Blotting, Southern

• 

  Subject Areas on Research

  • 2',3'-dideoxy-beta-L-5-fluorocytidine inhibits duck hepatitis B virus reverse transcription and suppresses viral DNA synthesis in hepatocytes, both in vitro and in vivo. 
  • A deletion unit on chromosome 17q in epithelial ovarian tumors distal to the familial breast/ovarian cancer locus. 
  • A fragile X male with a broad smear on Southern blot analysis representing 100-500 CGG repeats and no methylation at the EagI site of the FMR-1 gene. 
  • A functional isoform of the human granulocyte/macrophage colony-stimulating factor receptor has an unusual cytoplasmic domain. 
  • A functional mouse ornithine decarboxylase gene (Odc) maps to chromosome 12: further evidence of homoeology between mouse chromosome 12 and the short arm of human chromosome 2. 
  • A high proportion of ADA point mutations associated with a specific alanine-to-valine substitution. 
  • A method for analysis of gene expression patterns. 
  • A radiation hybrid map of the distal short arm of human chromosome 11, containing the Beckwith-Wiedemann and associated embryonal tumor disease loci. 
  • A suppressor/enhancer screen in Drosophila reveals a role for wnt-mediated lipid metabolism in primordial germ cell migration. 
  • A tyrosine hydroxylase-yellow fluorescent protein knock-in reporter system labeling dopaminergic neurons reveals potential regulatory role for the first intron of the rodent tyrosine hydroxylase gene. 
  • Aberrant splicing of the TSG101 tumor suppressor gene in human breast and ovarian cancers. 
  • Abl-interactor-1 (Abi1) has a role in cardiovascular and placental development and is a binding partner of the alpha4 integrin. 
  • Absent ras gene mutations in human adrenal cortical neoplasms and pheochromocytomas. 
  • Activating mutations of Gs protein in monostotic fibrous lesions of bone. 
  • Adrenergic receptor homologies in vertebrate and invertebrate species examined by DNA hybridization. 
  • Altered growth of a human neuroendocrine carcinoma line after transfection of a major histocompatibility complex class I gene. 
  • Amendment to Clinical Research Project. Project 90-C-195. April 1, 1993. Treatment of severe combined immunodeficiency disease (SCID) due to adenosine deaminase deficiency with autologous lymphocytes transduced with a human ADA gene. 
  • Amplification of HER-2/neu gene in human gastric adenocarcinomas: correlation with primary site. 
  • Amplification of telomeric DNA directly correlates with metastatic potential of human and murine cancers of various histological origin. 
  • An Arabidopsis thaliana lipoxygenase gene can be induced by pathogens, abscisic acid, and methyl jasmonate. 
  • An extensive de novo deletion removing FMR1 in a patient with mental retardation and the fragile X syndrome phenotype. 
  • An integrated physical map of 210 markers assigned to the short arm of human chromosome 11. 
  • Analytical DNA fingerprinting in lions: parentage, genetic diversity, and kinship. 
  • Assessing a role for enhancer-blocking activity in gene regulation within the murine T-cell receptor alpha/delta locus. 
  • Assignment of the human glycogen debrancher gene to chromosome 1p21. 
  • Bacteriophage T4 initiates bidirectional DNA replication through a two-step process. 
  • Catecholaminergic cell lines from the brain and adrenal glands of tyrosine hydroxylase-SV40 T antigen transgenic mice. 
  • Characterization of human brain cDNA encoding the general isoform of beta-spectrin. 
  • Characterization of the mechanisms of busulfan resistance in a human glioblastoma multiforme xenograft. 
  • Chromosomal localization of gene for human glutamate receptor subunit-7. 
  • Chromosomal organization of adrenergic receptor genes. 
  • Chromosomal organization of rRNA operons in Bacillus subtilis. 
  • Classification and genetic characterization of pattern-forming Bacilli. 
  • Cloning of a complementary DNA encoding a new mouse B lymphocyte differentiation antigen, homologous to the human B1 (CD20) antigen, and localization of the gene to chromosome 19. 
