Gene Library

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  Subject Areas on Research

  • A CaMK cascade activates CRE-mediated transcription in neurons of Caenorhabditis elegans. 
  • A SAGE (serial analysis of gene expression) view of breast tumor progression. 
  • A SAGE study of apolipoprotein E3/3, E3/4 and E4/4 allele-specific gene expression in hippocampus in Alzheimer disease. 
  • A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila. 
  • A chromosome jump crosses a translocation breakpoint in the von Recklinghausen neurofibromatosis region. 
  • A comparative analysis of the information content in long and short SAGE libraries. 
  • A comparison of sequencing platforms and bioinformatics pipelines for compositional analysis of the gut microbiome. 
  • A framework for integrating the songbird brain. 
  • A gene hunting we will go! 
  • A genome-wide visual screen reveals a role for sphingolipids and ergosterol in cell surface delivery in yeast. 
  • A highly parallel method for synthesizing DNA repeats enables the discovery of 'smart' protein polymers. 
  • A lentiviral RNAi library for human and mouse genes applied to an arrayed viral high-content screen. 
  • A library approach for the discovery of customized yeast three-hybrid counter selections. 
  • A method for linking VL and VH region genes that allows bulk transfer between vectors for use in generating polyclonal IgG libraries. 
  • A novel Ca2+/calmodulin-dependent protein kinase and a male germ cell-specific calmodulin-binding protein are derived from the same gene. 
  • A novel alternative splice domain in zebrafish tenascin-C. 
  • A novel mechanism for regulating transforming growth factor beta (TGF-beta) signaling. Functional modulation of type III TGF-beta receptor expression through interaction with the PDZ domain protein, GIPC. 
  • A role for Cdc2- and PP2A-mediated regulation of Emi2 in the maintenance of CSF arrest. 
  • A small interfering RNA screen for modulators of tumor cell motility identifies MAP4K4 as a promigratory kinase. 
  • A system of transposon mutagenesis for bacteriophage T4. 
  • A tarantula-venom peptide antagonizes the TRPA1 nociceptor ion channel by binding to the S1-S4 gating domain. 
  • A uniform human Wnt expression library reveals a shared secretory pathway and unique signaling activities. 
  • A yeast artificial chromosome contig encompassing the type 1 neurofibromatosis gene. 
  • Aberrant allele frequencies of the SNPs located in microRNA target sites are potentially associated with human cancers. 
  • Acquisition of a side population fraction augments malignant phenotype in ovarian cancer. 
  • An integrated physical map of 8q22-q24: use in positional cloning and deletion analysis of Langer-Giedion syndrome. 
  • Analysis of recombinant Phex: an endopeptidase in search of a substrate. 
  • Analysis of the mouse transcriptome for genes involved in the function of the nervous system. 
  • Angiotensin converting enzyme and genetic hypertension: cloning of rat cDNAs and characterization of the enzyme. 
  • Aptamers: an emerging class of therapeutics. 
  • Bacterial artificial chromosome cloning and mapping of a 630-kb human extrachromosomal structure. 
  • COT drives resistance to RAF inhibition through MAP kinase pathway reactivation. 
  • CRISPR Library Screening in Cultured Cardiomyocytes. 
  • CTD kinase large subunit is encoded by CTK1, a gene required for normal growth of Saccharomyces cerevisiae. 
  • Cell-internalization SELEX: method for identifying cell-internalizing RNA aptamers for delivering siRNAs to target cells. 
  • Characterization and expression of a rice RAD23 gene. 
  • Characterization and expression of the complementary DNA encoding rat histidine decarboxylase. 
  • Characterization of de novo duplications in eight patients by using fluorescence in situ hybridization with chromosome-specific DNA libraries. 
  • Characterization of four novel ras-like genes expressed in a human teratocarcinoma cell line. 
  • Characterization of kinectin, a kinesin-binding protein: primary sequence and N-terminal topogenic signal analysis. 
  • Characterization of the DNase I hypersensitive site 3' of the human beta globin gene domain. 
