Xenopus

• 

  Subject Areas on Research

  • A BMP pathway regulates cell fate allocation along the sea urchin animal-vegetal embryonic axis. 
  • A Wnt-CKIvarepsilon-Rap1 pathway regulates gastrulation by modulating SIPA1L1, a Rap GTPase activating protein. 
  • A human protein related to yeast Cdc6p. 
  • A mouse homologue of FAST-1 transduces TGF beta superfamily signals and is expressed during early embryogenesis. 
  • A role for Cdc2- and PP2A-mediated regulation of Emi2 in the maintenance of CSF arrest. 
  • A role for PP1 in the Cdc2/Cyclin B-mediated positive feedback activation of Cdc25. 
  • A voltage-gated calcium-selective channel encoded by a sodium channel-like gene. 
  • Activation of the cloned muscarinic potassium channel by G protein beta gamma subunits. 
  • Activation, desensitization, and recycling of frog erythrocyte beta-adrenergic receptors. Differential perturbation by in situ trypsinization. 
  • Activation-dependent subconductance levels in the drk1 K channel suggest a subunit basis for ion permeation and gating. 
  • Alternative splicing of an insect sodium channel gene generates pharmacologically distinct sodium channels. 
  • An alanine residue in the M3-M4 linker lines the glycine binding pocket of the N-methyl-D-aspartate receptor. 
  • An epilepsy/dyskinesia-associated mutation enhances BK channel activation by potentiating Ca2+ sensing. 
  • Analysis of apoptosis using Xenopus egg extracts. 
  • Ankyrin-B is required for coordinated expression of beta-2-spectrin, the Na/K-ATPase and the Na/Ca exchanger in the inner segment of rod photoreceptors. 
  • Antibodies raised against purified beta-adrenergic receptors specifically bind beta-adrenergic ligands. 
  • Apoptosis induction by caspase-8 is amplified through the mitochondrial release of cytochrome c. 
  • Aquaporins and ion conductance. 
  • Aquaporins in Saccharomyces. Genetic and functional distinctions between laboratory and wild-type strains. 
  • Arsenite transport by mammalian aquaglyceroporins AQP7 and AQP9. 
  • Aven-dependent activation of ATM following DNA damage. 
  • Bcr-Abl-mediated protection from apoptosis downstream of mitochondrial cytochrome c release. 
  • Biochemical characterization of Leishmania major aquaglyceroporin LmAQP1: possible role in volume regulation and osmotaxis. 
  • Bone morphogenetic protein-4 (BMP-4) acts during gastrula stages to cause ventralization of Xenopus embryos. 
  • CRISPR-SID: Identifying EZH2 as a druggable target for desmoid tumors via in vivo dependency mapping. 
  • Ca(2+)/calmodulin regulates Kvβ1.1-mediated inactivation of voltage-gated K(+) channels. 
  • Calcium channel function regulated by the SH3-GK module in beta subunits. 
  • Calmodulin supports both inactivation and facilitation of L-type calcium channels. 
  • Cell cycle control during early embryogenesis. 
  • Characterization of proteins that interact with the cell-cycle regulatory protein Ran/TC4. 
  • Checkpoint signals in grasshopper meiosis are sensitive to microtubule attachment, but tension is still essential. 
  • Chloride dependence of hyperpolarization-activated chloride channel gates. 
  • Comparison of human and Xenopus GATA-2 promoters. 
  • Conformational studies of the nucleic acid binding sites for Xenopus transcription factor IIIA. 
  • Control of Emi2 activity and stability through Mos-mediated recruitment of PP2A. 
  • Control of cyclin B1 localization through regulated binding of the nuclear export factor CRM1. 
  • Crk is required for apoptosis in Xenopus egg extracts. 
  • Cytoskeletal actin gene families of Xenopus borealis and Xenopus laevis. 
  • Defining fibronectin's cell adhesion synergy site by site-directed mutagenesis. 
  • Different responses to repeated applications of zingerone in behavioral studies, recordings from intact and cultured TG neurons, and from VR1 receptors. 
  • Differential effects of TipE and a TipE-homologous protein on modulation of gating properties of sodium channels from Drosophila melanogaster. 
