-
Subject Areas on Research
-
De Novo
Design, Solution Characterization, and Crystallographic Structure of an Abiological Mn-Porphyrin-Binding Protein Capable of Stabilizing a Mn(V) Species.
-
A fluorogenic C. neoformans reporter strain with a robust expression of m-cherry expressed from a safe haven site in the genome.
-
A glycoengineered anti-CD19 antibody with potent antibody-dependent cellular cytotoxicity activity in vitro and lymphoma growth inhibition in vivo.
-
A highly parallel method for synthesizing DNA repeats enables the discovery of 'smart' protein polymers.
-
A light-inducible CRISPR-Cas9 system for control of endogenous gene activation.
-
A miniaturized technique for assessing protein thermodynamics and function using fast determination of quantitative cysteine reactivity.
-
A nanoscale force probe for gauging intermolecular interactions.
-
A rationally-designed chimeric KDM1A/KDM1B histone demethylase tower domain deletion mutant retaining enzymatic activity.
-
A unified model for de novo design of elastin-like polypeptides with tunable inverse transition temperatures.
-
Addressing cell-sourcing limitations with gene therapy.
-
Algorithm for backrub motions in protein design.
-
Allosteric cooperation in a de novo-designed two-domain protein.
-
Altering the Immunogenicity of Hemagglutinin Immunogens by Hyperglycosylation and Disulfide Stabilization.
-
An engineered liver glycogen phosphorylase with AMP allosteric activation.
-
An engineered vascular endothelial growth factor-activating transcription factor induces therapeutic angiogenesis in ApoE knockout mice with hindlimb ischemia.
-
Analysis of allosteric signal transduction mechanisms in an engineered fluorescent maltose biosensor.
-
Analysis of the structural properties of cAMP-responsive element-binding protein (CREB) and phosphorylated CREB.
-
Antibody light-chain-restricted recognition of the site of immune pressure in the RV144 HIV-1 vaccine trial is phylogenetically conserved.
-
Atomic force microscopy captures length phenotypes in single proteins.
-
B-cell depletion in vitro and in vivo with an afucosylated anti-CD19 antibody.
-
Betadoublet: de novo design, synthesis, and characterization of a beta-sandwich protein.
-
BioWarehouse: a bioinformatics database warehouse toolkit.
-
Bioinformatic design of A-kinase anchoring protein-in silico: a potent and selective peptide antagonist of type II protein kinase A anchoring.
-
C-terminal opening mimics 'inside-out' activation of integrin alpha5beta1.
-
CD86 (B7-2) can function to drive MHC-restricted antigen-specific CTL responses in vivo.
-
Cell-Specific Chemical Delivery Using a Selective Nitroreductase-Nitroaryl Pair.
-
Coming together at the hinges: Therapeutic prospects of IgG3.
-
Comparative immunogenicity of HIV-1 gp160, gp140 and gp120 expressed by live attenuated newcastle disease virus vector.
-
Computation-guided backbone grafting of a discontinuous motif onto a protein scaffold.
-
Computational design of a biologically active enzyme.
-
Computational design of high-affinity epitope scaffolds by backbone grafting of a linear epitope.
-
Computational design of protein antigens that interact with the CDR H3 loop of HIV broadly neutralizing antibody 2F5.
-
Computational protein engineering.
-
Conceptual Approaches to Modulating Antibody Effector Functions and Circulation Half-Life.
-
Construction and phenotypic characterization of HIV type 1 functional envelope clones of subtypes G and F.
-
Construction of a catalytically active iron superoxide dismutase by rational protein design.
-
Construction of a family of Cys2His2 zinc binding sites in the hydrophobic core of thioredoxin by structure-based design.
-
Construction of a novel redox protein by rational design: conversion of a disulfide bridge into a mononuclear iron-sulfur center.
-
Construction of new ligand binding sites in proteins of known structure. I. Computer-aided modeling of sites with pre-defined geometry.
-
Construction of new ligand binding sites in proteins of known structure. II. Grafting of a buried transition metal binding site into Escherichia coli thioredoxin.
-
Conversion of a maltose receptor into a zinc biosensor by computational design.
-
Converting a maltose receptor into a nascent binuclear copper oxygenase by computational design.
-
De novo design of covalently constrained mesosize protein scaffolds with unique tertiary structures.
-
Design of a calcium-binding protein with desired structure in a cell adhesion molecule.
-
Design of bioelectronic interfaces by exploiting hinge-bending motions in proteins.
-
Design, expression, and immunogenicity of a soluble HIV trimeric envelope fragment adopting a prefusion gp41 configuration.
-
Designing an extracellular matrix protein with enhanced mechanical stability.
-
Development of DNA Vaccine Targeting E6 and E7 Proteins of Human Papillomavirus 16 (HPV16) and HPV18 for Immunotherapy in Combination with Recombinant Vaccinia Boost and PD-1 Antibody.
-
Digital switching of local arginine density in a genetically encoded self-assembled polypeptide nanoparticle controls cellular uptake.
-
Direct force measurements of the streptavidin-biotin interaction.
