Xenopus laevis

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  Subject Areas on Research

  • A Xenopus egg factor with DNA-binding properties characteristic of terminus-specific telomeric proteins. 
  • A charged residue in S4 regulates coupling among the activation gate, voltage, and Ca2+ sensors in BK channels. 
  • A comprehensive search for calcium binding sites critical for TMEM16A calcium-activated chloride channel activity. 
  • A dimerized coiled-coil domain and an adjoining part of geminin interact with two sites on Cdt1 for replication inhibition. 
  • A feedback loop in the polo-like kinase activation pathway. 
  • A mutation in the glycine binding pocket of the N-methyl-D-aspartate receptor NR1 subunit alters agonist efficacy. 
  • A mutation in the intracellular loop III/IV of mosquito sodium channel synergizes the effect of mutations in helix IIS6 on pyrethroid resistance. 
  • A novel potassium channel with delayed rectifier properties isolated from rat brain by expression cloning. 
  • A residue in the transmembrane segment 6 of domain I in insect and mammalian sodium channels regulate differential sensitivities to pyrethroid insecticides. 
  • A sodium channel mutation identified in Aedes aegypti selectively reduces cockroach sodium channel sensitivity to type I, but not type II pyrethroids. 
  • A truncated form of fibroblast growth factor receptor 1 inhibits signal transduction by multiple types of fibroblast growth factor receptor. 
  • Abnormal creatine transport of mutations in monocarboxylate transporter 12 (MCT12) found in patients with age-related cataract can be partially rescued by exogenous chaperone CD147. 
  • Action of six pyrethrins purified from the botanical insecticide pyrethrum on cockroach sodium channels expressed in Xenopus oocytes. 
  • Adrenergic receptors: molecular mechanisms of clinically relevant regulation. 
  • Alanine to valine substitutions in the pore helix IIIP1 and linker-helix IIIL45 confer cockroach sodium channel resistance to DDT and pyrethroids. 
  • Allosteric effects of external K+ ions mediated by the aspartate of the GYGD signature sequence in the Kv2.1 K+ channel. 
  • Allosteric inhibition of kinesin-5 modulates its processive directional motility. 
  • An important role of a pyrethroid-sensing residue F1519 in the action of the N-alkylamide insecticide BTG 502 on the cockroach sodium channel. 
  • Analysis of the action of lidocaine on insect sodium channels. 
  • Analysis of the cell cycle using Xenopus egg extracts. 
  • Ankyrin-G promotes cyclic nucleotide-gated channel transport to rod photoreceptor sensory cilia. 
  • Antiidiotypic antibody recognizes an amiloride binding domain within the alpha subunit of the epithelial Na+ channel. 
  • Apoptosis in Xenopus egg extracts. 
  • Aquaglyceroporin AQP9: solute permeation and metabolic control of expression in liver. 
  • Aquaporins in Saccharomyces: Characterization of a second functional water channel protein. 
  • Arsenic trioxide uptake by human and rat aquaglyceroporins. 
  • Beta-adrenergic receptors: biochemical mechanisms of physiological regulation. 
  • Bradykinin and nerve growth factor release the capsaicin receptor from PtdIns(4,5)P2-mediated inhibition. 
  • CaMKII tethers to L-type Ca2+ channels, establishing a local and dedicated integrator of Ca2+ signals for facilitation. 
  • Calcium-sensitive potassium channelopathy in human epilepsy and paroxysmal movement disorder. 
  • Casein kinase I in the mammalian circadian clock. 
  • Casein kinase I phosphorylates and destabilizes the beta-catenin degradation complex. 
  • Caspase-3 cleavage links delta-catenin to the novel nuclear protein ZIFCAT. 
  • Cathepsin S causes inflammatory pain via biased agonism of PAR2 and TRPV4. 
  • Changes in the pattern of adherens junction-associated beta-catenin accompany morphogenesis in the sea urchin embryo. 
  • Combinatorial control of cyclin B1 nuclear trafficking through phosphorylation at multiple sites. 
  • Cone outer segments: a biophysical model of membrane dynamics, shape retention, and lamella formation. 
  • Constitutive activation of the N-methyl-D-aspartate receptor via cleft-spanning disulfide bonds. 
  • Control of cyclin B1 localization through regulated binding of the nuclear export factor CRM1. 
  • Coupling of the expressed alpha 1B-adrenergic receptor to the phospholipase C pathway in Xenopus oocytes. The role of Go. 
  • Cyclin-binding motifs are essential for the function of p21CIP1. 
  • Cytoskeletal actin gene families of Xenopus borealis and Xenopus laevis. 
  • DNA replication checkpoint control of Wee1 stability by vertebrate Hsl7. 
  • DVR-4 (bone morphogenetic protein-4) as a posterior-ventralizing factor in Xenopus mesoderm induction. 
  • Direct demonstration of impaired functionality of a purified desensitized beta-adrenergic receptor in a reconstituted system. 
  • Discovery of novel ligands for mouse olfactory receptor MOR42-3 using an in silico screening approach and in vitro validation. 
