Transgenes

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  Subject Areas on Research

  • 4.5 kb of the rat tyrosine hydroxylase 5' flanking sequence directs tissue specific expression during development and contains consensus sites for multiple transcription factors. 
  • A gammaGT-AT1A receptor transgene protects renal cortical structure in AT1 receptor-deficient mice. 
  • A method to generate genetically defined tumors in pigs. 
  • A molecular basis for inositol polyphosphate synthesis in Drosophila melanogaster. 
  • A mouse model of adult-onset anaemia due to erythropoietin deficiency. 
  • A novel chemical screening strategy in zebrafish identifies common pathways in embryogenesis and rhabdomyosarcoma development. 
  • A novel method for viral gene delivery in solid tumors. 
  • A novel role for HEB downstream or parallel to the pre-TCR signaling pathway during alpha beta thymopoiesis. 
  • A role for histone acetylation in the developmental regulation of VDJ recombination. 
  • A small regulatory element from chromosome 19 enhances liver-specific gene expression. 
  • A statistical framework for determination of minimal plasmid copy number required for transgene expression in mammalian cells. 
  • A survey of crop-derived transgenes in activated and digester sludges in wastewater treatment plants in the United States. 
  • A threshold for central T cell tolerance to an inducible serum protein 
  • A transgenic mouse prostate cancer model. 
  • A unique role of the DNA fragmentation factor in maintaining genomic stability. 
  • Abundant expression of ponsin, a focal adhesion protein, in lens and downregulation of its expression by impaired cytoskeletal signaling. 
  • Activation of transgene expression in skeletal muscle by focused ultrasound. 
  • Acute host-mediated endothelial injury after adenoviral gene transfer in normal rabbit arteries: impact on transgene expression and endothelial function. 
  • Adaptive Evolution of the GDH2 Allosteric Domain Promotes Gliomagenesis by Resolving IDH1R132H-Induced Metabolic Liabilities. 
  • Adeno-Associated Virus Capsid-Promoter Interactions in the Brain Translate from Rat to the Nonhuman Primate. 
  • Adenoviral-mediated gene transfer induces sustained pericardial VEGF expression in dogs: effect on myocardial angiogenesis. 
  • Adenovirus-mediated genetic manipulation of the myocardial beta-adrenergic signaling system in transplanted hearts. 
  • Adsorption capacity of multiple DNA sources to clay minerals and environmental soil matrices less than previously estimated. 
  • Airway-specific inducible transgene expression using aerosolized doxycycline. 
  • Alterations in slow-twitch muscle phenotype in transgenic mice overexpressing the Ca2+ buffering protein parvalbumin. 
  • An Adeno-Associated Virus-Based Toolkit for Preferential Targeting and Manipulating Quiescent Neural Stem Cells in the Adult Hippocampus. 
  • An MYH9 human disease model in flies: site-directed mutagenesis of the Drosophila non-muscle myosin II results in hypomorphic alleles with dominant character. 
  • An efficient targeted radiotherapy/gene therapy strategy utilising human telomerase promoters and radioastatine and harnessing radiation-mediated bystander effects. 
  • Anabolic effects of a G protein-coupled receptor kinase inhibitor expressed in osteoblasts. 
  • Analysis of muscle creatine kinase regulatory elements in recombinant adenoviral vectors. 
  • Anchor cell invasion into the vulval epithelium in C. elegans. 
  • Antibody formation and mannose-6-phosphate receptor expression impact the efficacy of muscle-specific transgene expression in murine Pompe disease. 
  • BDNF regulates the maturation of inhibition and the critical period of plasticity in mouse visual cortex. 
  • Baculoviral IAP repeat-containing-4 protects optic nerve axons in a rat glaucoma model. 
  • Bcl-2 accelerates multistep prostate carcinogenesis in vivo. 
  • Bmp signaling regulates proximal-distal differentiation of endoderm in mouse lung development. 
  • Both the conserved GRAS domain and nuclear localization are required for SHORT-ROOT movement. 
