMS-214662 complexed with protein farnesyltransferase suggest a mechanism of FTI selectivity. 
  • Crystallographic analysis of CaaX prenyltransferases complexed with substrates defines rules of protein substrate selectivity. 
  • DNA breakage and closure by rat liver type 1 topoisomerase: separation of the half-reactions by using a single-stranded DNA substrate. 
  • DNA determinants important in sequence recognition by Eco RI endonuclease. 
  • DNA strand breakage by wheat germ type 1 topoisomerase. 
  • DNA-dependent activation of the hMutSalpha ATPase. 
  • Density-fragment interaction approach for quantum-mechanical/molecular-mechanical calculations with application to the excited states of a Mg(2+)-sensitive dye. 
  • Dephosphorylation of distinct sites on microtubule-associated protein MAP1B by protein phosphatases 1, 2A and 2B. 
  • Dephosphorylation of the beta 2-adrenergic receptor and rhodopsin by latent phosphatase 2. 
  • Destabilization of the VCP-Ufd1-Npl4 complex is associated with decreased levels of ERAD substrates. 
  • Determination of separate inhibitor and substrate binding sites in the dehaloperoxidase-hemoglobin from Amphitrite ornata. 
  • Development of a high-throughput screening assay for inhibitors of small ubiquitin-like modifier proteases. 
  • Development of human protein reference database as an initial platform for approaching systems biology in humans. 
  • Development of magnetic resonance imaging contrast material for in vivo mapping of tissue transglutaminase activity. 
  • Development of peptide antagonists that target estrogen receptor-cofactor interactions. 
  • Dicoumarol-sensitive NADPH: phenanthrenequinone oxidoreductase in channel catfish (Ictalurus punctatus). 
  • Differential specificities and simultaneous occupancy of human MutSalpha nucleotide binding sites. 
  • Directed evolution of a new catalytic site in 2-keto-3-deoxy-6-phosphogluconate aldolase from Escherichia coli. 
  • Directed evolution of recombinase specificity by split gene reassembly. 
  • Discovery and characterization of human antibody inhibitors of pregnancy-associated plasma protein-A. 
  • Discovery and characterization of small molecules that target the GTPase Ral. 
  • Discovery of a cardiolipin synthase utilizing phosphatidylethanolamine and phosphatidylglycerol as substrates. 
  • Discrimination between right and wrong purine dNTPs by DNA polymerase I from Bacillus stearothermophilus. 
  • Disruption of a topoisomerase-DNA cleavage complex by a DNA helicase. 
  • Dissociation of bacteriophage T4 DNA polymerase and its processivity clamp after completion of Okazaki fragment synthesis. 
  • Distinct stress responses of two functional laccases in Cryptococcus neoformans are revealed in the absence of the thiol-specific antioxidant Tsa1. 
  • Downregulation of microglial activation by apolipoprotein E and apoE-mimetic peptides. 
  • Duration of cytochrome P-450 2E1 (CYP2E1) inhibition and estimation of functional CYP2E1 enzyme half-life after single-dose disulfiram administration in humans. 
  • Durhamycin A, a potent inhibitor of HIV Tat transactivation. 
  • E1-like activating enzyme Atg7 is preferentially sequestered into p62 aggregates via its interaction with LC3-I. 
  • Emergence of factor IXa as a target for pharmacologic inhibition: editors page. 
  • Emi2-mediated inhibition of E2-substrate ubiquitin transfer by the anaphase-promoting complex/cyclosome through a D-box-independent mechanism. 
  • Endonucleolytic function of MutLalpha in human mismatch repair. 
  • Engineering the substrate specificity of Staphylococcus aureus Sortase A. The beta6/beta7 loop from SrtB confers NPQTN recognition to SrtA. 
  • Enhancing mechanical properties of tissue-engineered constructs via lysyl oxidase crosslinking activity. 
  • Enzymatic characterization of class I DAD1-like acylhydrolase members targeted to chloroplast in Arabidopsis. 
  • Enzymatic generation of the amino terminus of the beta-amyloid peptide. 
  • Enzymatic modification of proteins with a geranylgeranyl isoprenoid. 
  • Enzymes of ceramide biosynthesis. 
  • Enzymology of long-chain base synthesis by liver: characterization of serine palmitoyltransferase in rat liver microsomes. 
  • Error-prone replication of oxidatively damaged DNA by a high-fidelity DNA polymerase. 
  • Escherichia coli mutS-encoded protein binds to mismatched DNA base pairs. 
  • Ether lipid metabolism by AADACL1 regulates platelet function and thrombosis. 
  • Experimental validation of the docking orientation of Cdc25 with its Cdk2-CycA protein substrate. 
  • Expression and site-directed mutagenesis of the phosphatidylcholine-preferring phospholipase C of Bacillus cereus: probing the role of the active site Glu146. 
  • Expression cloning of three Rhizobium leguminosarum lipopolysaccharide core galacturonosyltransferases. 
  • Expression of a variant form of the glutamate transporter GLT1 in neuronal cultures and in neurons and astrocytes in the rat brain. 
  • Expression of human protein phosphatase-1 in Saccharomyces cerevisiae highlights the role of phosphatase isoforms in regulating eukaryotic functions. 
  • Expression, purification, and identification of associated proteins of the full-length hCDK12/CyclinK complex. 
  • Extensive phosphorylation with overlapping specificity by Mycobacterium tuberculosis serine/threonine protein kinases. 
  • Extrinsic pathway proteolytic activity. 
  • FMRP targets distinct mRNA sequence elements to regulate protein expression. 
