Gene Transfer Techniques

• 

  Subject Areas on Research

  • A Phase I clinical trial of immunotherapy with interferon-gamma gene-modified autologous melanoma cells: monitoring the humoral immune response. 
  • A VEGF trap inhibits the beneficial effect of bFGF on vasoreactivity in corporal tissues of hypercholesterolemic rabbits. 
  • A fluorogenic C. neoformans reporter strain with a robust expression of m-cherry expressed from a safe haven site in the genome. 
  • A new helper phospholipid for gene delivery. 
  • A normothermic ex vivo organ perfusion delivery method for cardiac transplantation gene therapy. 
  • A novel approach to xenotransplantation combining surface engineering and genetic modification of isolated adult porcine islets. 
  • A novel immunocompetent murine tumor model for the evaluation of RCAd-enhanced RDAd transduction efficacy. 
  • A novel method for viral gene delivery in solid tumors. 
  • A pressure-mediated nonviral method for efficient arterial gene and oligonucleotide transfer. 
  • A requirement for NF-kappaB activation in Bcr-Abl-mediated transformation. 
  • A requirement for the rac1 GTPase in the signal transduction pathway leading to cardiac myocyte hypertrophy. 
  • A tyrosine hydroxylase-yellow fluorescent protein knock-in reporter system labeling dopaminergic neurons reveals potential regulatory role for the first intron of the rodent tyrosine hydroxylase gene. 
  • AAV9 Edits Muscle Stem Cells in Normal and Dystrophic Adult Mice. 
  • Acute host-mediated endothelial injury after adenoviral gene transfer in normal rabbit arteries: impact on transgene expression and endothelial function. 
  • Adeno-Associated Viral Vector (Serotype 2)-Nerve Growth Factor for Patients With Alzheimer Disease: A Randomized Clinical Trial. 
  • Adeno-associated viral vectors: background and technical aspects. 
  • Adenoviral gene transfer of nitric oxide synthase: high level expression in human vascular cells. 
  • Adenoviral gene transfer to the heart during cardiopulmonary bypass: effect of myocardial protection technique on transgene expression. 
  • Adenoviral-mediated gene transfer induces sustained pericardial VEGF expression in dogs: effect on myocardial angiogenesis. 
  • Adenovirus-mediated antisense urokinase-type plasminogen activator receptor gene transfer reduces tumor cell invasion and metastasis in non-small cell lung cancer cell lines. 
  • Adenovirus-mediated delivery into myocytes of muscle glycogen phosphorylase, the enzyme deficient in patients with glycogen-storage disease type V. 
  • Adenovirus-mediated gene transfer into rat cardiac allografts. Comparison of direct injection and perfusion. 
  • Adenovirus-mediated gene transfer of the beta2-adrenergic receptor to donor hearts enhances cardiac function. 
  • Adenovirus-mediated leptin expression normalises hypertension associated with diet-induced obesity. 
  • Adenovirus-mediated overexpression of uncoupling protein-2 in pancreatic islets of Zucker diabetic rats increases oxidative activity and improves beta-cell function. 
  • An engineered vascular endothelial growth factor-activating transcription factor induces therapeutic angiogenesis in ApoE knockout mice with hindlimb ischemia. 
  • An in vitro system for efficiently evaluating gene therapy approaches to hemoglobinopathies. 
  • Analysis of neuronal proliferation, migration and differentiation in the postnatal brain using equine infectious anemia virus-based lentiviral vectors. 
  • Angiotensin II type 2 receptor mediates vascular smooth muscle cell apoptosis and antagonizes angiotensin II type 1 receptor action: an in vitro gene transfer study. 
  • Autocrine and paracrine effects of atrial natriuretic peptide gene transfer on vascular smooth muscle and endothelial cellular growth. 
  • Biomaterial interactions with the immune system. 
  • Biomaterial-mediated retroviral gene transfer using self-assembled monolayers. 
  • Broadly Neutralizing Human Immunodeficiency Virus Type 1 Antibody Gene Transfer Protects Nonhuman Primates from Mucosal Simian-Human Immunodeficiency Virus Infection. 
  • Cardiac gene delivery with cardiopulmonary bypass. 
  • Cardiac muscle cell hypertrophy and apoptosis induced by distinct members of the p38 mitogen-activated protein kinase family. 