  • Characterization of the murine BEK fibroblast growth factor (FGF) receptor: activation by three members of the FGF family and requirement for heparin. 
  • Chromosomal localization of human glutamate receptor genes. 
  • Circadian clock-controlled genes isolated from Neurospora crassa are late night- to early morning-specific. 
  • Circular transcripts of the testis-determining gene Sry in adult mouse testis. 
  • Clarification of subtle reciprocal rearrangements using fluorescence in situ hybridization. 
  • Cloning and expression of a novel, truncated, progesterone receptor. 
  • Cloning of cDNA and analysis of the gene for mouse angiotensin II type 2 receptor. 
  • Complete sequence of the Drosophila nonmuscle myosin heavy-chain transcript: conserved sequences in the myosin tail and differential splicing in the 5' untranslated sequence. 
  • Construction of cDNA libraries from small amounts of total RNA using the suppression PCR effect. 
  • Convergent evolution of chromosomal sex-determining regions in the animal and fungal kingdoms. 
  • Cryptococcus neoformans gene expression during experimental cryptococcal meningitis. 
  • D21S194, a jump clone from D21S16. 
  • DNase-seq: a high-resolution technique for mapping active gene regulatory elements across the genome from mammalian cells. 
  • DO-RIP-seq to quantify RNA binding sites transcriptome-wide. 
  • Deep sequencing of the small RNA transcriptome of normal and malignant human B cells identifies hundreds of novel microRNAs. 
  • Deep-RACE: Comprehensive Search for Novel ncRNAs Associated to a Specific Locus. 
  • Defining the human macula transcriptome and candidate retinal disease genes using EyeSAGE. 
  • Design and implementation of high-throughput RNAi screens in cultured Drosophila cells. 
  • Detailed analysis of gene expression during development of T cell lineages in the thymus. 
  • Differential display RT-PCR for identifying novel gene expression in the lung. 
  • Differential expression of gamma-aminobutyric acidA receptor subunits. 
  • Differential screening of a human chromosome 3 library identifies hepatocyte growth factor-like/macrophage-stimulating protein and its receptor in injured lung. Possible implications for neuroendocrine cell survival. 
  • Differential screening of a subtracted cDNA library: a method to search for genes preferentially expressed in multiple tissues. 
  • Directed evolution of a new catalytic site in 2-keto-3-deoxy-6-phosphogluconate aldolase from Escherichia coli. 
  • Directed evolution of subtilisin E in Bacillus subtilis to enhance total activity in aqueous dimethylformamide. 
  • Discovery of the Elusive UDP-Diacylglucosamine Hydrolase in the Lipid A Biosynthetic Pathway in Chlamydia trachomatis. 
  • Distinct polyadenylation landscapes of diverse human tissues revealed by a modified PA-seq strategy. 
  • Distribution and diversity of planktonic fungi in the West Pacific Warm Pool. 
  • Dynamic microRNA gene transcription and processing during T cell development. 
  • EP24.15 interacts with the angiotensin II type I receptor and bradykinin B2 receptor. 
  • Efficacy and safety of next-generation tick transcriptome-derived direct thrombin inhibitors. 
  • Engineered leucine zippers show that hemiphosphorylated CREB complexes are transcriptionally active. 
  • Engineering and Selection of Shuffled AAV Genomes: A New Strategy for Producing Targeted Biological Nanoparticles. 
  • Evaluation of 23S rRNA PCR primers for use in phylogenetic studies of bacterial diversity. 
  • Evidence of off-target effects associated with long dsRNAs in Drosophila melanogaster cell-based assays. 
  • Exploring molecular diversity with combinatorial shape libraries. 
  • Expressed sequence tag analysis in Cycas, the most primitive living seed plant. 
  • Expression of a variant form of the glutamate transporter GLT1 in neuronal cultures and in neurons and astrocytes in the rat brain. 
  • Fine-scale phylogenetic architecture of a complex bacterial community. 
  • FlyRNAi: the Drosophila RNAi screening center database. 
  • GIPC interacts with the beta1-adrenergic receptor and regulates beta1-adrenergic receptor-mediated ERK activation. 
