Mutagenesis, Site-Directed

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  Subject Areas on Research

  • A High-Throughput Mutational Scan of an Intrinsically Disordered Acidic Transcriptional Activation Domain. 
  • A Novel Phosphoregulatory Switch Controls the Activity and Function of the Major Catalytic Subunit of Protein Kinase A in Aspergillus fumigatus. 
  • A central role for beta-arrestins and clathrin-coated vesicle-mediated endocytosis in beta2-adrenergic receptor resensitization. Differential regulation of receptor resensitization in two distinct cell types. 
  • A cis-acting sequence homologous to the yeast filamentation and invasion response element regulates expression of a pectinase gene from the bean pathogen Colletotrichum lindemuthianum. 
  • A cooperative interaction between NF-kappa B and Sp1 is required for HIV-1 enhancer activation. 
  • A critical role of two positively charged amino acids in the Jas motif of Arabidopsis JAZ proteins in mediating coronatine- and jasmonoyl isoleucine-dependent interactions with the COI1 F-box protein. 
  • A functional variant at the miR-184 binding site in TNFAIP2 and risk of squamous cell carcinoma of the head and neck. 
  • A highly conserved tyrosine residue in G protein-coupled receptors is required for agonist-mediated beta 2-adrenergic receptor sequestration. 
  • A mutant PTH/PTHrP type I receptor in enchondromatosis. 
  • A new activity of doublecortin in recognition of the phospho-FIGQY tyrosine in the cytoplasmic domain of neurofascin. 
  • A non-oncogenic HPV 16 E6/E7 vaccine enhances treatment of HPV expressing tumors. 
  • A novel relaxase homologue is involved in chromosomal DNA processing for type IV secretion in Neisseria gonorrhoeae. 
  • A pH-sensitive RNA tertiary interaction affects self-cleavage activity of the HDV ribozymes in the absence of added divalent metal ion. 
  • A single amino acid change in the cytoplasmic domain of the simian immunodeficiency virus transmembrane molecule increases envelope glycoprotein expression on infected cells. 
  • A structural pathway for activation of the kinesin motor ATPase. 
  • A substrate specific functional polymorphism of human gamma-glutamyl hydrolase alters catalytic activity and methotrexate polyglutamate accumulation in acute lymphoblastic leukaemia cells. 
  • A thermodynamic ligand binding study of the third PDZ domain (PDZ3) from the mammalian neuronal protein PSD-95. 
  • A transforming growth factor beta type I receptor that signals to activate gene expression. 
  • A unique phosphorylation-dependent mechanism for the activation of Ca2+/calmodulin-dependent protein kinase type IV/GR. 
  • Activation of Cardiac Fibroblast Growth Factor Receptor 4 Causes Left Ventricular Hypertrophy. 
  • Activation of four enzymes by two series of calmodulin mutants with point mutations in individual Ca2+ binding sites. 
  • Activation-dependent subconductance levels in the drk1 K channel suggest a subunit basis for ion permeation and gating. 
  • Adaptive Evolution of the GDH2 Allosteric Domain Promotes Gliomagenesis by Resolving IDH1R132H-Induced Metabolic Liabilities. 
  • Adding a positive charge at residue 46 of Drosophila alcohol dehydrogenase increases cofactor specificity for NADP+. 
  • Allosteric activation of proto-oncogene kinase Src by GPCR-beta-arrestin complexes. 
  • Allosteric regulation of type I hexokinase: A site-directed mutational study indicating location of the functional glucose 6-phosphate binding site in the N-terminal half of the enzyme. 
  • Alternative translation initiation site usage results in two structurally distinct forms of Pit-1. 
  • Amino acid residues in thrombin-sensitive region and first epidermal growth factor domain of vitamin K-dependent protein S determining specificity of the activated protein C cofactor function. 
  • Amino acid substitutions in the Dictyostelium G alpha subunit G alpha 2 produce dominant negative phenotypes and inhibit the activation of adenylyl cyclase, guanylyl cyclase, and phospholipase C. 
  • Amyloidogenic and anti-amyloidogenic properties of recombinant transthyretin variants. 
  • An MYH9 human disease model in flies: site-directed mutagenesis of the Drosophila non-muscle myosin II results in hypomorphic alleles with dominant character. 
  • An active site tyrosine influences the ability of the dimethyl sulfoxide reductase family of molybdopterin enzymes to reduce S-oxides. 
  • An alanine in segment 3 of domain III (IIIS3) of the cockroach sodium channel contributes to the low pyrethroid sensitivity of an alternative splice variant. 
  • An alanine residue in the M3-M4 linker lines the glycine binding pocket of the N-methyl-D-aspartate receptor. 
  • Analysis of the neurofibromatosis type 1 (NF1) GAP-related domain by site-directed mutagenesis. 
  • Analysis of trans-acting response decoy RNA-mediated inhibition of human immunodeficiency virus type 1 transactivation. 
  • Animal model for maturity-onset diabetes of the young generated by disruption of the mouse glucokinase gene. 
  • Apoptosis: final common pathway of photoreceptor death in rd, rds, and rhodopsin mutant mice. 
  • Arsenic trioxide uptake by human and rat aquaglyceroporins. 
  • Assessment of disparate structural features in three models of the hepatitis delta virus ribozyme. 
  • Atomic distance estimates from disulfides and high-affinity metal-binding sites in a K+ channel pore. 
  • Autoimmune epitopes in messenger RNA. 
  • Autoinhibition of casein kinase I epsilon (CKI epsilon) is relieved by protein phosphatases and limited proteolysis. 
  • BCR-ABL-induced oncogenesis is mediated by direct interaction with the SH2 domain of the GRB-2 adaptor protein. 
  • Beta-adrenergic receptor sequestration. A potential mechanism of receptor resensitization. 
  • C-terminal regions of the human telomerase catalytic subunit essential for in vivo enzyme activity. 
  • CCM1 and CCM2 protein interactions in cell signaling: implications for cerebral cavernous malformations pathogenesis. 
  • CD4 mimetics sensitize HIV-1-infected cells to ADCC. 
  • Ca2+-sensitive inactivation and facilitation of L-type Ca2+ channels both depend on specific amino acid residues in a consensus calmodulin-binding motif in the(alpha)1C subunit. 
  • Calcineurin mutants render T lymphocytes resistant to cyclosporin A. 
  • Calcineurin-binding protein Cbp1 directs the specificity of calcineurin-dependent hyphal elongation during mating in Cryptococcus neoformans. 
  • Calcineurin-dependent dephosphorylation of the transcription factor CrzA at specific sites controls conidiation, stress tolerance, and virulence of Aspergillus fumigatus. 
  • Calmodulin kinase II chimeras used to investigate the structural requirements for smooth muscle myosin light chain kinase autoinhibition and calmodulin-dependent activation. 