  • Cloning of an NF-kappa B subunit which stimulates HIV transcription in synergy with p65. 
  • Cloning of cDNA and analysis of the gene for mouse angiotensin II type 2 receptor. 
  • Collagenase 1 and collagenase 3 expression in a guinea pig model of osteoarthritis. 
  • Comparison of myristoyl-CoA:protein N-myristoyltransferases from three pathogenic fungi: Cryptococcus neoformans, Histoplasma capsulatum, and Candida albicans. 
  • Consistent production of transgenic chickens using replication-deficient retroviral vectors and high-throughput screening procedures. 
  • Control elements within the PWS/AS imprinting box and their function in the imprinting process. 
  • Cryptococcus neoformans differential gene expression detected in vitro and in vivo with green fluorescent protein. 
  • Cryptococcus neoformans mitochondrial superoxide dismutase: an essential link between antioxidant function and high-temperature growth. 
  • Curing Saccharomyces cerevisiae of the 2 micron plasmid by targeted DNA damage. 
  • DNA methyltransferase levels and altered CpG methylation in the total genome and in the GSTP1 gene in human glioma cells transfected with sense and antisense DNA methyltransferase cDNA. 
  • Deletions and rearrangements inactivate the p16INK4 gene in human glioma cells. 
  • Detection of RAS mutations in archival testicular germ cell tumors by polymerase chain reaction and oligonucleotide hybridization. 
  • Differential expression and regulation of the glucokinase gene in liver and islets of Langerhans. 
  • Differential expression of ros oncogene in primary human astrocytomas and astrocytoma cell lines. 
  • Differential inactivation of CDKN2 and Rb protein in non-small-cell and small-cell lung cancer cell lines. 
  • Differential induction of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase genes in Arabidopsis thaliana by wounding and pathogenic attack. 
  • Differential screening of a human chromosome 3 library identifies hepatocyte growth factor-like/macrophage-stimulating protein and its receptor in injured lung. Possible implications for neuroendocrine cell survival. 
  • Direct cloning of DNA sequences from the common fragile site region at chromosome band 3p14.2. 
  • Disease Expression and Familial Transmission of Fuchs Endothelial Corneal Dystrophy With and Without CTG18.1 Expansion. 
  • Distinct stress responses of two functional laccases in Cryptococcus neoformans are revealed in the absence of the thiol-specific antioxidant Tsa1. 
  • Diversity and organization of human T cell receptor delta variable gene segments. 
  • Double-strand break repair in tandem repeats during bacteriophage T4 infection. 
  • Effects of the lpr mutation on elimination and inactivation of self-reactive B cells. 
  • Ehlers-Danlos syndrome type VI results from a nonsense mutation and a splice site-mediated exon-skipping mutation in the lysyl hydroxylase gene. 
  • Enhanced green fluorescent protein as a marker for localizing murine cytomegalovirus in acute and latent infection. 
  • Enzymes that counteract nitrosative stress promote fungal virulence. 
  • Epstein-Barr virus DNA is abundant and monoclonal in the Reed-Sternberg cells of Hodgkin's disease: association with mixed cellularity subtype and Hispanic American ethnicity. 
  • Equalizing cDNA subtraction based on selective suppression of polymerase chain reaction: cloning of Jurkat cell transcripts induced by phytohemaglutinin and phorbol 12-myristate 13-acetate. 
  • Evidence for coincident mutations in human lymphoblast clones selected for functional loss of a thymidine kinase gene. 
  • Expression of O6-methylguanine-DNA methyltransferase in malignant human glioma cell lines. 
  • Expression of photosynthesis genes in relation to nitrogen fixation in the diazotrophic filamentous nonheterocystous cyanobacterium Trichodesmium sp. IMS 101. 
  • Fimbriation of Pseudomonas cepacia. 
  • Fine mapping of a putative rd cDNA and its co-segregation with rd expression. 
