CHO Cells

• 

  Subject Areas on Research

  • 3,4-Diaryl-5-hydroxyfuranones: highly selective inhibitors of cyclooxygenase-2 with aqueous solubility. 
  • 80K-H acts as a signaling bridge in intact living cells between PKCzeta and the GLUT4 translocation regulator Munc18c. 
  • A constitutively active mutant beta 2-adrenergic receptor is constitutively desensitized and phosphorylated. 
  • A highly conserved tyrosine residue in G protein-coupled receptors is required for agonist-mediated beta 2-adrenergic receptor sequestration. 
  • A mechanism for rapacuronium-induced bronchospasm: M2 muscarinic receptor antagonism. 
  • A mutation-induced activated state of the beta 2-adrenergic receptor. Extending the ternary complex model. 
  • A new helper phospholipid for gene delivery. 
  • A new structural variation on the methanesulfonylphenyl class of selective cyclooxygenase-2 inhibitors. 
  • A role for myocilin in receptor-mediated endocytosis. 
  • A small molecule inhibitor of alpha4 integrin-dependent cell migration. 
  • ASB2α, an E3 ubiquitin ligase specificity subunit, regulates cell spreading and triggers proteasomal degradation of filamins by targeting the filamin calponin homology 1 domain. 
  • Activation of G551D CFTR channel with MPB-91: regulation by ATPase activity and phosphorylation. 
  • Addition of the BMP4 antagonist, noggin, disrupts avian inner ear development. 
  • Agonist/endogenous peptide-MHC heterodimers drive T cell activation and sensitivity. 
  • Amiloride docking to acid-sensing ion channel-1. 
  • Amphiphysin 1 binds the cyclin-dependent kinase (cdk) 5 regulatory subunit p35 and is phosphorylated by cdk5 and cdc2. 
  • Antigenicity and immunogenicity of a trimeric envelope protein from an Indian clade C HIV-1 isolate. 
  • Apoptosis is the mode of cell death caused by carcinogenic chromium. 
  • Aquaporin 6 binds calmodulin in a calcium-dependent manner. 
  • Beta-adrenergic receptor sequestration. A potential mechanism of receptor resensitization. 
  • Biochemical and pharmacological profile of a tetrasubstituted furanone as a highly selective COX-2 inhibitor. 
  • Bone morphogenetic protein signaling by hemojuvelin regulates hepcidin expression. 
  • Brain-specific carnitine palmitoyl-transferase-1c: role in CNS fatty acid metabolism, food intake, and body weight. 
  • CD19 maps to a region of conservation between human chromosome 16 and mouse chromosome 7. 
  • CD58/LFA-3 and IL-12 provided by activated monocytes are critical in the in vitro expansion of CD56+ T cells. 
  • CREB and ChREBP oppositely regulate SIRT1 expression in response to energy availability. 
  • Calcium-sensitive potassium channelopathy in human epilepsy and paroxysmal movement disorder. 
  • Cell-enhanced dissolution of carcinogenic lead chromate particles: the role of individual dissolution products in clastogenesis. 
  • Characterization of a murine monoclonal antibody that mimics heparin-induced thrombocytopenia antibodies. 
  • Characterization of differences between rapid agonist-dependent phosphorylation and phorbol ester-mediated phosphorylation of human substance P receptor in intact cells. 
  • Characterization of host-cell line specific glycosylation profiles of early transmitted/founder HIV-1 gp120 envelope proteins. 
  • Characterization of the murine BEK fibroblast growth factor (FGF) receptor: activation by three members of the FGF family and requirement for heparin. 
  • Chromium(III) picolinate produces chromosome damage in Chinese hamster ovary cells. 
  • Clastogenicity of lead chromate particles in hamster and human cells. 
  • Clinical and metabolic correction of pompe disease by enzyme therapy in acid maltase-deficient quail. 
  • Clinical isolates of measles virus use CD46 as a cellular receptor. 