-
Direct observation of multimer stabilization in the mechanical unfolding pathway of a protein undergoing oligomerization.
-
Directed evolution of a pyruvate aldolase to recognize a long chain acyl substrate.
-
Effect of genetic circular permutation near the active site on the activity and stability of an enzyme inhibitor.
-
Effect of protein fusion on the transition temperature of an environmentally responsive elastin-like polypeptide: a role for surface hydrophobicity?
-
Elastin-Like Polypeptides for Biomedical Applications.
-
Eliminating antibody polyreactivity through addition of N-linked glycosylation.
-
Engineered Ribonucleoprotein Granules Inhibit Translation in Protocells.
-
Engineered chimeric streptavidin tetramers as novel tools for bioseparations and drug delivery.
-
Engineered leucine zippers show that hemiphosphorylated CREB complexes are transcriptionally active.
-
Engineered zinc finger-activating vascular endothelial growth factor transcription factor plasmid DNA induces therapeutic angiogenesis in rabbits with hindlimb ischemia.
-
Engineering a BCR-ABL-activated caspase for the selective elimination of leukemic cells.
-
Engineering oxidative stability in human hemoglobin based on the Hb providence (βK82D) mutation and genetic cross-linking.
-
Engineering protein stability and specificity using fluorous amino acids: the importance of packing effects.
-
Engineering the substrate specificity of Staphylococcus aureus Sortase A. The beta6/beta7 loop from SrtB confers NPQTN recognition to SrtA.
-
Engineering, expression, purification, and characterization of stable clade A/B recombinant soluble heterotrimeric gp140 proteins.
-
Enhanced MyoD-induced transdifferentiation to a myogenic lineage by fusion to a potent transactivation domain.
-
Enzyme redesign guided by cancer-derived IDH1 mutations.
-
Epitope tagging for tracking elastin-like polypeptides.
-
Facilitative glucose transporter Glut1 is actively excluded from rod outer segments.
-
Found in translation: applications of protein and peptide molecular diversity.
-
Founder's Award, Society for Biomaterials. Sixth World Biomaterials Congress 2000, Kamuela, HI,May 15-20, 2000. Really smart bioconjugates of smart polymers and receptor proteins.
-
Gene targeting to the ROSA26 locus directed by engineered zinc finger nucleases
-
Generalized dead-end elimination algorithms make large-scale protein side-chain structure prediction tractable: implications for protein design and structural genomics.
-
Generation of high-affinity human antibodies by combining donor-derived and synthetic complementarity-determining-region diversity.
-
Generation, characterization and crystallization of a highly active and stable cytochrome bc1 complex mutant from Rhodobacter sphaeroides.
-
Genetic engineering of a single-chain antibody fragment for surface immobilization in an optical biosensor.
-
Genetic engineering of a suboptimal islet graft with A20 preserves beta cell mass and function.
-
Genetically Encoded Cholesterol-Modified Polypeptides.
-
HIV-1 VACCINES. HIV-1 neutralizing antibodies induced by native-like envelope trimers.
-
Highly active zinc-finger nucleases by extended modular assembly.
-
Human interleukin-2 production in insect (Trichoplusia ni) larvae: effects and partial control of proteolysis.
-
Identification of cognate ligands for the Escherichia coli phnD protein product and engineering of a reagentless fluorescent biosensor for phosphonates.
-
In mice with type 2 diabetes, a vascular endothelial growth factor (VEGF)-activating transcription factor modulates VEGF signaling and induces therapeutic angiogenesis after hindlimb ischemia.
-
In situ growth of a PEG-like polymer from the C terminus of an intein fusion protein improves pharmacokinetics and tumor accumulation.
-
Incorporation of low molecular weight molecules into alpha(2)-macroglobulin by nucleophilic exchange.
-
Integration of cell-free protein coexpression with an enzyme-linked immunosorbent assay enables rapid analysis of protein-protein interactions directly from DNA.
-
Light-inducible gene regulation with engineered zinc finger proteins.
-
Light-inducible spatiotemporal control of gene activation by customizable zinc finger transcription factors.
-
Local encoding of computationally designed enzyme activity.
-
Manipulation of ligand binding affinity by exploitation of conformational coupling.
-
Mechanical and chemical unfolding of a single protein: a comparison.
-
Mechanical design of proteins studied by single-molecule force spectroscopy and protein engineering.
-
Metalloprotein design.
-
Molecular biology in cardiology: recent developments and opportunities for clinical applications.
-
Molecular engineering of proteins and polymers for targeting and intracellular delivery of therapeutics.
-
Monoclonal antibody therapy of human gliomas: current status and future approaches.
-
Multidimensional Tracking of GPCR Signaling via Peroxidase-Catalyzed Proximity Labeling.
-
Multiplex-PCR-based recombination as a novel high-fidelity method for directed evolution.
-
Mutagenesis of the phosphate-binding pocket of KDPG aldolase enhances selectivity for hydrophobic substrates.
-
Nanomechanics of streptavidin hubs for molecular materials.
