Cryopreservation

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  Subject Areas on Research

  • A protocol for imaging alternative splicing regulation in vivo using fluorescence reporters in transgenic mice. 
  • A step toward solving some of the problems of cryopreservation. 
  • Actin cytoskeletal disruption following cryopreservation alters the biodistribution of human mesenchymal stromal cells in vivo. 
  • Adolescent cancer patients: sperm storage, consent and emotion. 
  • Adult dual umbilical cord blood transplantation using myeloablative total body irradiation (1350 cGy) and fludarabine conditioning. 
  • Angiogenesis and growth of isografted bone: quantitative in vivo assay in nude mice. 
  • Automated thawing increases recovery of colony-forming units from banked cord blood unit grafts. 
  • Basal follicle-stimulating hormone level and age affect the chance for and outcome of pre-embryo cryopreservation. 
  • Calpain inhibition prevents sinusoidal endothelial cell apoptosis in the cold ischemic rat liver. 
  • Characterization of cord blood natural killer and lymphokine activated killer lymphocytes following ex vivo cellular engineering. 
  • Cold machine perfusion or static cold storage of kidneys: why the debate continues. 
  • Combined impact of the number of pre-ovulatory oocytes and cryopreservation on IVF outcome. 
  • Comparison of intracervical, intrauterine, and intratubal techniques for donor insemination. 
  • Conservation and regeneration of transgenic lines of swine by semen cryopreservation and artificial insemination. 
  • Controlled preparation of the endometrium with exogenous steroids for the transfer of frozen-thawed pre-embryos in patients with anovulatory or irregular cycles. 
  • Cord-blood transplants. 
  • Cryo-electron microscopy of biological samples. 
  • Cryopreservation of all prezygotes in patients at risk of severe hyperstimulation does not eliminate the syndrome, but the chances of pregnancy are excellent with subsequent frozen-thaw transfers. 
  • Cryopreservation: the problem of evaluation. 
  • Cryopreserved Mesenchymal Stromal Cells Are Susceptible to T-Cell Mediated Apoptosis Which Is Partly Rescued by IFNγ Licensing. 
  • Cryopreserved human amniotic membrane injection for plantar fasciitis: a randomized, controlled, double-blind pilot study. 
  • Cryopreserving whole blood for functional assays using viable lymphocytes in molecular epidemiology studies. 
  • Descemet-stripping automated endothelial keratoplasty: effect of anterior lamellar corneal tissue-on/-off storage condition on Descemet-stripping automated endothelial keratoplasty donor tissue. 
  • Development of a contemporary globally diverse HIV viral panel by the EQAPOL program. 
  • Donor hepatic function: a factor in postreperfusion syndrome. 
  • Donor specific bone marrow cells suppress lymphocyte reactivity to donor antigens and differentially modulate TH1 and TH2 cytokine gene expression in the responder cell population. 
  • Effect of hepatic artery flow on bile secretory function after cold ischemia. 
  • Effects of acetaminophen and nonsteroidal anti-inflammatory drugs on progesterone production by porcine granulosa cells in vitro. 
  • Effects of postmortem freezing on tensile failure properties of rabbit extensor digitorum longus muscle tendon complex. 
  • Establishment and maintenance of a PBMC repository for functional cellular studies in support of clinical vaccine trials. 
  • Esterified and unesterified cholesterol in drusen and basal deposits of eyes with age-related maculopathy. 
  • Ethical aspects of banking placental blood for transplantation. 
  • Ethical issues in gamete preservation for children undergoing treatment for cancer. 
  • Evaluation of the clinical efficacy of embryo cryopreservation. 
  • Evaluation of the spindle apparatus of in-vitro matured human oocytes following cryopreservation. 
  • Extended storage of AS-1 and AS-3 leukoreduced red blood cells for 15 days after deglycerolization and resuspension in AS-3 using an automated closed system. 
  • Factors that affect infertility patients' decisions about disposition of frozen embryos. 
  • Feasibility of vitrification as a storage method for tissue-engineered blood vessels. 
  • Fertility patients' views about frozen embryo disposition: results of a multi-institutional U.S. survey. 
  • Fertilization and in vitro development of cryopreserved human prophase I oocytes. 