  • Facilitated diffusion during catalysis by EcoRI endonuclease. Nonspecific interactions in EcoRI catalysis. 
  • Farnesylation of YDJ1p is required for function at elevated growth temperatures in Saccharomyces cerevisiae. 
  • Farnesylation of nonpeptidic thiol compounds by protein farnesyltransferase. 
  • From promiscuity to precision: protein phosphatases get a makeover. 
  • Functional evaluation of nonphagocytic NAD(P)H oxidases. 
  • Functional evolution of an anthocyanin pathway enzyme during a flower color transition. 
  • Further Advances in Optimizing (2-Phenylcyclopropyl)methylamines as Novel Serotonin 2C Agonists: Effects on Hyperlocomotion, Prepulse Inhibition, and Cognition Models. 
  • G protein-coupled receptor kinases. 
  • G-protein-coupled receptors: regulatory role of receptor kinases and arrestin proteins. 
  • GRP94-associated enzymatic activities. Resolution by chromatographic fractionation. 
  • GTPase activating specificity of RGS12 and binding specificity of an alternatively spliced PDZ (PSD-95/Dlg/ZO-1) domain. 
  • Generation of an anticoagulant aptamer that targets factor V/Va and disrupts the FVa-membrane interaction in normal and COVID-19 patient samples. 
  • Genetically engineered calmodulins differentially activate target enzymes. 
  • Gibberellin-induced DELLA recognition by the gibberellin receptor GID1. 
  • GlcNAc-1-P-transferase-tunicamycin complex structure reveals basis for inhibition of N-glycosylation. 
  • Global Identification of Small Ubiquitin-related Modifier (SUMO) Substrates Reveals Crosstalk between SUMOylation and Phosphorylation Promotes Cell Migration. 
  • Glutathione content and glutathione-S-transferase expression in 1,3-bis(2-chloroethyl)-1-nitrosourea-resistant human malignant astrocytoma cell lines. 
  • Granulocyte-angiotensin system. Identification of angiotensinogen as the plasma protein substrate of leukocyte cathepsin G. 
  • Guanidine-induced equilibrium unfolding of a homo-hexameric enzyme 4-oxalocrotonate tautomerase (4-OT). 
  • H-Ras peptide and protein substrates bind protein farnesyltransferase as an ionized thiolate. 
  • HCPTPA, a protein tyrosine phosphatase that regulates vascular endothelial growth factor receptor-mediated signal transduction and biological activity. 
  • HER-2/c-erbB2 is phosphorylated by calmodulin-dependent protein kinase II on a single site in the cytoplasmic tail at threonine-1172. 
  • Hepatic cytochrome P-4503A (CYP3A) activity in the elderly. 
  • High level expression of mammalian protein farnesyltransferase in a baculovirus system. The purified protein contains zinc. 
  • Homeostatic mechanisms in dopamine synthesis and release: a mathematical model. 
  • Homologs of the vancomycin resistance D-Ala-D-Ala dipeptidase VanX in Streptomyces toyocaensis, Escherichia coli and Synechocystis: attributes of catalytic efficiency, stereoselectivity and regulation with implications for function. 
  • How the EcoRI endonuclease recognizes and cleaves DNA. 
  • Human IL-1 beta processing and secretion in recombinant baculovirus-infected Sf9 cells is blocked by the cowpox virus serpin crmA. 
  • Human kidney methoxyflurane and sevoflurane metabolism. Intrarenal fluoride production as a possible mechanism of methoxyflurane nephrotoxicity. 
  • Human strand-specific mismatch repair occurs by a bidirectional mechanism similar to that of the bacterial reaction. 
  • Human substance P receptor undergoes agonist-dependent phosphorylation by G protein-coupled receptor kinase 5 in vitro. 
  • Hydrolysis by somatic angiotensin-I converting enzyme of basic dipeptides from a cholecystokinin/gastrin and a LH-RH peptide extended at the C-terminus with gly-Arg/Lys-arg, but not from diarginyl insulin. 
  • IP7 guards the CDK gate. 
  • Identification and characterization of the enzymatic activity of zeta-crystallin from guinea pig lens. A novel NADPH:quinone oxidoreductase. 
  • Identification and inhibitory properties of a novel Ca(2+)/calmodulin antagonist. 
  • Identification of 3-hydroxy-2-(3-hydroxyphenyl)-4H-1-benzopyran-4-ones as isoform-selective PKC-zeta inhibitors and potential therapeutics for psychostimulant abuse. 
  • Identification of ENV determinants in V3 that influence the molecular anatomy of CCR5 utilization. 
  • Identification of G protein-gated and G protein-modulated ionic channels. Molecular basis for G protein action. 
  • Identification of Protease Specificity by Combining Proteome-Derived Peptide Libraries and Quantitative Proteomics. 
  • Identification of a two-component fatty acid kinase responsible for host fatty acid incorporation by Staphylococcus aureus. 
  • Identification of an ADAMTS-4 cleavage motif using phage display leads to the development of fluorogenic peptide substrates and reveals matrilin-3 as a novel substrate. 
  • Identification of casein kinase I substrates by in vitro expression cloning screening. 
  • Identification of deacetylase substrates with the biotin switch approach. 
  • Identification of key amino acids responsible for the substantially higher affinities of human type 1 3beta-hydroxysteroid dehydrogenase/isomerase (3beta-HSD1) for substrates, coenzymes, and inhibitors relative to human 3beta-HSD2. 