  • Copper-stimulated endocytosis and degradation of the human copper transporter, hCtr1. 
  • Cowpox virus encodes a fifth member of the tumor necrosis factor receptor family: a soluble, secreted CD30 homologue. 
  • Cross-dressing the virion: the transcapsidation of adeno-associated virus serotypes functionally defines subgroups. 
  • Cutting edge: CD91-independent cross-presentation of GRP94(gp96)-associated peptides. 
  • Cyclosporin A inhibits chromium(VI)-induced apoptosis and mitochondrial cytochrome c release and restores clonogenic survival in CHO cells. 
  • Cytosolic delivery of macromolecules: I. Synthesis and characterization of pH-sensitive acyloxyalkylimidazoles. 
  • DNA damage induced by carcinogenic lead chromate particles in cultured mammalian cells. 
  • Deletion of V335 from the L2 domain of the insulin receptor results in a conformationally abnormal receptor that is unable to bind insulin and causes Donohue's syndrome in a human subject. 
  • Deletion of the glutamate carboxypeptidase II gene in mice reveals a second enzyme activity that hydrolyzes N-acetylaspartylglutamate. 
  • Dendritic cells engineered to secrete anti-GITR antibodies are effective adjuvants to dendritic cell-based immunotherapy. 
  • Design, synthesis, and analysis of antagonists of GPR55: Piperidine-substituted 1,3,4-oxadiazol-2-ones. 
  • Differential desensitization and phosphorylation of three cloned and transfected alpha 2-adrenergic receptor subtypes. 
  • Directionality of temperature activation in mouse TRPA1 ion channel can be inverted by single-point mutations in ankyrin repeat six. 
  • Distinct pathways of Gi- and Gq-mediated mitogen-activated protein kinase activation. 
  • Dopamine transporter expression confers cytotoxicity to low doses of the parkinsonism-inducing neurotoxin 1-methyl-4-phenylpyridinium. 
  • Dynamic movements of organelles containing Niemann-Pick C1 protein: NPC1 involvement in late endocytic events. 
  • Dynamics and elasticity of the fibronectin matrix in living cell culture visualized by fibronectin-green fluorescent protein. 
  • Effect of O6-benzylguanine on nitrogen mustard-induced toxicity, apoptosis, and mutagenicity in Chinese hamster ovary cells. 
  • Effects of granulocyte-macrophage colony stimulating factor produced in Chinese hamster ovary cells (regramostim), Escherichia coli (molgramostim) and yeast (sargramostim) on priming peripheral blood progenitor cells for use with autologous bone marrow after high-dose chemotherapy. 
  • Engineered pluripotent mesenchymal cells integrate and differentiate in regenerating bone: a novel cell-mediated gene therapy. 
  • Engraftment of a galactose receptor footprint onto adeno-associated viral capsids improves transduction efficiency. 
  • Enhancement of anti-tumor immunity through local modulation of CTLA-4 and GITR by dendritic cells. 
  • Epidermal growth factor receptor cooperates with signal transducer and activator of transcription 3 to induce epithelial-mesenchymal transition in cancer cells via up-regulation of TWIST gene expression. 
  • Erythropoietin and erythropoietin receptor expression in human prostate cancer. 
  • Estimation of cell survival in tumours heated to nonuniform temperature distributions. 
  • Ethnomedicinally selected plants as sources of potential analgesic compounds: indication of in vitro biological activity in receptor binding assays. 
  • Exposure to azide markedly decreases the abundance of mRNAs encoding cholesterol synthetic enzymes and inhibits cholesterol synthesis. 
  • Expression of erythropoietin receptor splice variants in human cancer. 
  • Expression of the DMT1 (NRAMP2/DCT1) iron transporter in mice with genetic iron overload disorders. 
  • Expression of the Salmonella spp. virulence factor SifA in yeast alters Rho1 activity on peroxisomes. 
  • Expression, enzyme activity, and subcellular localization of mammalian target of rapamycin in insulin-responsive cells. 