  • Calmodulin supports both inactivation and facilitation of L-type calcium channels. 
  • Calspermin gene transcription is regulated by two cyclic AMP response elements contained in an alternative promoter in the calmodulin kinase IV gene. 
  • Caspase-8-mediated PAR-4 cleavage is required for TNFα-induced apoptosis. 
  • Caspase-mediated cleavage of HuR in the cytoplasm contributes to pp32/PHAP-I regulation of apoptosis. 
  • Catalytic activity is required for calcium/calmodulin-dependent protein kinase IV to enter the nucleus. 
  • Cellular mechanisms regulating protein phosphatase-1. A key functional interaction between inhibitor-2 and the type 1 protein phosphatase catalytic subunit. 
  • Characterization and regulation of the trehalose synthesis pathway and its importance in the pathogenicity of Cryptococcus neoformans. 
  • Characterization of Escherichia coli MoeB and its involvement in the activation of molybdopterin synthase for the biosynthesis of the molybdenum cofactor. 
  • Characterization of a murine monoclonal antibody that mimics heparin-induced thrombocytopenia antibodies. 
  • Characterization of a putative insulin-responsive element and its binding protein(s) in rat angiotensinogen gene promoter: regulation by glucose and insulin. 
  • Characterization of an iron-sulfur cluster assembly protein (ISU1) from Schizosaccharomyces pombe. 
  • Characterization of cDNA encoding mouse DNA repair protein O6-methylguanine-DNA methyltransferase and high-level expression of the wild-type and mutant proteins in Escherichia coli. 
  • Characterization of key residues in the subdomain encoded by exons 8 and 9 of human inducible nitric oxide synthase: a critical role for Asp-280 in substrate binding and subunit interactions. 
  • Characterization of proteins that interact with the cell-cycle regulatory protein Ran/TC4. 
  • Characterization of substrate phosphorylation and use of calmodulin mutants to address implications from the enzyme crystal structure of calmodulin-dependent protein kinase I. 
  • Characterization of the Adeno-Associated Virus 1 and 6 Sialic Acid Binding Site. 
  • Chemical modification and site-directed mutagenesis of conserved HXXH and PP-loop motif arginines and histidines in the murine bifunctional ATP sulfurylase/adenosine 5'-phosphosulfate kinase. 
  • Chicken progesterone receptor expressed in Saccharomyces cerevisiae is correctly phosphorylated at all four Ser-Pro phosphorylation sites. 
  • Clamp subunit dissociation dictates bacteriophage T4 DNA polymerase holoenzyme disassembly. 
  • Cleavage of oligoribonucleotides by a ribozyme derived from the hepatitis delta virus RNA sequence. 
  • Cloning of the promoter region of human endoglin, the target gene for hereditary hemorrhagic telangiectasia type 1. 
  • Clustered charged amino acids of human adenosine deaminase comprise a functional epitope for binding the adenosine deaminase complexing protein CD26/dipeptidyl peptidase IV. 
  • Cocrystal structure of protein farnesyltransferase complexed with a farnesyl diphosphate substrate. 
  • Comparative analysis of type 2b von Willebrand disease mutations: implications for the mechanism of von Willebrand factor binding to platelets. 
  • Comparison of myristoyl-CoA:protein N-myristoyltransferases from three pathogenic fungi: Cryptococcus neoformans, Histoplasma capsulatum, and Candida albicans. 
  • Comparison of the functional differences for the homologous residues within the carboxy phosphate and carbamate domains of carbamoyl phosphate synthetase. 
  • Confirmation of the involvement of protein domain movement during the catalytic cycle of the cytochrome bc1 complex by the formation of an intersubunit disulfide bond between cytochrome b and the iron-sulfur protein. 
  • Conformation-assisted inhibition of protein-tyrosine phosphatase-1B elicits inhibitor selectivity over T-cell protein-tyrosine phosphatase. 
  • Conformational changes in the photocycle of Anabaena sensory rhodopsin: absence of the Schiff base counterion protonation signal. 
  • Conserved Residues Control Activation of Mammalian G Protein-Coupled Odorant Receptors. 
  • Conserved motifs II to VI of DNA helicase II from Escherichia coli are all required for biological activity. 
  • Constitutive activation of the N-methyl-D-aspartate receptor via cleft-spanning disulfide bonds. 
  • Constitutive activation of the alpha 1B-adrenergic receptor by all amino acid substitutions at a single site. Evidence for a region which constrains receptor activation. 
  • Construction of a catalytically active iron superoxide dismutase by rational protein design. 
  • Construction of a family of Cys2His2 zinc binding sites in the hydrophobic core of thioredoxin by structure-based design. 
  • Construction of a novel redox protein by rational design: conversion of a disulfide bridge into a mononuclear iron-sulfur center. 
  • Construction of new ligand binding sites in proteins of known structure. II. Grafting of a buried transition metal binding site into Escherichia coli thioredoxin. 
  • Contribution of a buried hydrogen bond to lambda repressor folding kinetics. 
  • Contributions of Asn2198, Met2199, and Phe2200 in the factor VIII C2 domain to cofactor activity, phospholipid-binding, and von Willebrand factor-binding. 
  • Control of a biomolecular motor-powered nanodevice with an engineered chemical switch. 
  • Control of protein-ligand recognition using a stimuli-responsive polymer. 
  • Conversion of Tyr361 beta to Leu in mammalian protein farnesyltransferase impairs product release but not substrate recognition. 
  • Conversion of protein phosphatase 1 catalytic subunit to a Mn(2+)-dependent enzyme impairs its regulation by inhibitor 1. 
  • Copper-dependent site-specific mutagenesis by benzoyl peroxide in the supF gene of the mutation reporter plasmid pS189. 
  • Core sequences and a cleavage site wobble pair required for HDV antigenomic ribozyme self-cleavage. 
  • Coupling of proton binding in extracellular domain to channel gating in acid-sensing ion channel. 
  • Crucial role for the VWF A1 domain in binding to type IV collagen. 
  • Crystal structure of the gephyrin-related molybdenum cofactor biosynthesis protein MogA from Escherichia coli. 
  • Crystal structures of the Klenow fragment of DNA polymerase I complexed with deoxynucleoside triphosphate and pyrophosphate. 
  • Crystallization of a fragment of human fibronectin: introduction of methionine by site-directed mutagenesis to allow phasing via selenomethionine. 
  • Deactylase inhibitors disrupt cellular complexes containing protein phosphatases and deacetylases. 
  • Decreased tumorigenesis in mice with a Kras point mutation at C118. 
  • Defective IL7R expression in T(-)B(+)NK(+) severe combined immunodeficiency. 