  • Generating RNA Baits for Capture-Based Enrichment. 
  • Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. 
  • Generation of full-length cDNA libraries enriched for differentially expressed genes for functional genomics. 
  • Generation of full-length cDNA libraries enriched for differentially expressed genes. 
  • Generation of random mutant libraries with multiple primers in a single reaction. 
  • Generation of species cross-reactive aptamers using "toggle" SELEX. 
  • Genetically encoded synthesis of protein-based polymers with precisely specified molecular weight and sequence by recursive directional ligation: examples from the elastin-like polypeptide system. 
  • Genome maps 7. The human transcript map. Wall chart. 
  • Genome-wide analysis of central corneal thickness in primary open-angle glaucoma cases in the NEIGHBOR and GLAUGEN consortia. 
  • Genome-wide localization of replication factors. 
  • Genomic cloning of hGSTP1*C, an allelic human Pi class glutathione S-transferase gene variant and functional characterization of its retinoic acid response elements. 
  • Genomic convergence to identify candidate genes for Parkinson disease: SAGE analysis of the substantia nigra. 
  • Genomic convergence: identifying candidate genes for Parkinson's disease by combining serial analysis of gene expression and genetic linkage. 
  • Genomic organization, expression, and localization of murine Ran-binding protein 2 (RanBP2) gene. 
  • Glucokinase and glucose transporter expression in liver and islets: implications for control of glucose homoeostasis. 
  • Growth arrest and DNA damage-inducible protein GADD34 assembles a novel signaling complex containing protein phosphatase 1 and inhibitor 1. 
  • HIN-1, a putative cytokine highly expressed in normal but not cancerous mammary epithelial cells. 
  • HTS-Compatible CometChip Enables Genetic Screening for Modulators of Apoptosis and DNA Double-Strand Break Repair. 
  • Homogeneous reporter system enables quantitative functional assessment of multiple transcription factors. 
  • Human apolipoprotein E2, E3, and E4 isoform-specific transgenic mice: human-like pattern of glial and neuronal immunoreactivity in central nervous system not observed in wild-type mice. 
  • Human retroviral gag- and gag-pol-like proteins interact with the transforming growth factor-beta receptor activin receptor-like kinase 1. 
  • Hyperosmotic stress stimulates promoter activity and regulates cellular utilization of the serum- and glucocorticoid-inducible protein kinase (Sgk) by a p38 MAPK-dependent pathway. 
  • Identification and characterization of a cDNA encoding cytochrome P450 3A from the fresh water teleost medaka (Oryzias latipes). 
  • Identification of Drosophila cytoskeletal proteins by induction of abnormal cell shape in fission yeast. 
  • Identification of a new family of protein phosphatase 2A regulatory subunits. 
  • Identification of casein kinase I substrates by in vitro expression cloning screening. 
  • Identification of genes expressed in a human scleral cDNA library. 
  • Identification of genes expressed in the Xenopus inner ear. 
  • Identification of variants in CNGA3 as cause for achromatopsia by exome sequencing of a single patient. 
  • In vitro and in vivo behavior of radiolabeled chimeric anti-EGFRvIII monoclonal antibody: comparison with its murine parent. 
  • In vitro selection of aptamers from RNA libraries. 
  • Induction of a human carbonyl reductase gene located on chromosome 21. 
  • Integrative genomic approaches identify IKBKE as a breast cancer oncogene. 
  • Interplay between SCARECROW, GA and LIKE HETEROCHROMATIN PROTEIN 1 in ground tissue patterning in the Arabidopsis root. 
  • Isolation and functional analysis of the mouse RXRgamma1 gene promoter in anterior pituitary cells. 
  • Isolation and initial characterization of the 5' flanking region of the human and murine cyclic guanosine monophosphate-phosphodiesterase beta-subunit genes. 
  • Isolation of a candidate cDNA for the gene causing retinal degeneration in the rd mouse. 
  • Mating-type locus of Cryptococcus neoformans: a step in the evolution of sex chromosomes. 
  • Mechanistic studies of human molybdopterin synthase reaction and characterization of mutants identified in group B patients of molybdenum cofactor deficiency. 
