Oligonucleotide Probes

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  Subject Areas on Research

  • A distinct wave of human T cell receptor gamma/delta lymphocytes in the early fetal thymus: evidence for controlled gene rearrangement and cytokine production. 
  • A molecular mechanism for the differential regulation of TGF-beta1 expression due to the common SNP -509C-T (c. -1347C > T). 
  • A naturally occurring gamma globin gene mutation enhances SP1 binding activity. 
  • A new strategy for studying protein kinase B and its three isoforms. Role of protein kinase B in phosphorylating glycogen synthase kinase-3, tuberin, WNK1, and ATP citrate lyase. 
  • A novel Ca2+/calmodulin-dependent protein kinase and a male germ cell-specific calmodulin-binding protein are derived from the same gene. 
  • A novel species-specific RNA related to alternatively spliced amyloid precursor protein mRNAs. 
  • A pathway-based classification of human breast cancer. 
  • Absence of mutation in the beta-amyloid cDNAs cloned from the brains of three patients with sporadic Alzheimer's disease. 
  • Absent ras gene mutations in human adrenal cortical neoplasms and pheochromocytomas. 
  • Alternative processing of androgen-binding protein RNA transcripts in fetal rat liver. Identification of a transcript formed by trans splicing. 
  • Amplification of HIV-1 provirus from cerebrospinal fluid and its correlation with neurologic disease. 
  • Analysis of HLA-DM polymorphism in juvenile dermatomyositis (JDM) patients. 
  • Analysis of mRNA partitioning between the cytosol and endoplasmic reticulum compartments of mammalian cells. 
  • AnkyrinG is required for clustering of voltage-gated Na channels at axon initial segments and for normal action potential firing. 
  • Anti-DNA antibodies from autoimmune mice arise by clonal expansion and somatic mutation. 
  • Bidirectional excision in methyl-directed mismatch repair. 
  • Calcium/calmodulin-dependent protein kinase types II and IV differentially regulate CREB-dependent gene expression. 
  • Characterization of a protein cofactor that mediates protein kinase A regulation of the renal brush border membrane Na(+)-H+ exchanger. 
  • Characterization of four novel ras-like genes expressed in a human teratocarcinoma cell line. 
  • Cloning of human and mouse brain cDNAs coding for S1, the second member of the mammalian elongation factor-1 alpha gene family: analysis of a possible evolutionary pathway. 
  • Conservation of the D-mannose-adhesion protein among type 1 fimbriated members of the family Enterobacteriaceae. 
  • Creation of an active estrogen-responsive element by a single base change in the flanking sequence of a cellular oncogene: a possible mechanism for hormonal carcinogenesis? 
  • Designer microarrays: from soup to nuts. 
  • Detection of mouse cytomegalovirus nucleic acid in latently infected mice by in vitro enzymatic amplification. 
  • Detection of phylogenetically diverse human immunodeficiency virus type 1 groups M and O from plasma by using highly sensitive and specific generic primers. 
  • Detection of sequences homologous to human retroviral DNA in multiple sclerosis by gene amplification. 
  • Determination of estrogen receptor messenger ribonucleic acid (mRNA) and cytochrome P450 aromatase mRNA levels in adipocytes and adipose stromal cells by competitive polymerase chain reaction amplification. 
  • Differential expression and regulation of the glucokinase gene in liver and islets of Langerhans. 
  • Differential expression of gamma-aminobutyric acidA receptor subunits. 
  • Differential expression of immediate early genes in the hippocampus in the kindling model of epilepsy. 
  • Disruption of PAX6 function in mice homozygous for the Pax6Sey-1Neu mutation produces abnormalities in the early development and regionalization of the diencephalon. 
  • Do clinical manifestations of resistance to thyroid hormone correlate with the functional alteration of the corresponding mutant thyroid hormone-beta receptors? 
  • Epidermal growth factor replaces estrogen in the stimulation of female genital-tract growth and differentiation. 
  • Evaluation of the accuracy and reproducibility of a practical PCR panel assay for rapid detection and differentiation of Mycobacterium avium subspecies. 
  • Expansion of the alpha 2-adrenergic receptor family: cloning and characterization of a human alpha 2-adrenergic receptor subtype, the gene for which is located on chromosome 2. 
  • Expression of transforming growth factor-beta type II receptor in rat lung is regulated during development. 
  • FK 506-binding protein proline rotamase is a target for the immunosuppressive agent FK 506 in Saccharomyces cerevisiae. 
  • Genetic basis for p53 overexpression in human breast cancer. 
  • H19, a developmentally regulated gene, is reexpressed in rat vascular smooth muscle cells after injury. 
  • HNF-3 beta as a regulator of floor plate development. 
  • Human papillomavirus types 16 and 18 are not involved in human prostate carcinogenesis: analysis of archival human prostate cancer specimens by differential polymerase chain reaction. 
  • Human papillomavirus types important in progression of inverted papilloma. 
  • Identification of a cleavage site directing the immunochemical detection of molecular abnormalities in type IIA von Willebrand factor. 
  • Identification of the ral and rac1 gene products, low molecular mass GTP-binding proteins from human platelets. 
  • Increased expression of galanin in the rat superior cervical ganglion after pre- and postganglionic nerve lesions. 
  • Induction of a human carbonyl reductase gene located on chromosome 21. 
  • Induction of cyclooxygenase-2 in human endometrial stromal cells by malignant endometrial epithelial cells: evidence for the involvement of extracellularly regulated kinases and CCAAT/enhancer binding proteins. 
  • Isolation and heterologous expression of a cDNA encoding bovine inositol polyphosphate 1-phosphatase. 