  • Functional analysis of the putative integrin recognition motif on adeno-associated virus 9. 
  • Functional expression of human 5-HT1A receptors and differential coupling to second messengers in CHO cells. 
  • G(o)-protein alpha-subunits activate mitogen-activated protein kinase via a novel protein kinase C-dependent mechanism. 
  • GPR171 is a hypothalamic G protein-coupled receptor for BigLEN, a neuropeptide involved in feeding. 
  • Generation and Characterization of a Bivalent HIV-1 Subtype C gp120 Protein Boost for Proof-of-Concept HIV Vaccine Efficacy Trials in Southern Africa. 
  • Generation and characterization of a bivalent protein boost for future clinical trials: HIV-1 subtypes CR01_AE and B gp120 antigens with a potent adjuvant. 
  • Glycan binding avidity determines the systemic fate of adeno-associated virus type 9. 
  • Glycosaminoglycan binding properties of natural venezuelan equine encephalitis virus isolates. 
  • Glycosaminoglycans modulate fibronectin matrix assembly and are essential for matrix incorporation of tenascin-C. 
  • Glycosylation Benchmark Profile for HIV-1 Envelope Glycoprotein Production Based on Eleven Env Trimers. 
  • Growth hormone regulates phosphorylation and function of CCAAT/enhancer-binding protein beta by modulating Akt and glycogen synthase kinase-3. 
  • HIN-1, a putative cytokine highly expressed in normal but not cancerous mammary epithelial cells. 
  • Heteromeric assembly of acid-sensitive ion channel and epithelial sodium channel subunits. 
  • High XRCC1 protein expression is associated with poorer survival in patients with head and neck squamous cell carcinoma. 
  • High-level production of recombinant human lysosomal acid alpha-glucosidase in Chinese hamster ovary cells which targets to heart muscle and corrects glycogen accumulation in fibroblasts from patients with Pompe disease. 
  • Human retroviral gag- and gag-pol-like proteins interact with the transforming growth factor-beta receptor activin receptor-like kinase 1. 
  • Human substance P receptor expressed in Chinese hamster ovary cells directly activates G(alpha q/11), G(alpha s), G(alpha o). 
  • Identification and biochemical studies on novel non-nucleoside inhibitors of the enzyme adenosine kinase. 
  • Identification of 80K-H as a protein involved in GLUT4 vesicle trafficking. 
  • Identification of IGFBP-6 as a significantly downregulated gene by beta-catenin in desmoid tumors. 
  • Identification of amino acid sequences in fibrinogen gamma -chain and tenascin C C-terminal domains critical for binding to integrin alpha vbeta 3. 
  • Immunochemical and molecular analysis of antigen binding to lipid anchored and soluble forms of an MHC independent human alpha/beta T cell receptor. 
  • Immunoglobulin-like domain containing receptor 1 mediates fat-stimulated cholecystokinin secretion. 
  • In vitro assessment of human nuclear hormone receptor activity and cytotoxicity of the flame retardant mixture FM 550 and its triarylphosphate and brominated components. 
  • In vitro effect of chlorpyrifos oxon on muscarinic receptors and adenylate cyclase. 
  • Increased activity of phosphatase PP2A in the presence of the PlA2 polymorphism of alphaIIbbeta3. 
  • Induction of apoptotic cell death by particulate lead chromate: differential effects of vitamins C and E on genotoxicity and survival. 
  • Induction of internucleosomal DNA fragmentation by carcinogenic chromate: relationship to DNA damage, genotoxicity, and inhibition of macromolecular synthesis. 
  • Infantile spasms and encephalopathy without preceding neonatal seizures caused by KCNQ2 R198Q, a gain-of-function variant. 
  • Inhibition of lead chromate clastogenesis by ascorbate: relationship to particle dissolution and uptake. 
  • Initiation of replication at a mammalian chromosomal origin. 