  • Directed evolution of the surface chemistry of the reporter enzyme beta-glucuronidase. 
  • Disease-associated casein kinase I delta mutation may promote adenomatous polyps formation via a Wnt/beta-catenin independent mechanism. 
  • Distinct modulating effects of TipE-homologs 2-4 on Drosophila sodium channel splice variants. 
  • Distinct roles of the DmNav and DSC1 channels in the action of DDT and pyrethroids. 
  • Domain-specific antibodies reveal multiple-site topology of Nup153 within the nuclear pore complex. 
  • Dorsalizing and neuralizing properties of Xdsh, a maternally expressed Xenopus homolog of dishevelled. 
  • Embryonic expression of Lim-1, the mouse homolog of Xenopus Xlim-1, suggests a role in lateral mesoderm differentiation and neurogenesis. 
  • Emi2-mediated inhibition of E2-substrate ubiquitin transfer by the anaphase-promoting complex/cyclosome through a D-box-independent mechanism. 
  • Enantiomeric propanolamines as selective N-methyl-D-aspartate 2B receptor antagonists. 
  • Eukaryotic gene transcription with purified components. 
  • Evidence for a dual role for TC4 protein in regulating nuclear structure and cell cycle progression. 
  • Expression of TGF-beta-related genes during mouse embryo whisker morphogenesis. 
  • Expression of an abundant alternatively spliced form of the cystic fibrosis transmembrane conductance regulator (CFTR) gene is not associated with a cAMP-activated chloride conductance. 
  • FXR1 splicing is important for muscle development and biomolecular condensates in muscle cells. 
  • Fast and slow gating of sodium channels encoded by a single mRNA. 
  • Formation of a rate-limiting intermediate in 5S RNA gene transcription. 
  • Forskolin stimulation of water and cation permeability in aquaporin 1 water channels. 
  • Frog oocytes synthesize and completely process the precursor polypeptide to virion structural proteins after microinjection of avian myeloblastosis virus RNA. 
  • Functional activity and regulation of human beta 2-adrenergic receptors expressed in Xenopus oocytes. 
  • Functional expression of an arachnid sodium channel reveals residues responsible for tetrodotoxin resistance in invertebrate sodium channels. 
  • Gating currents from a delayed rectifier K+ channel with altered pore structure and function. 
  • Genetic elucidation of human hyperosmia to isovaleric acid. 
  • Getting under—and through—the skin: ecological genomics of chytridiomycosis infection in frogs. 
  • Identification of a role for beta-catenin in the establishment of a bipolar mitotic spindle. 
  • Identification of the components controlling inactivation of voltage-gated Ca2+ channels. 
  • Identification of the nuclear localization signal in Xenopus cyclin E and analysis of its role in replication and mitosis. 
  • Impact of intracellular hemin on N-type inactivation of voltage-gated K+ channels. 
  • In vitro cell cycle arrest induced by using artificial DNA templates. 
  • Inhibition of the anaphase-promoting complex by the Xnf7 ubiquitin ligase. 
  • Inhibitors of metalloendoprotease activity prevent K+-stimulated neurotransmitter release from the retina of Xenopus laevis. 
  • Insight into the Mode of Action of Haedoxan A from Phryma leptostachya. 
  • Intron retention in mRNA encoding ancillary subunit of insect voltage-gated sodium channel modulates channel expression, gating regulation and drug sensitivity. 
  • KCNQ1 assembly and function is blocked by long-QT syndrome mutations that disrupt interaction with calmodulin. 
  • Kinetics of protein import into isolated Xenopus oocyte nuclei. 
  • Mapping the interaction surface of scorpion β-toxins with an insect sodium channel. 
  • Mcm10 and And-1/CTF4 recruit DNA polymerase alpha to chromatin for initiation of DNA replication. 
  • Meiosis, mitosis and microtubule motors. 
  • Membrane localization of the kinase which phosphorylates p34cdc2 on threonine 14. 
  • Metabolic control of oocyte apoptosis mediated by 14-3-3zeta-regulated dephosphorylation of caspase-2. 