  • C-myc-induced apoptosis in polycystic kidney disease is Bcl-2 and p53 independent. 
  • Calcineurin activation causes retinal ganglion cell degeneration. 
  • Catheter-based intracoronary myocardial adenoviral gene delivery: importance of intraluminal seal and infusion flow rate. 
  • Catheter-mediated subselective intracoronary gene delivery to the rabbit heart: introduction of a novel method. 
  • Cellular and functional defects in a mouse model of heart failure. 
  • Cellular cytoskeleton dynamics modulates non-viral gene delivery through RhoGTPases 
  • Characterisation of systemic dissemination of nonreplicating adenoviral vectors from tumours in local gene delivery. 
  • Characterization of a recombinant adenovirus vector encoding heat-inducible feline interleukin-12 for use in hyperthermia-induced gene-therapy. 
  • Coagulation, inflammation, and CD46 transgene expression in neonatal porcine islet xenotransplantation. 
  • Complete reversal of ischemic wall motion abnormalities by combined use of gene therapy with transmyocardial laser revascularization. 
  • Concentrated RD114-pseudotyped MFGS-gp91phox vector achieves high levels of functional correction of the chronic granulomatous disease oxidase defect in NOD/SCID/beta -microglobulin-/- repopulating mobilized human peripheral blood CD34+ cells. 
  • Conditional expression in corticothalamic efferents reveals a developmental role for nicotinic acetylcholine receptors in modulation of passive avoidance behavior. 
  • Conditional telomerase induction causes proliferation of hair follicle stem cells. 
  • Consistent production of transgenic chickens using replication-deficient retroviral vectors and high-throughput screening procedures. 
  • Construction and detection of fluorescent, germline transgenic zebrafish. 
  • Control elements within the PWS/AS imprinting box and their function in the imprinting process. 
  • Costimulation of adenylyl cyclase and phospholipase C by a mutant alpha 1B-adrenergic receptor transgene promotes malignant transformation of thyroid follicular cells. 
  • Creation of versatile cloning platforms for transgene expression and dCas9-based epigenome editing. 
  • Differential uptake of DNA–poly (ethylenimine) polyplexes in cells cultured on collagen and fibronectin surfaces 
  • Dilated cardiomyopathy in transgenic mice expressing a mutant A subunit of protein phosphatase 2A. 
  • Direct Comparison of Epifluorescence and Immunostaining for Assessing Viral Mediated Gene Expression in the Primate Brain. 
  • Discordant on/off switching of gene expression in myocytes during cardiac hypertrophy in vivo. 
  • Diverse endogenous light chains contribute to basement membrane reactivity in nonautoimmune mice transgenic for an anti-laminin Ig heavy chain. 
  • Down-regulation of the orphan nuclear receptor ROR gamma t is essential for T lymphocyte maturation. 
  • Drosophila as a model for the identification of genes causing adult human heart disease. 
  • Dystrophin immunity in Duchenne's muscular dystrophy. 
  • Effect of mutant IkappaB on cytokine-induced activation of NF-kappaB in cultured human RPE cells. 
  • Effective tumor therapy with plasmid-encoded cytokines combined with in vivo electroporation. 
  • Efficient CD4Cre-Mediated Conditional KRas Expression in Alveolar Macrophages and Alveolar Epithelial Cells Causes Fatal Hyperproliferative Pneumonitis. 
  • Engraftment of a galactose receptor footprint onto adeno-associated viral capsids improves transduction efficiency. 
  • Enhanced efficacy from gene therapy in Pompe disease using coreceptor blockade. 
  • Enhanced transduction and improved photoreceptor survival of retinal degeneration by the combinatorial use of rAAV2 with a lower dose of adenovirus. 
  • Enhancement of rAAV2-mediated transgene expression in retina cells in vitro and in vivo by coadministration of low-dose chemotherapeutic drugs. 
  • Enhancer control of local accessibility to V(D)J recombinase. 