  • Identification of mitochondrial cytochrome P450 induced in response to polycyclic aromatic hydrocarbons in the mummichog (Fundulus heteroclitus). 
  • Identification of substrate specificity determinants for the cell cycle-regulated NIMA protein kinase. 
  • Identification of undecaprenyl phosphate-beta-D-galactosamine in Francisella novicida and its function in lipid A modification. 
  • Identification, partial purification, and characterization of a novel phospholipid-dependent and fatty acid-activated protein kinase from human platelets. 
  • Imaging of musculoskeletal bacterial infections by [124I]FIAU-PET/CT. 
  • Improved speciation of dissolved organic nitrogen in natural waters: amide hydrolysis with fluorescence derivatization. 
  • Improving upon nature: active site remodeling produces highly efficient aldolase activity toward hydrophobic electrophilic substrates. 
  • In vitro studies on the bacteriophage P2 terminase system. 
  • Influence of CYP3A5 genotype on the pharmacokinetics and pharmacodynamics of the cytochrome P4503A probes alfentanil and midazolam. 
  • Influence of metal ions on substrate binding and catalytic activity of mammalian protein geranylgeranyltransferase type-I. 
  • Inhibition of DNA helicase II unwinding and ATPase activities by DNA-interacting ligands. Kinetics and specificity. 
  • Inhibition of polysome assembly enhances imatinib activity against chronic myelogenous leukemia and overcomes imatinib resistance. 
  • Initiating cellular stress responses. 
  • Initiation of methyl-directed mismatch repair. 
  • Integrating energy calculations with functional assays to decipher the specificity of G protein-RGS protein interactions. 
  • Interaction of nephrocystin-4 and RPGRIP1 is disrupted by nephronophthisis or Leber congenital amaurosis-associated mutations. 
  • Interaction of prenylcysteine methyl esters with the multidrug resistance transporter. 
  • Interplay of catalysis, fidelity, threading, and processivity in the exo- and endonucleolytic reactions of human exonuclease I. 
  • Intrinsic disorder drives N-terminal ubiquitination by Ube2w. 
  • Investigating the Role of Class I Adenylate-Forming Enzymes in Natural Product Biosynthesis. 
  • Investigation of the conserved reentrant membrane helix in the monotopic phosphoglycosyl transferase superfamily supports key molecular interactions with polyprenol phosphate substrates. 
  • Involvement of outside DNA sequences in the major kinetic path by which EcoRI endonuclease locates and leaves its recognition sequence. 
  • Isolation and characterization of a dual-substrate phosphodiesterase gene family: PDE10A. 
  • Isolation and characterization of a prenylcysteine lyase from bovine brain. 
  • Isolation and characterization of the CYP71D16 trichome-specific promoter from Nicotiana tabacum L. 
  • Isolation and characterization of the Escherichia coli mutH gene product. 
  • Isolation of a cDNA clone complementary to sequences for a 34-kilodalton protein which is a pp60v-src substrate. 
  • Isolation of a nuclease-resistant decoy RNA that can protect human acetylcholine receptors from myasthenic antibodies. 
  • Isolation of protein prenyltransferases from bovine brain and baculovirus expression system. 
  • JAZ repressor proteins are targets of the SCF(COI1) complex during jasmonate signalling. 
  • Kdo hydroxylase is an inner core assembly enzyme in the Ko-containing lipopolysaccharide biosynthesis. 
  • Kinetic analysis of the effects of glycosaminoglycans and lipoproteins on urokinase-mediated plasminogen activation. 
  • Kinetic analysis of the zinc-dependent deacetylase in the lipid A biosynthetic pathway. 
  • Kinetic characterization of Salmonella FliK-FlhB interactions demonstrates complexity of the Type III secretion substrate-specificity switch. 
  • Kinetic mechanism of adenine phosphoribosyltransferase from Leishmania donovani. 
  • Kinetic mechanism of quinone oxidoreductase 2 and its inhibition by the antimalarial quinolines. 
  • Kinetic scheme for intermolecular RNA cleavage by a ribozyme derived from hepatitis delta virus RNA. 
  • Kinetic studies of protein farnesyltransferase mutants establish active substrate conformation. 
  • Kinetic study of prolidase activity in erythrocytes against different substrates using capillary electrophoresis with electrochemiluminescence detection. 
  • Kinetics and physiologic relevance of the inactivation of alpha 1-proteinase inhibitor, alpha 1-antichymotrypsin, and antithrombin III by matrix metalloproteinases-1 (tissue collagenase), -2 (72-kDa gelatinase/type IV collagenase), and -3 (stromelysin). 
  • Knock-down of LAR protein tyrosine phosphatase induces insulin resistance. 
  • LAT: the ZAP-70 tyrosine kinase substrate that links T cell receptor to cellular activation. 
  • Late onset N-acetylglutamate synthase deficiency caused by hypomorphic alleles. 
  • Lic4, a nuclear phosphoprotein that cooperates with calcineurin to regulate cation homeostasis in Saccharomyces cerevisiae. 
  • Lipoprotein (a): purification and kinetic analysis. 
  • Lysine(164)alpha of protein farnesyltransferase is important for both CaaX substrate binding and catalysis. 
  • Lysine-Specific Demethylase 1A (KDM1A/LSD1): Product Recognition and Kinetic Analysis of Full-Length Histones. 
  • Mammalian chymotrypsin-like enzymes. Comparative reactivities of rat mast cell proteases, human and dog skin chymases, and human cathepsin G with peptide 4-nitroanilide substrates and with peptide chloromethyl ketone and sulfonyl fluoride inhibitors. 