  • Freezing of all embryos in in vitro fertilization is beneficial in high responders, but not intermediate and low responders: an analysis of 82,935 cycles from the Society for Assisted Reproductive Technology registry. 
  • Freezing techniques and results. 
  • Globoid cell leukodystrophy (Krabbe disease): normal umbilical cord blood galactocerebrosidase activity and polymorphic mutations. 
  • Hematopoietic cell transplantation with cord blood for cure of HIV infections. 
  • Hydroxyethyl starch as a substitute for dextran 40 for thawing peripheral blood progenitor cell products. 
  • Immunomagnetic selection of purified monocyte and lymphocyte populations from peripheral blood mononuclear cells following cryopreservation. 
  • Impact of Specimen Heterogeneity on Biomarkers in Repository Samples from Patients with Acute Myeloid Leukemia: A SWOG Report. 
  • Impact of a cryopreservation program on the multiple pregnancy rate associated with assisted reproductive technologies. 
  • Impact of cryopreservation on tetramer, cytokine flow cytometry, and ELISPOT. 
  • Impact of different clinical variables on pregnancy outcome following embryo cryopreservation. 
  • Impact of intracytoplasmic sperm injection on embryo cryopreservation and clinical outcome. 
  • In situ cryofixation of kidney for electron probe X-ray microanalysis. 
  • In situ reconstruction with cryopreserved arterial allografts for management of mycotic aneurysms or aortic prosthetic graft infections: a multi-institutional experience. 
  • In vitro and in vivo activity of murine lymphokine-activated killer cells after cryopreservation. 
  • Incidence of allergic reactions with fresh frozen plasma or cryo-supernatant plasma in the treatment of thrombotic thrombocytopenic purpura. 
  • Influence of number of pre-embryos transferred, progesterone level and oestradiol/progesterone ratio at thaw on pregnancy results during replacement of cryopreserved pre-embryos in natural cycles. 
  • Intracytoplasmic sperm injection: achievement of high pregnancy rates in couples with severe male factor infertility is dependent primarily upon female and not male factors. 
  • Isolation and expansion of oligodendrocyte progenitor cells from cryopreserved human umbilical cord blood. 
  • Isolation of early hematopoietic cells, including megakaryocyte progenitors, in the ALDH-bright cell population of cryopreserved, banked UC blood. 
  • Leukopak PBMC sample processing for preparing quality control material to support proficiency testing programs. 
  • Low-dose glucocorticoids after in vitro fertilization and embryo transfer have no significant effect on pregnancy rate. 
  • Manufacture of Autologous CD34+ Selected Grafts in the NIAID-Sponsored HALT-MS and SCOT Multicenter Clinical Trials for Autoimmune Diseases. 
  • Maximizing the retention of antigen specific lymphocyte function after cryopreservation. 
  • Measurement of cytokine release at the single cell level using the ELISPOT assay. 
  • Meniscal allograft transplantation: a systematic review. 
  • Mitochondrial localization and characterization of 99Tc-SESTAMIBI in heart cells by electron probe X-ray microanalysis and 99Tc-NMR spectroscopy. 
  • Mobilization and collection of peripheral blood CD34+ cells from patients with Fanconi anemia. 
  • Modeling of live-birth rates and cost-effectiveness of oocyte cryopreservation for cancer patients prior to high- and low-risk gonadotoxic chemotherapy. 
  • Modified simple limbal epithelial transplantation using cryopreserved amniotic membrane for unilateral limbal stem cell deficiency. 
  • Multiparameter-fluorescence activated cell sorting analysis of retroviral vector gene transfer into primitive umbilical cord blood cells. 
  • Mycotic Saccular Abdominal Aortic Aneurysm in an Infant after Cardiac Catheterization: A Case Report. 
  • Oocyte Cryopreservation for Fertility Preservation in Healthy Women. 
  • Optimal timing for elective egg freezing. 
  • Optimization of storage and shipment of cryopreserved peripheral blood mononuclear cells from HIV-infected and uninfected individuals for ELISPOT assays. 
  • Outcome of intracytoplasmic sperm injection in azoospermic patients: stressing the liaison between the urologist and reproductive medicine specialist. 