  • Insulin-triggered repositioning of munc18c on syntaxin-4 in GLUT4 signalling. 
  • Intracellular pH regulation by Na(+)/H(+) exchange requires phosphatidylinositol 4,5-bisphosphate. 
  • Investigation of interfacial properties of pure and mixed poloxamers for surfactant-mediated shear protection of mammalian cells. 
  • Knockdown of ASIC1 and epithelial sodium channel subunits inhibits glioblastoma whole cell current and cell migration. 
  • L- and P-selectins, but not CD49d (VLA-4) integrins, mediate monocyte initial attachment to TNF-alpha-activated vascular endothelium under flow in vitro. 
  • Lectin and epidermal growth factor domains of P-selectin at physiologic density are the recognition unit for leukocyte binding. 
  • Lipidomics identifies a requirement for peroxisomal function during influenza virus replication. 
  • MN and IIIB recombinant glycoprotein 120 vaccine-induced binding antibodies to native envelope glycoprotein of human immunodeficiency virus type 1 primary isolates. National Institute of Allergy and Infectious Disease Aids Vaccine Evaluation Group. 
  • Mammalian TRPV4 (VR-OAC) directs behavioral responses to osmotic and mechanical stimuli in Caenorhabditis elegans. 
  • Mast cell desensitization inhibits calcium flux and aberrantly remodels actin. 
  • Mechanisms of chromium-induced suppression of RNA synthesis in cellular and cell-free systems: relationship to RNA polymerase arrest. 
  • Mechanisms of desensitization and resensitization of G protein-coupled neurokinin1 and neurokinin2 receptors. 
  • Mimosine, a novel inhibitor of DNA replication, binds to a 50 kDa protein in Chinese hamster cells. 
  • Modeling of sequestration and down regulation in cells containing beta2-adrenergic receptors. 
  • Modulation of cyclophosphamide activity by O6-alkylguanine-DNA alkyltransferase. 
  • Modulation of ligand binding affinity of the adipocyte lipid-binding protein by selective mutation. Analysis in vitro and in situ. 
  • Multiple blocks to human immunodeficiency virus type 1 replication in rodent cells. 
  • N-(6-chloro-pyridin-3-yl)-3,4-difluoro-benzamide (ICA-27243): a novel, selective KCNQ2/Q3 potassium channel activator. 
  • N-desalkylquetiapine, a potent norepinephrine reuptake inhibitor and partial 5-HT1A agonist, as a putative mediator of quetiapine's antidepressant activity. 
  • Near-field scanning optical microscopy for bioanalysis at nanometer resolution. 
  • Negative antagonists promote an inactive conformation of the beta 2-adrenergic receptor. 
  • Nucleotide excision repair functions in the removal of chromium-induced DNA damage in mammalian cells. 
  • Oligomeric structure and tissue distribution of ficolins from mouse, pig and human. 
  • Optical coding of mammalian cells using semiconductor quantum dots. 
  • Overexpression of beta-arrestin and beta-adrenergic receptor kinase augment desensitization of beta 2-adrenergic receptors. 
  • PKA-mediated phosphorylation of the beta1-adrenergic receptor promotes Gs/Gi switching. 
  • Phorbol ester promotes endocytosis by activating a factor involved in endosome fusion. 
  • Phosphatidylinositol 3-kinase is an early intermediate in the G beta gamma-mediated mitogen-activated protein kinase signaling pathway. 
  • Phylogenetic comparisons implicate sex hormone-binding globulin in "masculinization" of the female spotted hyena (Crocuta crocuta). 
  • Phytol metabolites are circulating dietary factors that activate the nuclear receptor RXR. 
  • Platelet-derived growth factor receptor association with Na(+)/H(+) exchanger regulatory factor potentiates receptor activity. 
  • Preferential formation and repair of chromium-induced DNA adducts and DNA--protein crosslinks in nuclear matrix DNA. 
  • Protein kinase A-mediated phosphorylation of the beta 2-adrenergic receptor regulates its coupling to Gs and Gi. Demonstration in a reconstituted system. 
