Biosensing Techniques

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  Subject Areas on Research

  • "Smart" DNA interfaces. 
  • A Self-Calibrating Radar Sensor System for Measuring Vital Signs. 
  • A beta-arrestin/green fluorescent protein biosensor for detecting G protein-coupled receptor activation. 
  • A carrageenan hydrogel stabilized colloidal gold multi-enzyme biosensor electrode utilizing immobilized horseradish peroxidase and cholesterol oxidase/cholesterol esterase to detect cholesterol in serum and whole blood. 
  • A colorimetric gold nanoparticle sensor to interrogate biomolecular interactions in real time on a surface. 
  • A cut above the other caspases. 
  • A facile approach to immobilize protein for biosensor: self-assembled supported bilayer lipid membranes on glassy carbon electrode. 
  • A whole blood immunoassay using gold nanoshells. 
  • Acoustofluidic multimodal diagnostic system for Alzheimer's disease. 
  • Acquiring local field potential information from amperometric neurochemical recordings. 
  • Allosteric modulation of β-arrestin-biased angiotensin II type 1 receptor signaling by membrane stretch. 
  • Amperometric glucose biosensor based on lipid film. 
  • Amplification of antigen-antibody interactions based on biotin labeled protein-streptavidin network complex using impedance spectroscopy. 
  • An Engineered Metal Sensor Tunes the Kinetics of Synaptic Transmission. 
  • An autonomously self-assembling dendritic DNA nanostructure for target DNA detection. 
  • Analysis of allosteric signal transduction mechanisms in an engineered fluorescent maltose biosensor. 
  • Analysis of total uncertainty in spectral peak measurements for plasmonic nanoparticle-based biosensors. 
  • Antibody-antigen binding kinetics. A model for multivalency antibodies for large antigen systems. 
  • Antibody-based biosensor for breast cancer with ultrasonic regeneration. 
  • Application of a miniature biochip using the molecular beacon probe in breast cancer gene BRCA1 detection. 
  • Application of an antibody biochip for p53 detection and cancer diagnosis. 
  • Aromatic Functionality of Target Proteins Influences Monomer Selection for Creating Artificial Antibodies on Plasmonic Biosensors. 
  • Attachment of gold nanoparticles to glassy carbon electrode and its application for the direct electrochemistry and electrocatalytic behavior of hemoglobin. 
  • Bimodal behavior and isobestic transition pathway in surface plasmon resonance sensing. 
  • Bimodal liquid biopsy for cancer immunotherapy based on peptide engineering and nanoscale analysis. 
  • Binding and signaling of surface-immobilized reagentless fluorescent biosensors derived from periplasmic binding proteins. 
  • Biocompatibility studies of functionalized regioregular poly(3-hexylthiophene) layers for sensing applications. 
  • Bioluminescence imaging of glucose in tissue surrounding polyurethane and glucose sensor implants. 
  • Biomaterials community examines biosensor biocompatibility. 
  • Bioplasmonic paper-based assay for perilipin-2 non-invasively detects renal cancer. 
  • Bioresorbable photonic devices for the spectroscopic characterization of physiological status and neural activity. 
  • Biosensor system for continuous monitoring of organophosphate aerosols. 
  • Biosensors and biochips: advances in biological and medical diagnostics. 
  • Capacitive detection of glucose using molecularly imprinted polymers. 
  • Characterization of implantable biosensor membrane biofouling. 
  • Characterization of porous, dexamethasone-releasing polyurethane coatings for glucose sensors. 
  • Chip-scale sensor system integration for portable health monitoring. 
  • Chromosomally located gene fusions constructed in Acinetobacter sp. ADP1 for the detection of salicylate. 
  • Comparison of colloidal gold electrode fabrication methods: the preparation of a horseradish peroxidase enzyme electrode. 
  • Computational design of receptor and sensor proteins with novel functions. 
  • Computational design of receptors for an organophosphate surrogate of the nerve agent soman. 
  • Construction of a fluorescent biosensor family. 
  • Construction, imaging, and analysis of FRET-based tension sensors in living cells. 
  • Continuous monitoring of interstitial tissue oxygen using subcutaneous oxygen microsensors: In vivo characterization in healthy volunteers. 
  • Conversion of a maltose receptor into a zinc biosensor by computational design. 
  • DNA biochip using a phototransistor integrated circuit. 
  • Decreased analyte transport through implanted membranes: differentiation of biofouling from tissue effects. 
  • Design of bioelectronic interfaces by exploiting hinge-bending motions in proteins. 
  • Detection and identification of bacteria using antibiotic susceptibility and a multi-array electrochemical sensor with pattern recognition. 
  • Detection of E. coli using a microfluidics-based antibody biochip detection system. 
  • Detection of cytochrome C in a single cell using an optical nanobiosensor. 