  • Mathematical analysis of the regulation of competing methyltransferases. 
  • Measurement of 25-hydroxyvitamin D-1 alpha-hydroxylase activity in mammalian kidney. 
  • Mechanism of specific site location and DNA cleavage by EcoR I endonuclease. 
  • Mechanism of the Class I KDPG aldolase. 
  • Mechanism of time-dependent inhibition of 5 alpha-reductases by delta 1-4-azasteroids: toward perfection of rates of time-dependent inhibition by using ligand-binding energies. 
  • Mechanism-Based Inhibitors of the Human Sirtuin 5 Deacylase: Structure-Activity Relationship, Biostructural, and Kinetic Insight. 
  • Members of the G protein-coupled receptor kinase family that phosphorylate the beta2-adrenergic receptor facilitate sequestration. 
  • Membrane localization of the kinase which phosphorylates p34cdc2 on threonine 14. 
  • Membrane translocation and regulation of uridine diphosphate-glucuronic acid uptake in rat liver microsomal vesicles. 
  • Metabolism of γ-hydroxybutyrate in perfused rat livers. 
  • Metformin Targets Central Carbon Metabolism and Reveals Mitochondrial Requirements in Human Cancers. 
  • Methotrexate recognition by the human reduced folate carrier SLC19A1. 
  • Microtubules accelerate the kinase activity of Aurora-B by a reduction in dimensionality. 
  • Minimal PAM specificity of a highly similar SpCas9 ortholog. 
  • Mitochondrial metabolism sets the maximal limit of fuel-stimulated insulin secretion in a model pancreatic beta cell: a survey of four fuel secretagogues. 
  • Molecular and biochemical characterization of two plant inositol polyphosphate 6-/3-/5-kinases. 
  • Molecular characterization of CYP2B6 substrates. 
  • Molecular cloning and functional expression of a recombinant 72.5 kDa fragment of the 110 kDa regulatory subunit of smooth muscle protein phosphatase 1M. 
  • Molecular studies of human renin structure and synthesis using monoclonal antibodies. 
  • Monoamine oxidase of rat skeletal muscle: substrate specificity and half-life. 
  • Monoclonal antibody blockade of IL-2 receptor α during lymphopenia selectively depletes regulatory T cells in mice and humans. 
  • Multifunctional reaper: sixty-five amino acids of fury. 
  • Multiple domains of MCIP1 contribute to inhibition of calcineurin activity. 
  • Multiple forms of rat liver type I topoisomerase. 
  • MutS and MutL activate DNA helicase II in a mismatch-dependent manner. 
  • Mutagenesis of the phosphate-binding pocket of KDPG aldolase enhances selectivity for hydrophobic substrates. 
  • Mutagenesis studies of substrate recognition and catalysis in the sortase A transpeptidase from Staphylococcus aureus. 
  • Mutants of the EcoRI endonuclease with promiscuous substrate specificity implicate residues involved in substrate recognition. 
  • Mutation detection with MutH, MutL, and MutS mismatch repair proteins. 
  • Mutational analysis of a type II topoisomerase cleavage site: distinct requirements for enzyme and inhibitors. 
  • Mutational analysis of the function of Met137 and Ile197, two amino acids implicated in sequence-specific DNA recognition by the EcoRI endonuclease. 
  • N7-cyanoborane-2'-deoxyguanosine 5'-triphosphate is a good substrate for DNA polymerase. 
  • NMR-based Structural Analysis of Threonylcarbamoyl-AMP Synthase and Its Substrate Interactions. 
  • Nitric oxide-associated regulation of hepatocyte glutathione synthesis is a guanylyl cyclase-independent event. 
  • Noncatalytic domains of RGS9-1.Gbeta 5L play a decisive role in establishing its substrate specificity. 
  • Not-so-pseudo a substrate: Acm1-mediated inhibition of the APC. 
  • Nuclear import/export of hRPF1/Nedd4 regulates the ubiquitin-dependent degradation of its nuclear substrates. 
  • Nucleotide bias of DCL and AGO in plant anti-virus gene silencing. 
  • Nucleotide regulation of soluble guanylate cyclase substrate specificity. 
  • Nucleotide sequence preference at rat liver and wheat germ type 1 DNA topoisomerase breakage sites in duplex SV40 DNA. 
  • Nucleotide-promoted release of hMutSalpha from heteroduplex DNA is consistent with an ATP-dependent translocation mechanism. 
  • Okadaic acid mimics the action of insulin in stimulating protein kinase activity in isolated adipocytes. The role of protein phosphatase 2a in attenuation of the signal. 
  • One binding site determines sequence specificity of Tetrahymena pre-rRNA self-splicing, trans-splicing, and RNA enzyme activity. 
  • One-carbon metabolism and epigenetics: understanding the specificity. 
  • Optimization of a Novel Series of Ataxia-Telangiectasia Mutated Kinase Inhibitors as Potential Radiosensitizing Agents. 
  • Optimizing the substrate specificity of a group I intron ribozyme. 
  • Ordered phosphorylation of p42mapk by MAP kinase kinase. 
  • Overexpression of protein targeting to glycogen (PTG) in rat hepatocytes causes profound activation of glycogen synthesis independent of normal hormone- and substrate-mediated regulatory mechanisms. 
  • PITK, a PP1 targeting subunit that modulates the phosphorylation of the transcriptional regulator hnRNP K. 
  • Peptide specificity determinants at P-7 and P-6 enhance the catalytic efficiency of Ca2+/calmodulin-dependent protein kinase I in the absence of activation loop phosphorylation. 