  • Defining a two-pronged structural model for PB1 (Phox/Bem1p) domain interaction in plant auxin responses. 
  • Defining fibronectin's cell adhesion synergy site by site-directed mutagenesis. 
  • Deletion and site-directed mutagenesis of the ATP-binding motif (P-loop) in the bifunctional murine ATP-sulfurylase/adenosine 5'-phosphosulfate kinase enzyme. 
  • Deletion of V335 from the L2 domain of the insulin receptor results in a conformationally abnormal receptor that is unable to bind insulin and causes Donohue's syndrome in a human subject. 
  • Deletion of cysteine 369 in lysyl hydroxylase 1 eliminates enzyme activity and causes Ehlers-Danlos syndrome type VI. 
  • Deletion of presenilin 1 hydrophilic loop sequence leads to impaired gamma-secretase activity and exacerbated amyloid pathology. 
  • Derivation of a biologically contained replication system for human immunodeficiency virus type 1. 
  • Design, expression, and immunogenicity of a soluble HIV trimeric envelope fragment adopting a prefusion gp41 configuration. 
  • Development of mammalian serum albumin affinity purification media by peptide phage display. 
  • Different structural changes occur in blue- and green-proteorhodopsins during the primary photoreaction. 
  • Differential regulation of dopamine D1A receptor responsiveness by various G protein-coupled receptor kinases. 
  • Dilated cardiomyopathy in transgenic mice expressing a mutant A subunit of protein phosphatase 2A. 
  • Directed evolution of a new catalytic site in 2-keto-3-deoxy-6-phosphogluconate aldolase from Escherichia coli. 
  • Directed evolution of a pyruvate aldolase to recognize a long chain acyl substrate. 
  • Discrimination of tRNALeu isoacceptors by the insertion mutant of Escherichia coli leucyl-tRNA synthetase. 
  • Disease-causing mutations in exon 11 of the medium-chain acyl-CoA dehydrogenase gene. 
  • Distinct conformational changes in beta-arrestin report biased agonism at seven-transmembrane receptors. 
  • Distinct single amino acid replacements in the control of virulence regulator protein differentially impact streptococcal pathogenesis. 
  • Domain unfolding plays a role in superfibronectin formation. 
  • Ecdysone-inducible gene expression in mammalian cells and transgenic mice. 
  • Echinomycin, a bis-intercalating agent, induces C-->T mutations via cytosine deamination. 
  • Effect of a dominant negative ras on myocardial hypertrophy by using adenoviral-mediated gene transfer. 
  • Effect of cysteine residues on the activity of arginyl-tRNA synthetase from Escherichia coli. 
  • Effect of famoxadone on photoinduced electron transfer between the iron-sulfur center and cytochrome c1 in the cytochrome bc1 complex. 
  • Effect of streptavidin RGD mutant on the adhesion of endothelial cells. 
  • Effect of the laccase gene CNLAC1, on virulence of Cryptococcus neoformans. 
  • Effects of the NIK aly mutation on NF-kappaB activation by the Epstein-Barr virus latent infection membrane protein, lymphotoxin beta receptor, and CD40. 
  • Elevated blood pressure and enhanced myocardial contractility in mice with severe IGF-1 deficiency. 
  • Engineered chimeric streptavidin tetramers as novel tools for bioseparations and drug delivery. 
  • Enhanced neonatal Fc receptor function improves protection against primate SHIV infection. 
  • Escherichia coli purine repressor: key residues for the allosteric transition between active and inactive conformations and for interdomain signaling. 
  • Essential role of conserved arginine 160 in intramolecular electron transfer in human sulfite oxidase. 
  • Evidence for an electrostatic mechanism of force generation by the bacteriophage T4 DNA packaging motor. 
  • Experimental evidence for the secondary structure of the hepatitis delta virus ribozyme. 
  • Experimental validation of the docking orientation of Cdc25 with its Cdk2-CycA protein substrate. 
  • Expression and site-directed mutagenesis of the phosphatidylcholine-preferring phospholipase C of Bacillus cereus: probing the role of the active site Glu146. 
  • Expression of a constitutively active Ca2+/calmodulin-dependent kinase in Aspergillus nidulans spores prevents germination and entry into the cell cycle. 
  • Facilitative glucose transporter Glut1 is actively excluded from rod outer segments. 
  • Factor VIII Arg2304 --> His binds to phosphatidylserine and is a functional cofactor in the factor X-ase complex. 
  • Fine epitope signature of antibody neutralization breadth at the HIV-1 envelope CD4-binding site. 
  • Folding kinetics of a fluorescent variant of monomeric lambda repressor. 
  • Founder's Award, Society for Biomaterials. Sixth World Biomaterials Congress 2000, Kamuela, HI,May 15-20, 2000. Really smart bioconjugates of smart polymers and receptor proteins. 
  • Functional analysis of the interface between the tandem C2 domains of synaptotagmin-1. 
  • Functional asymmetry of transmembrane segments in nicotinic acetylcholine receptors. 
  • Functional characterization of a unique liver gene promoter. 
  • Functional evolution of an anthocyanin pathway enzyme during a flower color transition. 
  • Functional expression of an arachnid sodium channel reveals residues responsible for tetrodotoxin resistance in invertebrate sodium channels. 
  • Functional replacement of the mouse E2A gene with a human HEB cDNA. 
  • Further insights into quinone cofactor biogenesis: probing the role of mauG in methylamine dehydrogenase tryptophan tryptophylquinone formation. 
  • G protein-coupled receptor kinase 2 activates radixin, regulating membrane protrusion and motility in epithelial cells. 
  • Gamma-globin gene promoter elements required for interaction with globin enhancers. 
  • Generation of random mutant libraries with multiple primers in a single reaction. 
  • Glutamate 350 Plays an Essential Role in Conformational Gating of Long-Range Radical Transport in Escherichia coli Class Ia Ribonucleotide Reductase. 
  • Green fluorescent protein as a tag for molecular motor proteins. 
  • HDAC6 modulates cell motility by altering the acetylation level of cortactin. 
  • Hepatocyte nuclear factor-4alpha mediates redox sensitivity of inducible nitric-oxide synthase gene transcription. 
  • Human catechol-O-methyltransferase haplotypes modulate protein expression by altering mRNA secondary structure. 
  • Human substance P receptor lacking the C-terminal domain remains competent to desensitize and internalize. 
  • Human sulfite oxidase R160Q: identification of the mutation in a sulfite oxidase-deficient patient and expression and characterization of the mutant enzyme. 
  • Identification and cloning of a negative regulator of systemic acquired resistance, SNI1, through a screen for suppressors of npr1-1 
  • Identification of a cluster of residues in transmembrane segment 6 of domain III of the cockroach sodium channel essential for the action of pyrethroid insecticides. 