  • Detection of flap tissue ischemia in a rat model: Real-time monitoring of changes in oxygenation and perfusion through injectable biosensors. 
  • Development of a new capillary electrophoresis-based fibre optic sensor. 
  • Development of antibody-based fiber-optic sensors for detection of a benzo[a]pyrene metabolite. 
  • Digital Health: Tracking Physiomes and Activity Using Wearable Biosensors Reveals Useful Health-Related Information 
  • Digital microfluidic chips for chemical and biological applications. 
  • Direct Optical Imaging of Graphene In Vitro by Nonlinear Femtosecond Laser Spectral Reshaping 
  • Direct SERDS sensing of molecular biomarkers in plants under field conditions. 
  • Direct electrochemistry of hemoglobin in egg-phosphatidylcholine films and its catalysis to H(2)O(2). 
  • Discovery of Thermostable, Fluorescently Responsive Glucose Biosensors by Structure-Assisted Function Extrapolation. 
  • Distinct conformational changes in beta-arrestin report biased agonism at seven-transmembrane receptors. 
  • Electrochemical biosensor of nanocube-augmented carbon nanotube networks. 
  • Emerging applications of stimuli-responsive polymer materials. 
  • Encoded multichromophore response for simultaneous label-free detection. 
  • Engineered microbial consortia: strategies and applications. 
  • Engineered nanomaterials for biophotonics applications: improving sensing, imaging, and therapeutics. 
  • Enhanced Release of Molecules upon Ultraviolet (UV) Light Irradiation from Photoresponsive Hydrogels Prepared from Bifunctional Azobenzene and Four-Arm Poly(ethylene glycol). 
  • Enhancing four-wave-mixing processes by nanowire arrays coupled to a gold film. 
  • Evaluation of the YSI 2300 glucose analyzer: algorithm-corrected results are accurate and specific. 
  • Experiences of closed-loop insulin delivery among pregnant women with Type 1 diabetes. 
  • Fabrication of nitric oxide-releasing polyurethane glucose sensor membranes. 
  • Femtomolar sensitivity using a channel-etched thin film waveguide fluoroimmunosensor. 
  • Ferroelectric thin films in fluidic environments: a new interface for sensing and manipulation of matter. 
  • Fiber-optic nanosensors for single-cell monitoring. 
  • Fully printed prothrombin time sensor for point-of-care testing. 
  • Functional nanostructures from surface chemistry patterning. 
  • G protein-coupled receptor (GPCR) signaling via heterotrimeric G proteins from endosomes. 
  • G protein-coupled receptor desensitization as a measure of signaling: modeling of arrestin recruitment to activated CCK-B receptors. 
  • G protein-coupled receptor kinase and beta-arrestin-mediated desensitization of the angiotensin II type 1A receptor elucidated by diacylglycerol dynamics. 
  • Genetic engineering of a single-chain antibody fragment for surface immobilization in an optical biosensor. 
  • Glucose sensing by MondoA:Mlx complexes: a role for hexokinases and direct regulation of thioredoxin-interacting protein expression. 
  • High-affinity, protective antibodies to the binding domain of botulinum neurotoxin type A. 
  • Hydrogen peroxide biosensor based on microperoxidase-11 entrapped in lipid membrane. 
  • Identification and characterization of a new cross-reactive human immunodeficiency virus type 1-neutralizing human monoclonal antibody. 
  • Identification of cognate ligands for the Escherichia coli phnD protein product and engineering of a reagentless fluorescent biosensor for phosphonates. 
  • Identification of troponin C antagonists from a phage-displayed random peptide library. 
  • Immobilized molecular beacons: a new strategy using UV-activated poly(methyl methacrylate) surfaces to provide large fluorescence sensitivities for reporting on molecular association events. 
  • Implant healing in experimental animal models of diabetes. 
  • Implanted Nanosensors in Marine Organisms for Physiological Biologging: Design, Feasibility, and Species Variability. 
  • Improving Quality, Reproducibility, and Usability of FRET-Based Tension Sensors. 
  • In vitro and in vivo characterization of porous poly-L-lactic acid coatings for subcutaneously implanted glucose sensors. 
  • In vitro, in vivo and post explantation testing of glucose-detecting biosensors: current methods and recommendations. 
  • Injectable Phosphorescence-based Oxygen Biosensors Identify Post Ischemic Reactive Hyperoxia. 
  • Intensified biochip system using chemiluminescence for the detection of Bacillus globigii spores. 
  • Intracellular measurements in mammary carcinoma cells using fiber-optic nanosensors. 
  • Intravital imaging of Ca(2+) signals in lymphocytes of Ca(2+) biosensor transgenic mice: indication of autoimmune diseases before the pathological onset. 
  • Intravital microscopy evaluation of angiogenesis and its effects on glucose sensor performance. 
