Ultracentrifugation

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  Subject Areas on Research

  • A Comparative Study of Serum Exosome Isolation Using Differential Ultracentrifugation and Three Commercial Reagents. 
  • A comparison of methods for the estimation of plasma low- and very low-density lipoprotein cholesterol. The Lipid Research Clinics Prevalence Study. 
  • Altered ultrastructural morphology of self-aggregated low density lipoproteins: coalescence of lipid domains forming droplets and vesicles. 
  • Amphitrite ornata erythrocruorin. I. Structural properties and characterization of subunit interactions. 
  • Antibody-dependent lymphoid cell-mediated cytotoxicity: role of complement. 
  • Assembly and molecular activities of the MutS tetramer. 
  • Binding of MetJ repressor to specific and nonspecific DNA and effect of S-adenosylmethionine on these interactions. 
  • Breakage of single-stranded DNA by rat liver nicking-closing enzyme with the formation of a DNA-enzyme complex. 
  • Characterization and structure of the manganese-responsive transcriptional regulator ScaR. 
  • Characterization of chemotactic activity produced in vivo by a cell-mediated immune reaction in the guinea pig. 
  • Circulating IgA immune complexes in patients with psoriasis. 
  • Computational design and elaboration of a de novo heterotetrameric alpha-helical protein that selectively binds an emissive abiological (porphinato)zinc chromophore. 
  • Concentrated RD114-pseudotyped MFGS-gp91phox vector achieves high levels of functional correction of the chronic granulomatous disease oxidase defect in NOD/SCID/beta -microglobulin-/- repopulating mobilized human peripheral blood CD34+ cells. 
  • De novo design of a single-chain diphenylporphyrin metalloprotein. 
  • Deoxyribonucleic acid ligase. Isolation and physical characterization of the homogeneous enzyme from Escherichia coli. 
  • Determination of background serum density for lipoprotein ultracentrifugation. 
  • Dissociation and reassembly of Limulus polyphemus hemocyanin. 
  • Dysregulation of proteoglycan production by intrahepatic biliary epithelial cells bearing defective (delta-f508) cystic fibrosis transmembrane conductance regulator. 
  • Effect of Mts1 on the structure and activity of nonmuscle myosin II. 
  • Extracellular matrix proteins and carcinoembryonic antigen-related cell adhesion molecules characterize pancreatic duct fluid exosomes in patients with pancreatic cancer. 
  • Factors from saliva and oral bacteria, chemotactic for polymorphonuclear leukocytes: their possible role in gingival inflammation. 
  • Functional equivalence of monomeric (shark) and dimeric (bovine) cytochrome c oxidase. 
  • Heavy endosomes isolated from the rat renal cortex show attributes of intermicrovillar clefts. 
  • Hemocyanin of the chambered nautilus: structure-function relationships. 
  • Hemoglobin Kansas, a human hemoglobin with a neutral amino acid substitution and an abnormal oxygen equilibrium. 
  • Hepatic sulfite oxidase. Identification of the molybdenum center as the site of irreversible inactivation by ferricyanide. 
  • Human Factor XIII from plasma and platelets. Molecular weights, subunit structures, proteolytic activation, and cross-linking of fibrinogen and fibrin. 
  • Human PXR forms a tryptophan zipper-mediated homodimer. 
  • Human aqueous humor exosomes. 
  • Influence of lipid composition on physical properties and peg-mediated fusion of curved and uncurved model membrane vesicles: "nature's own" fusogenic lipid bilayer. 
  • Mechanistic analysis of the Saccharomyces cerevisiae kinesin Kar3. 
  • Minor RNA and other components of host origin intrinsic to avian leukosis virus particles. 
  • Modulating protein structure with fluorous amino acids: increased stability and native-like structure conferred on a 4-helix bundle protein by hexafluoroleucine. 
  • Nuclease activity in a fragment of bacteriophage T4 deoxyribonucleic acid polymerase induced by the amber mutant am B22. 
  • Photoluminescence from inner walls in double-walled carbon nanotubes: some do, some do not. 
  • Preparation of high molecular weight invertebrate hemoglobins. 
  • Prespliceosome and spliceosome isolation and analysis. 
  • Pressure corrections for CoCl2 as a thermometer in an analytic ultracentrifuge. 
  • Protein bisoynthesis in the testis. IV. Isolation and properties of polyribosomes. 
  • Purification and characterization of cysteine synthetase, a bifunctional protein complex, from Salmonella typhimurium. 
  • Purification and properties of sulfite oxidase from chicken liver. Presence of molybdenum in sulfite oxidase from diverse sources. 
  • Reduced nicotinamide adenine dinucleotide phosphate-sulfite reductase of enterobacteria. IV. The Escherichia coli hemoflavoprotein: subunit structure and dissociation into hemoprotein and flavoprotein components. 
  • Ribosomal subunits of Landschütz ascites cells during changes in polysome distribution. 
  • Sedimentation and volume analysis of human bone marrow. 
  • Sel-association of glucagon. Equilibrium studies. 
  • Sliding clamp of the bacteriophage T4 polymerase has open and closed subunit interfaces in solution. 
  • Soluble monomeric P-selectin containing only the lectin and epidermal growth factor domains binds to P-selectin glycoprotein ligand-1 on leukocytes. 
  • Specific interaction of 3H-FSH with rat testis binding sites. 
  • Stable ribosome binding to the endoplasmic reticulum enables compartment-specific regulation of mRNA translation. 
  • Statin therapy and thromboxane generation in patients with coronary artery disease treated with high-dose aspirin. 
  • Stimulation of synthesis of ribonucleic acid in sub-apical sections of Avena coleoptile by indolyl-3-acetic acid. 
  • Structural interconversions modulate activity of Escherichia coli ribonucleotide reductase. 
  • Studies on alpha-actinin-like proteins liberated during trypsin digestion of alpha-actinin and of myofibrils. 
  • The chemokines IL-8, monocyte chemoattractant protein-1, and I-309 are monomers at physiologically relevant concentrations. 
  • The enzymic synthesis of L-cysteine in Escherichia coli and Salmonella typhimurium. 
  • The flow of aqueous humor through micro-porous filters. 
  • The hemoglobin of the crossopterygian fish, Latimeria chalumnae (Smith). Subunit structure and oxygen equilibria. 
  • The stoichiometry of trimeric SIV glycoprotein interaction with CD4 differs from that of anti-envelope antibody Fab fragments. 
  • The subunits in rabbit anti-Forssman IgM antibody. 
  • Total RNA isolation by a rapid centrifugation method. 
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  Keywords of People

  • Erickson, Harold Paul, James B. Duke Professor of Cell Biology, Biomedical Engineering
  • Ferguson, P. Lee, Associate Professor of Civil and Environmental Engineering, Civil and Environmental Engineering
  • Hogan, Brigid L. M., George Barth Geller Professor, Cell Biology