  • Identification of a cysteine residue essential for activity of protein farnesyltransferase. Cys299 is exposed only upon removal of zinc from the enzyme. 
  • Identification of a mutant DNA polymerase delta in Saccharomyces cerevisiae with an antimutator phenotype for frameshift mutations. 
  • Identification of a negative regulatory surface within estrogen receptor alpha provides evidence in support of a role for corepressors in regulating cellular responses to agonists and antagonists. 
  • Identification of a new family of protein phosphatase 2A regulatory subunits. 
  • Identification of a residue critical for maintaining the functional conformation of BPTI. 
  • Identification of amino acid residues involved in structural and ubiquinone-binding functions of subunit IV of the cytochrome bc1 complex from Rhodobacter sphaeroides. 
  • Identification of amino acids essential for calmodulin binding and activation of smooth muscle myosin light chain kinase. 
  • Identification of gemin5 as a novel 7-methylguanosine cap-binding protein. 
  • Identification of inhibitory autophosphorylation sites in casein kinase I epsilon. 
  • Identification of key amino acids responsible for the substantially higher affinities of human type 1 3beta-hydroxysteroid dehydrogenase/isomerase (3beta-HSD1) for substrates, coenzymes, and inhibitors relative to human 3beta-HSD2. 
  • Identification of residues in alpha-macroglobulins involved in activation of the alpha 2-macroglobulin signaling receptor. 
  • Identification of the TBX5 transactivating domain and the nuclear localization signal. 
  • Immunochemical and molecular analysis of antigen binding to lipid anchored and soluble forms of an MHC independent human alpha/beta T cell receptor. 
  • Immunogenicity of membrane-bound HIV-1 gp41 membrane-proximal external region (MPER) segments is dominated by residue accessibility and modulated by stereochemistry. 
  • Immunohistochemical evidence of seizure-induced activation of trkB receptors in the mossy fiber pathway of adult mouse hippocampus. 
  • Immunotoxins that target an oncogenic mutant epidermal growth factor receptor expressed in human tumors. 
  • Immunotoxins with increased activity against epidermal growth factor receptor vIII-expressing cells produced by antibody phage display. 
  • Implications for proteasome nuclear localization revealed by the structure of the nuclear proteasome tether protein Cut8. 
  • Improved antitumor activity of a recombinant anti-Lewis(y) immunotoxin not requiring proteolytic activation. 
  • Improving upon nature: active site remodeling produces highly efficient aldolase activity toward hydrophobic electrophilic substrates. 
  • In cardiomyocyte hypoxia, insulin-like growth factor-I-induced antiapoptotic signaling requires phosphatidylinositol-3-OH-kinase-dependent and mitogen-activated protein kinase-dependent activation of the transcription factor cAMP response element-binding protein. 
  • In vitro polymerization of tau protein monitored by laser light scattering: method and application to the study of FTDP-17 mutants. 
  • In vitro selection and characterization of human immunodeficiency virus type 2 with decreased susceptibility to lopinavir. 
  • In vivo post-translational processing and subunit reconstitution of cephalosporin acylase from Pseudomonas sp. 130. 
  • Influence of Arginines 93, 97, and 101 of thrombin to its functional specificity. 
  • Inhibitor-kappaB kinase-beta regulates LPS-induced TNF-alpha production in cardiac myocytes through modulation of NF-kappaB p65 subunit phosphorylation. 
  • Integrating energy calculations with functional assays to decipher the specificity of G protein-RGS protein interactions. 
  • Interacting targets of the farnesyl of transducin gamma-subunit. 
  • Interaction of NPR1 with basic leucine zipper protein transcription factors that bind sequences required for salicylic acid induction of the PR-1 gene. 
  • Interaction of the regulatory subunit (RII) of cAMP-dependent protein kinase with RII-anchoring proteins occurs through an amphipathic helix binding motif. 
  • Internalization of fibroblast growth factor receptor is inhibited by a point mutation at tyrosine 766. 
  • Intestinal tumorigenesis is suppressed in mice lacking the metalloproteinase matrilysin. 
  • Intracellular pH regulation by Na(+)/H(+) exchange requires phosphatidylinositol 4,5-bisphosphate. 
  • Intrasteric regulation of myosin light chain kinase: the pseudosubstrate prototope binds to the active site. 
  • Intronic cis-regulatory modules mediate tissue-specific and microbial control of angptl4/fiaf transcription. 
  • Investigating the SecY plug movement at the SecYEG translocation channel. 
  • Isoprenylation of a protein kinase. Requirement of farnesylation/alpha-carboxyl methylation for full enzymatic activity of rhodopsin kinase. 
  • K channel subconductance levels result from heteromeric pore conformations. 
  • Kinetic analysis of the zinc-dependent deacetylase in the lipid A biosynthetic pathway. 
  • Kinetic analysis of zinc ligand mutants of mammalian protein farnesyltransferase. 
  • Kinetic role of helix caps in protein folding is context-dependent. 
  • Kinetic studies of protein farnesyltransferase mutants establish active substrate conformation. 
  • LFA-1 binding site in ICAM-3 contains a conserved motif and non-contiguous amino acids. 
  • Late onset N-acetylglutamate synthase deficiency caused by hypomorphic alleles. 
  • Lefty proteins exhibit unique processing and activate the MAPK pathway. 
  • Localization of the activation gate for small conductance Ca2+-activated K+ channels. 
  • Long range dynamic effects of point-mutations trap a response regulator in an active conformation. 
  • Lysine(164)alpha of protein farnesyltransferase is important for both CaaX substrate binding and catalysis. 
  • Maintenance of G2 arrest in the Xenopus oocyte: a role for 14-3-3-mediated inhibition of Cdc25 nuclear import. 
  • Malignant breast epithelial cells stimulate aromatase expression via promoter II in human adipose fibroblasts: an epithelial-stromal interaction in breast tumors mediated by CCAAT/enhancer binding protein beta. 
  • Mapping staphylococcal nuclease conformation using an EDTA-Fe derivative attached to genetically engineered cysteine residues. 
  • Mechanism of neutralization by the broadly neutralizing HIV-1 monoclonal antibody VRC01. 
  • Mechanism of pyranopterin ring formation in molybdenum cofactor biosynthesis. 
  • Mechanistic studies of human molybdopterin synthase reaction and characterization of mutants identified in group B patients of molybdenum cofactor deficiency. 
  • Methionine to glutamine substitutions in the C-terminal domain of calmodulin impair the activation of three protein kinases. 
  • Micro-RNA-181a regulates osteopontin-dependent metastatic function in hepatocellular cancer cell lines. 
  • MicroRNA-182 and microRNA-200a control G-protein subunit α-13 (GNA13) expression and cell invasion synergistically in prostate cancer cells. 
