3T3 Cells

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  Subject Areas on Research

  • A 30-kDa alternative translation product of the CCAAT/enhancer binding protein alpha message: transcriptional activator lacking antimitotic activity. 
  • A bioactive titanium foam scaffold for bone repair. 
  • A calcineurin-dependent transcriptional pathway controls skeletal muscle fiber type. 
  • A new strategy for studying protein kinase B and its three isoforms. Role of protein kinase B in phosphorylating glycogen synthase kinase-3, tuberin, WNK1, and ATP citrate lyase. 
  • A novel flow-perfusion bioreactor supports 3D dynamic cell culture. 
  • A uniform human Wnt expression library reveals a shared secretory pathway and unique signaling activities. 
  • Activated c-Abl is degraded by the ubiquitin-dependent proteasome pathway. 
  • Activation of fibroblast growth factor (FGF) receptors by recombinant human FGF-5. 
  • Activation of mitogen-activated protein kinase kinase by v-Raf in NIH 3T3 cells and in vitro. 
  • Agonist-dependent recruitment of phosphoinositide 3-kinase to the membrane by beta-adrenergic receptor kinase 1. A role in receptor sequestration. 
  • Altered distribution of mitochondria and actin fibers in 3T3 cells cultured on microcarriers. 
  • Aluminum-induced DNA synthesis in osteoblasts: mediation by a G-protein coupled cation sensing mechanism. 
  • An intron facilitates activation of the calspermin gene by the testis-specific transcription factor CREM tau. 
  • Anchorage-independent cell growth signature identifies tumors with metastatic potential. 
  • Angiotensin II stimulates canonical TGF-β signaling pathway through angiotensin type 1 receptor to induce granulation tissue contraction. 
  • Angiotensin II type 2 receptor mediates programmed cell death. 
  • Anti-mouse mesangial cell serum induces acute glomerulonephropathy in mice. 
  • Antisense inhibition of P-glycoprotein expression using peptide-oligonucleotide conjugates. 
  • Apoptosis rate can be accelerated or decelerated by overexpression or reduction of the level of elongation factor-1 alpha. 
  • Assignment of the agent of Tyzzer's disease to Clostridium piliforme comb. nov. on the basis of 16S rRNA sequence analysis. 
  • Association of protein kinase C(lambda) with adducin in 3T3-L1 adipocytes. 
  • Association of the kinesin-binding domain of RanBP2 to KIF5B and KIF5C determines mitochondria localization and function. 
  • Ataxia telangiectasia mutant protein activates c-Abl tyrosine kinase in response to ionizing radiation. 
  • Autocrine stimulation of mas oncogene leads to altered growth control. 
  • BCL2 interaction with actin in vitro may inhibit cell motility by enhancing actin polymerization. 
  • Basic fibroblast growth factor downregulates Bcl-2 and promotes apoptosis in MCF-7 human breast cancer cells. 
  • Binding of tenascin-C to soluble fibronectin and matrix fibrils. 
  • Bioactive surface modification of metal oxides via catechol-bearing modular peptides: multivalent-binding, surface retention, and peptide bioactivity. 
  • Brain tumors in mice are susceptible to blockade of epidermal growth factor receptor (EGFR) with the oral, specific, EGFR-tyrosine kinase inhibitor ZD1839 (iressa). 
  • CCAAT/enhancer-binding protein alpha is required for transcription of the beta 3-adrenergic receptor gene during adipogenesis. 
  • CFTR as a cAMP-dependent regulator of sodium channels. 
  • Calspermin gene transcription is regulated by two cyclic AMP response elements contained in an alternative promoter in the calmodulin kinase IV gene. 
  • Cells en route to apoptosis are characterized by the upregulation of c-fos, c-myc, c-jun, cdc2, and RB phosphorylation, resembling events of early cell-cycle traverse. 
  • Cellular and viral splicing factors can modify the splicing pattern of CFTR transcripts carrying splicing mutations. 
  • Characterization of PDK2 activity against protein kinase B gamma. 
  • Characterization of senescence- and apoptosis-dependent forms of terminin as derived from a precursor found in replicating and nonreplicating cells. 
  • Cloning, heterologous expression, and chromosomal localization of human inositol polyphosphate 1-phosphatase. 
  • Combined bone morphogenetic protein-2 and -7 gene transfer enhances osteoblastic differentiation and spine fusion in a rodent model. 
