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Subject Areas on Research
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2.2 Å resolution cryo-EM structure of β-galactosidase in complex with a cell-permeant inhibitor.
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A Novel Phosphoregulatory Switch Controls the Activity and Function of the Major Catalytic Subunit of Protein Kinase A in Aspergillus fumigatus.
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A Sch9 protein kinase homologue controlling virulence independently of the cAMP pathway in Cryptococcus neoformans.
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A conserved domain for glycogen binding in protein phosphatase-1 targeting subunits.
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A conserved family of enzymes that phosphorylate inositol hexakisphosphate.
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A core-weighted fitting method for docking atomic structures into low-resolution maps: application to cryo-electron microscopy.
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A genetically tractable model of human glioma formation.
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A high affinity, antidote-controllable prothrombin and thrombin-binding RNA aptamer inhibits thrombin generation and thrombin activity.
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A minimal TrpRS catalytic domain supports sense/antisense ancestry of class I and II aminoacyl-tRNA synthetases.
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A protein encoded within the Down syndrome critical region is enriched in striated muscles and inhibits calcineurin signaling.
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A single step purification for autolytic zinc proteinases.
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A structural basis for lithium and substrate binding of an inositide phosphatase.
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Accurate Quantum Mechanical/Molecular Mechanical Calculations of Reduction Potentials in Azurin Variants.
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Activity and stability of recombinant bifunctional rearranged and monofunctional domains of ATP-sulfurylase and adenosine 5'-phosphosulfate kinase.
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Allosteric effects of external K+ ions mediated by the aspartate of the GYGD signature sequence in the Kv2.1 K+ channel.
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Altered nucleotide-microtubule coupling and increased mechanical output by a kinesin mutant.
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Angiostatin-like activity of a monoclonal antibody to the catalytic subunit of F1F0 ATP synthase.
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Anti-inflammatory and vasoprotective activity of a retroviral-derived peptide, homologous to human endogenous retroviruses: endothelial cell effects.
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Atomistic basis of force generation, translocation, and coordination in a viral genome packaging motor.
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Automated image acquisition and processing using a new generation of 4K x 4K CCD cameras for cryo electron microscopic studies of macromolecular assemblies.
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Branched-Chain Aminotransferases Control TORC1 Signaling in Saccharomyces cerevisiae.
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C-terminal regions of the human telomerase catalytic subunit essential for in vivo enzyme activity.
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Cell-type-dependent histone demethylase specificity promotes meiotic chromosome condensation in Arabidopsis.
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Cellular mechanisms regulating protein phosphatase-1. A key functional interaction between inhibitor-2 and the type 1 protein phosphatase catalytic subunit.
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Characterization of SARS2 Nsp15 nuclease activity reveals it's mad about U.
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Characterization of key residues in the subdomain encoded by exons 8 and 9 of human inducible nitric oxide synthase: a critical role for Asp-280 in substrate binding and subunit interactions.
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Characterization of the CaMKKβ-AMPK signaling complex.
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Choline Acetyltransferase Mutations Causing Congenital Myasthenic Syndrome: Molecular Findings and Genotype-Phenotype Correlations.
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Computational design of a biologically active enzyme.
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Conformation-assisted inhibition of protein-tyrosine phosphatase-1B elicits inhibitor selectivity over T-cell protein-tyrosine phosphatase.
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Cryo-EM reveals active site coordination within a multienzyme pre-rRNA processing complex.
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Cryo-EM structures of the SARS-CoV-2 endoribonuclease Nsp15 reveal insight into nuclease specificity and dynamics.
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Crystal structure of MraY, an essential membrane enzyme for bacterial cell wall synthesis.
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Crystal structure of Streptococcus pyogenes sortase A: implications for sortase mechanism.
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Crystal structure of the MOP flippase MurJ in an inward-facing conformation.
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Crystal structures of the fungal pathogen Aspergillus fumigatus protein farnesyltransferase complexed with substrates and inhibitors reveal features for antifungal drug design.