  • Phosphorylation and desensitization of human endothelin A and B receptors. Evidence for G protein-coupled receptor kinase specificity. 
  • Phosphorylation of the cystic fibrosis transmembrane conductance regulator. 
  • Phosphorylation of tristetraprolin, a potential zinc finger transcription factor, by mitogen stimulation in intact cells and by mitogen-activated protein kinase in vitro. 
  • Phosphorylation specificities of protein kinase C isozymes for bovine cardiac troponin I and troponin T and sites within these proteins and regulation of myofilament properties. 
  • Platelet-activating factor induces tyrosine phosphorylation in human neutrophils. 
  • Polyglutamine domains are substrates of tissue transglutaminase: does transglutaminase play a role in expanded CAG/poly-Q neurodegenerative diseases? 
  • Potent anticoagulant aptamer directed against factor IXa blocks macromolecular substrate interaction. 
  • Preferential inhibition of Akt and killing of Akt-dependent cancer cells by rationally designed phosphatidylinositol ether lipid analogues. 
  • Presenilin-dependent gamma-secretase-like intramembrane cleavage of ErbB4. 
  • Probing the catalytic mechanism of sulfite reductase by X-ray crystallography: structures of the Escherichia coli hemoprotein in complex with substrates, inhibitors, intermediates, and products. 
  • Processive post-translational modification. Vitamin K-dependent carboxylation of a peptide substrate. 
  • Profiling microRNA expression in hepatocellular carcinoma reveals microRNA-224 up-regulation and apoptosis inhibitor-5 as a microRNA-224-specific target. 
  • Properties and kinetic mechanism of recombinant mammalian protein geranylgeranyltransferase type I. 
  • Properties and regulation of the cell cycle-specific NIMA protein kinase of Aspergillus nidulans. 
  • Protein (serine and threonine) phosphate phosphatases. 
  • Protein Serine/Threonine Phosphatases: Keys to Unlocking Regulators and Substrates. 
  • Protein farnesyltransferase and geranylgeranyltransferase share a common alpha subunit. 
  • Protein farnesyltransferase. 
  • Protein kinase C beta II specifically binds to and is activated by F-actin. 
  • Protein kinase C mediates cholinergically regulated protein phosphorylation in a Cl(-)-secreting epithelium. 
  • Protein kinases that phosphorylate activated G protein-coupled receptors. 
  • Protein phosphatase 1 regulation by inhibitors and targeting subunits. 
  • Protein phosphatase 2A inhibitors, I(1)(PP2A) and I(2)(PP2A), associate with and modify the substrate specificity of protein phosphatase 1. 
  • Protein phosphatase 2Cm is a critical regulator of branched-chain amino acid catabolism in mice and cultured cells. 
  • Protein prenyltransferases. 
  • Protein roadblocks and helix discontinuities are barriers to the initiation of mismatch repair. 
  • Proteinase activity and stability of natural bromelain preparations. 
  • Proteomic analysis of calcium/calmodulin-dependent protein kinase I and IV in vitro substrates reveals distinct catalytic preferences. 
  • Proteomic identification and functional characterization of MYH9, Hsc70, and DNAJA1 as novel substrates of HDAC6 deacetylase activity. 
  • Pure MnTBAP selectively scavenges peroxynitrite over superoxide: comparison of pure and commercial MnTBAP samples to MnTE-2-PyP in two models of oxidative stress injury, an SOD-specific Escherichia coli model and carrageenan-induced pleurisy. 
  • Purification and characterization of the mammalian myosin light chain phosphatase holoenzyme. The differential effects of the holoenzyme and its subunits on smooth muscle. 
  • Purification and identification of two serine class proteinases from dog mast biochemically and immunologically similar to human proteinases tryptase and chymase. 
  • Purification and mutagenesis of LpxL, the lauroyltransferase of Escherichia coli lipid A biosynthesis. 
  • Purification and partial characterization of a thiol proteinase from the thermophilic fungus Humicola lanuginosa. 
  • Purification and properties of EcoRI endonuclease. 
  • Purification and properties of a multifunctional calcium/calmodulin-dependent protein kinase from rat pancreas. 
  • Purification of an almond emulsin fucosidase on Cibacron blue-sepharose and demonstration of its activity toward fucose-containing glycoproteins. 
  • Quantification of DNA cleavage specificity in Hi-C experiments. 
  • Quantifying intrinsic specificity: a potential complement to affinity in drug screening. 
  • Quantitative distinctions of active site molecular recognition by P-glycoprotein and cytochrome P450 3A4. 
  • R73A and H144Q mutants of the yeast mitochondrial cyclophilin Cpr3 exhibit a low prolyl isomerase activity in both peptide and protein-folding assays. 
  • RGS9-G beta 5 substrate selectivity in photoreceptors. Opposing effects of constituent domains yield high affinity of RGS interaction with the G protein-effector complex. 
  • RNase H activation by stereoregular boranophosphate oligonucleotide. 
  • Rapid analysis of protein farnesyltransferase substrate specificity using peptide libraries and isoprenoid diphosphate analogues. 
  • Rapid diagnosis of late-onset Pompe disease by fluorometric assay of alpha-glucosidase activities in dried blood spots. 
  • Rapid optimization of enzyme substrates using defined substrate mixtures. 
  • Re-design of Rhodobacter sphaeroides dimethyl sulfoxide reductase. Enhancement of adenosine N1-oxide reductase activity. 