  • Fine-structure mapping of meiosis-specific double-strand DNA breaks at a recombination hotspot associated with an insertion of telomeric sequences upstream of the HIS4 locus in yeast. 
  • Formation and processing of stalled replication forks--utility of two-dimensional agarose gels. 
  • Frequency of homozygous deletion at p16/CDKN2 in primary human tumours. 
  • Frequent fusion of the JAZF1 and JJAZ1 genes in endometrial stromal tumors. 
  • Functional studies of the carboxy-terminal repeat domain of Drosophila RNA polymerase II in vivo. 
  • Gene dose-dependent control of hematopoiesis and hematologic tumor suppression by CBP. 
  • Gene expression of the renin-angiotensin system in human tissues. Quantitative analysis by the polymerase chain reaction. 
  • Gene modification of primary tumor cells for active immunotherapy of human breast and ovarian cancer. 
  • Gene transfer in Cryptococcus neoformans by use of biolistic delivery of DNA. 
  • Genetic basis for p53 overexpression in human breast cancer. 
  • Genetic structure and DNA sequences at junctions involved in the rearrangements of Bacillus subtilis strains carrying the trpE26 mutation. 
  • Genetically engineered large animal model for studying cone photoreceptor survival and degeneration in retinitis pigmentosa. 
  • Genome rearrangements caused by interstitial telomeric sequences in yeast. 
  • Genomic instability and endoreduplication triggered by RAD17 deletion. 
  • Genomic organization of adrenergic and serotonin receptors in the mouse: linkage mapping of sequence-related genes provides a method for examining mammalian chromosome evolution. 
  • Genomic organization, expression, and localization of murine Ran-binding protein 2 (RanBP2) gene. 
  • Genomic structure and amino acid sequence domains of the human La autoantigen. 
  • Glioblastoma-related gene mutations and over-expression of functional epidermal growth factor receptors in SKMG-3 glioma cells. 
  • HPV-16-related DNA sequences in Kaposi's sarcoma. 
  • Ha-ras polymorphisms in epithelial ovarian cancer. 
  • Human lung cancer cell lines exhibit resistance to retinoic acid treatment. 
  • Human papillomavirus types 16 and 18 are not involved in human prostate carcinogenesis: analysis of archival human prostate cancer specimens by differential polymerase chain reaction. 
  • Human type 2 angiotensin II receptor gene: cloned, mapped to the X chromosome, and its mRNA is expressed in the human lung. 
  • Identification and characterization of a Drosophila nuclear receptor with the ability to inhibit the ecdysone response. 
  • Identification and characterization of a human homolog of the Schizosaccharomyces pombe ras-like gene YPT-3. 
  • Identification of Duchenne muscular dystrophy genomic probe P20 constant Taql fragment corresponding to the EcoRV and Mspl polymorphisms. 
  • Identification of a W variant outbreak of Mycobacterium tuberculosis via population-based molecular epidemiology. 
  • Identification of a novel trimeric autotransporter adhesin in the cryptic genospecies of Haemophilus. 
  • Identification of virulence mutants of the fungal pathogen Cryptococcus neoformans using signature-tagged mutagenesis. 
  • Impact of ammonium permeases mepA, mepB, and mepC on nitrogen-regulated secondary metabolism in Fusarium fujikuroi. 
  • Improved genetic fingerprinting using RNA probes. 
  • In situ detection of Epstein-Barr virus in non-small cell lung carcinomas. 
  • Inclusion of a sensitivity control to add a quality-control parameter and improve reproducibility in BCR, immunoglobulin, and T-cell receptor gene-rearrangement studies. 
  • Inhibition of an inflammatory response is mediated by a 38-kDa protein of cowpox virus. 
  • Inositol diphosphate signaling regulates telomere length. 
  • Interaction of Cryptococcus neoformans Rim101 and protein kinase A regulates capsule. 
  • Investigation of the basis of virulence in serotype A strains of Cryptococcus neoformans from apparently immunocompetent individuals. 
  • Involvement of the TCL5 gene on human chromosome 1 in T-cell leukemia and melanoma. 