  • Epigenetic Silencing of Recombinant Adeno-associated Virus Genomes by NP220 and the HUSH Complex. 
  • Expression of a functional antizearalenone single-chain Fv antibody in transgenic Arabidopsis plants. 
  • Expression of an antisense transforming growth factor-beta1 transgene reduces tumorigenicity of EMT6 mammary tumor cells. 
  • Expression of exogenous protein in the egg white of transgenic chickens. 
  • Expression of transgenes enriched in rare codons is enhanced by the MAPK pathway. 
  • Expression of v-Ha-ras driven by the calcitonin/calcitonin gene-related peptide promoter: a novel transgenic murine model for medullary thyroid carcinoma. 
  • Extracellular superoxide dismutase transgene overexpression preserves postischemic myocardial function in isolated murine hearts. 
  • Facilitative glucose transporter Glut1 is actively excluded from rod outer segments. 
  • Focal gene induction in the liver of rats by a heat-inducible promoter using focused ultrasound hyperthermia: preliminary results. 
  • Functional conservation of human Spastin in a Drosophila model of autosomal dominant-hereditary spastic paraplegia. 
  • Functions of E2A-HEB heterodimers in T-cell development revealed by a dominant negative mutation of HEB. 
  • G protein signaling and vein graft intimal hyperplasia: reduction of intimal hyperplasia in vein grafts by a Gbetagamma inhibitor suggests a major role of G protein signaling in lesion development. 
  • GAP-43 promoter elements in transgenic zebrafish reveal a difference in signals for axon growth during CNS development and regeneration. 
  • Gene therapy and genomic strategies for cardiovascular surgery: The emerging field of surgiomics. 
  • Gene therapy for glycogen storage diseases. 
  • Gene therapy strategy for long-term myocardial protection using adeno-associated virus-mediated delivery of heme oxygenase gene. 
  • Genetic interactions between the RhoA and Stubble-stubbloid loci suggest a role for a type II transmembrane serine protease in intracellular signaling during Drosophila imaginal disc morphogenesis. 
  • Genetically engineered human cancer models utilizing mammalian transgene expression. 
  • Genetically engineered large animal model for studying cone photoreceptor survival and degeneration in retinitis pigmentosa. 
  • Genome-wide identification of regulatory elements in Sertoli cells. 
  • HDAC4 regulates muscle fiber type-specific gene expression programs. 
  • HIV-1 Nef induces dedifferentiation of podocytes in vivo: a characteristic feature of HIVAN. 
  • HIV-1 induces renal epithelial dedifferentiation in a transgenic model of HIV-associated nephropathy. 
  • Hand2 elevates cardiomyocyte production during zebrafish heart development and regeneration. 
  • Hand2 regulates extracellular matrix remodeling essential for gut-looping morphogenesis in zebrafish. 
  • Human apolipoprotein B transgenic mice generated with 207- and 145-kilobase pair bacterial artificial chromosomes. Evidence that a distant 5'-element confers appropriate transgene expression in the intestine. 
  • Human apolipoprotein E2, E3, and E4 isoform-specific transgenic mice: human-like pattern of glial and neuronal immunoreactivity in central nervous system not observed in wild-type mice. 
  • Human gamma-satellite DNA maintains open chromatin structure and protects a transgene from epigenetic silencing. 
  • Hybrid transgenic mice reveal in vivo specificity of G protein-coupled receptor kinases in the heart. 
  • Hydrogel microsphere encapsulation of a cell-based gene therapy system increases cell survival of injected cells, transgene expression, and bone volume in a model of heterotopic ossification. 
  • Hyperactive Wnt signaling changes the developmental potential of embryonic lung endoderm. 
  • IFN-{beta} signaling positively regulates tumorigenesis in aggressive fibromatosis, potentially by modulating mesenchymal progenitors. 
  • Identification of regulators of germ stem cell enwrapment by its niche in C. elegans. 
  • Immunodominant liver-specific expression suppresses transgene-directed immune responses in murine pompe disease. 