  • Minimal requirement of tyrosine residues of linker for activation of T cells in TCR signaling and thymocyte development. 
  • Mitochondrial phosphatase PTPMT1 is essential for cardiolipin biosynthesis. 
  • Modeling of sequestration and down regulation in cells containing beta2-adrenergic receptors. 
  • Modulation of ligand binding affinity of the adipocyte lipid-binding protein by selective mutation. Analysis in vitro and in situ. 
  • Molecular analysis of mutations in a patient with purine nucleoside phosphorylase deficiency. 
  • Molecular analysis of the Cryptococcus neoformans ADE2 gene, a selectable marker for transformation and gene disruption. 
  • Molecular basis for the enterocyte tropism exhibited by Salmonella typhimurium type 1 fimbriae. 
  • Molecular cloning of dimethyl sulfoxide reductase from Rhodobacter sphaeroides. 
  • Molecular determinants on the insect sodium channel for the specific action of type II pyrethroid insecticides. 
  • Molecular determinants underlying the formation of stable intracellular G protein-coupled receptor-beta-arrestin complexes after receptor endocytosis*. 
  • Molecular dissection of gating in the ClC-2 chloride channel. 
  • Molecular evidence for dual pyrethroid-receptor sites on a mosquito sodium channel. 
  • Molecular mechanism of activation of human musk receptors OR5AN1 and OR1A1 by (R)-muscone and diverse other musk-smelling compounds. 
  • Molecular mechanism of tissue-specific regulation of mouse renin gene expression by cAMP. Identification of an inhibitory protein that binds nuclear transcriptional factor. 
  • Molecular recognition of ketamine by a subset of olfactory G protein-coupled receptors. 
  • Molecular structure and function of the novel BrnT/BrnA toxin-antitoxin system of Brucella abortus. 
  • Most mutations that cause spinocerebellar ataxia autosomal recessive type 16 (SCAR16) destabilize the protein quality-control E3 ligase CHIP. 
  • Multiple Surface Regions on the Niemann-Pick C2 Protein Facilitate Intracellular Cholesterol Transport. 
  • Multiple gene deletion in Cryptococcus neoformans using the Cre-lox system. 
  • Multiple structural elements define the specificity of recombinant human inhibitor-1 as a protein phosphatase-1 inhibitor. 
  • Mutagenesis by exocyclic alkylamino purine adducts in Escherichia coli. 
  • Mutagenesis of the phosphate-binding pocket of KDPG aldolase enhances selectivity for hydrophobic substrates. 
  • Mutagenic analysis of functional domains of the mos proto-oncogene and identification of the sites important for MAPK activation and DNA binding. 
  • Mutants of the EcoRI endonuclease with promiscuous substrate specificity implicate residues involved in substrate recognition. 
  • Mutational Analysis of Measles Virus Suggests Constraints on Antigenic Variation of the Glycoproteins. 
  • Mutational analysis of Gbetagamma and phospholipid interaction with G protein-coupled receptor kinase 2. 
  • Mutational analysis of active site residues in the Staphylococcus aureus transpeptidase SrtA. 
  • Mutational analysis of potential zinc-binding residues in the active site of the enterococcal D-Ala-D-Ala dipeptidase VanX. 
  • Mutational analysis of state-dependent contacts in the pore module of eukaryotic sodium channels. 
  • Mutational analysis of the autoinhibitory domain of calmodulin kinase II. 
  • Mutational analysis of the fractalkine chemokine domain. Basic amino acid residues differentially contribute to CX3CR1 binding, signaling, and cell adhesion. 
  • Mutational analysis of the function of Met137 and Ile197, two amino acids implicated in sequence-specific DNA recognition by the EcoRI endonuclease. 
  • Mutations in the glucose-6-phosphatase gene are associated with glycogen storage disease types 1a and 1aSP but not 1b and 1c. 
  • Mutations that Separate the Functions of the Proofreading Subunit of the Escherichia coli Replicase. 
  • Myocardial expression of a constitutively active alpha 1B-adrenergic receptor in transgenic mice induces cardiac hypertrophy. 
  • N-terminal tyrosine modulation of the endocytic adaptor function of the beta-arrestins. 
  • NMR structure note: solution structure of the core domain of MESD that is essential for proper folding of LRP5/6. 
  • Neurabins recruit protein phosphatase-1 and inhibitor-2 to the actin cytoskeleton. 
  • Neutralization-guided design of HIV-1 envelope trimers with high affinity for the unmutated common ancestor of CH235 lineage CD4bs broadly neutralizing antibodies. 
  • Nitric oxide synthase-2 regulates mitochondrial Hsp60 chaperone function during bacterial peritonitis in mice. 
  • Noncatalytic domains of RGS9-1.Gbeta 5L play a decisive role in establishing its substrate specificity. 
  • Nonrandom distribution of gp120 N-linked glycosylation sites important for infectivity of human immunodeficiency virus type 1. 
  • Novel human alpha1a-adrenoceptor single nucleotide polymorphisms alter receptor pharmacology and biological function. 
  • Novel sodium channel gene mutations in Blattella germanica reduce the sensitivity of expressed channels to deltamethrin. 
  • Nuclear receptor LXRalpha is involved in cAMP-mediated human renin gene expression. 
  • Optimal self-cleavage activity of the hepatitis delta virus RNA is dependent on a homopurine base pair in the ribozyme core. 
  • Overproduction and purification of glutaryl 7-amino cephalosporanic acid acylase. 
  • POWRS: position-sensitive motif discovery. 
  • PP1 control of M phase entry exerted through 14-3-3-regulated Cdc25 dephosphorylation. 
  • Partial glycosylation at asparagine-2181 of the second C-type domain of human factor V modulates assembly of the prothrombinase complex. 
  • Pde1 phosphodiesterase modulates cyclic AMP levels through a protein kinase A-mediated negative feedback loop in Cryptococcus neoformans. 
  • Persistent tetrodotoxin-sensitive sodium current resulting from U-to-C RNA editing of an insect sodium channel. 
  • Phenotype, Genotype, and Drug Resistance in Subtype C HIV-1 Infection. 
  • Phosducin, potential role in modulation of olfactory signaling. 
  • Phosphorylation and nuclear translocation of a regulator of G protein signaling (RGS10). 
  • Phosphorylation of Ser136 is critical for potent bone sialoprotein-mediated nucleation of hydroxyapatite crystals. 
  • Phosphorylation of Ser158 regulates inflammatory redox-dependent hepatocyte nuclear factor-4alpha transcriptional activity. 
  • Phosphorylation of Threonine 794 on Tie1 by Rac1/PAK1 Reveals a Novel Angiogenesis Regulatory Pathway. 
