Transducin

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  Subject Areas on Research

  • A specific domain of Gialpha required for the transactivation of Gialpha by tubulin is implicated in the organization of cellular microtubules. 
  • An effector site that stimulates G-protein GTPase in photoreceptors. 
  • Analysis of the molecular interaction of the farnesyl moiety of transducin through the use of a photoreactive farnesyl analogue. 
  • Arrestin translocation is induced at a critical threshold of visual signaling and is superstoichiometric to bleached rhodopsin. 
  • CAMP-mediated inhibition of the renal brush border membrane Na+-H+ exchanger requires a dissociable phosphoprotein cofactor. 
  • Co-expression of nAChRs and molecules of the bitter taste transduction pathway by epithelial cells of intrapulmonary airways. 
  • Dimerization deficiency of enigmatic retinitis pigmentosa-linked rhodopsin mutants. 
  • Electrostatic and lipid anchor contributions to the interaction of transducin with membranes: mechanistic implications for activation and translocation. 
  • Enzymology of GTPase acceleration in phototransduction. 
  • Fluorescent probes as indicators of conformation changes in transducin on activation. 
  • Functional comparison of RGS9 splice isoforms in a living cell. 
  • Functional differences in the beta gamma complexes of transducin and the inhibitory guanine nucleotide regulatory protein. 
  • Functional reconstitution of the alpha 2-adrenergic receptor with guanine nucleotide regulatory proteins in phospholipid vesicles. 
  • Functional roles of the two domains of phosducin and phosducin-like protein. 
  • Functionally rodless mice: transgenic models for the investigation of cone function in retinal disease and therapy. 
  • G alpha t/G alpha i1 chimeras used to define structural basis of specific functions of G alpha t. 
  • G proteins and phototransduction. 
  • Growth factor receptor-bound protein 14 undergoes light-dependent intracellular translocation in rod photoreceptors: functional role in retinal insulin receptor activation. 
  • Histologic development of the human fovea from midgestation to maturity. 
  • Increased proteasomal activity supports photoreceptor survival in inherited retinal degeneration. 
  • Interacting targets of the farnesyl of transducin gamma-subunit. 
  • Interaction sites of the C-terminal region of the cGMP phosphodiesterase inhibitory subunit with the GDP-bound transducin alpha-subunit. 
  • Interaction sites of the COOH-terminal region of the gamma subunit of cGMP phosphodiesterase with the GTP-bound alpha subunit of transducin. 
  • Kinetic approaches to study the function of RGS9 isoforms. 
  • Mapping of effector binding sites of transducin alpha-subunit using G alpha t/G alpha i1 chimeras. 
  • Massive light-driven translocation of transducin between the two major compartments of rod cells: a novel mechanism of light adaptation. 
  • Mechanism of guanine nucleotide regulatory protein-mediated inhibition of adenylate cyclase. Studies with isolated subunits of transducin in a reconstituted system. 
  • Mechanisms regulating variability of the single photon responses of mammalian rod photoreceptors. 
  • Mechanistic basis for the failure of cone transducin to translocate: why cones are never blinded by light. 
  • Molecular basis for interactions of G protein betagamma subunits with effectors. 
  • Multiple alpha subunits of guanine nucleotide-binding proteins in Dictyostelium. 
  • Negative regulation of TSHalpha target gene by thyroid hormone involves histone acetylation and corepressor complex dissociation. 
  • Noncatalytic domains of RGS9-1.Gbeta 5L play a decisive role in establishing its substrate specificity. 
  • On the role of transducin GTPase in the quenching of a phosphodiesterase cascade of vision. 
  • Onset of feedback reactions underlying vertebrate rod photoreceptor light adaptation. 
  • Opsin, G-protein and 48-kDa protein in normal and rd mouse retinas: developmental expression of mRNAs and proteins and light/dark cycling of mRNAs. 
  • Phosducin facilitates light-driven transducin translocation in rod photoreceptors. Evidence from the phosducin knockout mouse. 
  • Phosducin regulates the expression of transducin betagamma subunits in rod photoreceptors and does not contribute to phototransduction adaptation. 
  • Phosphorylation of G protein-coupled receptor kinase 1 (GRK1) is regulated by light but independent of phototransduction in rod photoreceptors. 
  • Photoreceptor-specific nuclear receptor NR2E3 functions as a transcriptional activator in rod photoreceptors. 
  • Proteasome overload is a common stress factor in multiple forms of inherited retinal degeneration. 
  • RGS9-G beta 5 substrate selectivity in photoreceptors. Opposing effects of constituent domains yield high affinity of RGS interaction with the G protein-effector complex. 
  • Regulation of deactivation of photoreceptor G protein by its target enzyme and cGMP. 
  • Regulation of transducin GTPase activity in bovine rod outer segments. 
  • Role for the target enzyme in deactivation of photoreceptor G protein in vivo. 
  • Specificity of the functional interactions of the beta-adrenergic receptor and rhodopsin with guanine nucleotide regulatory proteins reconstituted in phospholipid vesicles. 
  • Structural requirements of the photoreceptor phosphodiesterase gamma-subunit for inhibition of rod PDE6 holoenzyme and for its activation by transducin. 
  • The GTPase activating factor for transducin in rod photoreceptors is the complex between RGS9 and type 5 G protein beta subunit. 
  • The N terminus of GTP gamma S-activated transducin alpha-subunit interacts with the C terminus of the cGMP phosphodiesterase gamma-subunit. 
  • The alpha-helical domain of Galphat determines specific interaction with regulator of G protein signaling 9. 
  • The carboxyl terminus of the gamma-subunit of rod cGMP phosphodiesterase contains distinct sites of interaction with the enzyme catalytic subunits and the alpha-subunit of transducin. 
  • The effector enzyme regulates the duration of G protein signaling in vertebrate photoreceptors by increasing the affinity between transducin and RGS protein. 
  • The gain of rod phototransduction: reconciliation of biochemical and electrophysiological measurements. 
  • The male-specific region of the human Y chromosome is a mosaic of discrete sequence classes. 
  • Transducin GTPase provides for rapid quenching of the cGMP cascade in rod outer segments. 
  • Transducin and the inhibitory nucleotide regulatory protein inhibit the stimulatory nucleotide regulatory protein mediated stimulation of adenylate cyclase in phospholipid vesicle systems. 
  • Transducin gamma-subunit sets expression levels of alpha- and beta-subunits and is crucial for rod viability. 
  • Transducin translocation in rods is triggered by saturation of the GTPase-activating complex. 
  • Transducin β-Subunit Can Interact with Multiple G-Protein γ-Subunits to Enable Light Detection by Rod Photoreceptors. 
  • Ubiquitylation of the transducin betagamma subunit complex. Regulation by phosducin. 
  • cGMP binding sites on photoreceptor phosphodiesterase: role in feedback regulation of visual transduction. 
  • cGMP suppresses GTPase activity of a portion of transducin equimolar to phosphodiesterase in frog rod outer segments. Light-induced cGMP decreases as a putative feedback mechanism of the photoresponse. 
  • mRNAs coding for proteins of the cGMP cascade in the degenerative retina of the rd mouse. 
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  Keywords of People

  • Pitt, Geoffrey Stuart, Adjunct Professor in the Department of Medicine, Medicine, Cardiology