  • Catheter-mediated subselective intracoronary gene delivery to the rabbit heart: introduction of a novel method. 
  • Cell type-specific delivery of siRNAs with aptamer-siRNA chimeras. 
  • Cellular and biological therapies of gastrointestinal tumors: overview of clinical trials. 
  • Cellular and molecular mechanisms of coronary artery restenosis. 
  • Cellular cytoskeleton dynamics modulates non-viral gene delivery through RhoGTPases 
  • Cellular engineering and gene therapy strategies for insulin replacement in diabetes. 
  • Chronic suppression of heart-failure progression by a pseudophosphorylated mutant of phospholamban via in vivo cardiac rAAV gene delivery. 
  • Clara cell secretory protein decreases lung inflammation after acute virus infection. 
  • Combined bone morphogenetic protein-2 and -7 gene transfer enhances osteoblastic differentiation and spine fusion in a rodent model. 
  • Combining topographical and genetic cues to promote neuronal fate specification in stem cells. 
  • Concentrated RD114-pseudotyped MFGS-gp91phox vector achieves high levels of functional correction of the chronic granulomatous disease oxidase defect in NOD/SCID/beta -microglobulin-/- repopulating mobilized human peripheral blood CD34+ cells. 
  • Consent forms and the therapeutic misconception: the example of gene transfer research. 
  • Constitutive activation of the aromatic hydrocarbon receptor. 
  • Correction of glycogen storage disease type II by an adeno-associated virus vector containing a muscle-specific promoter. 
  • Coupling endoplasmic reticulum stress to cell death program in isolated human pancreatic islets: effects of gene transfer of Bcl-2. 
  • Cre-dependent adeno-associated virus preparation and delivery for labeling neurons in the mouse brain. 
  • Current Progress in Electrotransfection as a Nonviral Method for Gene Delivery. 
  • DNA and RNA modified dendritic cell vaccines. 
  • DNA delivery from hyaluronic acid-collagen hydrogels via a substrate-mediated approach 
  • DNA delivery from matrix metalloproteinase degradable poly (ethylene glycol) hydrogels to mouse cloned mesenchymal stem cells 
  • DNA transfer into vascular smooth muscle using fusigenic Sendai virus (HVJ)-liposomes. 
  • Delivery of phosphorodiamidate morpholino antisense oligomers in cancer cells. 
  • Department of Health and Human Services National Institutes of Health Recombinant DNA Advisory Committee. Minutes of meeting March 8-10, 2000. 
  • Design and characterization of microporous hyaluronic acid hydrogels for in vitro gene transfer to mMSCs 
  • Design of a phase 2b trial of intracoronary administration of AAV1/SERCA2a in patients with advanced heart failure: the CUPID 2 trial (calcium up-regulation by percutaneous administration of gene therapy in cardiac disease phase 2b). 
  • Development of Patient-specific AAV Vectors After Neutralizing Antibody Selection for Enhanced Muscle Gene Transfer. 
  • Differential metabolic effects of adenovirus-mediated glucokinase and hexokinase I overexpression in rat primary hepatocytes. 
  • Disappearance of body fat in normal rats induced by adenovirus-mediated leptin gene therapy. 
  • Distinct effects of endosomal escape and inhibition of endosomal trafficking on gene delivery via electrotransfection. 
  • Dorsalizing and neuralizing properties of Xdsh, a maternally expressed Xenopus homolog of dishevelled. 
  • Dose dependent effects of cardiac beta2 adrenoceptor gene therapy. 
  • Double genetic modification of adenovirus fiber with RGD polylysine motifs significantly enhances gene transfer to isolated human pancreatic islets. 
  • EJ-Ras inhibits phospholipase C gamma 1 but not actin polymerization induced by platelet-derived growth factor-BB via phosphatidylinositol 3-kinase. 
  • Effect of adenoviral-mediated transfer of transforming growth factor-beta1 on colonic anastomotic healing. 
  • Effects of pulse strength and pulse duration on in vitro DNA electromobility. 
  • Effects of rate, volume, and dose of intratumoral infusion on virus dissemination in local gene delivery. 
  • Efficient adenoviral gene transfer to early venous bypass grafts: comparison with native vessels. 
  • Efficient gene delivery to pancreatic islets with ultrasonic microbubble destruction technology. 
