Tetrahymena

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  Subject Areas on Research

  • Circularizing ribozymes and decoy-competitors by autocatalytic splicing in vitro and in vivo. 
  • Evaluating group I intron catalytic efficiency in mammalian cells. 
  • Functional repair of a mutant chloride channel using a trans-splicing ribozyme. 
  • Group I intron self-splicing with adenosine: evidence for a single nucleoside-binding site. 
  • Group I permuted intron-exon (PIE) sequences self-splice to produce circular exons. 
  • One binding site determines sequence specificity of Tetrahymena pre-rRNA self-splicing, trans-splicing, and RNA enzyme activity. 
  • Probing the interplay between the two steps of group I intron splicing: competition of exogenous guanosine with omega G. 
  • RNA Structural Modules Control the Rate and Pathway of RNA Folding and Assembly. 
  • RNA as an RNA polymerase: net elongation of an RNA primer catalyzed by the Tetrahymena ribozyme. 
  • Selection of circularization sites in a group I IVS RNA requires multiple alignments of an internal template-like sequence. 
  • Sites of circularization of the Tetrahymena rRNA IVS are determined by sequence and influenced by position and secondary structure. 
  • Structures involved in Tetrahymena rRNA self-splicing and RNA enzyme activity. 
  • The Tetrahymena ribozyme acts like an RNA restriction endonuclease. 
  • Visualizing the formation of an RNA folding intermediate through a fast highly modular secondary structure switch. 
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  Keywords of People

  • Been, Michael Douglas, Professor Emeritus of Biochemistry, Biochemistry