  • Performance of cryopreserved pre-embryos obtained in in vitro fertilization cycles with or without a gonadotropin-releasing hormone agonist. 
  • Peripheral blood stem cell harvesting and CD34-positive cell selection. 
  • Planning and response to radiation exposures. 
  • Polyvinyl Pyrrolidone: A Novel Cryoprotectant in Islet Cell Cryopreservation. 
  • Predictive value of pregnancy during original in vitro fertilization cycle on implantation and pregnancy in subsequent cryothaw cycles. 
  • Pregnancy following transfer of ooplasm from cryopreserved-thawed donor oocytes into recipient oocytes. 
  • Prevalence of a Good Perinatal Outcome With Cryopreserved Compared With Fresh Donor Oocytes. 
  • Procurement, preservation, and transport of cadaver kidneys. 
  • Progesterone levels on the day of human chorionic gonadotropin do not predict pregnancy outcome from the transfer of fresh or cryopreserved embryos from the same cohort. 
  • Protective effects of ischemic preconditioning on the cold-preserved liver are tyrosine kinase dependent. 
  • Quality assurance program for peripheral blood mononuclear cell cryopreservation. 
  • Quantifying skeletal muscle properties in cadaveric test specimens: effects of mechanical loading, postmortem time, and freezer storage. 
  • Quantitative estimation of cryopreservation viability in rat fetal hippocampal cells. 
  • Quick-freezing of cultured cardiac cells in situ with special attention to the mitochondrial ultrastructure. 
  • Rapid and Efficient Generation of Transgene-Free iPSC from a Small Volume of Cryopreserved Blood. 
  • Rapid freezing of whole blood or buffy coat samples for polymerase chain reaction and cell culture analysis: application to detection of human immunodeficiency virus in blood donor and recipient repositories. The Transfusion Safety Study Group. 
  • Real-time quantitative elemental analysis and mapping: microchemical imaging in cell physiology. 
  • Red blood cell phenotype fidelity following glycerol cryopreservation optimized for research purposes. 
  • Results of the cord blood transplantation (COBLT) study unrelated donor banking program. 
  • Sertoli cell ablation and replacement of the spermatogonial niche in mouse. 
  • Significantly enhanced pregnancy rates per cycle through cryopreservation and thaw of pronuclear stage oocytes. 
  • Standardization of cytokine flow cytometry assays. 
  • Structural and elemental characterization of heart cells grown in a collagen matrix. 
  • Subtalar distraction arthrodesis using interpositional frozen structural allograft. 
  • Successful fertilization and pregnancy outcome in in-vitro fertilization using cryopreserved/thawed spermatozoa from patients with malignant diseases. 
  • Successful in vivo recovery and extended storage of additive solution (AS)-5 red blood cells after deglycerolization and resuspension in AS-3 for 15 days with an automated closed system. 
  • Synthetic estrogens suppress granulosa cell progesterone production in vitro. 
  • The Center for HIV/AIDS Vaccine Immunology (CHAVI) multi-site quality assurance program for cryopreserved human peripheral blood mononuclear cells. 
  • The Cord Blood Apgar: a novel scoring system to optimize selection of banked cord blood grafts for transplantation (CME). 
  • The biology and ethics of banking fetal liver hematopoietic stem cells for in utero transplantation. 
  • The corneal epithelium after optisol-GS storage. 
  • The human thymic microenvironment during organ culture. 
  • Transfer of cryopreserved-thawed pre-embryos in a cycle using exogenous steroids without prior gonadotrophin-releasing hormone agonist suppression yields favourable pregnancy results. 
  • Transfer of cryopreserved-thawed pre-embryos in a natural cycle or a programmed cycle with exogenous hormonal replacement yields similar pregnancy results. 
  • Transplantation of cord-blood cells. 
  • Two-phase shelf-life estimation. 
  • Umbilical cord blood: a novel alternative source of hematopoietic stem cells for bone marrow transplantation. 
  • Variability in the fibrinogen and von Willebrand factor content of cryoprecipitate. Implications for reducing donor exposure. 
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  Keywords of People

  • Ferrari, Guido, Associate Professor of Surgery, Molecular Genetics and Microbiology