Biolistics

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  Subject Areas on Research

  • Cryptococcus neoformans virulence gene discovery through insertional mutagenesis. 
  • Development of a low-pressure microtargeting biolistic device for transfection of retinal explants. 
  • Gene disruption by biolistic transformation in serotype D strains of Cryptococcus neoformans. 
  • Increased expression of brain-derived neurotrophic factor induces formation of basal dendrites and axonal branching in dentate granule cells in hippocampal explant cultures. 
  • Neuronal transfection using particle-mediated gene transfer. 
  • Recapitulation of the sexual cycle of the primary fungal pathogen Cryptococcus neoformans var. gattii: implications for an outbreak on Vancouver Island, Canada. 
  • Screening of HIV-1 Env glycoproteins for the ability to raise neutralizing antibody using DNA immunization and recombinant vaccinia virus boosting. 
  • Study of Cryptococcus neoformans actin gene regulation with a beta-galactosidase-actin fusion. 
  • The glitter of gold: biolistic transfection of fresh adult cardiac myocytes. Focus on "normal targeting of a tagged Kv1.5 channel acutely transfected into fresh adult cardiac myocytes by a biolistic method". 
  • Transgenic manipulation of the metabolism of polyamines in poplar cells.