  • Microtubule cross-linking triggers the directional motility of kinesin-5. 
  • Mitochondrial localization of Reaper to promote inhibitors of apoptosis protein degradation conferred by GH3 domain-lipid interactions. 
  • Modulation of Xenopus oocyte-expressed phospholemman-induced ion currents by co-expression of protein kinases. 
  • Molecular Mechanism of Action and Selectivity of Sodium Ch annel Blocker Insecticides. 
  • Molecular and functional characterization of a novel sodium channel TipE-like auxiliary subunit from the American cockroach Periplaneta americana. 
  • Molecular and functional characterization of voltage-gated sodium channel variants from Drosophila melanogaster. 
  • Molecular basis of calmodulin tethering and Ca2+-dependent inactivation of L-type Ca2+ channels. 
  • Molecular biology of adrenergic receptors. 
  • Molecular evidence for dual pyrethroid-receptor sites on a mosquito sodium channel. 
  • Molecular evidence of sequential evolution of DDT- and pyrethroid-resistant sodium channel in Aedes aegypti. 
  • Molecular morphogens. Upside-down ideas vindicated. 
  • Mutations in the transmembrane helix S6 of domain IV confer cockroach sodium channel resistance to sodium channel blocker insecticides and local anesthetics. 
  • Mutations of two acidic residues at the cytoplasmic end of segment IIIS6 of an insect sodium channel have distinct effects on pyrethroid resistance. 
  • Negative regulation of DNA replication by the retinoblastoma protein is mediated by its association with MCM7. 
  • Nicotine dependence is associated with functional variation in FMO3, an enzyme that metabolizes nicotine in the brain. 
  • Nodal-related signals induce axial mesoderm and dorsalize mesoderm during gastrulation. 
  • Novel sodium channel gene mutations in Blattella germanica reduce the sensitivity of expressed channels to deltamethrin. 
  • Nuclear import of Cdk/cyclin complexes: identification of distinct mechanisms for import of Cdk2/cyclin E and Cdc2/cyclin B1. 
  • Nuclear transport kinetics in microarrays of nuclear envelope patches. 
  • PP1 control of M phase entry exerted through 14-3-3-regulated Cdc25 dephosphorylation. 
  • Pertussis-toxin-sensitive Galpha subunits selectively bind to C-terminal domain of neuronal GIRK channels: evidence for a heterotrimeric G-protein-channel complex. 
  • Phylogenetic analysis of vertebrate lactate dehydrogenase (LDH) multigene families. 
  • Pore region of TRPV3 ion channel is specifically required for heat activation. 
  • Positional cloning of zebrafish ferroportin1 identifies a conserved vertebrate iron exporter. 
  • Preparation and use of interphase Xenopus egg extracts. 
  • Properties of the beta 1- and beta 2-adrenergic receptor subtypes revealed by molecular cloning. 
  • Protein phosphatase 1 regulates assembly and function of the beta-catenin degradation complex. 
  • Protein phosphatase 1 regulates the stability of the circadian protein PER2. 
  • Purification and molecular cloning of a secreted, Frizzled-related antagonist of Wnt action. 
  • RCC1, a regulator of mitosis, is essential for DNA replication. 
  • RNA editing generates tissue-specific sodium channels with distinct gating properties. 
  • Reaper-induced apoptosis in a vertebrate system. 
  • Reaper-induced dissociation of a Scythe-sequestered cytochrome c-releasing activity. 
  • Reconstitution of beta-adrenergic receptors in lipid vesicles: affinity chromatography-purified receptors confer catecholamine responsiveness on a heterologous adenylate cyclase system. 
  • Reconstitution of nuclear protein export in isolated nuclear envelopes. 
  • Regulation of apoptosis in Xenopus egg extracts. 
  • Regulation of beta-catenin signaling by the B56 subunit of protein phosphatase 2A. 
  • Regulation of casein kinase I epsilon activity by Wnt signaling. 
  • Rem2 is an activity-dependent negative regulator of dendritic complexity in vivo. 
  • Restraint of apoptosis during mitosis through interdomain phosphorylation of caspase-2. 
  • Reversible inhibition of Hsp70 chaperone function by Scythe and Reaper. 