  • Reaction mechanism of (6-4) photolyase. 
  • Reaction path of protein farnesyltransferase at atomic resolution. 
  • Reactive Acyl-CoA Species Modify Proteins and Induce Carbon Stress. 
  • Receptor-specific desensitization with purified proteins. Kinase dependence and receptor specificity of beta-arrestin and arrestin in the beta 2-adrenergic receptor and rhodopsin systems. 
  • Reciprocal regulation of the platelet-derived growth factor receptor-beta and G protein-coupled receptor kinase 5 by cross-phosphorylation: effects on catalysis. 
  • Recognition of single-stranded DNA by the bacteriophage T4-induced type II topoisomerase. 
  • Recognition sequence of the dam methylase of Escherichia coli K12 and mode of cleavage of Dpn I endonuclease. 
  • Recombinant Rhodobacter capsulatus xanthine dehydrogenase, a useful model system for the characterization of protein variants leading to xanthinuria I in humans. 
  • Reconstitution of mitochondrial processing peptidase from the core proteins (subunits I and II) of bovine heart mitochondrial cytochrome bc(1) complex. 
  • Recruiting substrates to cullin 4-dependent ubiquitin ligases by DDB1. 
  • Refined solution structure of the LpxC-TU-514 complex and pKa analysis of an active site histidine: insights into the mechanism and inhibitor design. 
  • Regulation of G protein-coupled receptor kinase 5 (GRK5) by actin. 
  • Regulation of G protein-coupled receptor kinases and arrestins during receptor desensitization. 
  • Regulation of phosphatidate phosphatase activity from the yeast Saccharomyces cerevisiae by sphingoid bases. 
  • Regulation of protein kinases by pseudosubstrate prototopes. 
  • Regulation of rat heart ornithine decarboxylase: change in affinity for ornithine evoked by neuronal, hormonal, and ontogenetic stimuli. 
  • Regulatory cascades involving calmodulin-dependent protein kinases. 
  • Removal of the pro-domain does not affect the conformation of the procaspase-3 dimer. 
  • Restricted substrate specificity for the geranylgeranyltransferase-I enzyme in Cryptococcus neoformans: implications for virulence. 
  • Retargeting mobile group II introns to repair mutant genes. 
  • Role of acidic amino acids in peptide substrates of the beta-adrenergic receptor kinase and rhodopsin kinase. 
  • Rotavirus RNA polymerase requires the core shell protein to synthesize the double-stranded RNA genome. 
  • SENP3-mediated host defense response contains HBV replication and restores protein synthesis. 
  • SP75 is encoded by the DP87 gene and belongs to a family of modular Dictyostelium discoideum outer layer spore coat proteins. 
  • Saccharomyces cerevisiae Ntg1p and Ntg2p: broad specificity N-glycosylases for the repair of oxidative DNA damage in the nucleus and mitochondria. 
  • Saturation mutagenesis of His114 of EcoRI reveals relaxed-specificity mutants. 
  • Selection of peptides that target the aminoacyl-tRNA site of bacterial 16S ribosomal RNA. 
  • Site-specific incorporation of 3-nitrotyrosine as a probe of pKa perturbation of redox-active tyrosines in ribonucleotide reductase. 
  • Site-specific mutagenesis of Drosophila alcohol dehydrogenase: evidence for involvement of tyrosine-152 and lysine-156 in catalysis. 
  • Site-specific retinoic acid production in the brain of adult songbirds. 
  • Small-Molecule Positive Allosteric Modulators of the β2-Adrenoceptor Isolated from DNA-Encoded Libraries. 
  • Smooth muscle myosin phosphatase-associated kinase induces Ca2+ sensitization via myosin phosphatase inhibition. 
  • Sodium binding site of factor Xa: role of sodium in the prothrombinase complex. 
  • Sortase transpeptidases: insights into mechanism, substrate specificity, and inhibition. 
  • Specificity determinants in phosphoinositide dephosphorylation: crystal structure of an archetypal inositol polyphosphate 5-phosphatase. 
  • Specificity of the type II secretion systems of enterotoxigenic Escherichia coli and Vibrio cholerae for heat-labile enterotoxin and cholera toxin. 
  • Stereoselective Bupropion Hydroxylation by Cytochrome P450 CYP2B6 and Cytochrome P450 Oxidoreductase Genetic Variants. 
  • Stereospecificity and kinetic mechanism of human prenylcysteine lyase, an unusual thioether oxidase. 
  • Structural adaptations that modulate monosaccharide, disaccharide, and trisaccharide specificities in periplasmic maltose-binding proteins. 
  • Structural analysis and detection of biological inositol pyrophosphates reveal that the family of VIP/diphosphoinositol pentakisphosphate kinases are 1/3-kinases. 
  • Structural and Functional Characterization of Sulfonium Carbon-Oxygen Hydrogen Bonding in the Deoxyamino Sugar Methyltransferase TylM1. 
  • Structural basis for binding and selectivity of antimalarial and anticancer ethylenediamine inhibitors to protein farnesyltransferase. 
  • Structural basis for impaired 5' processing of a mutant tRNA associated with defects in neuronal homeostasis. 
  • Structural basis for the recognition of a bisphosphorylated MAP kinase peptide by human VHR protein Phosphatase. 
  • Structural basis of beta-adrenergic receptor subtype specificity studied with chimeric beta 1/beta 2-adrenergic receptors. 
  • Structural basis of core promoter recognition in a primitive eukaryote. 
  • Structural basis of nucleoside and nucleoside drug selectivity by concentrative nucleoside transporters. 