  • Protein kinase-zeta interacts with munc18c: role in GLUT4 trafficking. 
  • Psalmotoxin-1 docking to human acid-sensing ion channel-1. 
  • Purification, characterization, and immunogenicity of a soluble trimeric envelope protein containing a partial deletion of the V2 loop derived from SF162, an R5-tropic human immunodeficiency virus type 1 isolate. 
  • QS-21 promotes an adjuvant effect allowing for reduced antigen dose during HIV-1 envelope subunit immunization in humans. 
  • Radiolabeled guanine derivatives for the in vivo mapping of O(6)-alkylguanine-DNA alkyltransferase: 6-(4-[(18)F]Fluoro-benzyloxy)-9H-purin-2-ylamine and 6-(3-[(131)I]Iodo-benzyloxy)-9H-purin-2-ylamine. 
  • Rapid high-level production of functional HIV broadly neutralizing monoclonal antibodies in transient plant expression systems. 
  • Ras-dependent activation of fibroblast mitogen-activated protein kinase by 5-HT1A receptor via a G protein beta gamma-subunit-initiated pathway. 
  • Reciprocal in vivo regulation of myocardial G protein-coupled receptor kinase expression by beta-adrenergic receptor stimulation and blockade. 
  • Recombinant human acid alpha-glucosidase enzyme therapy for infantile glycogen storage disease type II: results of a phase I/II clinical trial. 
  • Regulation of mitogen-activated protein kinase pathways by catecholamine receptors. 
  • Release of amino-terminal fragments from amyloid precursor protein reporter and mutated derivatives in cultured cells. 
  • Role of phosphorylation in agonist-promoted beta 2-adrenergic receptor sequestration. Rescue of a sequestration-defective mutant receptor by beta ARK1. 
  • Role of platelet activating factor in the intestinal epithelial secretory and Chinese hamster ovary cell cytoskeletal responses to cholera toxin. 
  • Role of platelet-activating factor in Chinese hamster ovary cell responses to cholera toxin. 
  • SREC-I, a type F scavenger receptor, is an endocytic receptor for calreticulin. 
  • Serine 232 of the alpha(2A)-adrenergic receptor is a protein kinase C-sensitive effector coupling switch. 
  • Serotonin 5-HT1A receptor-mediated Erk activation requires calcium/calmodulin-dependent receptor endocytosis. 
  • Single-cell microarray enables high-throughput evaluation of DNA double-strand breaks and DNA repair inhibitors. 
  • Small nucleolar RNAs U32a, U33, and U35a are critical mediators of metabolic stress. 
  • Sphingosine-1-phosphate signaling regulates lamellipodia localization of cortactin complexes in endothelial cells. 
  • Stabilization of HIV-1 envelope in the CD4-bound conformation through specific cross-linking of a CD4 mimetic. 
  • Structural organization of the human insulin receptor ectodomain. 
  • Structure-function relationships of the complement regulatory protein, CD59. 
  • Structure-functional selectivity relationship studies of β-arrestin-biased dopamine D₂ receptor agonists. 
  • Sulfotransferases of two specificities function in the reconstitution of high endothelial cell ligands for L-selectin. 
  • Sulfoxide-containing aromatic nitrogen mustards as hypoxia-directed bioreductive cytotoxins. 
  • Superiority in Rhesus Macaques of Targeting HIV-1 Env gp140 to CD40 versus LOX-1 in Combination with Replication-Competent NYVAC-KC for Induction of Env-Specific Antibody and T Cell Responses. 
  • Surfactant protein D binding to Aspergillus fumigatus hyphae is calcineurin-sensitive. 
  • Synergistic regulation of beta2-adrenergic receptor sequestration: intracellular complement of beta-adrenergic receptor kinase and beta-arrestin determine kinetics of internalization. 
  • Synthesis and biological activity of a novel methylamine-bridged enkephalin analogue (MABE): a new route to cyclic peptides and peptidomimetics. 