  • Role for the PP2A/B56delta phosphatase in regulating 14-3-3 release from Cdc25 to control mitosis. 
  • Scythe: a novel reaper-binding apoptotic regulator. 
  • Sensing and Processing of DNA Interstrand Crosslinks by the Mismatch Repair Pathway. 
  • Sequence variations at I260 and A1731 contribute to persistent currents in Drosophila sodium channels. 
  • Site-specific casein kinase 1epsilon-dependent phosphorylation of Dishevelled modulates beta-catenin signaling. 
  • Smad5 induces ventral fates in Xenopus embryo. 
  • Steering growth cones with a CaMKII/calcineurin switch. 
  • Structural cues involved in endoplasmic reticulum degradation of G85E and G91R mutant cystic fibrosis transmembrane conductance regulator. 
  • Study of apoptosis in vitro using the Xenopus egg extract reconstitution system. 
  • Substitutions in the domain III voltage-sensing module enhance the sensitivity of an insect sodium channel to a scorpion beta-toxin. 
  • Synthesis and complete processing of a high-molecular-weight precursor polypeptide to viral structural proteins in toad oocytes micro-injected with avian myeloblastosis virus ribonucleic acid [proceedings]. 
  • TBX3 Directs Cell-Fate Decision toward Mesendoderm. 
  • TBX5 is required for embryonic cardiac cell cycle progression. 
  • TGF-beta related genes in development. 
  • TMEM16F forms a Ca2+-activated cation channel required for lipid scrambling in platelets during blood coagulation. 
  • Tetraethylammonium block of water flux in Aquaporin-1 channels expressed in kidney thin limbs of Henle's loop and a kidney-derived cell line. 
  • Tetramerization and single-stranded DNA binding properties of native and mutated forms of murine mitochondrial single-stranded DNA-binding proteins. 
  • The COPI complex functions in nuclear envelope breakdown and is recruited by the nucleoporin Nup153. 
  • The GARP Domain of the Rod CNG Channel's β1-Subunit Contains Distinct Sites for Outer Segment Targeting and Connecting to the Photoreceptor Disk Rim. 
  • The bipolar mitotic kinesin Eg5 moves on both microtubules that it crosslinks. 
  • The canonical Wnt pathway in embryonic axis polarity. 
  • The genetic regulation of pigment cell development. 
  • The mitotic peptidyl-prolyl isomerase, Pin1, interacts with Cdc25 and Plx1. 
  • The role of Dbf4-dependent protein kinase in DNA polymerase ζ-dependent mutagenesis in Saccharomyces cerevisiae. 
  • Translation of 35S RNA from avian myeloblastosis virus into viral specific polypeptides in Xenopus oocytes. 
  • Ubiquitin-mediated degradation a mechanism for fine-tuning TGF-beta signaling. 
  • Unwinding the Wnt action of casein kinase 1. 
  • Valproate reduces excitability by blockage of sodium and potassium conductance. 
  • Vgr-1, a mammalian gene related to Xenopus Vg-1, is a member of the transforming growth factor beta gene superfamily. 
  • WLS-dependent secretion of WNT3A requires Ser209 acylation and vacuolar acidification. 
  • When the checkpoints have gone: insights into Cdc25 functional activation. 
  • Wound healing: The power of the purse string. 
  • XMAP215 is a long thin molecule that does not increase microtubule stiffness. 
  • Xenopus Kazrin interacts with ARVCF-catenin, spectrin and p190B RhoGAP, and modulates RhoA activity and epithelial integrity. 
  • pH dependence of the Slc4a11-mediated H⁺ conductance is influenced by intracellular lysine residues and modified by disease-linked mutations. 
• 

  Keywords of People

  • Carbrey, Jennifer Mehlman, Assistant Research Professor of Cell Biology, Cell Biology
  • Erickson, Harold Paul, James B. Duke Distinguished Professor Emeritus, Cell Biology
  • Hogan, Brigid L. M., Research Professor of Cell Biology, Cell Biology
  • Pitt, Geoffrey Stuart, Adjunct Professor in the Department of Medicine, Medicine, Cardiology