  • Efficient gene transfer with adeno-associated virus-based plasmids complexed to cationic liposomes for gene therapy of human prostate cancer. 
  • Efficient in vivo gene transfer into the heart in the rat myocardial infarction model using the HVJ (Hemagglutinating Virus of Japan)--liposome method. 
  • Electromobility of plasmid DNA in tumor tissues during electric field-mediated gene delivery. 
  • Embryonic and neonatal cardiac gene transfer in vivo. 
  • Emerging nanotechnology approaches for HIV/AIDS treatment and prevention. 
  • Endothelium-targeted gene and cell-based therapies for cardiovascular disease. 
  • Engineering clustered ligand binding into nonviral vectors: αvβ3 targeting as an example 
  • Engineering liver-detargeted AAV9 vectors for cardiac and musculoskeletal gene transfer. 
  • Enhanced myocardial function in transgenic mice overexpressing the beta 2-adrenergic receptor. 
  • Enhanced vasorelaxation by overexpression of beta 2-adrenergic receptors in large arteries. 
  • Enhancement of electric field-mediated gene delivery through pretreatment of tumors with a hyperosmotic mannitol solution. 
  • Enhancing stem cell therapy through genetic modification. 
  • Ependymal/subependymal zone cells of postnatal and adult songbird brain generate both neurons and nonneuronal siblings in vitro and in vivo. 
  • Erythroid-specific expression of human CD59 and transfer to vascular endothelial cells. 
  • Evasion of immune responses to introduced human acid alpha-glucosidase by liver-restricted expression in glycogen storage disease type II. 
  • Evidence for direct local effect of angiotensin in vascular hypertrophy. In vivo gene transfer of angiotensin converting enzyme. 
  • Evidence that direct binding of G beta gamma to the GIRK1 G protein-gated inwardly rectifying K+ channel is important for channel activation. 
  • Ex vivo adenovirus-mediated gene transfer to the adult rat heart. 
  • Exosome-Mediated Delivery of Inducible miR-423-5p Enhances Resistance of MRC-5 Cells to Rabies Virus Infection. 
  • Experimental and numerical studies of adenovirus delivery to outflow tissues of perfused human anterior segments. 
  • Expression analysis of the matrix GLA protein and VE-cadherin gene promoters in the outflow pathway. 
  • Expression of a beta-adrenergic receptor kinase 1 inhibitor prevents the development of myocardial failure in gene-targeted mice. 
  • Expression of aquaporin-1 in human trabecular meshwork cells: role in resting cell volume. 
  • Expression of functional domains of beta G-spectrin disrupts epithelial morphology in cultured cells. 
  • Expression of recombinant human methylmalonyl-CoA mutase: in primary mut fibroblasts and Saccharomyces cerevisiae. 
  • Expression of utrophin A mRNA correlates with the oxidative capacity of skeletal muscle fiber types and is regulated by calcineurin/NFAT signaling. 
  • Fetal gene transfer with lentiviral vectors: long-term in vivo follow-up evaluation in a rat model. 
  • Full genetic rescue of adenosine deaminase-deficient mice through introduction of the human gene. 
  • Full-length dystrophin restoration via targeted exon integration by AAV-CRISPR in a humanized mouse model of Duchenne muscular dystrophy. 
  • Fusigenic liposome-mediated DNA transfer into cardiac myocytes. 
  • Fusigenic viral liposome for gene therapy in cardiovascular diseases. 
  • G protein signaling and vein graft intimal hyperplasia: reduction of intimal hyperplasia in vein grafts by a Gbetagamma inhibitor suggests a major role of G protein signaling in lesion development. 
  • Gateway-compatible tissue-specific vectors for plant transformation. 
  • Gene delivery approaches to heart failure treatment. 
  • Gene delivery systems in surgery. 
  • Gene delivery to aortocoronary saphenous vein grafts in a large animal model of intimal hyperplasia. 
  • Gene inhibition and gene augmentation for the treatment of vascular proliferative disorders. 
  • Gene modification of primary tumor cells for active immunotherapy of human breast and ovarian cancer. 
  • Gene therapy and pancreatic cancer. 
  • Gene therapy for prostate cancer: where are we now? 
  • Gene therapy for the prevention of vein graft disease. 
  • Gene therapy in tissue-engineered blood vessels. 