  • Plasmids for expression of chimeric and truncated kinesin proteins. 
  • Pleckstrin homology domain-mediated membrane association and activation of the beta-adrenergic receptor kinase requires coordinate interaction with G beta gamma subunits and lipid. 
  • Post-transcriptional regulation of myotube elongation and myogenesis by Hoi Polloi. 
  • Potent inhibition of human immunodeficiency virus type 1 (HIV-1) replication by inducible expression of HIV-1 PR multimers. 
  • Pre-clinical development of a recombinant, replication-competent adenovirus serotype 4 vector vaccine expressing HIV-1 envelope 1086 clade C. 
  • Precise arrangement of factor-binding sites is required for murine CD4 promoter function. 
  • Prenylcysteine lyase deficiency in mice results in the accumulation of farnesylcysteine and geranylgeranylcysteine in brain and liver. 
  • Probing domain interactions in soluble guanylate cyclase. 
  • Probing for Binding Regions of the FtsZ Protein Surface through Site-Directed Insertions: Discovery of Fully Functional FtsZ-Fluorescent Proteins. 
  • Probing the domain structure of FtsZ by random truncation and insertion of GFP. 
  • Programmable mutually exclusive alternative splicing for generating RNA and protein diversity. 
  • Propensity for a leucine zipper-like domain of human immunodeficiency virus type 1 gp41 to form oligomers correlates with a role in virus-induced fusion rather than assembly of the glycoprotein complex. 
  • Properties of chimeric prostacyclin/prostaglandin D2 receptors: site-directed mutagenesis reveals the significance of the isoleucine residue at position 323. 
  • Properties of the Arg376 residue of the proton-coupled folate transporter (PCFT-SLC46A1) and a glutamine mutant causing hereditary folate malabsorption. 
  • Protease-activated receptor 2 (PAR2) protein and transient receptor potential vanilloid 4 (TRPV4) protein coupling is required for sustained inflammatory signaling. 
  • Protein farnesyltransferase. 
  • Protein kinase C phosphorylates nonmuscle myosin-II heavy chain from Drosophila but regulation of myosin function by this enzyme is not required for viability in flies. 
  • QM/MM model of the mouse olfactory receptor MOR244-3 validated by site-directed mutagenesis experiments. 
  • Quorum-sensing regulation of a copper toxicity system in Pseudomonas aeruginosa. 
  • R5 clade C SHIV strains with tier 1 or 2 neutralization sensitivity: tools to dissect env evolution and to develop AIDS vaccines in primate models. 
  • Rac1, a low-molecular-mass GTP-binding-protein with high intrinsic GTPase activity and distinct biochemical properties. 
  • Rapamycin antifungal action is mediated via conserved complexes with FKBP12 and TOR kinase homologs in Cryptococcus neoformans. 
  • Ras induces p21Cip1/Waf1 cyclin kinase inhibitor transcriptionally through Sp1-binding sites. 
  • Rational design and generation of recombinant control reagents for bispecific antibodies through CDR mutagenesis. 
  • Re-design of Rhodobacter sphaeroides dimethyl sulfoxide reductase. Enhancement of adenosine N1-oxide reductase activity. 
  • Recombinant Rhodobacter capsulatus xanthine dehydrogenase, a useful model system for the characterization of protein variants leading to xanthinuria I in humans. 
  • Refined solution structure of the LpxC-TU-514 complex and pKa analysis of an active site histidine: insights into the mechanism and inhibitor design. 
  • Reg1p targets protein phosphatase 1 to dephosphorylate hexokinase II in Saccharomyces cerevisiae: characterizing the effects of a phosphatase subunit on the yeast proteome. 
  • Regulation of arrestin-3 phosphorylation by casein kinase II. 
  • Regulation of intrasteric inhibition of the multifunctional calcium/calmodulin-dependent protein kinase. 
  • Regulation of the ATM-activator protein Aven by CRM1-dependent nuclear export. 
  • Regulation of the uncoupling protein-2 gene in INS-1 beta-cells by oleic acid. 
  • Regulation of zipper-interacting protein kinase activity in vitro and in vivo by multisite phosphorylation. 
  • Regulatory elements governing transcription in specialized myofiber subtypes. 
  • Reinterpretation of GCN4-p1 folding kinetics: partial helix formation precedes dimerization in coiled coil folding. 
  • Relationships between CD4 independence, neutralization sensitivity, and exposure of a CD4-induced epitope in a human immunodeficiency virus type 1 envelope protein. 
  • Requirement for Abl kinases in T cell receptor signaling. 
  • Response of blood leukocytes to thrombin receptor peptides. 
  • Responsiveness of G protein-coupled odorant receptors is partially attributed to the activation mechanism. 
  • Role for topoisomerase 1 in transcription-associated mutagenesis in yeast. 
  • Role of C-terminal serines in desensitization and phosphorylation of the mouse thromboxane receptor. 
  • Role of Cryptococcus neoformans Rho1 GTPases in the PKC1 signaling pathway in response to thermal stress. 
  • Role of GSK3 beta in behavioral abnormalities induced by serotonin deficiency. 
  • Role of arginine 180 and glutamic acid 177 of ricin toxin A chain in enzymatic inactivation of ribosomes. 
  • Role of beta-arrestins in the intracellular trafficking of G-protein-coupled receptors. 
  • Role of conserved gp41 cysteine residues in the processing of human immunodeficiency virus envelope precursor and viral infectivity. 
  • Role of conserved residues within the carboxy phosphate domain of carbamoyl phosphate synthetase. 
  • Role of conserved tyrosine 343 in intramolecular electron transfer in human sulfite oxidase. 
  • Role of phosphate-magnesium-binding regions in the high GTPase activity of rac1 protein. 
  • Role of residue 147 in the gene regulatory function of the Escherichia coli purine repressor. 
  • Role of the beta-adrenergic receptor kinase in desensitization of the alpha 2-adrenergic receptor. 
  • Role of the sixth transmembrane segment of domain IV of the cockroach sodium channel in the action of sodium channel blocker insecticides. 
  • Saturation mutagenesis of His114 of EcoRI reveals relaxed-specificity mutants. 
  • Secondary structure of the self-cleaving RNA of hepatitis delta virus: applications to catalytic RNA design. 
  • Selective mutations in cloned and expressed alpha-macroglobulin receptor binding fragment alter binding to either the alpha2-macroglobulin signaling receptor or the low density lipoprotein receptor-related protein/alpha2-macroglobulin receptor. 
  • Sensory attributes of complex tasting divalent salts are mediated by TRPM5 and TRPV1 channels. 
  • Septin phosphorylation and coiled-coil domains function in cell and septin ring morphology in the filamentous fungus Ashbya gossypii. 