  • MegaGate: A toxin-less gateway molecular cloning tool. 
  • Microarray gene expression profiles in dilated and hypertrophic cardiomyopathic end-stage heart failure. 
  • Mitochondrial FtsZ in a chromophyte alga 
  • Mitochondrial biogenesis in striated muscles: rapid induction of citrate synthase mRNA by nerve stimulation. 
  • Modulation of glutathione peroxidase expression by selenium: effect on human MCF-7 breast cancer cell transfectants expressing a cellular glutathione peroxidase cDNA and doxorubicin-resistant MCF-7 cells. 
  • Molecular characterization and localization of the human MAFG gene. 
  • Molecular characterization of an arachnid sodium channel gene from the varroa mite (Varroa destructor). 
  • Molecular cloning and expression of cDNA encoding a peripheral-type benzodiazepine receptor. 
  • Molecular cloning of protein phosphatase inhibitor-1 and its expression in rat and rabbit tissues. 
  • Molecular markers in ductal carcinoma in situ of the breast. 
  • Molecular structure of a protein-tyrosine/threonine kinase activating p42 mitogen-activated protein (MAP) kinase: MAP kinase kinase. 
  • Multiassay Profiling of a Focused Small Molecule Library Reveals Predictive Bidirectional Modulation of the lncRNA MALAT1 Triplex Stability In Vitro . 
  • Multiplex-PCR-based recombination as a novel high-fidelity method for directed evolution. 
  • Muscle-specific RING finger 1 is a bona fide ubiquitin ligase that degrades cardiac troponin I. 
  • Myocyte nuclear factor, a novel winged-helix transcription factor under both developmental and neural regulation in striated myocytes. 
  • NEIBank: genomics and bioinformatics resources for vision research. 
  • Next-generation sequencing of the human olfactory receptors. 
  • Novel bacterial P-type ATPases with histidine-rich heavy-metal-associated sequences. 
  • Novel enriched pathways in superficial malignant peripheral nerve sheath tumours and spindle/desmoplastic melanomas. 
  • Osteopontin inhibits expression of cytochrome c oxidase in RAW 264.7 murine macrophages. 
  • PR48, a novel regulatory subunit of protein phosphatase 2A, interacts with Cdc6 and modulates DNA replication in human cells. 
  • Phospholipase C-beta 2 interacts with mitogen-activated protein kinase kinase 3. 
  • Photoautotrophic symbiont and geography are major factors affecting highly structured and diverse bacterial communities in the lichen microbiome. 
  • Physical and cDNA mapping in the DBH region of human chromosome 9q34. 
  • Polymorphism and altered methylation of the lactoferrin gene in normal leukocytes, leukemic cells, and breast cancer. 
  • Primary structure and domain organization of human alpha and beta adducin. 
  • Programmable mutually exclusive alternative splicing for generating RNA and protein diversity. 
  • Quantitative determination that one of two potential RNA-binding domains of the A protein component of the U1 small nuclear ribonucleoprotein complex binds with high affinity to stem-loop II of U1 RNA. 
  • RNA interference and single particle tracking analysis of hepatitis C virus endocytosis. 
  • Rapid identification of cell-specific, internalizing RNA aptamers with bioinformatics analyses of a cell-based aptamer selection. 
  • Recombinant immunotoxins specific for a mutant epidermal growth factor receptor: targeting with a single chain antibody variable domain isolated by phage display. 
  • Recombinant polyclonal antibody libraries. 
  • Regulation of ALK-1 signaling by the nuclear receptor LXRbeta. 
  • Regulation of both PDK1 and the phosphorylation of PKC-zeta and -delta by a C-terminal PRK2 fragment. 
  • Regulatory and structural motifs of chicken gizzard myosin light chain kinase. 
  • Reiterative Recombination for the in vivo assembly of libraries of multigene pathways. 
  • Report and abstracts of the 4th International Workshop on Chromosome 9. Williamsburg, Virginia, USA, April 23-25, 1995. 
