Transformation, Genetic

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  Subject Areas on Research

  • A Caenorhabditis elegans act-4::lacZ fusion: use as a transformation marker and analysis of tissue-specific expression. 
  • A bifunctional dihydrofolate synthetase--folylpolyglutamate synthetase in Plasmodium falciparum identified by functional complementation in yeast and bacteria. 
  • A human anti-HIV autoantibody enhances EBV transformation and HIV infection. 
  • A requirement for NF-kappaB activation in Bcr-Abl-mediated transformation. 
  • A system for gene cloning and manipulation in the yeast Candida glabrata. 
  • A yeast expression system for human galactose-1-phosphate uridylyltransferase. 
  • Adenine auxotrophic mutants of Aspergillus oryzae: development of a novel transformation system with triple auxotrophic hosts. 
  • Advancing Functional Genetics Through Agrobacterium-Mediated Insertional Mutagenesis and CRISPR/Cas9 in the Commensal and Pathogenic Yeast Malassezia. 
  • Amino acid changes in Xrs2p, Dun1p, and Rfa2p that remove the preferred targets of the ATM family of protein kinases do not affect DNA repair or telomere length in Saccharomyces cerevisiae. 
  • An examination of adaptive reversion in Saccharomyces cerevisiae. 
  • Antifolate resistance due to new and known Plasmodium falciparum dihydrofolate reductase mutations expressed in yeast. 
  • Antisense repression in Cryptococcus neoformans as a laboratory tool and potential antifungal strategy. 
  • Association of increased spontaneous mutation rates with high levels of transcription in yeast. 
  • BIN1 is a novel MYC-interacting protein with features of a tumour suppressor. 
  • Biolistic nuclear transformation of Saccharomyces cerevisiae and other fungi. 
  • CNS1 encodes an essential p60/Sti1 homolog in Saccharomyces cerevisiae that suppresses cyclophilin 40 mutations and interacts with Hsp90. 
  • Characterization of transformed Arabidopsis with altered alternative oxidase levels and analysis of effects on reactive oxygen species in tissue. 
  • Chromosomal organization of rRNA operons in Bacillus subtilis. 
  • Circular polymerase extension cloning for high-throughput cloning of complex and combinatorial DNA libraries. 
  • Circular polymerase extension cloning. 
  • Cloning and expression of the yeast plasma membrane ATPase in Escherichia coli. 
  • Combinatorial and synergistic properties of CaMV 35S enhancer subdomains. 
  • Comparison of in vitro activities of camptothecin and nitidine derivatives against fungal and cancer cells. 
  • Conditional dicentric chromosomes in yeast. 
  • Contribution of chromosomal polymorphisms to the G-matrix of Mimulus guttatus. 
  • Cryptococcus neoformans differential gene expression detected in vitro and in vivo with green fluorescent protein. 
  • Development of a novel quadruple auxotrophic host transformation system by argB gene disruption using adeA gene and exploiting adenine auxotrophy in Aspergillus oryzae. 
  • Diploid strains of the pathogenic basidiomycete Cryptococcus neoformans are thermally dimorphic. 
  • Double-strand break repair in tandem repeats during bacteriophage T4 infection. 
  • Effects of nitric oxide on the copper-responsive transcription factor Ace1 in Saccharomyces cerevisiae: cytotoxic and cytoprotective actions of nitric oxide. 
  • Efficient transposition of the piggyBac (PB) transposon in mammalian cells and mice. 
  • Evaluation of a recombinant yeast cell estrogen screening assay. 
  • Evolutionary genetics: gene replacement and the genetics of speciation. 
  • Experimental determination of rates of concerted evolution. 
  • Expression of cDNAs for G proteins in Escherichia coli. Two forms of Gs alpha stimulate adenylate cyclase. 
  • Expression of spinach glycolate oxidase in Saccharomyces cerevisiae: purification and characterization. 
  • Functional analysis of the TAN-1 gene, a human homolog of Drosophila notch. 
  • Gene Function Analysis in the Ubiquitous Human Commensal and Pathogen Malassezia Genus. 
  • Gene disruption by biolistic transformation in serotype D strains of Cryptococcus neoformans. 
  • Generation of mucosal anti-human immunodeficiency virus type 1 T-cell responses by recombinant Mycobacterium smegmatis. 
  • Genetic mapping of Ty elements in Saccharomyces cerevisiae. 
