Streptavidin

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  Subject Areas on Research

  • A colorimetric gold nanoparticle sensor to interrogate biomolecular interactions in real time on a surface. 
  • Astatine-211-labeled biotin conjugates resistant to biotinidase for use in pretargeted radioimmunotherapy. 
  • Binding and signaling of surface-immobilized reagentless fluorescent biosensors derived from periplasmic binding proteins. 
  • Complications in cell-surface labelling by biotinylation of Candida albicans due to avidin conjugate binding to cell-wall proteins. 
  • Control of protein-ligand recognition using a stimuli-responsive polymer. 
  • Cutting edge: C3d functions as a molecular adjuvant in the absence of CD21/35 expression. 
  • DNA-templated self-assembly of protein and nanoparticle linear arrays. 
  • DNA-templated self-assembly of protein arrays and highly conductive nanowires. 
  • Detection of epitope-tagged proteins in flow cytometry: fluorescence resonance energy transfer-based assays on beads with femtomole resolution. 
  • Differential osteopontin expression in phenotypically distinct subclones of murine breast cancer cells mediates metastatic behavior. 
  • Direct force measurements of the streptavidin-biotin interaction. 
  • Direct observation of multimer stabilization in the mechanical unfolding pathway of a protein undergoing oligomerization. 
  • Distinct MutS DNA-binding modes that are differentially modulated by ATP binding and hydrolysis. 
  • Dual-order snapshot spectral imaging of plasmonic nanoparticles. 
  • Dynamic seeding of perfusing human umbilical vein endothelial cells (HUVECs) onto dual-function cell adhesion ligands: Arg-Gly-Asp (RGD)-streptavidin and biotinylated fibronectin. 
  • Effect of streptavidin RGD mutant on the adhesion of endothelial cells. 
  • Effect of streptavidin affinity mutants on the integrin-independent adhesion of biotinylated endothelial cells. 
  • Effect of streptavidin-biotin on endothelial vasoregulation and leukocyte adhesion. 
  • Elastomeric microparticles for acoustic mediated bioseparations. 
  • Electrochemical biosensor of nanocube-augmented carbon nanotube networks. 
  • Engineered chimeric streptavidin tetramers as novel tools for bioseparations and drug delivery. 
  • Enhanced TOF-SIMS imaging of a micropatterned protein by stable isotope protein labeling. 
  • Founder's Award, Society for Biomaterials. Sixth World Biomaterials Congress 2000, Kamuela, HI,May 15-20, 2000. Really smart bioconjugates of smart polymers and receptor proteins. 
  • Helical crystallization on lipid nanotubes: streptavidin as a model protein. 
  • High-affinity augmentation of endothelial cell attachment: long-term effects on focal contact and actin filament formation. 
  • High-throughput screen for inhibitors of 1-deoxy-d-xylulose 5-phosphate reductoisomerase by surrogate ligand competition. 
  • Label-free plasmonic detection of biomolecular binding by a single gold nanorod. 
  • Molecular engineering of proteins and polymers for targeting and intracellular delivery of therapeutics. 
  • Nanomechanics of streptavidin hubs for molecular materials. 
  • Peptides, antibodies, and FRET on beads in flow cytometry: A model system using fluoresceinated and biotinylated beta-endorphin. 
  • Plasmonic detection of a model analyte in serum by a gold nanorod sensor. 
  • Preparation and characterization of anti-tenascin monoclonal antibody-streptavidin conjugates for pretargeting applications. 
  • Rational selection of gold nanorod geometry for label-free plasmonic biosensors. 
  • Site-directed mutagenesis studies of the high-affinity streptavidin-biotin complex: contributions of tryptophan residues 79, 108, and 120. 
  • Small-volume rapid-mix device for subsecond kinetic analysis in flow cytometry. 
  • Spectroscopic characterization of streptavidin functionalized quantum dots. 
  • Streptavidin binding and endothelial cell adhesion to biotinylated fibronectin. 
  • Streptavidin-biotin binding energetics. 
  • Structural studies of binding site tryptophan mutants in the high-affinity streptavidin-biotin complex. 
  • Synergistic effect of shear stress and streptavidin-biotin on the expression of endothelial vasodilator and cytoskeleton genes. 
  • Synthesis and preliminary biological evaluation of (3-iodobenzoyl)norbiotinamide and ((5-iodo-3-pyridinyl)carbonyl)norbiotinamide: two radioiodinated biotin conjugates with improved stability. 
  • The development of quantum dot calibration beads and quantitative multicolor bioassays in flow cytometry and microscopy. 
  • The motion of a single molecule, the lambda-receptor, in the bacterial outer membrane. 
  • The relationship between ligand-binding thermodynamics and protein-ligand interaction forces measured by atomic force microscopy. 
  • Three-step radioimmunotherapy with yttrium-90 biotin: dosimetry and pharmacokinetics in cancer patients. 
  • Two-dimensional protein crystallization via metal-ion coordination by naturally occurring surface histidines. 
  • X-ray crystallographic studies of streptavidin mutants binding to biotin. 
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  Keywords of People

  • Bennett, Vann, George B. Geller Professor for Research in Molecular Biology in the School of Medicine, Duke Cancer Institute