Microscopy, Atomic Force

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  Subject Areas on Research

  • A biomechanical role for perlecan in the pericellular matrix of articular cartilage. 
  • A general and efficient cantilever functionalization technique for AFM molecular recognition studies. 
  • A hybrid polymer gel with controlled rates of cross-link rupture and self-repair. 
  • A nanoscale force probe for gauging intermolecular interactions. 
  • A novel array chip to monitor in situ composite degradation using electrochemical impedance spectroscopy. 
  • A relocated technique of atomic force microscopy (AFM) samples and its application in molecular biology. 
  • A route to brightly fluorescent carbon nanotubes for near-infrared imaging in mice. 
  • A thin-layer model for viscoelastic, stress-relaxation testing of cells using atomic force microscopy: do cell properties reflect metastatic potential? 
  • Altered mechanobiology of Schlemm's canal endothelial cells in glaucoma. 
  • Altered trabecular bone structure and delayed cartilage degeneration in the knees of collagen VI null mice. 
  • An approach for fabricating self-assembled monolayer of Ag nanoparticles on gold as the SERS-active substrate. 
  • Apparent elastic modulus and hysteresis of skeletal muscle cells throughout differentiation. 
  • Atomic force and total internal reflection fluorescence microscopy for the study of force transmission in endothelial cells. 
  • Atomic force microscope investigation of large-circle DNA molecules. 
  • Atomic force microscopy captures MutS tetramers initiating DNA mismatch repair. 
  • Atomic force microscopy captures folded ribosome bound nascent chains. 
  • Atomic force microscopy captures length phenotypes in single proteins. 
  • Atomic force microscopy captures the initiation of methyl-directed DNA mismatch repair. 
  • Atomic force microscopy reveals regional variations in the micromechanical properties of the pericellular and extracellular matrices of the meniscus. 
  • Atomic levers control pyranose ring conformations. 
  • Barnacle cement: a polymerization model based on evolutionary concepts. 
  • Biocompatibility studies of functionalized regioregular poly(3-hexylthiophene) layers for sensing applications. 
  • Biomechanical effects of flow and coculture on human aortic and cord blood-derived endothelial cells. 
  • Bovine serum albumin conformation on methyl and amine functionalized surfaces compared by scanning force microscopy. 
  • Can Dissipative Properties of Single Molecules Be Extracted from a Force Spectroscopy Experiment? 
  • Capture and release of proteins on the nanoscale by stimuli-responsive elastin-like polypeptide "switches". 
  • Capturing the mechanical unfolding pathway of a large protein with coiled-coil probes. 
  • Cartilage tissue engineering using differentiated and purified induced pluripotent stem cells. 
  • Chair-boat transitions in single polysaccharide molecules observed with force-ramp AFM. 
  • Chaperones rescue luciferase folding by separating its domains. 
  • Characterization and property of DNA incorporated bilayer lipid membranes. 
  • Characterization of the adhesive plaque of the barnacle Balanus amphitrite: amyloid-like nanofibrils are a major component. 
  • Conformation change of horseradish peroxidase in lipid membrane. 
  • Construction and control of plasmid DNA network. 
  • Controlled rearrangement of adsorbed undecanol films on mica surfaces induced by an atomic force microscopy tip. 
  • Correlation between fundamental binding forces and clinical prognosis of Staphylococcus aureus infections of medical implants. 
  • DNA bioassay-on-chip using SERS detection for dengue diagnosis. 
  • DNA nanotubes self-assembled from triple-crossover tiles as templates for conductive nanowires. 
  • DNA network structures on various solid substrates investigated by atomic force microscopy. 
  • DNA sequencing: detecting methylation with force. 
  • DNA-templated self-assembly of protein and nanoparticle linear arrays. 
  • DNA-templated self-assembly of protein arrays and highly conductive nanowires. 
  • Depth-dependent anisotropy of the micromechanical properties of the extracellular and pericellular matrices of articular cartilage evaluated via atomic force microscopy. 
  • Design and construction of double-decker tile as a route to three-dimensional periodic assembly of DNA. 
  • Designing an extracellular matrix protein with enhanced mechanical stability. 
  • Detecting solvent-driven transitions of poly(A) to double-stranded conformations by atomic force microscopy. 
  • Detecting ultraviolet damage in single DNA molecules by atomic force microscopy. 
  • Direct detection of inter-residue hydrogen bonds in polysaccharides by single-molecule force spectroscopy. 
  • Direct force measurements of the streptavidin-biotin interaction. 