  • Hmo1p, a high mobility group 1/2 homolog, genetically and physically interacts with the yeast FKBP12 prolyl isomerase. 
  • Identification of a Cryptococcus neoformans gene that directs expression of the cryptic Saccharomyces cerevisiae mannitol dehydrogenase gene. 
  • Identification of a second family of high-molecular-weight adhesion proteins expressed by non-typable Haemophilus influenzae. 
  • Impact of ammonium permeases mepA, mepB, and mepC on nitrogen-regulated secondary metabolism in Fusarium fujikuroi. 
  • Influence of amino acids encoded in the 3' open reading frame of the SV40 early region on transformation and antigenicity of large T antigen. 
  • Inhibition of ras-induced germinal vesicle breakdown in Xenopus oocytes by rap-1B. 
  • Inhibition of retinoic acid receptor function in normal human mammary epithelial cells results in increased cellular proliferation and inhibits the formation of a polarized epithelium in vitro. 
  • Integration of DNA fragments by illegitimate recombination in Saccharomyces cerevisiae. 
  • Mammalian metallothionein is functional in yeast. 
  • Mechanisms regulating SHORT-ROOT intercellular movement. 
  • Molecular analysis of the Cryptococcus neoformans ADE2 gene, a selectable marker for transformation and gene disruption. 
  • Molecular genetics of yeast. 
  • Mutagenic effects of abasic and oxidized abasic lesions in Saccharomyces cerevisiae. 
  • Oligonucleotide transformation of yeast reveals mismatch repair complexes to be differentially active on DNA replication strands. 
  • On the evolution of genetic incompatibility systems. VI. A three-locus modifier model for the origin of gametophytic self-incompatibility. 
  • Oral gene delivery with chitosan--DNA nanoparticles generates immunologic protection in a murine model of peanut allergy. 
  • Oxidative stress survival in a clinical Saccharomyces cerevisiae isolate is influenced by a major quantitative trait nucleotide. 
  • Phospholipid-binding protein Cts1 controls septation and functions coordinately with calcineurin in Cryptococcus neoformans. 
  • Recapitulation of the sexual cycle of the primary fungal pathogen Cryptococcus neoformans var. gattii: implications for an outbreak on Vancouver Island, Canada. 
  • Reconstitution of a thermophilic Cu+ importer in vitro reveals intrinsic high-affinity slow transport driving accumulation of an essential metal ion. 
  • Regulation of P311 expression by Met-hepatocyte growth factor/scatter factor and the ubiquitin/proteasome system. 
  • Relationship between simian virus 40 large tumor antigen expression and tumor formation in transgenic mice. 
  • Retrovirally transduced human dendritic cells can generate T cells recognizing multiple MHC class I and class II epitopes from the melanoma antigen glycoprotein 100. 
  • Robust Transgene Expression from Bicistronic mRNA in the Green Alga Chlamydomonas reinhardtii. 
  • Specificity of human bactericidal antibodies against PorA P1.7,16 induced with a hexavalent meningococcal outer membrane vesicle vaccine. 
  • T lymphocytes with a normal ADA gene accumulate after transplantation of transduced autologous umbilical cord blood CD34+ cells in ADA-deficient SCID neonates. 
  • The chromosomal region containing pab-1, mip, and the A mating type locus of the secondarily homothallic homobasidiomycete Coprinus bilanatus. 
  • The claret locus in Drosophila encodes products required for eyecolor and for meiotic chromosome segregation. 
  • The gene encoding phosphoribosylaminoimidazole carboxylase (ADE2) is essential for growth of Cryptococcus neoformans in cerebrospinal fluid. 
  • The genetic basis of a flower color polymorphism in the common morning glory (Ipomoea purpurea). 
  • The suppressor gene scl1+ of Saccharomyces cerevisiae is essential for growth. 
  • Three new dominant drug resistance cassettes for gene disruption in Saccharomyces cerevisiae. 
  • Time-dependent mitotic recombination in Saccharomyces cerevisiae. 
  • Tissue-specific expression from CaMV 35S enhancer subdomains in early stages of plant development. 
  • Transformation of Saccharomyces cerevisiae with nonhomologous DNA: illegitimate integration of transforming DNA into yeast chromosomes and in vivo ligation of transforming DNA to mitochondrial DNA sequences. 
  • Transformation of rat tracheal epithelial cells to immortal growth variants by particulate and soluble nickel compounds. 
  • Unequal meiotic recombination within tandem arrays of yeast ribosomal DNA genes.