  • Structural basis of odorant recognition by a human odorant receptor. 
  • Structural domains that contribute to substrate specificity in facilitated glucose transporters are distinct from those involved in kinetic function: studies with GLUT-1/GLUT-2 chimeras. 
  • Structural factors that determine selectivity of a high fidelity DNA polymerase for deoxy-, dideoxy-, and ribonucleotides. 
  • Structural mechanism of organic hydroperoxide induction of the transcription regulator OhrR. 
  • Structural studies and protein engineering of inositol phosphate multikinase. 
  • Structure of mammalian protein geranylgeranyltransferase type-I. 
  • Structure of protein geranylgeranyltransferase-I from the human pathogen Candida albicans complexed with a lipid substrate. 
  • Structure of the LpxC deacetylase with a bound substrate-analog inhibitor. 
  • Structure of the N-terminal domain of GRP94. Basis for ligand specificity and regulation. 
  • Structure of the deacetylase LpxC bound to the antibiotic CHIR-090: Time-dependent inhibition and specificity in ligand binding. 
  • Structure of the essential Haemophilus influenzae UDP-diacylglucosamine pyrophosphohydrolase LpxH in lipid A biosynthesis. 
  • Structures and mechanisms of DNA restriction and modification enzymes. 
  • Structures of BmrR-drug complexes reveal a rigid multidrug binding pocket and transcription activation through tyrosine expulsion. 
  • Structures of Cryptococcus neoformans protein farnesyltransferase reveal strategies for developing inhibitors that target fungal pathogens. 
  • Structures of the pleiotropic translational regulator Hfq and an Hfq-RNA complex: a bacterial Sm-like protein. 
  • Structures of trehalose-6-phosphate phosphatase from pathogenic fungi reveal the mechanisms of substrate recognition and catalysis. 
  • Studies on sequence recognition by type II restriction and modification enzymes. 
  • Studies on the regulation and specificity of the DNA-untwisting enzyme. 
  • Substitution of cadmium for zinc in farnesyl:protein transferase alters its substrate specificity. 
  • Substrate Selectivity of Lysophospholipid Transporter LplT Involved in Membrane Phospholipid Remodeling in Escherichia coli. 
  • Substrate dependence of the mechanism of EcoRI endonuclease. 
  • Substrate promiscuity: AglB, the archaeal oligosaccharyltransferase, can process a variety of lipid-linked glycans. 
  • Substrate recognition by the EcoRI endonuclease. 
  • Substrate specificities and identification of putative substrates of ATM kinase family members. 
  • Substrate specificities of g protein-coupled receptor kinase-2 and -3 at cardiac myocyte receptors provide basis for distinct roles in regulation of myocardial function. 
  • Substrate specificity of Ca2+/CaM-dependent multifunctional protein kinases: comparison of isoenzymes from brain, liver and skeletal muscle. 
  • Substrate spectrum of human excinuclease: repair of abasic sites, methylated bases, mismatches, and bulky adducts. 
  • Substrate-dependent activation energy of the reaction catalyzed by monoamine oxidase. 
  • Suicide substrates for the alanine racemase of Escherichia coli B. 
  • Synthesis of P(1)-Citronellyl-P(2)-alpha-D-pyranosyl pyrophosphates as potential substrates for the E. coli undecaprenyl-pyrophosphoryl-N-acetylglucoseaminyl transferase MurG. 
  • Synthesis of acyclothymidine triphosphate and alpha-P-boranotriphosphate and their substrate properties with retroviral reverse transcriptase. 
  • Synthesis of programmable integrases. 
  • Synthetic HIV V3 Glycopeptide Immunogen Carrying a N334 N-Glycan Induces Glycan-Dependent Antibodies with Promiscuous Site Recognition. 
  • Synthetic peptides of the hamster beta 2-adrenoceptor as substrates and inhibitors of the beta-adrenoceptor kinase. 
  • T-antigen kinase inhibits simian virus 40 DNA replication by phosphorylation of intact T antigen on serines 120 and 123. 
  • Tagging ribozyme reaction sites to follow trans-splicing in mammalian cells. 
  • Targeted disruption of mouse long-chain acyl-CoA dehydrogenase gene reveals crucial roles for fatty acid oxidation. 
  • Targeted gene replacement demonstrates that myristoyl-CoA: protein N-myristoyltransferase is essential for viability of Cryptococcus neoformans. 
  • Targeting the PI3K/Akt/mTOR pathway: effective combinations and clinical considerations. 
  • Tetanus toxoid and CCL3 improve dendritic cell vaccines in mice and glioblastoma patients. 
  • The 6.9-A structure of GlpF: a basis for homology modeling of the glycerol channel from Escherichia coli. 
  • The ATM-dependent DNA damage signaling pathway. 
  • The Abl family kinases: mechanisms of regulation and signaling. 
  • The DEXH protein product of the DHX36 gene is the major source of tetramolecular quadruplex G4-DNA resolving activity in HeLa cell lysates. 
  • The DNA helicase activities of Rad3 protein of Saccharomyces cerevisiae and helicase II of Escherichia coli are differentially inhibited by covalent and noncovalent DNA modifications. 
  • The G-protein-coupled receptor phosphatase: a protein phosphatase type 2A with a distinct subcellular distribution and substrate specificity. 
  • The LS locus of pea encodes the gibberellin biosynthesis enzyme ent-kaurene synthase A. 
  • The RNA polymerase II CTD kinase CTDK-I affects pre-mRNA 3' cleavage/polyadenylation through the processing component Pti1p. 
