Microscopy, Immunoelectron

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  Subject Areas on Research

  • A single amino acid change in the cytoplasmic domain of the simian immunodeficiency virus transmembrane molecule increases envelope glycoprotein expression on infected cells. 
  • Alpha-internexin is structurally and functionally associated with the neurofilament triplet proteins in the mature CNS. 
  • Anti-intercellular adhesion molecule-1 antibody and intercellular adhesion molecule-1 gene deficiency do not prevent pulmonary neutrophil recruitment in polymicrobial sepsis. 
  • Apolipoprotein E is localized to the cytoplasm of human cortical neurons: a light and electron microscopic study. 
  • Association of whirlin with Cav1.3 (alpha1D) channels in photoreceptors, defining a novel member of the usher protein network. 
  • Astrocytes refine cortical connectivity at dendritic spines. 
  • Beta ig-h3 is synthesized by corneal epithelium and perhaps endotheliumin Fuchs' dystrophic corneas. 
  • Beta-arrestin2, a novel member of the arrestin/beta-arrestin gene family. 
  • Characterization of a novel family of ciliary body glycoproteins. 
  • Clustering of voltage-sensitive sodium channels on axons is independent of direct Schwann cell contact in the dystrophic mouse. 
  • Concentric intermediate filament lattice links to specialized Z-band junctional complexes in sonic muscle fibers of the type I male midshipman fish. 
  • Concerted use of immunologic and ultrastructural analyses in diagnostic medicine: immunoelectron microscopy and correlative microscopy. 
  • Contrasting response of lung parenchymal cells to instilled TNF alpha and IFN gamma: the inducibility of specific cell ICAM-1 in vivo. 
  • Differential expression of muscarinic acetylcholine receptors across excitatory and inhibitory cells in visual cortical areas V1 and V2 of the macaque monkey. 
  • Dynamic changes in the distribution of cytoplasmic myosin during Drosophila embryogenesis. 
  • Electron immunocytochemical analysis of posterior hyaloid associated with diabetic macular edema. 
  • Endocytosis and synaptic removal of NR3A-containing NMDA receptors by PACSIN1/syndapin1. 
  • Evidence for astrocytes as a potential source of the glutamate excess in temporal lobe epilepsy. 
  • Evidence that down-regulation of beta-cell glucose transporters in non-insulin-dependent diabetes may be the cause of diabetic hyperglycemia. 
  • Expression of a copper-containing amine oxidase by human ciliary body. 
  • Expression of a mitochondrial progesterone receptor in human spermatozoa correlates with a progestin-dependent increase in mitochondrial membrane potential. 
  • Expression of a variant form of the glutamate transporter GLT1 in neuronal cultures and in neurons and astrocytes in the rat brain. 
  • GFAP is necessary for the integrity of CNS white matter architecture and long-term maintenance of myelination. 
  • Gold/Fab immuno electron microscopy localization of troponin H and troponin T in Lethocerus flight muscle. 
  • IgA-binding structures in dermatitis herpetiformis skin are independent of elastic-microfibrillar bundles. 
  • Immunolocalization of beta ig-h3 protein in 5q31-linked corneal dystrophies and normal corneas. 
  • Immunolocalization of beta-D-glucans, pectins, and arabinogalactan-proteins during intrusive growth and elongation of nonarticulated laticifers in Asclepias speciosa Torr. 
  • Immunological detection of the mitochondrial F1-ATPase alpha subunit in the matrix of rat liver peroxisomes. A protein involved in organelle biogenesis? 
  • Inability of encapsulated Klebsiella pneumoniae to assemble functional type 1 fimbriae on their surface. 
  • Increased amphetamine-induced hyperactivity and reward in mice overexpressing the dopamine transporter. 
  • Increased expression of phosphate-activated glutaminase in hippocampal neurons in human mesial temporal lobe epilepsy. 
  • International Bullous Diseases Group: consensus on diagnostic criteria for epidermolysis bullosa acquisita. 
  • Involvement of cellular caveolae in bacterial entry into mast cells. 
  • Involvement of dynamin-2 in formation of discoid vesicles in urinary bladder umbrella cells. 