  • Immunologic and prognostic factors associated with overall survival employing a poxviral-based PSA vaccine in metastatic castrate-resistant prostate cancer. 
  • Immunomodulatory gene therapy in lysosomal storage disorders. 
  • Impaired clearance of accumulated lysosomal glycogen in advanced Pompe disease despite high-level vector-mediated transgene expression. 
  • In vivo inhibition of elevated myocardial beta-adrenergic receptor kinase activity in hybrid transgenic mice restores normal beta-adrenergic signaling and function. 
  • In vivo monitoring of cardiomyocyte proliferation to identify chemical modifiers of heart regeneration. 
  • In vivo reprogramming of hTERT by trans-splicing ribozyme to target tumor cells. 
  • In vivo ventricular gene delivery of a beta-adrenergic receptor kinase inhibitor to the failing heart reverses cardiac dysfunction. 
  • Intrastromal Gene Therapy Prevents and Reverses Advanced Corneal Clouding in a Canine Model of Mucopolysaccharidosis I. 
  • Lack of galectin-1 or galectin-3 alters B cell deletion and anergy in an autoantibody transgene model. 
  • Lentiviral Vectors for Delivery of Gene-Editing Systems Based on CRISPR/Cas: Current State and Perspectives. 
  • Lentiviral vector design for optimal T cell receptor gene expression in the transduction of peripheral blood lymphocytes and tumor-infiltrating lymphocytes. 
  • Long-term mutagenicity studies with chloroform and dimethylnitrosamine in female lacI transgenic B6C3F1 mice. 
  • Loss of p53 sensitizes mice with a mutation in Ccm1 (KRIT1) to development of cerebral vascular malformations. 
  • MAP kinase signaling specificity mediated by the LIN-1 Ets/LIN-31 WH transcription factor complex during C. elegans vulval induction. 
  • Mapping the Structural Determinants Required for AAVrh.10 Transport across the Blood-Brain Barrier. 
  • Matrix-based gene delivery for tissue repair 
  • Mesenchymal stromal cells alone or expressing interferon-beta suppress pancreatic tumors in vivo, an effect countered by anti-inflammatory treatment. 
  • Microporous annealed particle hydrogel stiffness, void space size, and adhesion properties impact cell proliferation, cell spreading, and gene transfer. 
  • Modulation of coreceptor transcription during positive selection dictates lineage fate independently of TCR/coreceptor specificity. 
  • Modulation of tissue repair by regeneration enhancer elements. 
  • Molecular and genetic approaches for the analysis of C. elegans neuronal development. 
  • Mutational loss of PTEN induces resistance to NOTCH1 inhibition in T-cell leukemia. 
  • Non-invasive observation of repeated adenoviral GFP gene delivery to the anterior segment of the monkey eye in vivo. 
  • Normal cardiac function in mice with supraphysiological cardiac creatine levels. 
  • Novel insulinoma cell lines produced by iterative engineering of GLUT2, glucokinase, and human insulin expression. 
  • Novel integrase-defective lentiviral episomal vectors for gene transfer. 
  • Outcomes in two Japanese adenosine deaminase-deficiency patients treated by stem cell gene therapy with no cytoreductive conditioning. 
  • Overexpression of extracellular superoxide dismutase reduces acute radiation induced lung toxicity. 
  • Overexpression of human NR2B receptor subunit in LMAN causes stuttering and song sequence changes in adult zebra finches. 
  • Overexpression of the rat sarcoplasmic reticulum Ca2+ ATPase gene in the heart of transgenic mice accelerates calcium transients and cardiac relaxation. 
  • Peritubular myoid cells are not the migrating population required for testis cord formation in the XY gonad. 
  • Persistence and survival of autologous muscle derived cells versus bovine collagen as potential treatment of stress urinary incontinence. 
  • Phenotypic stability of Pro347Leu rhodopsin transgenic pigs as indicated by photoreceptor cell degeneration. 