  • Dorsalizing and neuralizing properties of Xdsh, a maternally expressed Xenopus homolog of dishevelled. 
  • Dose-dependent and isoform-specific modulation of Ca2+ channels by RGK GTPases. 
  • EB1-microtubule interactions in Xenopus egg extracts: role of EB1 in microtubule stabilization and mechanisms of targeting to microtubules. 
  • Effects of multiple metal binding sites on calcium and magnesium-dependent activation of BK channels. 
  • Essential Ca(V)beta modulatory properties are AID-independent. 
  • Evidence that direct binding of G beta gamma to the GIRK1 G protein-gated inwardly rectifying K+ channel is important for channel activation. 
  • Facile synthesis of site-specifically acetylated and methylated histone proteins: reagents for evaluation of the histone code hypothesis. 
  • Facilitative glucose transporter Glut1 is actively excluded from rod outer segments. 
  • Features of programmed cell death in intact Xenopus oocytes and early embryos revealed by near-infrared fluorescence and real-time monitoring. 
  • Functional analysis of a mammalian odorant receptor subfamily. 
  • Functional integrity of desensitized beta-adrenergic receptors. 
  • Homologous desensitization of the beta-adrenergic receptor. Functional integrity of the desensitized receptor from mammalian lung. 
  • Identification of a novel, multifunctional beta-defensin (human beta-defensin 3) with specific antimicrobial activity. Its interaction with plasma membranes of Xenopus oocytes and the induction of macrophage chemoattraction. 
  • Identification of a regulated Na/K/Cl cotransport system in a distal nephron cell line. 
  • Identification of deacetylase substrates with the biotin switch approach. 
  • Identification of genes expressed in the Xenopus inner ear. 
  • Identification of new batrachotoxin-sensing residues in segment IIIS6 of the sodium channel. 
  • Interaction between residues in the Mg2+-binding site regulates BK channel activation. 
  • Interactions between propofol and lipid mediator receptors: inhibition of lysophosphatidate signaling. 
  • K channel subconductance levels result from heteromeric pore conformations. 
  • Ligand-binding residues integrate affinity and efficacy in the NMDA receptor. 
  • Load-dependent release limits the processive stepping of the tetrameric Eg5 motor. 
  • Maintenance of G2 arrest in the Xenopus oocyte: a role for 14-3-3-mediated inhibition of Cdc25 nuclear import. 
  • Metabolic control of Ca2+/calmodulin-dependent protein kinase II (CaMKII)-mediated caspase-2 suppression by the B55β/protein phosphatase 2A (PP2A). 
  • Metabolic regulation of oocyte cell death through the CaMKII-mediated phosphorylation of caspase-2. 
  • Metabolomic profiling reveals a role for caspase-2 in lipoapoptosis. 
  • Microinjection of a 19-kDa guanine nucleotide-binding protein inhibits maturation of Xenopus oocytes. 
  • Microtubules accelerate the kinase activity of Aurora-B by a reduction in dimensionality. 
  • Missense mutation (G480C) in the CFTR gene associated with protein mislocalization but normal chloride channel activity. 
  • Mitochondrial ROS cause motor deficits induced by synaptic inactivity: Implications for synapse pruning. 
  • Molecular basis of differential sensitivity of insect sodium channels to DCJW, a bioactive metabolite of the oxadiazine insecticide indoxacarb. 
  • Molecular basis of selective resistance of the bumblebee BiNa_v 1 sodium channel to tau-fluvalinate. 
  • Molecular characterization and functional expression of the DSC1 channel. 
  • Molecular dissection of gating in the ClC-2 chloride channel. 
  • Msx1 and Pax3 cooperate to mediate FGF8 and WNT signals during Xenopus neural crest induction. 
  • Multiscale computational analysis of Xenopus laevis morphogenesis reveals key insights of systems-level behavior. 
  • Mutagenic analysis of functional domains of the mos proto-oncogene and identification of the sites important for MAPK activation and DNA binding. 
  • Neurosteroid transport by the organic solute transporter OSTα-OSTβ. 
  • Neutrophil Elastase Activates Protease-activated Receptor-2 (PAR2) and Transient Receptor Potential Vanilloid 4 (TRPV4) to Cause Inflammation and Pain. 
  • Nomenclature: vertebrate mediators of TGFbeta family signals. 
  • Nuclear export of mammalian PERIOD proteins. 
  • PP1-mediated dephosphorylation of phosphoproteins at mitotic exit is controlled by inhibitor-1 and PP1 phosphorylation. 
  • Phosphatases driving mitosis: pushing the gas and lifting the brakes. 
  • Phosphorylation and nuclear translocation of a regulator of G protein signaling (RGS10). 
  • Phosphorylation of the cyclin b1 cytoplasmic retention sequence by mitogen-activated protein kinase and Plx. 
  • Polarization of a spherical cell in a nonuniform extracellular electric field. 
  • Post-cytochrome C protection from apoptosis conferred by a MAPK pathway in Xenopus egg extracts. 
  • Precise regulation of porcupine activity is required for physiological Wnt signaling. 