  • Commitment to cell death is signaled by the appearance of a terminin protein of 30 kDa. 
  • Concomitant control of mechanical properties and degradation in resorbable elastomer-like materials using stereochemistry and stoichiometry for soft tissue engineering. 
  • Context-specific effects of fibulin-5 (DANCE/EVEC) on cell proliferation, motility, and invasion. Fibulin-5 is induced by transforming growth factor-beta and affects protein kinase cascades. 
  • Control of hepatic gluconeogenesis through the transcriptional coactivator PGC-1. 
  • Cooperation between core binding factor and adjacent promoter elements contributes to the tissue-specific expression of interleukin-3. 
  • Correction of aberrant splicing of the cystic fibrosis transmembrane conductance regulator (CFTR) gene by antisense oligonucleotides. 
  • Correction of proliferative responses in purine nucleoside phosphorylase (PNP)-deficient T lymphocytes by retroviral-mediated PNP gene transfer and expression. 
  • Cyclin D1 inhibits peroxisome proliferator-activated receptor gamma-mediated adipogenesis through histone deacetylase recruitment. 
  • Cytokine induction of nuclear factor kappa B in cycling and growth-arrested cells. Evidence for cell cycle-independent activation. 
  • Dancing with different partners: protein kinase a phosphorylation of seven membrane-spanning receptors regulates their G protein-coupling specificity. 
  • Deletion of the carboxyl terminus of Tie2 enhances kinase activity, signaling, and function. Evidence for an autoinhibitory mechanism. 
  • Deltex1 redirects lymphoid progenitors to the B cell lineage by antagonizing Notch1. 
  • Derivation of a biologically contained replication system for human immunodeficiency virus type 1. 
  • Design of a novel small peptide targeted against a tumor-specific receptor. 
  • Development of an ER action indicator mouse for the study of estrogens, selective ER modulators (SERMs), and Xenobiotics. 
  • Dietary flavonoid naringenin induces regulatory T cells via an aryl hydrocarbon receptor mediated pathway. 
  • Differential effects of insulin-like growth factor (IGF)-binding protein-3 and its proteolytic fragments on ligand binding, cell surface association, and IGF-I receptor signaling. 
  • Direct-contact co-culture between smooth muscle and endothelial cells inhibits TNF-alpha-mediated endothelial cell activation. 
  • Do Surface Porosity and Pore Size Influence Mechanical Properties and Cellular Response to PEEK? 
  • Dominant-negative mutants of Grb2 induced reversal of the transformed phenotypes caused by the point mutation-activated rat HER-2/Neu. 
  • Down-regulation of statin, a nonproliferation-specific nuclear protein, and up-regulation of c-myc after initiation of programmed cell death in mouse fibroblasts. 
  • Dual labeling of the fibronectin matrix and actin cytoskeleton with green fluorescent protein variants. 
  • EJ-Ras inhibits phospholipase C gamma 1 but not actin polymerization induced by platelet-derived growth factor-BB via phosphatidylinositol 3-kinase. 
  • ELAV protein HuA (HuR) can redistribute between nucleus and cytoplasm and is upregulated during serum stimulation and T cell activation. 
  • Ectopic expression of Hel-N1, an RNA-binding protein, increases glucose transporter (GLUT1) expression in 3T3-L1 adipocytes. 
  • Effect of IGFBP-3 on IGF- and IGF-analogue-induced insulin-like growth factor-I receptor (IGFIR) signalling. 
  • Epidermal growth factor ligand-independent, unregulated, cell-transforming potential of a naturally occurring human mutant EGFRvIII gene. 
  • Eps15 is constitutively oligomerized due to homophilic interaction of its coiled-coil region. 
  • Erythrocyte plasma membrane-bound ERK1/2 activation promotes ICAM-4-mediated sickle red cell adhesion to endothelium. 
  • Evidence for a role of Met-HGF/SF during Ras-mediated tumorigenesis/metastasis. 
  • Expression of a peptide inhibitor of protein phosphatase 1 increases phosphorylation and activity of CREB in NIH 3T3 fibroblasts. 
  • Expression, enzyme activity, and subcellular localization of mammalian target of rapamycin in insulin-responsive cells. 
  • Flavanones structure-related inhibition on TPA-induced tumor promotion through suppression of extracellular signal-regulated protein kinases: involvement of prostaglandin E2 in anti-promotive process. 
  • Force fluctuations in three-dimensional suspended fibroblasts. 