  • Direct measurements of base stacking interactions in DNA by single-molecule atomic-force spectroscopy. 
  • Direct observation of multimer stabilization in the mechanical unfolding pathway of a protein undergoing oligomerization. 
  • Directed nucleation assembly of DNA tile complexes for barcode-patterned lattices. 
  • Dynamic microcontact printing for patterning polymer-brush microstructures. 
  • Effect of compressive force on unbinding specific protein-ligand complexes with force spectroscopy. 
  • Elastic properties of single amylose chains in water: a quantum mechanical and AFM study. 
  • Encoded multichromophore response for simultaneous label-free detection. 
  • Endothelial, cardiac muscle and skeletal muscle exhibit different viscous and elastic properties as determined by atomic force microscopy. 
  • Envelope control of outer membrane vesicle production in Gram-negative bacteria. 
  • Enzymatic nanolithography of a self-assembled oligonucleotide monolayer on gold. 
  • Enzymatic polymerization of high molecular weight DNA amphiphiles that self-assemble into star-like micelles. 
  • Fast and forceful refolding of stretched alpha-helical solenoid proteins. 
  • Fingerprinting polysaccharides with single-molecule atomic force microscopy. 
  • Flow and high affinity binding affect the elastic modulus of the nucleus, cell body and the stress fibers of endothelial cells. 
  • Force measurements of the alpha5beta1 integrin-fibronectin interaction. 
  • Functional nanostructures from surface chemistry patterning. 
  • Growth of cationic lipid toward bilayer lipid membrane by solution spreading: scanning probe microscopy study. 
  • Human breast cancer invasion and aggression correlates with ECM stiffening and immune cell infiltration. 
  • Hydration and conformational mechanics of single, end-tethered elastin-like polypeptides. 
  • Immunofluorescence-guided atomic force microscopy to measure the micromechanical properties of the pericellular matrix of porcine articular cartilage. 
  • Improving single molecule force spectroscopy through automated real-time data collection and quantification of experimental conditions. 
  • In situ friction measurement on murine cartilage by atomic force microscopy. 
  • Inkless microcontact printing on SAMs of Boc- and TBS-protected thiols. 
  • Interaction of K7Fe3+P2W17O62H2 with supported bilayer lipid membranes on platinum electrode. 
  • Interaction of lubricin with type II collagen surfaces: adsorption, friction, and normal forces. 
  • Label-free biosensing by surface plasmon resonance of nanoparticles on glass: optimization of nanoparticle size. 
  • Label-free plasmonic detection of biomolecular binding by a single gold nanorod. 
  • Loss of cartilage structure, stiffness, and frictional properties in mice lacking PRG4. 
  • Mechanical analysis of rat trabecular meshwork. 
  • Mechanical and chemical unfolding of a single protein: a comparison. 
  • Mechanical anisotropy of ankyrin repeats. 
  • Mechanical design of proteins studied by single-molecule force spectroscopy and protein engineering. 
  • Mechanical stiffness grades metastatic potential in patient tumor cells and in cancer cell lines. 
  • Mechanical unfolding intermediates in titin modules. 
  • Mechanical unfolding of an ankyrin repeat protein. 
  • Micro- and nanostructured poly[oligo(ethylene glycol)methacrylate] brushes grown from photopatterned halogen initiators by atom transfer radical polymerization. 
  • Micromechanical mapping of early osteoarthritic changes in the pericellular matrix of human articular cartilage. 
  • Minimizing pulling geometry errors in atomic force microscope single molecule force spectroscopy. 
  • Molecular dynamics simulations of forced conformational transitions in 1,6-linked polysaccharides. 
  • Molecular stress relief through a force-induced irreversible extension in polymer contour length. 
  • Nanomechanical fingerprints of gamma radiation damage to DNA. 
  • Nanomechanics of streptavidin hubs for molecular materials. 
  • Nanopatterned polymer brushes by combining AFM anodization lithography with ring-opening metathesis polymerization in the liquid and vapor phase. 
  • Nanopatterned polymer brushes: conformation, fabrication and applications. 
  • Nanoscale detection of ionizing radiation damage to DNA by atomic force microscopy. 
  • Nanoscale structures and mechanics of barnacle cement. 
  • Nanospring behaviour of ankyrin repeats. 
  • Noncovalent functionalization of carbon nanotubes by fluorescein-polyethylene glycol: supramolecular conjugates with pH-dependent absorbance and fluorescence. 
  • Optical characterizations and electronic devices of nearly pure (10,5) single-walled carbon nanotubes. 
  • Origin of overstretching transitions in single-stranded nucleic acids. 