  • Gene therapy progress and prospects: RNA aptamers. 
  • Gene therapy: role in myocardial protection. 
  • Gene transfer of endothelial nitric oxide synthase improves relaxation of carotid arteries from diabetic rabbits. 
  • Gene transfer of endothelial nitric oxide synthase reduces angiotensin II-induced endothelial dysfunction. 
  • Gene transfer to the thymus. A means of abrogating the immune response to recombinant adenovirus. 
  • Generation of neutralizing activity against human immunodeficiency virus type 1 in serum by antibody gene transfer. 
  • Generation of recombinant adeno-associated virus vectors by a complete adenovirus-mediated approach. 
  • Genetic engineering of a suboptimal islet graft with A20 preserves beta cell mass and function. 
  • Genetic therapies for cardiovascular diseases. 
  • Genetically engineered human cancer models utilizing mammalian transgene expression. 
  • Genome engineering: a new approach to gene therapy for neuromuscular disorders. 
  • Genome-editing Technologies for Gene and Cell Therapy. 
  • Glucokinase gene transfer to skeletal muscle of diabetic Zucker fatty rats improves insulin-sensitive glucose uptake. 
  • Growth inhibition of a human lung adenocarcinoma cell line by genetic complementation with chromosome 11. 
  • Herpes simplex virus (HSV)-mediated ICAM-1 gene transfer abrogates tumorigenicity and induces anti-tumor immunity. 
  • High-Pressure Transvenous Perfusion of the Upper Extremity in Human Muscular Dystrophy: A Safety Study with 0.9% Saline. 
  • High-efficiency, long-term cardiac expression of foreign genes in living mouse embryos and neonates. 
  • High-resolution labeling and functional manipulation of specific neuron types in mouse brain by Cre-activated viral gene expression. 
  • Home surveillance program prevents interstage mortality after the Norwood procedure. 
  • Human RPE expression of cell survival factors. 
  • Hyaluronic acid and fibrin hydrogels with concentrated DNA/PEI polyplexes for local gene delivery 
  • Immunity to MHC class I antigen after direct DNA transfer into skeletal muscle. 
  • Impaired proliferation and tumorigenicity induced by CCAAT/enhancer-binding protein. 
  • Improved survival and reduced phenotypic severity following AAV9/MECP2 gene transfer to neonatal and juvenile male Mecp2 knockout mice. 
  • In mice with type 2 diabetes, a vascular endothelial growth factor (VEGF)-activating transcription factor modulates VEGF signaling and induces therapeutic angiogenesis after hindlimb ischemia. 
  • In vivo gene transfer and gene modulation in hypertension research. 
  • In vivo gene transfer of nitric oxide synthase enhances vasomotor function in carotid arteries from normal and cholesterol-Fed rabbits. 
  • In vivo gene transfer to kidney by lentiviral vector. 
  • In vivo identification of a negative regulatory element in the mouse renin gene using direct gene transfer. 
  • In vivo ventricular gene delivery of a beta-adrenergic receptor kinase inhibitor to the failing heart reverses cardiac dysfunction. 
  • Incorporation of active DNA/cationic polymer polyplexes into hydrogel scaffolds 
  • Increased expression of the 5-HT6 receptor by viral mediated gene transfer into posterior but not anterior dorsomedial striatum interferes with acquisition of a discrete action-outcome task. 
  • Increased glucose disposal induced by adenovirus-mediated transfer of glucokinase to skeletal muscle in vivo. 
  • Inhibition of apoptotic signaling and neointimal hyperplasia by tempol and nitric oxide synthase following vascular injury. 
  • Interferon gamma (IFNgamma) gene transfer of an EMT6 tumor that is poorly responsive to IFNgamma stimulation: increase in tumor immunogenicity is accompanied by induction of a mouse class II transactivator and class II MHC. 
  • Intracellular third loops in AT1 and AT2 receptors determine subtype specificity. 
  • Intracerebral adeno-associated virus gene delivery of apolipoprotein E2 markedly reduces brain amyloid pathology in Alzheimer's disease mouse models. 
  • Intrastromal Gene Therapy Prevents and Reverses Advanced Corneal Clouding in a Canine Model of Mucopolysaccharidosis I. 
  • Intravital imaging of mouse embryos. 
  • Intrinsic dynamics of DNA-polymer complexes: a mechanism for DNA release. 