  • Isolation and characterization of the rat chromosomal gene for a polypeptide (pS1) antigenically related to statin. 
  • Isolation of a candidate cDNA for the gene causing retinal degeneration in the rd mouse. 
  • Linkage of kdr-type resistance and the para-homologous sodium channel gene in German cockroaches (Blattella germanica). 
  • Lipopolysaccharide stimulates mitochondrial biogenesis via activation of nuclear respiratory factor-1. 
  • Long- and short-lived murine hematopoietic stem cell clones individually identified with retroviral integration markers. 
  • Long-distance regulation of fetal V(δ) gene segment TRDV4 by the Tcrd enhancer. 
  • Loss of WAVE-1 causes sensorimotor retardation and reduced learning and memory in mice. 
  • Mapping of the mouse Rxr loci encoding nuclear retinoid X receptors RXR alpha, RXR beta, and RXR gamma. 
  • Meiotic recombination involving heterozygous large insertions in Saccharomyces cerevisiae: formation and repair of large, unpaired DNA loops. 
  • Methods for precise sizing, automated binning of alleles, and reduction of error rates in large-scale genotyping using fluorescently labeled dinucleotide markers. FUSION (Finland-U.S. Investigation of NIDDM Genetics) Study Group. 
  • Methylation of the FSHD syndrome-linked subtelomeric repeat in normal and FSHD cell cultures and tissues. 
  • Mice deficient for the vesicular acetylcholine transporter are myasthenic and have deficits in object and social recognition. 
  • Mitochondrial DNA structure and expression in specialized subtypes of mammalian striated muscle. 
  • Mitotic and meiotic gene conversion of Ty elements and other insertions in Saccharomyces cerevisiae. 
  • Mitotic recombination in the rDNA of S. cerevisiae is suppressed by the combined action of DNA topoisomerases I and II. 
  • Molecular analysis of P16(Ink4)/CDKN2 and P15(INK4B)/MTS2 genes in primary human testicular germ cell tumors. 
  • Molecular analysis of mutations in a patient with purine nucleoside phosphorylase deficiency. 
  • Molecular and immunochemical analyses of RB1 and cyclin D1 in human ductal pancreatic carcinomas and cell lines. 
  • Molecular basis for elliptocytosis associated with glycophorin C and D deficiency in the Leach phenotype. 
  • Molecular cloning and expression of the cDNA for the alpha 1A-adrenergic receptor. The gene for which is located on human chromosome 5. 
  • Molecular cloning of a cDNA of a camptothecin-resistant human DNA topoisomerase I and identification of mutation sites. 
  • Molecular cloning of the structural gene for exopolygalacturonate lyase from Erwinia chrysanthemi EC16 and characterization of the enzyme product. 
  • Molecular cloning, characterization, and expression in Escherichia coli of full-length cDNAs of three human glutathione S-transferase Pi gene variants. Evidence for differential catalytic activity of the encoded proteins. 
  • Molecular genetic and immunohistochemical analysis of the tumor suppressor genes Rb and p53 in palmar and aggressive fibromatosis. 
  • Molecular mechanism of tissue-specific regulation of mouse renin gene expression by cAMP. Identification of an inhibitory protein that binds nuclear transcriptional factor. 
  • Molecular structure of a protein-tyrosine/threonine kinase activating p42 mitogen-activated protein (MAP) kinase: MAP kinase kinase. 
  • Mosaicism for an FMR1 gene deletion in a fragile X female. 
  • Mouse embryonic germ (EG) cell lines: transmission through the germline and differences in the methylation imprint of insulin-like growth factor 2 receptor (Igf2r) gene compared with embryonic stem (ES) cell lines. 
  • Mouse small eye results from mutations in a paired-like homeobox-containing gene. 
  • Multiple DNA repair mechanisms and alkylator resistance in the human medulloblastoma cell line D-283 Med (4-HCR). 
  • Mutations of the p16 gene product are rare in prostate cancer. 