  • Peptide-PEG amphiphiles as cytophobic coatings for mammalian and bacterial cells. 
  • Physical constraints on charge transport through bacterial nanowires. 
  • Plasmid DNA network on a mica substrate investigated by atomic force microscopy. 
  • Point mutations alter the mechanical stability of immunoglobulin modules. 
  • Polymorphisms in fibronectin binding protein A of Staphylococcus aureus are associated with infection of cardiovascular devices. 
  • Polysaccharide elasticity governed by chair-boat transitions of the glucopyranose ring. 
  • Pretreatment of amphiphilic comb polymer surfaces dramatically affects protein adsorption. 
  • Programmable DNA self-assemblies for nanoscale organization of ligands and proteins. 
  • Pulling geometry-induced errors in single molecule force spectroscopy measurements. 
  • Regenerating titanium ventricular assist device surfaces after gold/palladium coating for scanning electron microscopy. 
  • Reverse engineering of the giant muscle protein titin. 
  • Role of fibronectin in topographical guidance of neurite extension on electrospun fibers. 
  • Self-cleaning of superhydrophobic surfaces by self-propelled jumping condensate. 
  • Separating DNA with different topologies by atomic force microscopy in comparison with gel electrophoresis. 
  • Simulating force-induced conformational transitions in polysaccharides with the SMD replica exchange method. 
  • Single molecular force spectroscopy of modular proteins in the nervous system. 
  • Single molecule mechanical manipulation for studying biological properties of proteins, DNA, and sugars. 
  • Single protein misfolding events captured by atomic force microscopy. 
  • Single-handed helical wrapping of single-walled carbon nanotubes by chiral, ionic, semiconducting polymers. 
  • Single-molecule Force Spectroscopy Reveals the Calcium Dependence of the Alternative Conformations in the Native State of a βγ-Crystallin Protein. 
  • Spatial mapping of the biomechanical properties of the pericellular matrix of articular cartilage measured in situ via atomic force microscopy. 
  • Specific binding at the cellulose binding module-cellulose interface observed by force spectroscopy. 
  • Stepwise self-assembly of DNA tile lattices using dsDNA bridges. 
  • Stretching single molecules into novel conformations using the atomic force microscope. 
  • Stretching single polysaccharides and proteins using atomic force microscopy. 
  • Structure and specificity of the RNA-guided endonuclease Cas9 during DNA interrogation, target binding and cleavage. 
  • Structure of supported bilayers composed of lipopolysaccharides and bacterial phospholipids: raft formation and implications for bacterial resistance. 
  • Surface Functionalized Graphene Biosensor on Sapphire for Cancer Cell Detection. 
  • Surface-initiated enzymatic polymerization of DNA. 
  • Surface-to-surface bridges formed by reversibly assembled polymers. 
  • Synergistic effect of high-affinity binding and flow preconditioning on endothelial cell adhesion. 
  • The force-driven conformations of heparin studied with single molecule force microscopy. 
  • The mechanical stability of ubiquitin is linkage dependent. 
  • The micro-mechanics of single molecules studied with atomic force microscopy. 
  • The molecular elasticity of the extracellular matrix protein tenascin. 
  • The relationship between ligand-binding thermodynamics and protein-ligand interaction forces measured by atomic force microscopy. 
  • The study of protein mechanics with the atomic force microscope. 
  • The surface oxidation potential of human neuromelanin reveals a spherical architecture with a pheomelanin core and a eumelanin surface. 
  • Three-helix bundle DNA tiles self-assemble into 2D lattice or 1D templates for silver nanowires. 
  • Time and distance dependence of reversible polymer bridging followed by single-molecule force spectroscopy. 
  • Tissue-culture surfaces with mixtures of aminated and fluorinated functional groups. Part 1. Synthesis and characterization. 
  • Total internal reflection microscopy and atomic force microscopy (TIRFM-AFM) to study stress transduction mechanisms in endothelial cells. 
  • UVA generates pyrimidine dimers in DNA directly. 
  • Using microcantilever deflection to detect HIV-1 envelope glycoprotein gp120. 
  • Viscoelastic properties of zonal articular chondrocytes measured by atomic force microscopy. 
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  Keywords of People

  • Bennett, Vann, George B. Geller Professor for Research in Molecular Biology in the School of Medicine, Duke Cancer Institute
  • Berchuck, Andrew, James M. Ingram Professor of Gynecologic Oncology, Obstetrics and Gynecology, Gynecologic Oncology
  • Erickson, Harold Paul, James B. Duke Professor of Medicine, Cell Biology