  • Involvement of a Rac1-Dependent Macropinocytosis Pathway in Plasmid DNA Delivery by Electrotransfection. 
  • It's All in the Delivery: Designing Hydrogels for Cell and Non-viral Gene Therapies. 
  • Lentiviral mediated gene delivery to the anterior chamber of rodent eyes. 
  • Lipophilic peptides for gene delivery. 
  • Local production of TGF beta1 inhibits cerebral edema, enhances TNF-alpha induced apoptosis and improves survival in a murine glioma model. 
  • Long-term efficacy after [E1-, polymerase-] adenovirus-mediated transfer of human acid-alpha-glucosidase gene into glycogen storage disease type II knockout mice. 
  • Low-amplitude ultrasound enhances hydrodynamic-based gene delivery to rat kidney. 
  • MAGE-1-specific precursor cytotoxic T-lymphocytes present among tumor-infiltrating lymphocytes from a patient with breast cancer: characterization and antigen-specific activation. 
  • Mapping the Structural Determinants Required for AAVrh.10 Transport across the Blood-Brain Barrier. 
  • Marked enhancement in myocardial function resulting from overexpression of a human beta-adrenergic receptor gene. 
  • Matrix-based gene delivery for tissue repair 
  • Metabolic engineering with recombinant adenoviruses. 
  • Methods for gene transfer to the central nervous system. 
  • Microcell-mediated chromosome transfer identifies EPB41L3 as a functional suppressor of epithelial ovarian cancers. 
  • Microcytic anemia in mk/mk mice is not corrected by retroviral-mediated gene transfer of wild-type p45 NF-E2. 
  • Microporous annealed particle hydrogel stiffness, void space size, and adhesion properties impact cell proliferation, cell spreading, and gene transfer. 
  • Model of creation and evolution of stable electropores for DNA delivery. 
  • Molecular engineering of the pancreatic beta-cell. 
  • Multiparameter-fluorescence activated cell sorting analysis of retroviral vector gene transfer into primitive umbilical cord blood cells. 
  • Multiple muscles in the AMD quail can be "cross-corrected" of pathologic glycogen accumulation after intravenous injection of an [E1-, polymerase-] adenovirus vector encoding human acid-alpha-glucosidase. 
  • Multiple roles for sialylated glycans in determining the cardiopulmonary tropism of adeno-associated virus 4. 
  • Myocardial injury-induced fibroblast proliferation facilitates retroviral-mediated gene transfer to the rat heart in vivo. 
  • Naked DNA delivered intraportally expresses efficiently in hepatocytes. 
  • New perspectives in hypertension research. Potentials of vascular biology. 
  • New technology and clinical applications of nanomedicine: highlights of the second annual meeting of the American Academy of Nanomedicine (Part I). 
  • Non-viral DNA delivery from porous hyaluronic acid hydrogels in mice 
  • Non-viral charge reversal vectors for pDNA delivery. 
  • Nonlinear dependence of hydraulic conductivity on tissue deformation during intratumoral infusion. 
  • Novel integrase-defective lentiviral episomal vectors for gene transfer. 
  • Novel molecular insights into RhoA GTPase-induced resistance to aqueous humor outflow through the trabecular meshwork. 
  • OB-Rb gene transfer to leptin-resistant islets reverses diabetogenic phenotype. 
  • Optimizing gene transfer to conventional outflow cells in living mouse eyes. 
  • Overexpression of G11alpha and isoforms of phospholipase C in islet beta-cells reveals a lack of correlation between inositol phosphate accumulation and insulin secretion. 
  • Overexpression of human catalase inhibits proliferation and promotes apoptosis in vascular smooth muscle cells. 
  • Overexpression of the arginine-rich carboxy-terminal region of U1 snRNP 70K inhibits both splicing and nucleocytoplasmic transport of mRNA. 
  • Persistent expression of human clotting factor IX from mouse liver after intravenous injection of adeno-associated virus vectors. 
  • Persistent, therapeutically relevant levels of human granulocyte colony-stimulating factor in mice after systemic delivery of adeno-associated virus vectors. 
  • Physical positioning markedly enhances brain transduction after intrathecal AAV9 infusion. 
  • Porous hyaluronic acid hydrogels for localized nonviral DNA delivery in a diabetic wound healing model 
  • Potential for germ line transmission after intramyocardial gene delivery by adeno-associated virus. 