  • Properties of the Arg376 residue of the proton-coupled folate transporter (PCFT-SLC46A1) and a glutamine mutant causing hereditary folate malabsorption. 
  • Protein phosphatase 2A and its B56 regulatory subunit inhibit Wnt signaling in Xenopus. 
  • Pure beta-adrenergic receptor: the single polypeptide confers catecholamine responsiveness to adenylate cyclase. 
  • Reaper eliminates IAP proteins through stimulated IAP degradation and generalized translational inhibition. 
  • Regulation of biliary secretion through apical purinergic receptors in cultured rat cholangiocytes. 
  • Regulation of the ATM-activator protein Aven by CRM1-dependent nuclear export. 
  • Requirement of the prolyl isomerase Pin1 for the replication checkpoint. 
  • Revisiting the Role of Ser982 Phosphorylation in Stoichiometry Shift of the Electrogenic Na+/qHCO3- Cotransporter NBCe1. 
  • Role of the sixth transmembrane segment of domain IV of the cockroach sodium channel in the action of sodium channel blocker insecticides. 
  • SHP-2 is required for the maintenance of cardiac progenitors. 
  • Sally Kornbluth: Nature's incredible contraptions. 
  • Sequence- or position-specific mutations in the carboxyl-terminal FL motif of the kidney sodium bicarbonate cotransporter (NBC1) disrupt its basolateral targeting and alpha-helical structure. 
  • Site-directed mutagenesis of the cytoplasmic domains of the human beta 2-adrenergic receptor. Localization of regions involved in G protein-receptor coupling. 
  • Spectral triangulation molecular contrast optical coherence tomography with indocyanine green as the contrast agent. 
  • Stimulation of aquaporin-mediated fluid transport by cyclic GMP in human retinal pigment epithelium in vitro. 
  • Structural basis of nucleoside and nucleoside drug selectivity by concentrative nucleoside transporters. 
  • Structural conservation of ion conduction pathways in K channels and glutamate receptors. 
  • Subunit-specific effect of the voltage sensor domain on Ca2+ sensitivity of BK channels. 
  • Suppression of DNA-damage checkpoint signaling by Rsk-mediated phosphorylation of Mre11. 
  • Tbx5 and Tbx20 act synergistically to control vertebrate heart morphogenesis. 
  • The 5'-untranslated region of the N-methyl-D-aspartate receptor NR2A subunit controls efficiency of translation. 
  • The CapZ interacting protein Rcsd1 is required for cardiogenesis downstream of Wnt11a in Xenopus laevis. 
  • The DVR gene family in embryonic development. 
  • The NMDA receptor M3 segment is a conserved transduction element coupling ligand binding to channel opening. 
  • The NR1 M3 domain mediates allosteric coupling in the N-methyl-D-aspartate receptor. 
  • The Wnt/JNK signaling target gene alcam is required for embryonic kidney development. 
  • The activation mechanism of rat vanilloid receptor 1 by capsaicin involves the pore domain and differs from the activation by either acid or heat. 
  • The effect of monastrol on the processive motility of a dimeric kinesin-5 head/kinesin-1 stalk chimera. 
  • The interface between membrane-spanning and cytosolic domains in Ca²+-dependent K+ channels is involved in β subunit modulation of gating. 
  • The molecular basis for ligand specificity in a mouse olfactory receptor: a network of functionally important residues. 
  • The outer segment serves as a default destination for the trafficking of membrane proteins in photoreceptors. 
  • The potassium channel MBK1 (Kv1.1) is expressed in the mouse retina. 
  • The vesicular glutamate transporter 1 (xVGlut1) is expressed in discrete regions of the developing Xenopus laevis nervous system. 
  • Toxin and kinetic profile of rat brain type III sodium channels expressed in Xenopus oocytes. 
  • Trophic effects of androgen: development and hormonal regulation of neuron number in a sexually dimorphic vocal motor nucleus. 
  • Tuning magnesium sensitivity of BK channels by mutations. 
  • Two forms of mitochondrial DNA ligase III are produced in Xenopus laevis oocytes. 
  • Two separate interfaces between the voltage sensor and pore are required for the function of voltage-dependent K(+) channels. 
  • Vasoactive intestinal peptide, forskolin, and genistein increase apical CFTR trafficking in the rectal gland of the spiny dogfish, Squalus acanthias. Acute regulation of CFTR trafficking in an intact epithelium. 
  • Viral sequences enable efficient and tissue-specific expression of transgenes in Xenopus. 
  • Voltage-dependent action of valproate on potassium channels in frog node of Ranvier. 
  • WLS retrograde transport to the endoplasmic reticulum during Wnt secretion. 
  • WT1 and Sox11 regulate synergistically the promoter of the Wnt4 gene that encodes a critical signal for nephrogenesis. 
  • Wee1-regulated apoptosis mediated by the crk adaptor protein in Xenopus egg extracts. 
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  Keywords of People

  • Carbrey, Jennifer Mehlman, Assistant Research Professor of Cell Biology, Cell Biology
  • Pitt, Geoffrey Stuart, Adjunct Professor in the Department of Medicine, Medicine, Cardiology