  • Formulations which increase the size of lipoplexes prevent serum-associated inhibition of transfection. 
  • Foxp1 Is Indispensable for Ductal Morphogenesis and Controls the Exit of Mammary Stem Cells from Quiescence. 
  • Fusion of fibroblast growth factor 21 to a thermally responsive biopolymer forms an injectable depot with sustained anti-diabetic action. 
  • GFRα1 released by nerves enhances cancer cell perineural invasion through GDNF-RET signaling. 
  • GRP94 (gp96) and GRP94 N-terminal geldanamycin binding domain elicit tissue nonrestricted tumor suppression. 
  • Gene therapy in tissue-engineered blood vessels. 
  • Glucose activates mitogen-activated protein kinase (extracellular signal-regulated kinase) through proline-rich tyrosine kinase-2 and the Glut1 glucose transporter. 
  • Growth hormone regulates phosphorylation and function of CCAAT/enhancer-binding protein beta by modulating Akt and glycogen synthase kinase-3. 
  • HDAC6 controls major cell response pathways to cytotoxic accumulation of protein aggregates. 
  • HDAC6 is a microtubule-associated deacetylase. 
  • HEPSIN inhibits cell growth/invasion in prostate cancer cells. 
  • HER2 overexpression elicits a proinflammatory IL-6 autocrine signaling loop that is critical for tumorigenesis. 
  • HMG-CoA reductase inhibitors (statins) characterized as direct inhibitors of P-glycoprotein. 
  • Hel-N1, an RNA-binding protein, is a ligand for an A + U rich region of the GLUT1 3' UTR. 
  • High affinity for farnesyltransferase and alternative prenylation contribute individually to K-Ras4B resistance to farnesyltransferase inhibitors. 
  • Hydrophobic residues Phe751 and Leu753 are essential for STAT5 transcriptional activity. 
  • IFN-gamma production in response to neuropathogenic Cas-Br-M murine leukemia virus infection. 
  • Identification of novel Runx2 targets in osteoblasts: cell type-specific BMP-dependent regulation of Tram2. 
  • Identification of resting cells by dual-parameter flow cytometry of statin expression and DNA content. 
  • Identification of the TBX5 transactivating domain and the nuclear localization signal. 
  • Immunity-related GTPase M (IRGM) proteins influence the localization of guanylate-binding protein 2 (GBP2) by modulating macroautophagy. 
  • Immunogenic determinants of a neuropathogenic murine leukemia virus. 
  • Immunotoxins that target an oncogenic mutant epidermal growth factor receptor expressed in human tumors. 
  • Impact of polymer-bound iodine on fibronectin adsorption and osteoblast cell morphology in radiopaque medical polymers: tyrosine-derived polycarbonate blends as a model system. 
  • Improved targeting of an anti-epidermal growth factor receptor variant III monoclonal antibody in tumor xenografts after labeling using N-succinimidyl 5-iodo-3-pyridinecarboxylate. 
  • Induction of cytotoxic T cell responses in newborn mice by DNA immunization. 
  • Inhibition of 1,25-dihydroxyvitamin D3-dependent transcription by synthetic LXXLL peptide antagonists that target the activation domains of the vitamin D and retinoid X receptors. 
  • Inhibitors of prostaglandin synthesis inhibit human prostate tumor cell invasiveness and reduce the release of matrix metalloproteinases. 
  • Insulin-triggered repositioning of munc18c on syntaxin-4 in GLUT4 signalling. 
  • Interaction and functional collaboration of p300/CBP and bHLH proteins in muscle and B-cell differentiation. 
  • Interferon regulatory factor-1 up-regulates angiotensin II type 2 receptor and induces apoptosis. 
  • Lefty proteins exhibit unique processing and activate the MAPK pathway. 
  • Low human and murine Mcl-1 expression leads to a pro-apoptotic plaque phenotype enriched in giant-cells. 
  • MAP kinase signaling specificity mediated by the LIN-1 Ets/LIN-31 WH transcription factor complex during C. elegans vulval induction. 
  • MDM2-HDAC1-mediated deacetylation of p53 is required for its degradation. 
  • Macrophage embedded fibrin gels: an in vitro platform for assessing inflammation effects on implantable glucose sensors. 
  • Magnesium Catalyzed Polymerization of End Functionalized Poly(propylene maleate) and Poly(propylene fumarate) for 3D Printing of Bioactive Scaffolds. 
  • Mas oncogene receptor coupling and peptide specificity in Balb 3T3 and vascular smooth muscle cells. 