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Cyclic AMP-dependent protein kinase catalytic subunits have divergent roles in virulence factor production in two varieties of the fungal pathogen Cryptococcus neoformans.
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Cyclic AMP-dependent protein kinase controls virulence of the fungal pathogen Cryptococcus neoformans.
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Cyclic AMP-dependent protein kinase regulates pseudohyphal differentiation in Saccharomyces cerevisiae.
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Defects in RGS9 or its anchor protein R9AP in patients with slow photoreceptor deactivation.
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Design, synthesis, and biologic evaluation of novel galloyl derivatives as HIV-1 RNase H inhibitors.
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Determination of separate inhibitor and substrate binding sites in the dehaloperoxidase-hemoglobin from Amphitrite ornata.
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Development of potent B-RafV600E inhibitors containing an arylsulfonamide headgroup.
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Direct allosteric regulation between the GAF domain and catalytic domain of photoreceptor phosphodiesterase PDE6.
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Direct evidence for methyl group coordination by carbon-oxygen hydrogen bonds in the lysine methyltransferase SET7/9.
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Directed evolution of a new catalytic site in 2-keto-3-deoxy-6-phosphogluconate aldolase from Escherichia coli.
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Distinct Regions of the Haloferax volcanii Dolichol Phosphate-Mannose Synthase AglD Mediate the Assembly and Subsequent Processing of the Lipid-Linked Mannose.
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Divergence of protein kinase A catalytic subunits in Cryptococcus neoformans and Cryptococcus gattii illustrates evolutionary reconfiguration of a signaling cascade.
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Drosophila stretchin-MLCK is a novel member of the Titin/Myosin light chain kinase family.
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Emerging applications of aptamers for anticoagulation and hemostasis.
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Environmental Effects on Guanine-Thymine Mispair Tautomerization Explored with Quantum Mechanical/Molecular Mechanical Free Energy Simulations.
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Enzymatic properties of ALDH1L2, a mitochondrial 10-formyltetrahydrofolate dehydrogenase.
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Enzyme redesign guided by cancer-derived IDH1 mutations.
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Experimentally Dissecting the Origins of Peroxiredoxin Catalysis.
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From promiscuity to precision: protein phosphatases get a makeover.
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Functional Class I and II Amino Acid-activating Enzymes Can Be Coded by Opposite Strands of the Same Gene.
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Heme-assisted S-nitrosation desensitizes ferric soluble guanylate cyclase to nitric oxide.
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HipBA-promoter structures reveal the basis of heritable multidrug tolerance.
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Identification and characterization of a highly conserved calcineurin binding protein, CBP1/calcipressin, in Cryptococcus neoformans.
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Identification of casein kinase I substrates by in vitro expression cloning screening.
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Identification of cellular protein phosphatase-1 regulators.
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Identification of key amino acids responsible for the substantially higher affinities of human type 1 3beta-hydroxysteroid dehydrogenase/isomerase (3beta-HSD1) for substrates, coenzymes, and inhibitors relative to human 3beta-HSD2.
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Importance of a surface hydrophobic pocket on protein phosphatase-1 catalytic subunit in recognizing cellular regulators.
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Improving SARS-CoV-2 structures: Peer review by early coordinate release.
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Improving upon nature: active site remodeling produces highly efficient aldolase activity toward hydrophobic electrophilic substrates.
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Initiating cellular stress responses.
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Insights into the Autoproteolytic Processing and Catalytic Mechanism of the Chlamydia trachomatis Virulence-Associated Protease CPAF.
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Interplay of catalysis, fidelity, threading, and processivity in the exo- and endonucleolytic reactions of human exonuclease I.
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Intrinsic GTP hydrolysis is observed for a switch 1 variant of Cdc42 in the presence of a specific GTPase inhibitor.
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Involvement of the beta clamp in methyl-directed mismatch repair in vitro.
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Isocitrate-to-SENP1 signaling amplifies insulin secretion and rescues dysfunctional β cells.
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Mapping hole hopping escape routes in proteins.