  • Ion-channel sensing of ferricyanide anion based on a supported bilayer lipid membrane. 
  • Label free colorimetric biosensing using nanoparticles. 
  • Label-free DNA biosensor based on SERS Molecular Sentinel on Nanowave chip. 
  • Label-free plasmonic detection of biomolecular binding by a single gold nanorod. 
  • Lipid membrane immobilized horseradish peroxidase biosensor for amperometric determination of hydrogen peroxide. 
  • Live-cell Measurement of Odorant Receptor Activation Using a Real-time cAMP Assay. 
  • Liver injury-on-a-chip: microfluidic co-cultures with integrated biosensors for monitoring liver cell signaling during injury. 
  • Macrophage embedded fibrin gels: an in vitro platform for assessing inflammation effects on implantable glucose sensors. 
  • Measuring mechanical tension across vinculin reveals regulation of focal adhesion dynamics 
  • Mechano-acoustic sensing of physiological processes and body motions via a soft wireless device placed at the suprasternal notch. 
  • Metal nanoshells. 
  • Metallic Nanoislands on Graphene as Highly Sensitive Transducers of Mechanical, Biological, and Optical Signals. 
  • Metalloprotein design. 
  • Microcantilever sensing and actuation with end-grafted stimulus-responsive elastin-like polypeptides. 
  • Modeling of a foamed emulsion bioreactor: II. model parametric sensitivity. 
  • Modeling the Physiological Factors Affecting Glucose Sensor Function in Vivo. 
  • Modeling the relative impact of capsular tissue effects on implanted glucose sensor time lag and signal attenuation. 
  • Molecular sentinel-on-chip for SERS-based biosensing. 
  • Multi-analyte analysis system using an antibody-based biochip. 
  • Multicomponent patterning of indium tin oxide. 
  • Multiplex detection of disease biomarkers using SERS molecular sentinel-on-chip. 
  • Multiplexed GTPase and GEF biosensor imaging enables network connectivity analysis. 
  • N-terminal PDZ domain is required for NHERF dimerization. 
  • Nanobiosensors: probing the sanctuary of individual living cells. 
  • Nanoprobes and nanobiosensors for monitoring and imaging individual living cells. 
  • Nanosensor for in vivo measurement of the carcinogen benzo[a]pyrene in a single cell. 
  • Narrow groove plasmonic nano-gratings for surface plasmon resonance sensing. 
  • Neural circuits. Labeling of active neural circuits in vivo with designed calcium integrators. 
  • New technology and clinical applications of nanomedicine: highlights of the second annual meeting of the American Academy of Nanomedicine (Part I). 
  • Optical nanobiosensor for monitoring an apoptotic signaling process in a single living cell following photodynamic therapy. 
  • Optical nanosensors for detecting proteins and biomarkers in individual living cells. 
  • Optical sensor for the detection of caspase-9 activity in a single cell. 
  • PEGylated Artificial Antibodies: Plasmonic Biosensors with Improved Selectivity. 
  • Peptide Functionalized Gold Nanorods for the Sensitive Detection of a Cardiac Biomarker Using Plasmonic Paper Devices. 
  • Peptide-Functionalized Fluorescent Particles for In Situ Detection of Nitric Oxide via Peroxynitrite-Mediated Nitration. 
  • Personalized diagnostics and biosensors: a review of the biology and technology needed for personalized medicine. 
  • Pharmacological characterization of membrane-expressed human trace amine-associated receptor 1 (TAAR1) by a bioluminescence resonance energy transfer cAMP biosensor. 
  • Phosphorylation of Src by phosphoinositide 3-kinase regulates beta-adrenergic receptor-mediated EGFR transactivation. 
  • Plasmonic Nanoprobes for in Vivo Multimodal Sensing and Bioimaging of MicroRNA within Plants. 
  • Plasmonic SERS biosensing nanochips for DNA detection. 
  • Plasmonic detection of a model analyte in serum by a gold nanorod sensor. 
  • Plasmonic gold nanostars for multi-modality sensing and diagnostics. 
  • Plasmonic nanobiosensors for detection of microRNA cancer biomarkers in clinical samples. 
  • Plasmonic nanoprobes: from chemical sensing to medical diagnostics and therapy. 
  • Plasmonics nanoprobes: detection of single-nucleotide polymorphisms in the breast cancer BRCA1 gene. 
  • Pre-validation of choriogenin H transgenic medaka eleutheroembryos as a quantitative estrogenic activity test method. 
  • Programmable assembly of pressure sensors using pattern-forming bacteria. 
  • Protein engineering and the development of generic biosensors. 
  • Protonophore- and pH-insensitive glucose and sucrose accumulation detected by FRET nanosensors in Arabidopsis root tips. 