  • Membrane association of the myristoylated alanine-rich C kinase substrate (MARCKS) protein appears to involve myristate-dependent binding in the absence of a myristoyl protein receptor. 
  • Met and hepatocyte growth factor/scatter factor expression in human gliomas. 
  • Mitogens stimulate the rapid nuclear to cytosolic translocation of tristetraprolin, a potential zinc-finger transcription factor. 
  • Molecular and cellular mechanism of angiotensin II-mediated apoptosis. 
  • Multiple autocrine growth factors modulate vascular smooth muscle cell growth response to angiotensin II. 
  • Murine monoclonal antibodies specific for conserved and non-conserved antigenic determinants of the human and murine Ku autoantigens. 
  • Mutagenic analysis of functional domains of the mos proto-oncogene and identification of the sites important for MAPK activation and DNA binding. 
  • Mutations of the BRAF gene in human cancer. 
  • Myocardial injury-induced fibroblast proliferation facilitates retroviral-mediated gene transfer to the rat heart in vivo. 
  • Non-canonical Ret signaling augments p75-mediated cell death in developing sympathetic neurons. 
  • Nucleotide sequence, expression, and chromosomal mapping of Mrp and mapping of five related sequences. 
  • Oncogenic activation of c-ABL by mutation within its last exon. 
  • Optimized lymphocyte protein extraction performed simultaneously with DNA and RNA isolation: application to the study of factors affecting DNA, RNA, and protein recovery from lymphocytes of the oldest individuals. 
  • Overexpression of protein targeting to glycogen (PTG) in rat hepatocytes causes profound activation of glycogen synthesis independent of normal hormone- and substrate-mediated regulatory mechanisms. 
  • P311 induces a TGF-beta1-independent, nonfibrogenic myofibroblast phenotype. 
  • PHAS-I as a link between mitogen-activated protein kinase and translation initiation. 
  • Palmitoylation of the immunity related GTPase, Irgm1: impact on membrane localization and ability to promote mitochondrial fission. 
  • Participation of ATM in insulin signalling through phosphorylation of eIF-4E-binding protein 1. 
  • Phosphatidylinositol 4,5-bisphosphate functions as a second messenger that regulates cytoskeleton-plasma membrane adhesion. 
  • Phosphorylation of tristetraprolin, a potential zinc finger transcription factor, by mitogen stimulation in intact cells and by mitogen-activated protein kinase in vitro. 
  • Physical and biological properties of cationic triesters of phosphatidylcholine. 
  • Polyimides as biomaterials: preliminary biocompatibility testing. 
  • Protection against Mycobacterium avium by DNA vaccines expressing mycobacterial antigens as fusion proteins with green fluorescent protein. 
  • Protein phosphatase 2A dephosphorylation of phosphoserine 112 plays the gatekeeper role for BAD-mediated apoptosis. 
  • Quantitative distinctions of active site molecular recognition by P-glycoprotein and cytochrome P450 3A4. 
  • Radioiodination of internalizing monoclonal antibodies using N-succinimidyl 5-iodo-3-pyridinecarboxylate. 
  • Ras induces p21Cip1/Waf1 cyclin kinase inhibitor transcriptionally through Sp1-binding sites. 
  • Regulation of casein kinase I epsilon and casein kinase I delta by an in vivo futile phosphorylation cycle. 
  • Regulation of early adipose commitment by Zfp521. 
  • Regulation of male germ cell cycle arrest and differentiation by DND1 is modulated by genetic background. 
  • Relationship between 3T3 cell spreading and the strength of adhesion on glass and silane surfaces. 
  • Role of the asialoglycoprotein receptor in binding and entry of hepatitis C virus structural proteins in cultured human hepatocytes. 
  • Scar/WAVE-1, a Wiskott-Aldrich syndrome protein, assembles an actin-associated multi-kinase scaffold. 
  • Senescent human fibroblasts resist programmed cell death, and failure to suppress bcl2 is involved. 
  • Sequences within the coding regions of clotting factor VIII and CFTR block transcriptional elongation. 
  • Slow-release nanoparticle-encapsulated delivery system for laryngeal injection. 
  • Specific blockade of basic fibroblast growth factor gene expression in endothelial cells by antisense oligonucleotide. 
  • Statin, a protein specifically present in nonproliferating cells, is a phosphoprotein and forms a complex with a 45-kilodalton serine/threonine kinase. 