  • K+ regulates DNA binding of transcription factors to control gene expression related to neuronal apoptosis. 
  • Kinetics of expression of multiply spliced RNA in early human immunodeficiency virus type 1 infection of lymphocytes and monocytes. 
  • Kupffer cell depletion by gadolinium chloride enhances liver regeneration after partial hepatectomy in rats. 
  • MGSA/GRO transcription is differentially regulated in normal retinal pigment epithelial and melanoma cells. 
  • Maintenance of p53 alterations throughout breast cancer progression. 
  • Microarray analysis of gene expression in human donor sclera. 
  • Mismatch-, MutS-, MutL-, and helicase II-dependent unwinding from the single-strand break of an incised heteroduplex. 
  • Mitochondrial DNA structure and expression in specialized subtypes of mammalian striated muscle. 
  • Mitochondrial biogenesis in striated muscles: rapid induction of citrate synthase mRNA by nerve stimulation. 
  • Modulation of glutathione peroxidase expression by selenium: effect on human MCF-7 breast cancer cell transfectants expressing a cellular glutathione peroxidase cDNA and doxorubicin-resistant MCF-7 cells. 
  • Modulation of oncogene and tumor suppressor gene expression in a hamster model of chronic lung injury with varying degrees of pulmonary neuroendocrine cell hyperplasia. 
  • Molecular cloning and expression of cDNA encoding a peripheral-type benzodiazepine receptor. 
  • Molecular cloning of protein phosphatase inhibitor-1 and its expression in rat and rabbit tissues. 
  • Molecular sentinel-on-chip for SERS-based biosensing. 
  • Multiple proteins interact with the nuclear inhibitory protein repressor element in the human interleukin-3 promoter. 
  • Mutations of the Ki-ras oncogene in endometrial carcinoma. 
  • Myocyte nuclear factor, a novel winged-helix transcription factor under both developmental and neural regulation in striated myocytes. 
  • NPY and galanin in a hibernator: hypothalamic gene expression and effects on feeding. 
  • Neisseria gonorrhoeae FitA interacts with FitB to bind DNA through its ribbon-helix-helix motif. 
  • Nuclear responses to depletion of mitochondrial DNA in human cells. 
  • Overexpression of TAR sequences renders cells resistant to human immunodeficiency virus replication. 
  • Paradoxical expression of adenosine deaminase in T cells cultured from a patient with adenosine deaminase deficiency and combine immunodeficiency. 
  • Plasticity in the adult human oligodendrocyte lineage. 
  • Positive and negative elements regulate human interleukin 3 expression. 
  • Preliminary study using species-specific oligonucleotide probe for rRNA of Bilophila wadsworthia. 
  • Probe directed at a segment of Rickettsia rickettsii rRNA amplified with polymerase chain reaction. 
  • Production and role of cytokines in the CNS of mice with acute viral encephalomyelitis. 
  • Quantitative determination that one of two potential RNA-binding domains of the A protein component of the U1 small nuclear ribonucleoprotein complex binds with high affinity to stem-loop II of U1 RNA. 
  • RNA subunit of mitochondrial RNA-processing enzyme is induced by contractile activity in striated muscle. 
  • Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. 
  • Region-specific anti-thyroid hormone receptor (TR) antibodies detect changes in TR structure due to ligand-binding and dimerization. 
  • Regulation of gonadotropin-releasing hormone by protein kinase-A and -C in immortalized hypothalamic neurons. 
  • Retinoic acid modulation of alpha(1-->2) fucosyltransferase activity and sensitivity of tumor cells to LAK-mediated cytotoxicity. 
  • Screening for the breast cancer gene (BRCA1) using a biochip system and molecular beacon probes immobilized on solid surfaces. 
  • Species-specific oligonucleotide probes for rRNA of Clostridium difficile and related species. 
  • Studies of the regulatory mechanism of Ca2+/calmodulin-dependent protein kinase II. Mutation of threonine 286 to alanine and aspartate. 
  • Syntaxin: a synaptic protein implicated in docking of synaptic vesicles at presynaptic active zones. 
  • TGF-beta regulates expression of tenascin alternative-splicing isoforms in fetal rat lung. 
  • The Drosophila Tis11 protein and its effects on mRNA expression in flies. 
  • The NF1 locus encodes a protein functionally related to mammalian GAP and yeast IRA proteins. 
  • The arrangement of the immunoglobulin-like domains of ICAM-1 and the binding sites for LFA-1 and rhinovirus. 
  • The carboxyl-terminal repeat domain of RNA polymerase II is not required for transcription factor Sp1 to function in vitro. 
  • The potassium channel MBK1 (Kv1.1) is expressed in the mouse retina. 
  • Trans-activating rev protein of the human immunodeficiency virus 1 interacts directly and specifically with its target RNA. 
  • Transcription of orthopoxvirus telomeres at late times during infection. 
  • Tumor necrosis factor-alpha induces c-jun during the regenerative response to liver injury. 
  • Vaccinia virus directs the synthesis of early mRNAs containing 5' poly(A) sequences. 
  • Vitamin D receptor interaction with specific DNA requires a nuclear protein and 1,25-dihydroxyvitamin D3. 
  • Vitamin K-dependent protein S in Leydig cells of human testis. 
  • Whole genome genetic-typing in yeast using high-density oligonucleotide arrays. 
  • rac, a novel ras-related family of proteins that are botulinum toxin substrates. 
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  Keywords of People

  • Berchuck, Andrew, James M. Ingram Distinguished Professor of Gynecologic Oncology, Obstetrics and Gynecology, Gynecologic Oncology
  • Choi, Steven Sok, Associate Professor of Medicine, Medicine, Gastroenterology
  • Erickson, Harold Paul, James B. Duke Distinguished Professor Emeritus, Cell Biology
  • Hogan, Brigid L. M., Research Professor of Cell Biology, Cell Biology