  • Psychedelic-inspired drug discovery using an engineered biosensor. 
  • Qualitative and quantitative differences in T cell receptor binding of agonist and antagonist ligands. 
  • Quantitative in situ assay of salicylic acid in tobacco leaves using a genetically modified biosensor strain of Acinetobacter sp. ADP1. 
  • Quantum dots-bienzyme hybrid system for the sensitive determination of glucose. 
  • Rational selection of gold nanorod geometry for label-free plasmonic biosensors. 
  • Real-Time 3D Single Particle Tracking: Towards Active Feedback Single Molecule Spectroscopy in Live Cells. 
  • Review of methods used to study oxygen transport at the microcirculatory level. 
  • SERS nanosensors and nanoreporters: golden opportunities in biomedical applications. 
  • SERS opens a new way in aptasensor for protein recognition with high sensitivity and selectivity. 
  • SERS-based plasmonic nanobiosensing in single living cells. 
  • Screening for the breast cancer gene (BRCA1) using a biochip system and molecular beacon probes immobilized on solid surfaces. 
  • Seahorse Xfe 24 Extracellular Flux Analyzer-Based Analysis of Cellular Respiration in Caenorhabditis elegans. 
  • Sensing of molecules using quantum dynamics. 
  • Sensitive DNA detection and SNP discrimination using ultrabright SERS nanorattles and magnetic beads for malaria diagnostics. 
  • Shear stress induces ATP-independent transient nitric oxide release from vascular endothelial cells, measured directly with a porphyrinic microsensor. 
  • Silk-Encapsulated Plasmonic Biochips with Enhanced Thermal Stability. 
  • Simple and sensitive aptamer-based colorimetric sensing of protein using unmodified gold nanoparticle probes. 
  • Single Molecule Force Spectroscopy to Compare Natural versus Artificial Antibody-Antigen Interaction. 
  • Single- and dual-fractal analysis of hybridization binding kinetics: biosensor applications. 
  • Single-cell monitoring using fiberoptic nanosensors. 
  • Software for lattice light-sheet imaging of FRET biosensors, illustrated with a new Rap1 biosensor. 
  • Spatiotemporal dynamics of GEF-H1 activation controlled by microtubule- and Src-mediated pathways. 
  • Structure-based design of robust glucose biosensors using a Thermotoga maritima periplasmic glucose-binding protein. 
  • Surface engineering for efficient capture of circulating tumor cells in renal cell carcinoma: From nanoscale analysis to clinical application. 
  • Synthesis and characterization of a ruthenium(II)-based redox conjugate for reagentless biosensing. 
  • Synthesis and electrochemical applications of gold nanoparticles. 
  • The detection and role of molecular tension in focal adhesion dynamics. 
  • The development of optical nanosensors for biological measurements. 
  • The electrical conductivity of phospholipid films as an iodine-sensing mechanism. 
  • The rational design of allosteric interactions in a monomeric protein and its applications to the construction of biosensors. 
  • Tissue-Integrating Oxygen Sensors: Continuous Tracking of Tissue Hypoxia. 
  • Tunable molecular tension sensors reveal extension-based control of vinculin loading. 
  • Ultrarobust Biochips with Metal-Organic Framework Coating for Point-of-Care Diagnosis. 
  • Using genetically encoded fluorescent biosensors to interrogate ovarian cancer metabolism. 
  • Utilizing a CdTe quantum dots-enzyme hybrid system for the determination of both phenolic compounds and hydrogen peroxide. 
  • Wearable sensors enable personalized predictions of clinical laboratory measurements. 
  • beta2-adrenergic receptor signaling and desensitization elucidated by quantitative modeling of real time cAMP dynamics. 
  • miRNA probe integrated biosensor platform using bimetallic nanostars for amplification-free multiplexed detection of circulating colorectal cancer biomarkers in clinical samples. 
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  Keywords of People

  • Beratan, David N., R.J. Reynolds Distinguished Professor of Chemistry, Nicholas Institute-Energy Initiative
  • Driehuys, Bastiaan, Professor of Radiology, Biomedical Engineering
  • Ginsburg, Geoffrey Steven, Adjunct Professor in the Department of Medicine, School of Nursing
  • Hoffman, Brenton D., James L. and Elizabeth M. Vincent Associate Professor of Biomedical Engineering, Biomedical Engineering
  • Jokerst, Nan Marie, J. A. Jones Distinguished Professor of Electrical and Computer Engineering, Nicholas Institute-Energy Initiative
  • Muir, Kelly Walton, Associate Professor of Ophthalmology, Ophthalmology, Glaucoma
  • Nicolelis, Miguel Angelo L., Duke School of Medicine Distinguished Professor in Neuroscience, Neurobiology
  • Wax, Adam P., Professor of Biomedical Engineering, Biomedical Engineering