  • Sequence composition and context effects on the generation and repair of frameshift intermediates in mononucleotide runs in Saccharomyces cerevisiae. 
  • Sequence variations at I260 and A1731 contribute to persistent currents in Drosophila sodium channels. 
  • Serine 232 of the alpha(2A)-adrenergic receptor is a protein kinase C-sensitive effector coupling switch. 
  • Seven-up inhibits ultraspiracle-based signaling pathways in vitro and in vivo. 
  • Site-directed mutagenesis of recombinant sulfite oxidase: identification of cysteine 207 as a ligand of molybdenum. 
  • Site-directed mutagenesis of the inhibitory subunit of retinal rod cyclic GMP phosphodiesterase. 
  • Site-directed mutagenesis studies of the high-affinity streptavidin-biotin complex: contributions of tryptophan residues 79, 108, and 120. 
  • Site-specific conjugation of a temperature-sensitive polymer to a genetically-engineered protein. 
  • Site-specific mutagenesis of Drosophila alcohol dehydrogenase: evidence for involvement of tyrosine-152 and lysine-156 in catalysis. 
  • Site-specific mutations of FtsZ--effects on GTPase and in vitro assembly. 
  • Sites of interaction between kinase-related protein and smooth muscle myosin. 
  • Sliding clamp of the bacteriophage T4 polymerase has open and closed subunit interfaces in solution. 
  • Spartin activates atrophin-1-interacting protein 4 (AIP4) E3 ubiquitin ligase and promotes ubiquitination of adipophilin on lipid droplets. 
  • Specialization of the HOG pathway and its impact on differentiation and virulence of Cryptococcus neoformans. 
  • Specific binding of RGS9-Gbeta 5L to protein anchor in photoreceptor membranes greatly enhances its catalytic activity. 
  • Stable interaction between beta-arrestin 2 and angiotensin type 1A receptor is required for beta-arrestin 2-mediated activation of extracellular signal-regulated kinases 1 and 2. 
  • Steroidogenic factor 1 is the essential transcript of the mouse Ftz-F1 gene. 
  • Streptavidin-biotin binding energetics. 
  • Structural analyses of Ca²⁺/CaM interaction with NaV channel C-termini reveal mechanisms of calcium-dependent regulation. 
  • Structural and Functional Analyses Reveal Insights into the Molecular Properties of the Escherichia coli Z Ring Stabilizing Protein, ZapC. 
  • Structural basis for μ-opioid receptor binding and activation. 
  • Structural basis of Rev1-mediated assembly of a quaternary vertebrate translesion polymerase complex consisting of Rev1, heterodimeric polymerase (Pol) ζ, and Pol κ. 
  • Structural basis of pilus subunit recognition by the PapD chaperone. 
  • Structural conservation of ion conduction pathways in K channels and glutamate receptors. 
  • Structural determinants of autoproteolysis of the Haemophilus influenzae Hap autotransporter. 
  • Structural determinants of the interaction between the Haemophilus influenzae Hap autotransporter and fibronectin. 
  • Structural requirements for association of neurofascin with ankyrin. 
  • Structural requirements regulate endoproteolytic release of the L-selectin (CD62L) adhesion receptor from the cell surface of leukocytes. 
  • Structure and function of the glycosaminoglycan binding site of chemokine macrophage-inflammatory protein-1 beta. 
  • Structure and function of the visual arrestin oligomer. 
  • Structure of the manganese-bound manganese transport regulator of Bacillus subtilis. 
  • Structure of the outer membrane translocator domain of the Haemophilus influenzae Hia trimeric autotransporter. 
  • Structure-based design of robust glucose biosensors using a Thermotoga maritima periplasmic glucose-binding protein. 
  • Structure-based redesign of corepressor specificity of the Escherichia coli purine repressor by substitution of residue 190. 
  • Structure-function relationships of the complement regulatory protein, CD59. 
  • Synaptically targeted narp plays an essential role in the aggregation of AMPA receptors at excitatory synapses in cultured spinal neurons. 
  • Synergistic interaction between two cockroach sodium channel mutations and a tobacco budworm sodium channel mutation in reducing channel sensitivity to a pyrethroid insecticide. 
  • Targeted developmental overexpression of calmodulin induces proliferative and hypertrophic growth of cardiomyocytes in transgenic mice. 
  • Targeted gene replacement demonstrates that myristoyl-CoA: protein N-myristoyltransferase is essential for viability of Cryptococcus neoformans. 
  • Targeted ubiquitination of CDT1 by the DDB1-CUL4A-ROC1 ligase in response to DNA damage. 
  • Ternary complex structure of human HGPRTase, PRPP, Mg2+, and the inhibitor HPP reveals the involvement of the flexible loop in substrate binding. 
  • Tetramerization and single-stranded DNA binding properties of native and mutated forms of murine mitochondrial single-stranded DNA-binding proteins. 
  • The A and B isoforms of the human progesterone receptor operate through distinct signaling pathways within target cells. 
  • The Chimpanzee SIV Envelope Trimer: Structure and Deployment as an HIV Vaccine Template. 
  • The G protein-coupled receptor kinase 2 is a microtubule-associated protein kinase that phosphorylates tubulin. 
  • The G185R mutation disrupts function of the iron transporter Nramp2. 
  • The Haemophilus influenzae hFbpABC Fe3+ transporter: analysis of the membrane permease and development of a gallium-based screen for mutants. 
  • The NMDA receptor M3 segment is a conserved transduction element coupling ligand binding to channel opening. 
  • The Pseudomonas syringae type III effector tyrosine phosphatase HopAO1 suppresses innate immunity in Arabidopsis thaliana. 
  • The U1 small nuclear ribonucleoprotein (snRNP) 70K protein is transported independently of U1 snRNP particles via a nuclear localization signal in the RNA-binding domain. 
  • The activation mechanism of rat vanilloid receptor 1 by capsaicin involves the pore domain and differs from the activation by either acid or heat. 
  • The autonomous activity of calcium/calmodulin-dependent protein kinase IV is required for its role in transcription. 
  • The catalytic subunit of yeast telomerase. 
  • The endothelial receptor tyrosine kinase Tie1 activates phosphatidylinositol 3-kinase and Akt to inhibit apoptosis. 
  • The estrogen receptor beta-isoform (ERbeta) of the human estrogen receptor modulates ERalpha transcriptional activity and is a key regulator of the cellular response to estrogens and antiestrogens. 
  • The extra fragment of the iron-sulfur protein (residues 96-107) of Rhodobacter sphaeroides cytochrome bc1 complex is required for protein stability. 
  • The human glutathione S-transferase P1 protein is phosphorylated and its metabolic function enhanced by the Ser/Thr protein kinases, cAMP-dependent protein kinase and protein kinase C, in glioblastoma cells. 