  • Phospholipid membrane composition affects EGF receptor and Notch signaling through effects on endocytosis during Drosophila development. 
  • Physiological induction of a beta-adrenergic receptor kinase inhibitor transgene preserves ss-adrenergic responsiveness in pressure-overload cardiac hypertrophy. 
  • Podocan, a novel small leucine-rich repeat protein expressed in the sclerotic glomerular lesion of experimental HIV-associated nephropathy. 
  • Polyclonal long-term MFGS-gp91phox marking in rhesus macaques after nonmyeloablative transplantation with transduced autologous peripheral blood progenitor cells. 
  • Potential for germ line transmission after intramyocardial gene delivery by adeno-associated virus. 
  • Potentiation of beta-adrenergic signaling by adenoviral-mediated gene transfer in adult rabbit ventricular myocytes. 
  • Potentiation of diethylstilbestrol-induced alterations in the female mouse reproductive tract by transforming growth factor-alpha transgene expression. 
  • Preclinical Development of New Therapy for Glycogen Storage Diseases. 
  • Preclinical efficacy of MEK inhibition in Nras-mutant AML. 
  • Predicting longevity in C. elegans: fertility, mobility and gene expression. 
  • Presynaptic Inputs to Any CNS Projection Neuron Identified by Dual Recombinant Virus Infection. 
  • Pronounced thrombocytosis in transgenic mice expressing reduced levels of Mpl in platelets and terminally differentiated megakaryocytes. 
  • Prostate intraepithelial neoplasia induced by prostate restricted Akt activation: the MPAKT model. 
  • Quantitative genetic variation in CD19 expression correlates with autoimmunity. 
  • RacGap50C negatively regulates wingless pathway activity during Drosophila embryonic development. 
  • Rapid and Efficient Generation of Transgene-Free iPSC from a Small Volume of Cryopreserved Blood. 
  • Recombinant human transgenic antithrombin in cardiac surgery: a dose-finding study. 
  • Recombinant oncolytic poliovirus, PVSRIPO, has potent cytotoxic and innate inflammatory effects, mediating therapy in human breast and prostate cancer xenograft models. 
  • Redirecting cytotoxic T lymphocyte responses with T-cell receptor transgenes. 
  • Regulation of mitochondrial biogenesis in skeletal muscle by CaMK. 
  • Regulation of zebrafish heart regeneration by miR-133. 
  • Relaxed negative selection in germinal centers and impaired affinity maturation in bcl-xL transgenic mice. 
  • Relaxin treatment of solid tumors: effects on electric field-mediated gene delivery. 
  • Remodeling of the Acetylproteome by SIRT3 Manipulation Fails to Affect Insulin Secretion or β Cell Metabolism in the Absence of Overnutrition. 
  • Repression of B7.2 on self-reactive B cells is essential to prevent proliferation and allow Fas-mediated deletion by CD4(+) T cells. 
  • Repurposing an endogenous degradation system for rapid and targeted depletion of C. elegans proteins. 
  • Restoration of beta-adrenergic signaling in failing cardiac ventricular myocytes via adenoviral-mediated gene transfer. 
  • Restoring striatal WAVE-1 improves maze exploration performance of GluN1 knockdown mice. 
  • Retinoic acid-mediated gene expression in transgenic reporter zebrafish. 
  • Retrograde influence of muscle fibers on their innervation revealed by a novel marker for slow motoneurons. 
  • Reversal of diet-induced glucose intolerance by hepatic expression of a variant glycogen-targeting subunit of protein phosphatase-1. 
  • Rhesus immune responses to SIV Gag expressed by recombinant BCG vectors are independent from pre-existing mycobacterial immunity. 
  • Rho GDP dissociation inhibitor-mediated disruption of Rho GTPase activity impairs lens fiber cell migration, elongation and survival. 
  • Right ventricular gene therapy with a beta-adrenergic receptor kinase inhibitor improves survival after pulmonary artery banding. 
  • Robust Transgene Expression from Bicistronic mRNA in the Green Alga Chlamydomonas reinhardtii. 