  • Myotilin is not the causative gene for vocal cord and pharyngeal weakness with distal myopathy (VCPDM). 
  • NF1-related locus on chromosome 15. 
  • No evidence for extrapair fertilizations in the merlin revealed by DNA fingerprinting. 
  • North, south, or east? Blotting techniques. 
  • On the ultrastructure of hyalin, a cell adhesion protein of the sea urchin embryo extracellular matrix. 
  • Oncogene amplification in pediatric brain tumors. 
  • Organization of ribosomal DNA in the basidiomycete Thanatephorus praticola. 
  • Organization of the human zeta-crystallin/quinone reductase gene (CRYZ). 
  • Overexpression of oncogenic STK15/BTAK/Aurora A kinase in human pancreatic cancer. 
  • Overexpression of the rat sarcoplasmic reticulum Ca2+ ATPase gene in the heart of transgenic mice accelerates calcium transients and cardiac relaxation. 
  • PIG-A, DAF and proto-oncogene expression in paroxysmal nocturnal haemoglobinuria-associated acute myelogenous leukaemia blasts. 
  • Paradoxical expression of adenosine deaminase in T cells cultured from a patient with adenosine deaminase deficiency and combine immunodeficiency. 
  • Partial correction of endogenous DeltaF508 CFTR in human cystic fibrosis airway epithelia by spliceosome-mediated RNA trans-splicing. 
  • Pleiotropic effects of deubiquitinating enzyme Ubp5 on growth and pathogenesis of Cryptococcus neoformans. 
  • Potential for germ line transmission after intramyocardial gene delivery by adeno-associated virus. 
  • Precise localization of the FHIT gene to the common fragile site at 3p14.2 (FRA3B) and characterization of homozygous deletions within FRA3B that affect FHIT transcription in tumor cell lines. 
  • Primary structure and domain organization of human alpha and beta adducin. 
  • Prolactin receptor gene expression in the fetal rat. 
  • RNase 1 genes from the family Sciuridae define a novel rodent ribonuclease cluster. 
  • Rapamycin antifungal action is mediated via conserved complexes with FKBP12 and TOR kinase homologs in Cryptococcus neoformans. 
  • Rapid telomere shortening in children. 
  • Recombination of 4p16 DNA markers in an unusual family with Huntington disease. 
  • Regulated expression of a vitellogenin fusion gene in transgenic nematodes. 
  • Regulation of T cell receptor delta gene rearrangement by c-Myb. 
  • Regulation of genome stability by TEL1 and MEC1, yeast homologs of the mammalian ATM and ATR genes. 
  • Repair analysis of 4-hydroperoxycyclophosphamide-induced DNA interstrand crosslinking in the c-myc gene in 4-hydroperoxycyclophosphamide-sensitive and -resistant medulloblastoma cell lines. 
  • Retina-specifically expressed novel subtypes of bovine cyclophilin. 
  • Rhabdoid tumour: a malignancy of early childhood with variable primary site, histology and clinical behaviour. 
  • Sequence length required for homologous recombination in Cryptococcus neoformans. 
  • Structure of the gene encoding the human B lymphocyte differentiation antigen CD20 (B1). 
  • Structure of the genes encoding the CD19 antigen of human and mouse B lymphocytes. 
  • Suppressor analysis of temperature-sensitive RNA polymerase I mutations in Saccharomyces cerevisiae: suppression of mutations in a zinc-binding motif by transposed mutant genes. 
  • Telomeres and the natural lifespan limit in humans. 
  • The GA2 locus of Arabidopsis thaliana encodes ent-kaurene synthase of gibberellin biosynthesis. 
  • The SATE pronucleotide approach applied to acyclovir: part II. Effects of bis(SATE)phosphotriester derivatives of acyclovir on duck hepatitis B virus replication in vitro and in vivo. 
  • The actin gene from Cryptococcus neoformans: structure and phylogenetic analysis. 
  • The aminopeptidase ERAAP shapes the peptide repertoire displayed by major histocompatibility complex class I molecules. 