-
Natural product glycosyltransferases: properties and applications.
-
Neural circuits. Labeling of active neural circuits in vivo with designed calcium integrators.
-
Neutralization-guided design of HIV-1 envelope trimers with high affinity for the unmutated common ancestor of CH235 lineage CD4bs broadly neutralizing antibodies.
-
Novel disulfide engineering in human carbonic anhydrase II using the PAIRWISE side-chain geometry database.
-
Optogenetic photochemical control of designer K+ channels in mammalian neurons.
-
Orthogonal site-specific protein modification by engineering reversible thiol protection mechanisms.
-
Overcoming the solubility limit with solubility-enhancement tags: successful applications in biomolecular NMR studies.
-
Periplasmic binding proteins: a versatile superfamily for protein engineering.
-
Plug-and-Play Protein Modification Using Homology-Independent Universal Genome Engineering.
-
Production of adrenergic receptors in yeast.
-
Protein engineering and the development of generic biosensors.
-
Protein engineering approaches to biomaterials design.
-
Protein fabrication automation.
-
Psychedelic-inspired drug discovery using an engineered biosensor.
-
Quantitation of protein-protein interactions by thermal stability shift analysis.
-
RNA-guided gene activation by CRISPR-Cas9-based transcription factors.
-
Rapid generation of potent antibodies by autonomous hypermutation in yeast.
-
Rational design and generation of recombinant control reagents for bispecific antibodies through CDR mutagenesis.
-
Rational design of a calcium-binding protein.
-
Rational protein design: combining theory and experiment.
-
Re-design of Rhodobacter sphaeroides dimethyl sulfoxide reductase. Enhancement of adenosine N1-oxide reductase activity.
-
Re-engineered CD40 receptor enables potent pharmacological activation of dendritic-cell cancer vaccines in vivo.
-
Red cell antigens as functional molecules and obstacles to transfusion.
-
Reverse engineering of the giant muscle protein titin.
-
Role of citrate and phosphate anions in the mechanism of iron(III) sequestration by ferric binding protein: kinetic studies of the formation of the holoprotein of wild-type FbpA and its engineered mutants.
-
Runx2/Cbfa1-genetically engineered skeletal myoblasts mineralize collagen scaffolds in vitro.
-
Single protein misfolding events captured by atomic force microscopy.
-
Site-specific conjugation of a temperature-sensitive polymer to a genetically-engineered protein.
-
Small near-infrared photochromic protein for photoacoustic multi-contrast imaging and detection of protein interactions in vivo.
-
Special issue on engineered DNA-binding proteins.
-
Streptavidin-biotin binding energetics.
-
Structural optimization of a "smart" doxorubicin-polypeptide conjugate for thermally targeted delivery to solid tumors.
-
Structure-based redesign of corepressor specificity of the Escherichia coli purine repressor by substitution of residue 190.
-
Structure-guided reprogramming of serine recombinase DNA sequence specificity.
-
SymROP: ROP protein with identical helices redesigned by all-atom contact analysis and molecular dynamics.
-
Synthesis and characterization of a ruthenium(II)-based redox conjugate for reagentless biosensing.
-
Synthesis of programmable integrases.
-
Targeted entry via somatostatin receptors using a novel modified retrovirus glycoprotein that delivers genes at levels comparable to those of wild-type viral glycoproteins.
-
Targeted intracellular degradation of SARS-CoV-2 via computationally optimized peptide fusions.
-
Targeted plasmid integration into the human genome by an engineered zinc-finger recombinase.
-
Targeting a genetically engineered elastin-like polypeptide to solid tumors by local hyperthermia.
-
Temperature sensitive peptides: engineering hyperthermia-directed therapeutics.
-
The Chimpanzee SIV Envelope Trimer: Structure and Deployment as an HIV Vaccine Template.
-
Three amino acid substitutions in domain I of calmodulin prevent the activation of chicken smooth muscle myosin light chain kinase.
-
Visualization of synaptic inhibition with an optogenetic sensor developed by cell-free protein engineering automation.
-
X-ray crystallographic studies of streptavidin mutants binding to biotin.
-
Keywords of People
-
-
Bennett, Vann,
George Barth Geller Distinguished Professor of Molecular Biology,
Duke Cancer Institute
-
Erickson, Harold Paul,
Professor Emeritus of Cell Biology,
Cell Biology
-
Hoffman, Brenton D.,
James L. and Elizabeth M. Vincent Associate Professor of Biomedical Engineering,
Biomedical Engineering
-
Reddy, Timothy E,
Associate Professor of Biostatistics & Bioinformatics,,
Molecular Genetics and Microbiology
-
Richardson, David C.,
Professor of Biochemistry,
Biochemistry
-
Sampson, John Howard,
Robert H., M.D. and Gloria Wilkins Professor of Neurosurgery, in the School of Medicine,
Biomedical Engineering
-
Soderling, Scott Haydn,
George Barth Geller Distinguished Professor of Molecular Biology,
Cell Biology
-
Yan, Hai,
Adjunct Professor of Pathology,
Pathology