  • Stress-induced secretion of growth inhibitors: a novel tumor suppressor function of p53. 
  • Structure and regulation of the mouse ing1 gene. Three alternative transcripts encode two phd finger proteins that have opposite effects on p53 function. 
  • Suppression of antiproliferative effects of tumor necrosis factor by transfection of cells with human platelet-derived growth factor B/c-sis gene. 
  • Suppression of beta cell energy metabolism and insulin release by PGC-1alpha. 
  • Surface- and Hydrogel-Mediated Delivery of Nucleic Acid Nanoparticles. 
  • Surface-tethered DNA complexes for enhanced gene delivery 
  • Syndecans, cell surface heparan sulfate proteoglycans, are induced by a proline-rich antimicrobial peptide from wounds. 
  • TGF-beta related genes in development. 
  • Tagging ribozyme reaction sites to follow trans-splicing in mammalian cells. 
  • Targeting the Tie2/Tek receptor in astrocytomas. 
  • Tethering ribozymes to a retroviral packaging signal for destruction of viral RNA. 
  • The Ras-related protein AGS1/RASD1 suppresses cell growth. 
  • The activity of guanine exchange factor NET1 is essential for transforming growth factor-beta-mediated stress fiber formation. 
  • The beta3-adrenergic receptor activates mitogen-activated protein kinase in adipocytes through a Gi-dependent mechanism. 
  • The betagamma subunit of heterotrimeric G proteins interacts with RACK1 and two other WD repeat proteins. 
  • The death-inducing activity of RIPK1 is regulated by the pH environment. 
  • The endothelial receptor tyrosine kinase Tie1 activates phosphatidylinositol 3-kinase and Akt to inhibit apoptosis. 
  • The farnesyl protein transferase inhibitor SCH66336 is a potent inhibitor of MDR1 product P-glycoprotein. 
  • The growth-dependent expression of angiotensin II type 2 receptor is regulated by transcription factors interferon regulatory factor-1 and -2. 
  • The proto-oncogene bcl-3 encodes an I kappa B protein. 
  • Three-dimensional traction force microscopy: a new tool for quantifying cell-matrix interactions. 
  • Tissue-specific expression of Ran isoforms in the mouse. 
  • Transcriptional regulation of the cholesteryl ester transfer protein gene by the orphan nuclear hormone receptor apolipoprotein AI regulatory protein-1. 
  • Transcriptional regulation of the mouse presenilin-1 gene. 
  • Transfection of cells with transforming growth factor-alpha leads to cellular resistance to the antiproliferative effects of tumor necrosis factor. 
  • Trimers of the fibronectin cell adhesion domain localize to actin filament bundles and undergo rearward translocation. 
  • Tumour maintenance is mediated by eNOS. 
  • Tyrosine 1101 of Tie2 is the major site of association of p85 and is required for activation of phosphatidylinositol 3-kinase and Akt. 
  • Yantar, a conserved arginine-rich protein is involved in Drosophila hemocyte development. 
  • [Inhibitation of tumor angiogenesis, growth and metastasis by blocking VEGF paracrine pathway]. 
  • beta -Arrestin-mediated recruitment of the Src family kinase Yes mediates endothelin-1-stimulated glucose transport. 
  • c-Abl is activated by growth factors and Src family kinases and has a role in the cellular response to PDGF. 
  • cCXCR1 is a receptor for cIL-8 (9E3/cCAF) and its N- and C-terminal peptides and is also activated by hIL-8 (CXCL8). 
  • pp60v-src kinase overexpression leads to cellular resistance to the antiproliferative effects of tumor necrosis factor. 
  • xCT (SLC7A11)-mediated metabolic reprogramming promotes non-small cell lung cancer progression. 
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  Keywords of People

  • Bennett, Vann, George Barth Geller Distinguished Professor of Molecular Biology, Duke Cancer Institute
  • Hogan, Brigid L. M., Research Professor of Cell Biology, Cell Biology
  • McDonnell, Donald Patrick, Glaxo-Wellcome Distinguished Professor of Molecular Cancer Biology, in the School of Medicine, Cell Biology
  • McLendon, Roger Edwin, Professor of Pathology, Pathology
  • Sampson, John Howard, Robert H., M.D. and Gloria Wilkins Professor of Neurosurgery, in the School of Medicine, Biomedical Engineering
  • Taylor, Gregory Alan, Professor in Medicine, Integrative Immunobiology