  • Responses of soil cellulolytic fungal communities to elevated atmospheric CO₂ are complex and variable across five ecosystems. 
  • Retina-specifically expressed novel subtypes of bovine cyclophilin. 
  • Reverse genetics of Drosophila RNA polymerase II: identification and characterization of RpII140, the genomic locus for the second-largest subunit. 
  • Screen and identification of proteins interacting with ADAM19 cytoplasmic tail. 
  • Screening Regulatory Element Function with CRISPR/Cas9-based Epigenome Editing. 
  • Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1. 
  • Serial analysis of gene expression (SAGE) in normal human trabecular meshwork. 
  • Sex dependent glial-specific changes in the chromatin accessibility landscape in late-onset Alzheimer's disease brains. 
  • Small-Molecule Positive Allosteric Modulators of the β2-Adrenoceptor Isolated from DNA-Encoded Libraries. 
  • Statistical analysis of genetic interactions in Tn-Seq data. 
  • Structural characterization of the 5' regions of the human phenylalanine hydroxylase gene. 
  • Structure and function of the ovine type 1 corticotropin releasing factor receptor (CRF1) and a carboxyl-terminal variant. 
  • Structure and regulation of the mouse ing1 gene. Three alternative transcripts encode two phd finger proteins that have opposite effects on p53 function. 
  • Subtractive cloning: new genes for studying inflammatory disorders. 
  • Suppression of a P450 hydroxylase gene in plant trichome glands enhances natural-product-based aphid resistance. 
  • Suppression subtractive hybridization: a versatile method for identifying differentially expressed genes. 
  • The genetic structure and diversity of the A and B mating-type genes from the tropical oyster mushroom, Pleurotus djamor. 
  • The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans. 
  • The high Km glucose transporter of islets of Langerhans is functionally similar to the low affinity transporter of liver and has an identical primary sequence. 
  • The role of carbohydrate in the function of human plasminogen: comparison of the protein obtained from molecular cloning and expression in Escherichia coli and COS cells. 
  • Transcriptome analysis of mouse stem cells and early embryos. 
  • Urinary Single-Cell Profiling Captures the Cellular Diversity of the Kidney. 
  • Use of phage display to identify novel mineralocorticoid receptor-interacting proteins. 
  • Zebrafish Transcription Factor ORFeome for Gene Discovery and Regulatory Network Elucidation. 
  • bcSeq: an R package for fast sequence mapping in high-throughput shRNA and CRISPR screens. 
  • beta 1-adrenergic receptor association with PSD-95. Inhibition of receptor internalization and facilitation of beta 1-adrenergic receptor interaction with N-methyl-D-aspartate receptors. 
  • cDNA cloning and expression of rat and human protein geranylgeranyltransferase type-I. 
  • iPABP, an inducible poly(A)-binding protein detected in activated human T cells. 
  • p38 MAPK is essential for secondary axis specification and patterning in sea urchin embryos. 
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  Keywords of People

  • Bennett, Vann, George Barth Geller Distinguished Professor of Molecular Biology, Duke Cancer Institute
  • Bowes Rickman, Catherine, George and Geneva Boguslavsky Distinguished Professor of Eye Research, Cell Biology
  • Ciofani, Maria, Associate Professor of Integrative Immunobiology, Cell Biology
  • Erickson, Harold Paul, James B. Duke Distinguished Professor Emeritus, Cell Biology
  • Ferreira, Paulo Alexandre, Associate Professor in Ophthalmology, Pathology
  • Hartemink, Alexander J., Professor in the Department of Computer Science, Biology
  • Hogan, Brigid L. M., Research Professor of Cell Biology, Cell Biology
  • Liu, Yutao, Adjunct Assistant Professor in the Department of Medicine, Medicine, Medical Genetics
  • Luftig, Micah Alan, Professor of Molecular Genetics and Microbiology, Cell Biology
  • McDonnell, Donald Patrick, Glaxo-Wellcome Distinguished Professor of Molecular Cancer Biology, in the School of Medicine, Cell Biology
  • Shinohara, Mari L., Professor of Integrative Immunobiology, Cell Biology