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Masitinib is a broad coronavirus 3CL inhibitor that blocks replication of SARS-CoV-2.
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Mcm2-7 Is an Active Player in the DNA Replication Checkpoint Signaling Cascade via Proposed Modulation of Its DNA Gate.
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Mechanism of OMP decarboxylation in orotidine 5'-monophosphate decarboxylase.
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Mechanism of Reduction of an Aminyl Radical Intermediate in the Radical SAM GTP 3',8-Cyclase MoaA.
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Mechanistic insights into cancer cell killing through interaction of phosphodiesterase 3A and schlafen family member 12.
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Missense and truncating variants in CHD5 in a dominant neurodevelopmental disorder with intellectual disability, behavioral disturbances, and epilepsy.
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Molecular cloning and characterization of a novel histone deacetylase HDAC10.
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Molecular determinants of nuclear protein phosphatase-1 regulation by NIPP-1.
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Molecular mechanism of ERK dephosphorylation by striatal-enriched protein tyrosine phosphatase.
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Multiple interactions within the AKAP220 signaling complex contribute to protein phosphatase 1 regulation.
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Mutational analysis of active site residues in the Staphylococcus aureus transpeptidase SrtA.
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Mutational analysis of the tyrosine phosphatome in colorectal cancers.
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Mutations in hemG mediate resistance to salicylidene acylhydrazides, demonstrating a novel link between protoporphyrinogen oxidase (HemG) and Chlamydia trachomatis infectivity.
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N-terminal domains of the human telomerase catalytic subunit required for enzyme activity in vivo.
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NMR and XAS reveal an inner-sphere metal binding site in the P4 helix of the metallo-ribozyme ribonuclease P.
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Neurabins recruit protein phosphatase-1 and inhibitor-2 to the actin cytoskeleton.
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Nucleotide regulation of soluble guanylate cyclase substrate specificity.
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Oxalate decarboxylase uses electron hole hopping for catalysis.
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Oxidative stress-induced expression and modulation of Phosphatase of Regenerating Liver-1 (PRL-1) in mammalian retina.
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Perturbation of fuel homeostasis caused by overexpression of the glucose-6-phosphatase catalytic subunit in liver of normal rats.
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Phosphorylation of the myosin phosphatase target subunit by integrin-linked kinase.
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Photo-activated psoralen binds the ErbB2 catalytic kinase domain, blocking ErbB2 signaling and triggering tumor cell apoptosis.
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Potent anticoagulant aptamer directed against factor IXa blocks macromolecular substrate interaction.
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Probing domain interactions in soluble guanylate cyclase.
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Probing the architecture of the B. subtilis RNase P holoenzyme active site by cross-linking and affinity cleavage.
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Prolonged Adaptive Evolution of a Defensive Gene in the Solanaceae.
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Protein kinase A operates a molecular switch that governs yeast pseudohyphal differentiation.
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Protein phosphatase 2A and its B56 regulatory subunit inhibit Wnt signaling in Xenopus.
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Protein serine/threonine phosphatases: life, death, and sleeping.
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Putative telomere-recruiting domain in the catalytic subunit of human telomerase.
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Role of the amino terminal RHAU-specific motif in the recognition and resolution of guanine quadruplex-RNA by the DEAH-box RNA helicase RHAU.
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Sirtuin 3 (SIRT3) protein regulates long-chain acyl-CoA dehydrogenase by deacetylating conserved lysines near the active site.
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Site-specific incorporation of 3-nitrotyrosine as a probe of pKa perturbation of redox-active tyrosines in ribonucleotide reductase.
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Species-specific and inhibitor-dependent conformations of LpxC: implications for antibiotic design.
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Specific keynote: genome copy number abnormalities in ovarian cancer.
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Specificity determinants in phosphoinositide dephosphorylation: crystal structure of an archetypal inositol polyphosphate 5-phosphatase.
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Statistical evaluation of the Rodin-Ohno hypothesis: sense/antisense coding of ancestral class I and II aminoacyl-tRNA synthetases.