  • Potentiation of beta-adrenergic signaling by adenoviral-mediated gene transfer in adult rabbit ventricular myocytes. 
  • Potentiation of beta-adrenergic signaling by gene transfer. 
  • Predicting the future of human gene therapy for cardiovascular diseases: what will the management of coronary artery disease be like in 2005 and 2010? 
  • Preservation of myocardial beta-adrenergic receptor signaling delays the development of heart failure after myocardial infarction. 
  • Prevention of restenosis by gene therapy. 
  • Production and characterization of improved adenovirus vectors with the E1, E2b, and E3 genes deleted. 
  • Production of recombinant adeno-associated viral vectors and use for in vitro and in vivo administration. 
  • Production of recombinant adeno-associated viral vectors and use in in vitro and in vivo administration. 
  • Production of recombinant adeno-associated viral vectors for in vitro and in vivo use. 
  • Production of recombinant adeno-associated viral vectors. 
  • Profound analgesia is associated with a truncated peptide resulting from tissue specific alternative splicing of DRG CA8-204 regulated by an exon-level cis-eQTL. 
  • Prostate cancer gene therapy. 
  • Protein- Polymer Nanoparticles for Nonviral Gene Delivery 
  • RNA transfected dendritic cells as cancer vaccines. 
  • RNA-transfected dendritic cells in cancer immunotherapy. 
  • Recombinant DNA Advisory Committee. Department of Health and Human Services. National Institutes of Health. Minutes of meeting. December 15-16, 1997. 
  • Redirecting migration of T cells to chemokine secreted from tumors by genetic modification with CXCR2. 
  • Reengineering a receptor footprint of adeno-associated virus enables selective and systemic gene transfer to muscle. 
  • Relaxin treatment of solid tumors: effects on electric field-mediated gene delivery. 
  • Resistance of pancreatic carcinoma cells is reversed by coculturing NK-like T cells with dendritic cells pulsed with tumor-derived RNA and CA 19-9. 
  • Resistance to adenovirally induced hyperleptinemia in rats. Comparison of ventromedial hypothalamic lesions and mutated leptin receptors. 
  • Restoration of beta-adrenergic signaling in failing cardiac ventricular myocytes via adenoviral-mediated gene transfer. 
  • Restoration of the serum level of SERPINF1 does not correct the bone phenotype in Serpinf1 null mice. 
  • Retrograde gene delivery to hypoglossal motoneurons using adeno-associated virus serotype 9. 
  • Retroviral DNA integration: viral and cellular determinants of target-site selection. 
  • Retroviral vector-mediated gene transfer into umbilical cord blood-derived megakaryocyte and platelet progenitors. 
  • Reversion mutation of cDNA CA8-204 minigene construct produces a truncated functional peptide that regulates calcium release in vitro and produces profound analgesia in vivo. 
  • Robust adenoviral and adeno-associated viral gene transfer to the in vivo murine heart: application to study of phospholamban physiology. 
  • Role for G protein-coupled receptor kinase in agonist-specific regulation of mu-opioid receptor responsiveness. 
  • Safety/feasibility of targeting the substantia nigra with AAV2-neurturin in Parkinson patients. 
  • Salmeterol with Liver Depot Gene Therapy Enhances the Skeletal Muscle Response in Murine Pompe Disease. 
  • Scaffold-mediated lentiviral transduction for functional tissue engineering of cartilage. 
  • Selective suppression of matrix metalloproteinase-9 in human glioblastoma cells by antisense gene transfer impairs glioblastoma cell invasion. 
  • Sequence variations at I260 and A1731 contribute to persistent currents in Drosophila sodium channels. 
  • Stimulus/secretion coupling factors in glucose-stimulated insulin secretion: insights gained from a multidisciplinary approach. 
  • Superoxide production in vascular smooth muscle contributes to oxidative stress and impaired relaxation in atherosclerosis. 
  • Surface-tethered DNA complexes for enhanced gene delivery 
  • Sustained correction of motoneuron histopathology following intramuscular delivery of AAV in pompe mice. 
  • Sustained release of plasmid DNA using lipid microtubules and agarose hydrogel. 