  • Islet amyloid formation associated with hyperglycemia in transgenic mice with pancreatic beta cell expression of human islet amyloid polypeptide. 
  • Leptin Is Produced by Parathyroid Glands and Stimulates Parathyroid Hormone Secretion. 
  • Ligand-dependent recruitment of the ErbB4 signaling complex into neuronal lipid rafts. 
  • Localization of a baculovirus-induced chitinase in the insect cell endoplasmic reticulum. 
  • Location of the PH-20 protein on acrosome-intact and acrosome-reacted spermatozoa of cynomolgus macaques. 
  • Mast cell activation by Mycobacterium tuberculosis: mediator release and role of CD48. 
  • Molecular and biochemical characterization of folate transport proteins in retinal Müller cells. 
  • Molecular genetic analysis of two human sperm fibrous sheath proteins, AKAP4 and AKAP3, in men with dysplasia of the fibrous sheath. 
  • Nonspecific interstitial pneumonia, alveolar proteinosis, and abnormal proprotein trafficking resulting from a spontaneous mutation in the surfactant protein C gene. 
  • Nuclear interaction of EGFR and STAT3 in the activation of the iNOS/NO pathway. 
  • Ocular nanoparticle toxicity and transfection of the retina and retinal pigment epithelium. 
  • Pcp1p, an Spc110p-related calmodulin target at the centrosome of the fission yeast Schizosaccharomyces pombe. 
  • Platelets contain releasable coagulation factor IX antigen. 
  • Platelets contain releasable coagulation factor IX antigen: response. 
  • Polarized secretory trafficking directs cargo for asymmetric dendrite growth and morphogenesis. 
  • Rapid endocytosis of a G protein-coupled receptor: substance P evoked internalization of its receptor in the rat striatum in vivo. 
  • Regulation of fibrinolysis by platelet-released plasminogen activator inhibitor 1: light scattering and ultrastructural examination of lysis of a model platelet-fibrin thrombus. 
  • SAMP14, a novel, acrosomal membrane-associated, glycosylphosphatidylinositol-anchored member of the Ly-6/urokinase-type plasminogen activator receptor superfamily with a role in sperm-egg interaction. 
  • SP75 is encoded by the DP87 gene and belongs to a family of modular Dictyostelium discoideum outer layer spore coat proteins. 
  • Secretion of the Haemophilus influenzae HMW1 and HMW2 adhesins involves a periplasmic intermediate and requires the HMWB and HMWC proteins. 
  • Subcellular partitioning of MRP RNA assessed by ultrastructural and biochemical analysis. 
  • Synaptic relationship between substance P and the substance P receptor: light and electron microscopic characterization of the mismatch between neuropeptides and their receptors. 
  • Tenascin-C in rat lung: distribution, ontogeny and role in branching morphogenesis. 
  • The G protein-coupled receptor kinase 2 is a microtubule-associated protein kinase that phosphorylates tubulin. 
  • The Haemophilus influenzae Type b hcsA and hcsB gene products facilitate transport of capsular polysaccharide across the outer membrane and are essential for virulence. 
  • The angiotensin II type I receptor-associated protein, ATRAP, is a transmembrane protein and a modulator of angiotensin II signaling. 
  • The aquaporins, blueprints for cellular plumbing systems. 
  • The candidate sour taste receptor, PKD2L1, is expressed by type III taste cells in the mouse. 
  • The inducibly expressed GTPase localizes to the endoplasmic reticulum, independently of GTP binding. 
  • The lysosomal granule membrane protein, LAMP-2, is also present in platelet dense granule membranes. 
  • Ultrastructural Analysis of Vesicular Transport in Electrotransfection. 
  • Ultrastructural localization of variant forms of cystic fibrosis transmembrane conductance regulator in human bronchial epithelial of xenografts. 
  • Visualization of slow axonal transport in vivo. 
  • ePAD, an oocyte and early embryo-abundant peptidylarginine deiminase-like protein that localizes to egg cytoplasmic sheets. 
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  Keywords of People

  • Bennett, Vann, George B. Geller Distinguished Professor for Research in Molecular Biology in the School of Medicine, Duke Cancer Institute
  • Erickson, Harold Paul, James B. Duke Distinguished Professor of Cell Biology, Biomedical Engineering
  • Taylor, Gregory Alan, Associate Professor in Medicine, Immunology