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Structural and In Vivo Studies on Trehalose-6-Phosphate Synthase from Pathogenic Fungi Provide Insights into Its Catalytic Mechanism, Biological Necessity, and Potential for Novel Antifungal Drug Design.
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Structural insights into inhibition of lipid I production in bacterial cell wall synthesis.
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Structural model of human PORCN illuminates disease-associated variants and drug-binding sites.
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Structural reorganization and preorganization in enzyme active sites: comparisons of experimental and theoretically ideal active site geometries in the multistep serine esterase reaction cycle.
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Structure of the LpxC deacetylase with a bound substrate-analog inhibitor.
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Structure of the manganese-bound manganese transport regulator of Bacillus subtilis.
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Structure of β-galactosidase at 3.2-Å resolution obtained by cryo-electron microscopy.
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Structure-based design and synthesis of potent, ethylenediamine-based, mammalian farnesyltransferase inhibitors as anticancer agents.
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Structures of Pathogenic Fungal FKBP12s Reveal Possible Self-Catalysis Function.
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Suppression of Rev3, the catalytic subunit of Pol{zeta}, sensitizes drug-resistant lung tumors to chemotherapy.
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Synthesis of programmable integrases.
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The Closing Mechanism of DNA Polymerase I at Atomic Resolution.
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The DNA binding domain of a papillomavirus E2 protein programs a chimeric nuclease to cleave integrated human papillomavirus DNA in HeLa cervical carcinoma cells.
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The Escherichia coli subtilase cytotoxin A subunit specifically cleaves cell-surface GRP78 protein and abolishes COOH-terminal-dependent signaling.
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The Rodin-Ohno hypothesis that two enzyme superfamilies descended from one ancestral gene: an unlikely scenario for the origins of translation that will not be dismissed.
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The catalytic region and PEST domain of PTPN18 distinctly regulate the HER2 phosphorylation and ubiquitination barcodes.
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The cis-Pro touch-turn: a rare motif preferred at functional sites.
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The clash in titin.
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The crystal structure of the catalytic domain of a eukaryotic guanylate cyclase.
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The deubiquitinases USP33 and USP20 coordinate beta2 adrenergic receptor recycling and resensitization.
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The fidelity of DNA replication, particularly on GC-rich templates, is reduced by defects of the Fe-S cluster in DNA polymerase δ.
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The gain of rod phototransduction: reconciliation of biochemical and electrophysiological measurements.
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The mechanism of discrimination between oxidized and reduced coenzyme in the aldehyde dehydrogenase domain of Aldh1l1.
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The nucleolar localization domain of the catalytic subunit of human telomerase.
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The promoter for the gene encoding the catalytic subunit of rat glucose-6-phosphatase contains two distinct glucose-responsive regions.
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The structural basis for the selectivity of benzotriazole inhibitors of PTP1B.
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The structure of formylmethanofuran: tetrahydromethanopterin formyltransferase in complex with its coenzymes.
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The tumor suppressor folliculin inhibits lactate dehydrogenase A and regulates the Warburg effect.
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The ubiquitin-conjugating enzyme (E2) Ube2w ubiquitinates the N terminus of substrates.
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beta-arrestin1 interacts with the catalytic domain of the tyrosine kinase c-SRC. Role of beta-arrestin1-dependent targeting of c-SRC in receptor endocytosis.
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trans-2-Phenylcyclopropylamine is a mechanism-based inactivator of the histone demethylase LSD1.
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Keywords of People
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Berchuck, Andrew,
James M. Ingram Distinguished Professor of Gynecologic Oncology,
Obstetrics and Gynecology, Gynecologic Oncology
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Erickson, Harold Paul,
Professor Emeritus of Cell Biology,
Cell Biology
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Sampson, John Howard,
Robert H., M.D. and Gloria Wilkins Professor of Neurosurgery, in the School of Medicine,
Biomedical Engineering
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Yan, Hai,
Adjunct Professor of Pathology,
Pathology