  • Synthesis and biological evaluation of 3-heteroaryloxy-4-phenyl-2(5H)-furanones as selective COX-2 inhibitors. 
  • T cell inactivation by poxviral B22 family proteins increases viral virulence. 
  • Terminal N-linked galactose is the primary receptor for adeno-associated virus 9. 
  • The B7-2 (B70) costimulatory molecule expressed by monocytes and activated B lymphocytes is the CD86 differentiation antigen. 
  • The G protein-coupled receptor GPR4 suppresses ERK activation in a ligand-independent manner. 
  • The adaptor protein beta-arrestin2 enhances endocytosis of the low density lipoprotein receptor. 
  • The beta2-adrenergic receptor interacts with the Na+/H+-exchanger regulatory factor to control Na+/H+ exchange. 
  • The discovery of rofecoxib, [MK 966, Vioxx, 4-(4'-methylsulfonylphenyl)-3-phenyl-2(5H)-furanone], an orally active cyclooxygenase-2-inhibitor. 
  • The disulfide bonding pattern in ficolin multimers. 
  • The farnesyl protein transferase inhibitor SCH66336 is a potent inhibitor of MDR1 product P-glycoprotein. 
  • The human cytokine I-309 is a monocyte chemoattractant. 
  • The integrity of a cholesterol-binding pocket in Niemann-Pick C2 protein is necessary to control lysosome cholesterol levels. 
  • The mast cell tumor necrosis factor alpha response to FimH-expressing Escherichia coli is mediated by the glycosylphosphatidylinositol-anchored molecule CD48. 
  • The mode of death of pig kidney cells infected with cowpox virus is governed by the expression of the crmA gene. 
  • The origin of O6-methylguanine-DNA methyltransferase in Chinese hamster ovary cells transfected with human DNA. 
  • The plant amino acid mimosine may inhibit initiation at origins of replication in Chinese hamster cells. 
  • Transcriptional inhibition by carcinogenic chromate: relationship to DNA damage. 
  • Transglutaminase 2 protects against ischemic insult, interacts with HIF1beta, and attenuates HIF1 signaling. 
  • Transient receptor potential ankyrin 1 antagonists block the noxious effects of toxic industrial isocyanates and tear gases. 
  • Triphenyl phosphate-induced developmental toxicity in zebrafish: potential role of the retinoic acid receptor. 
  • Ultrastructure and function of dimeric, soluble intercellular adhesion molecule-1 (ICAM-1). 
  • Unusual chromosome architecture and behaviour at an HSR. 
  • Vanilloid receptor-related osmotically activated channel (VR-OAC), a candidate vertebrate osmoreceptor. 
  • Visualization of P-selectin glycoprotein ligand-1 as a highly extended molecule and mapping of protein epitopes for monoclonal antibodies. 
  • alphaSU2, an epithelial integrin that binds laminin in the sea urchin embryo. 
  • beta-Arrestin-dependent constitutive internalization of the human chemokine decoy receptor D6. 
  • cis-Methyldioxolane specifically recognizes the m2 muscarinic receptor. 
  • tPA-mediated generation of plasmin is catalyzed by the proteoglycan NG2. 
  • β-Arrestin-2 desensitizes the transient receptor potential vanilloid 1 (TRPV1) channel. 
• 

  Keywords of People

  • Case, Laura Elizabeth, Assistant Professor of Orthopaedic Surgery, Orthopaedics, Physical Therapy
  • Erickson, Harold Paul, James B. Duke Professor of Cell Biology, Biomedical Engineering
  • Krangel, Michael S., Mary Bernheim Professor of Immunology, Immunology
  • Muir, Kelly Walton, Associate Professor of Ophthalmology, Ophthalmology, Glaucoma
  • Sampson, John Howard, Robert H., M.D. and Gloria Wilkins Professor of Neurosurgery, in the School of Medicine, Immunology