  • Role for the target enzyme in deactivation of photoreceptor G protein in vivo. 
  • Signaling role of hemocytes in Drosophila JAK/STAT-dependent response to septic injury. 
  • Sox2 expression in Schwann cells inhibits myelination in vivo and induces influx of macrophages to the nerve. 
  • Spindle dynamics during meiosis in Drosophila oocytes. 
  • Structure-guided reprogramming of serine recombinase DNA sequence specificity. 
  • Synthesis of programmable integrases. 
  • Systemic dissemination of viral vectors during intratumoral injection. 
  • Systemic vector leakage and transgene expression by intratumorally injected recombinant adenovirus vectors. 
  • Targeted activation of c-Jun N-terminal kinase in vivo induces restrictive cardiomyopathy and conduction defects. 
  • Targeted expression of BikDD eradicates pancreatic tumors in noninvasive imaging models. 
  • Targeting cells with single vectors using multiple-feature Boolean logic. 
  • Targeting proximal tubule mitochondrial dysfunction attenuates the renal disease of methylmalonic acidemia. 
  • Targeting the receptor-Gq interface to inhibit in vivo pressure overload myocardial hypertrophy. 
  • The Macrophage-Specific Promoter mfap4 Allows Live, Long-Term Analysis of Macrophage Behavior during Mycobacterial Infection in Zebrafish. 
  • The upstream enhancer elements of the G6PC promoter are critical for optimal G6PC expression in murine glycogen storage disease type Ia. 
  • Transfection efficiency and transgene expression kinetics of mRNA delivered in naked and nanoparticle format. 
  • Transfer of CD8+ T cell memory using Bcl-2 as a marker. 
  • Transgene induced co-suppression during vegetative growth in Cryptococcus neoformans. 
  • Transgenic expression of green fluorescence protein can cause dilated cardiomyopathy. 
  • Transgenic expression of pancreatic secretory trypsin inhibitor-1 rescues SPINK3-deficient mice and restores a normal pancreatic phenotype. 
  • Transgenic expression of pancreatic secretory trypsin inhibitor-I ameliorates secretagogue-induced pancreatitis in mice. 
  • Trastuzumab signaling in ErbB2-overexpressing inflammatory breast cancer correlates with X-linked inhibitor of apoptosis protein expression. 
  • Troponin T3 expression in skeletal and smooth muscle is required for growth and postnatal survival: characterization of Tnnt3(tm2a(KOMP)Wtsi) mice. 
  • Unphosphorylated STAT3 modulates alpha 7 nicotinic receptor signaling and cytokine production in sepsis. 
  • Using rAAV2-retro in rhesus macaques: Promise and caveats for circuit manipulation. 
  • Utilizing an interim futility analysis of the OVAL study (VB-111-701/GOG 3018) for potential reduction of risk: A phase III, double blind, randomized controlled trial of ofranergene obadenovec (VB-111) and weekly paclitaxel in patients with platinum resistant ovarian cancer. 
  • Utilizing cell–matrix interactions to modulate gene transfer to stem cells inside hyaluronic acid hydrogels 
  • Viral sequences enable efficient and tissue-specific expression of transgenes in Xenopus. 
  • Whole-organism screening for gluconeogenesis identifies activators of fasting metabolism. 
  • [Investigation of neuroprotective activity of apolipoprotein E peptide mimetic Cog1410 in transgenic lines of Drosophila melanogaster]. 
  • p38-MAPK induced dephosphorylation of alpha-tropomyosin is associated with depression of myocardial sarcomeric tension and ATPase activity. 
  • piggyBac as a high-capacity transgenesis and gene-therapy vector in human cells and mice. 
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  Keywords of People

  • Bejsovec, Amy, Associate Professor of Biology, Biology
  • Ciofani, Maria, Associate Professor of Integrative Immunobiology, Cell Biology
  • Hogan, Brigid L. M., Research Professor of Cell Biology, Cell Biology