  • The anti-La response of a single MRL/Mp-lpr/lpr mouse: specificity for DNA and VH gene usage. 
  • The expression of platelet-derived growth-factor gene in Dupuytren contracture. 
  • The gene encoding the glutamate receptor subunit GluR5 is located on human chromosome 21q21.1-22.1 in the vicinity of the gene for familial amyotrophic lateral sclerosis. 
  • The human plasminogen activator inhibitor type I gene promoter targets to kidney. 
  • The isolation and characterization of a novel cDNA demonstrating an altered mRNA level in nontumorigenic Wilms' microcell hybrid cells. 
  • The isolation and characterization of cDNA encoding the mouse bifunctional ATP sulfurylase-adenosine 5'-phosphosulfate kinase. 
  • The mammalian twisted gastrulation gene functions in foregut and craniofacial development. 
  • The mechanism of autoantibody production in an autoimmune MRL/lpr mouse. 
  • The mouse Cd83 gene: structure, domain organization, and chromosome localization. 
  • The origin of O6-methylguanine-DNA methyltransferase in Chinese hamster ovary cells transfected with human DNA. 
  • The regulatory light chain of nonmuscle myosin is encoded by spaghetti-squash, a gene required for cytokinesis in Drosophila. 
  • Tissue-specific expression in mammalian brain, heart, and muscle of S1, a member of the elongation factor-1 alpha gene family. 
  • Tissue-specific expression of the rat androgen-binding protein/sex hormone-binding globulin gene in transgenic mice. 
  • Transcriptional network of multiple capsule and melanin genes governed by the Cryptococcus neoformans cyclic AMP cascade. 
  • Transforming growth factor-beta-mediated p15(INK4B) induction and growth inhibition in astrocytes is SMAD3-dependent and a pathway prominently altered in human glioma cell lines. 
  • Two cyclophilin A homologs with shared and distinct functions important for growth and virulence of Cryptococcus neoformans. 
  • Two tRNA gene clusters associated with rRNA operons rrnD and rrnE in Bacillus subtilis. 
  • Type 1 neurofibromatosis gene: correction. 
  • Type 1 neurofibromatosis gene: identification of a large transcript disrupted in three NF1 patients. 
  • Tyrosine kinase activity and transformation potency of bcr-abl oncogene products. 
  • U19/Eaf2 knockout causes lung adenocarcinoma, B-cell lymphoma, hepatocellular carcinoma and prostatic intraepithelial neoplasia. 
  • X-linked high myopia associated with cone dysfunction. 
  • Y353/B: a candidate multiple-copy spermiogenesis gene on the mouse Y chromosome. 
  • cDNA cloning and chromosomal localization of the human beta-adrenergic receptor kinase. 
  • cDNA sequence and genomic structure of EV12B, a gene lying within an intron of the neurofibromatosis type 1 gene. 
  • n-Butyrate, a cell cycle blocker, inhibits early amplification of duck hepatitis B virus covalently closed circular DNA after in vitro infection of duck hepatocytes. 
• 

  Keywords of People

  • Bennett, Vann, George Barth Geller Distinguished Professor of Molecular Biology, Duke Cancer Institute
  • Berchuck, Andrew, James M. Ingram Distinguished Professor of Gynecologic Oncology, Obstetrics and Gynecology, Gynecologic Oncology
  • Ferreira, Paulo Alexandre, Associate Professor in Ophthalmology, Pathology
  • Freemark, Michael Scott, Robert C. Atkins, M.D. and Veronica Atkins Distinguished Professor of Pediatrics, in the School of Medicine, Pediatrics, Endocrinology
  • Krangel, Michael S., George Barth Geller Distinguished Professor of Immunology, Integrative Immunobiology
  • Soderling, Scott Haydn, George Barth Geller Distinguished Professor of Molecular Biology, Cell Biology
  • Tsalik, Ephraim, Adjunct Associate Professor in the Department of Medicine, Medicine, Infectious Diseases