  • Synthesis and in vitro characterization of an ABC triblock copolymer for siRNA delivery 
  • Synthesis, characterization, and in vitro transfection activity of charge-reversal amphiphiles for DNA delivery. 
  • Systemic Delivery of AAVB1-GAA Clears Glycogen and Prolongs Survival in a Mouse Model of Pompe Disease. 
  • Systemic and Persistent Muscle Gene Expression in Rhesus Monkeys with a Liver De-Targeted Adeno-Associated Virus Vector. 
  • Systemic gene transfer to skeletal muscle using reengineered AAV vectors. 
  • Systemic production of human granulocyte colony-stimulating factor in nonhuman primates by transplantation of genetically modified myoblasts. 
  • Targeted entry via somatostatin receptors using a novel modified retrovirus glycoprotein that delivers genes at levels comparable to those of wild-type viral glycoproteins. 
  • Targeted gene transfer to Schlemm's canal by retroperfusion. 
  • Targeting Gbeta gamma signaling in arterial vascular smooth muscle proliferation: a novel strategy to limit restenosis. 
  • Technology evaluation: CEA-TRICOM, Therion Biologics Corp. 
  • Tethering ribozymes to a retroviral packaging signal for destruction of viral RNA. 
  • The concept and potentials of cardiovascular gene therapy. 
  • The effect of surface agitation on ultrasound-mediated gene transfer in vitro 
  • The evolving role of gene-based treatment in surgery. 
  • Therapeutic misconception in early phase gene transfer trials. 
  • Therapeutic potential of nitric oxide synthase gene manipulation. 
  • Topical application of viral vectors for epidermal gene transfer. 
  • Transdifferentiation of human endothelial progenitors into smooth muscle cells. 
  • Transfer of photosynthesis genes to and from Prochlorococcus viruses. 
  • Transmyocardial laser revascularization limits in vivo adenoviral-mediated gene transfer in porcine myocardium. 
  • Tumor-targeted gene delivery using molecularly engineered hybrid polymers functionalized with a tumor-homing peptide. 
  • Two and three-dimensional gene transfer from enzymatically degradable hydrogel scaffolds 
  • Ultrastructural Analysis of Vesicular Transport in Electrotransfection. 
  • Uncertain benefit: investigators' views and communications in early phase gene transfer trials. 
  • Use of in vivo bioluminescence imaging to predict hepatic tumor burden in mice. 
  • Use of recombinant adenovirus for metabolic engineering of mammalian cells. 
  • Using noisy gene expression mediated by engineered adenovirus to probe signaling dynamics in mammalian cells. 
  • Utilizing cell–matrix interactions to modulate gene transfer to stem cells inside hyaluronic acid hydrogels 
  • Vascular gene transfer. 
  • Vector modifications to eliminate transposase expression following piggyBac-mediated transgenesis. 
  • Viral vectors for neuronal cell type-specific visualization and manipulations. 
  • Virus-based gene therapy strategies for bone regeneration. 
  • [Experimental approaches for gene therapy modification of vascular remodeling]. 
  • [Intrathecal administration of HSV-I amplicon vector-mediated HPPE gene therapy of nocicepion in rats with formalin pain]. 
  • [Preparation of green fluorescent protein retrovirus and its application in mediating gene transfer into retinal pigment epithelial cells]. 
  • cCXCR1 is a receptor for cIL-8 (9E3/cCAF) and its N- and C-terminal peptides and is also activated by hIL-8 (CXCL8). 
  • piggyBac as a high-capacity transgenesis and gene-therapy vector in human cells and mice. 
• 

  Keywords of People

  • Berchuck, Andrew, James M. Ingram Distinguished Professor of Gynecologic Oncology, Obstetrics and Gynecology, Gynecologic Oncology
  • Chitneni, Satish K., Associate Professor in Radiology, Radiology
  • Milano, Carmelo Alessio, Joseph W. and Dorothy W. Beard Distinguished Professor of Experimental Surgery, Surgery, Cardiovascular and Thoracic Surgery
  • Sampson, John Howard, Robert H., M.D. and Gloria Wilkins Professor of Neurosurgery, in the School of Medicine, Biomedical Engineering
  • Turner, Dennis Alan, Professor of Neurosurgery, Biomedical Engineering
  • Velkey, John Matthew, Assistant Professor of the Practice of Medical Education in the Department of Cell Biology, Cell Biology