  • The importance of three membrane-distal tyrosines in the adaptor protein NTAL/LAB. 
  • The inducibly expressed GTPase localizes to the endoplasmic reticulum, independently of GTP binding. 
  • The integrity of a cholesterol-binding pocket in Niemann-Pick C2 protein is necessary to control lysosome cholesterol levels. 
  • The mechanism of RU486 antagonism is dependent on the conformation of the carboxy-terminal tail of the human progesterone receptor. 
  • The molecular basis for ligand specificity in a mouse olfactory receptor: a network of functionally important residues. 
  • The monomeric receptor binding domain of tetrameric α2-macroglobulin binds to cell surface GRP78 triggering equivalent activation of signaling cascades. 
  • The nucleocapsid region of HIV-1 Gag cooperates with the PTAP and LYPXnL late domains to recruit the cellular machinery necessary for viral budding. 
  • The nucleolar localization domain of the catalytic subunit of human telomerase. 
  • The products of gene I and the overlapping in-frame gene XI are required for filamentous phage assembly. 
  • The protein backbone makes important contributions to 4-oxalocrotonate tautomerase enzyme catalysis: understanding from theory and experiment. 
  • The rRNA-encoding DNA array has an altered structure in topoisomerase I mutants of Saccharomyces cerevisiae. 
  • The regulation of DNA topoisomerase II by casein kinase II. 
  • The relationship between ligand-binding thermodynamics and protein-ligand interaction forces measured by atomic force microscopy. 
  • The release of Alzheimer's disease beta amyloid peptide is reduced by phorbol treatment. 
  • The role of inactivation in open-channel block of the sodium channel: studies with inactivation-deficient mutant channels. 
  • The role of the third beta strand in gp120 conformation and neutralization sensitivity of the HIV-1 primary isolate DH012. 
  • The staphylococcal QacR multidrug regulator binds a correctly spaced operator as a pair of dimers. 
  • The structural distribution of cooperative interactions in proteins: analysis of the native state ensemble. 
  • The structural mechanism of GTP stabilized oligomerization and catalytic activation of the Toxoplasma gondii uracil phosphoribosyltransferase. 
  • The structure of PurR mutant L54M shows an alternative route to DNA kinking. 
  • The type I and type II bovine scavenger receptors expressed in Chinese hamster ovary cells are trimeric proteins with collagenous triple helical domains comprising noncovalently associated monomers and Cys83-disulfide-linked dimers. 
  • The unusual UBZ domain of Saccharomyces cerevisiae polymerase η. 
  • The vibrio cholerae hybrid sensor kinase VieS contributes to motility and biofilm regulation by altering the cyclic diguanylate level. 
  • Thermodynamic effects of reduction of the active-site disulfide of Escherichia coli thioredoxin explored by differential scanning calorimetry. 
  • Thermodynamics of metal ion binding. 2. Metal ion binding by carbonic anhydrase variants. 
  • Three amino acid substitutions in domain I of calmodulin prevent the activation of chicken smooth muscle myosin light chain kinase. 
  • Three-in-one chromatography-free purification, tag removal, and site-specific modification of recombinant fusion proteins using sortase A and elastin-like polypeptides. 
  • Thrombin-catalyzed activation of recombinant human factor V. 
  • Transcriptional regulation of the cholesteryl ester transfer protein gene by the orphan nuclear hormone receptor apolipoprotein AI regulatory protein-1. 
  • Transglutaminase 2 protects against ischemic insult, interacts with HIF1beta, and attenuates HIF1 signaling. 
  • TrkB-Shc Signaling Protects against Hippocampal Injury Following Status Epilepticus. 
  • Two amino acid residues determine 2-APB sensitivity of the ion channels TRPV3 and TRPV4. 
  • Two cyclophilin A homologs with shared and distinct functions important for growth and virulence of Cryptococcus neoformans. 
  • Two-dimensional protein crystallization via metal-ion coordination by naturally occurring surface histidines. 
  • Ultralarge complexes of PF4 and heparin are central to the pathogenesis of heparin-induced thrombocytopenia. 
  • Ultrastructure and function of dimeric, soluble intercellular adhesion molecule-1 (ICAM-1). 
  • Unexpected effects of FERM domain mutations on catalytic activity of Jak3: structural implication for Janus kinases. 
  • Use of site-directed mutagenesis to enhance the epitope-shielding effect of covalent modification of proteins with polyethylene glycol. 
  • Visualizing an olfactory sensory map. 
  • Visualizing conformation transitions of the Lipid II flippase MurJ. 
  • WT1 and DAX-1 inhibit aromatase P450 expression in human endometrial and endometriotic stromal cells. 
  • Wingless signaling generates pattern through two distinct mechanisms. 
  • X-ray crystallographic studies of streptavidin mutants binding to biotin. 
  • c-IAP1 is cleaved by caspases to produce a proapoptotic C-terminal fragment. 
  • cis element/transcription factor analysis (cis/TF): a method for discovering transcription factor/cis element relationships. 
  • epitopes immediately below the base of the V3 loop of gp120 as targets for the initial autologous neutralizing antibody response in two HIV-1 subtype B-infected individuals. 
  • p38 Mitogen-activated protein kinase mediates a negative inotropic effect in cardiac myocytes. 
  • p38-MAPK induced dephosphorylation of alpha-tropomyosin is associated with depression of myocardial sarcomeric tension and ATPase activity. 
• 

  Keywords of People

  • Bejsovec, Amy, Associate Professor of Biology, Biology
  • Bennett, Vann, George Barth Geller Distinguished Professor of Molecular Biology, Duke Cancer Institute
  • Buckley, Rebecca Hatcher, James Buren Sidbury Distinguished Professor Emeritus of Pediatrics, in the School of Medicine, Pediatrics, Allergy and Immunology
  • Erickson, Harold Paul, James B. Duke Distinguished Professor Emeritus, Cell Biology
  • Guan, Ziqiang, Research Professor in Biochemistry, Biochemistry
  • McDonnell, Donald Patrick, Glaxo-Wellcome Distinguished Professor of Molecular Cancer Biology, in the School of Medicine, Cell Biology
  • Milano, Carmelo Alessio, Joseph W. and Dorothy W. Beard Distinguished Professor of Experimental Surgery, Surgery, Cardiovascular and Thoracic Surgery
  • Pitt, Geoffrey Stuart, Adjunct Professor in the Department of Medicine, Medicine, Cardiology
  • Sampson, John Howard, Robert H., M.D. and Gloria Wilkins Professor of Neurosurgery, in the School of Medicine, Biomedical Engineering
  • Starmer, Charles Franklin, Professor Emeritus of Computer Science, Computer Science
  • Taylor, Gregory Alan, Professor in Medicine, Integrative Immunobiology