  • The Tetrahymena ribozyme acts like an RNA restriction endonuclease. 
  • The acceptor substrate specificity of porcine submaxillary UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase is dependent on the amino acid sequences adjacent to serine and threonine residues. 
  • The basis for K-Ras4B binding specificity to protein farnesyltransferase revealed by 2 A resolution ternary complex structures. 
  • The biological significance of substrate inhibition: a mechanism with diverse functions. 
  • The c-Abl tyrosine kinase is regulated downstream of the B cell antigen receptor and interacts with CD19. 
  • The catalytic region and PEST domain of PTPN18 distinctly regulate the HER2 phosphorylation and ubiquitination barcodes. 
  • The distinct binding specificities exhibited by enterobacterial type 1 fimbriae are determined by their fimbrial shafts. 
  • The evaluation of complex-dependent alterations in human factor VIIa. 
  • The influence of the synergistic anion on iron chelation by ferric binding protein, a bacterial transferrin. 
  • The mammalian target of rapamycin phosphorylates sites having a (Ser/Thr)-Pro motif and is activated by antibodies to a region near its COOH terminus. 
  • The mechanism of the translocation step in DNA replication by DNA polymerase I: a computer simulation analysis. 
  • The other function of DNA photolyase: stimulation of excision repair of chemical damage to DNA. 
  • The p16 status of tumor cell lines identifies small molecule inhibitors specific for cyclin-dependent kinase 4. 
  • The protein backbone makes important contributions to 4-oxalocrotonate tautomerase enzyme catalysis: understanding from theory and experiment. 
  • The structural basis for the selectivity of benzotriazole inhibitors of PTP1B. 
  • The structural mechanism of GTP stabilized oligomerization and catalytic activation of the Toxoplasma gondii uracil phosphoribosyltransferase. 
  • The structure of helical lipoprotein lipase reveals an unexpected twist in lipase storage. 
  • The substrate specificity of cyclic AMP-dependent protein kinase: amino acid sequences at the phosphorylation sites of herring protamine (clupeine). 
  • The use of immobilized osteogenic growth peptide on gradient substrates synthesized via click chemistry to enhance MC3T3-E1 osteoblast proliferation. 
  • Thematic review series: lipid posttranslational modifications. Structural biology of protein farnesyltransferase and geranylgeranyltransferase type I. 
  • Theoretical and experimental determination on two substrates turned over by 4-oxalocrotonate tautomerase. 
  • Thermodynamic analysis of a molecular chaperone binding to unfolded protein substrates. 
  • Thermodynamic characterization of the binding interaction between the histone demethylase LSD1/KDM1 and CoREST. 
  • Thrombin-catalyzed activation of recombinant human factor V. 
  • Toward a Molecular Basis of Cellular Nucleoside Transport in Humans. 
  • Trans-splicing reactions by ribozymes. 
  • Transcription Factor-DNA Binding Motifs in Saccharomyces cerevisiae: Tools and Resources. 
  • Transcriptional network of multiple capsule and melanin genes governed by the Cryptococcus neoformans cyclic AMP cascade. 
  • Transglutaminase-catalyzed inactivation of glyceraldehyde 3-phosphate dehydrogenase and alpha-ketoglutarate dehydrogenase complex by polyglutamine domains of pathological length. 
  • Truncated forms of the insulin-like growth factor II (IGF-II)/mannose 6-phosphate receptor encompassing the IGF-II binding site: characterization of a point mutation that abolishes IGF-II binding. 
  • Tunable leuko-polymersomes that adhere specifically to inflammatory markers. 
  • Two conserved domains in regulatory B subunits mediate binding to the A subunit of protein phosphatase 2A. 
  • Two glutathione peroxidases in the fungal pathogen Cryptococcus neoformans are expressed in the presence of specific substrates. 
  • Understanding specificity and sensitivity of T-cell recognition. 
  • Use of a multiple-enzyme/multiple-reagent assay system to quantify activity levels in samples containing mixtures of matrix metalloproteinases. 
  • Use of pH and kinetic isotope effects to establish chemistry as rate-limiting in oxidation of a peptide substrate by LSD1. 
  • Vascular regulation of plasminogen activator inhibitor-1 activity. 
  • YbiV from Escherichia coli K12 is a HAD phosphatase. 
  • [Localization of binding sites of E. coli DNA-dependent RNA-polymerase with photosensitive template analogs]. 
  • alpha-Actinin is a potent regulator of G protein-coupled receptor kinase activity and substrate specificity in vitro. 
  • cDNA cloning and expression of rat and human protein geranylgeranyltransferase type-I. 
  • iPABP, an inducible poly(A)-binding protein detected in activated human T cells. 
  • rac, a novel ras-related family of proteins that are botulinum toxin substrates. 
• 

  Keywords of People

  • Bennett, Vann, George Barth Geller Distinguished Professor of Molecular Biology, Duke Cancer Institute
  • Ferreira, Paulo Alexandre, Associate Professor in Ophthalmology, Pathology
  • Sampson, John Howard, Robert H., M.D. and Gloria Wilkins Professor of Neurosurgery, in the School of Medicine, Biomedical Engineering
  • Soderling, Scott Haydn, George Barth Geller Distinguished Professor of Molecular Biology, Cell Biology
  • Sullivan, Daniel Carl, Professor Emeritus of Radiology, Radiology
  • Weinhold, Kent James, Joseph W. and Dorothy W. Beard Distinguished Professor of Experimental Surgery, Integrative Immunobiology