Cryoelectron Microscopy

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  Subject Areas on Research

  • 2.2 Å resolution cryo-EM structure of β-galactosidase in complex with a cell-permeant inhibitor. 
  • 2.3 Å resolution cryo-EM structure of human p97 and mechanism of allosteric inhibition. 
  • A 3.8 Å resolution cryo-EM structure of a small protein bound to an imaging scaffold. 
  • A collaborative framework for 3D alignment and classification of heterogeneous subvolumes in cryo-electron tomography. 
  • A core-weighted fitting method for docking atomic structures into low-resolution maps: application to cryo-electron microscopy. 
  • A new way to see RNAs. 
  • Activation mechanism of the mouse cold-sensing TRPM8 channel by cooling agonist and PIP2. 
  • An HIV-1 antibody from an elite neutralizer implicates the fusion peptide as a site of vulnerability. 
  • Assessment of detailed conformations suggests strategies for improving cryoEM models: Helix at lower resolution, ensembles, pre-refinement fixups, and validation at multi-residue length scale. 
  • Atomic Resolution Cryo-EM Structure of β-Galactosidase. 
  • Atomic resolution cryo-EM structure of a native-like CENP-A nucleosome aided by an antibody fragment. 
  • Automated image acquisition and processing using a new generation of 4K x 4K CCD cameras for cryo electron microscopic studies of macromolecular assemblies. 
  • Automated systematic evaluation of cryo-EM specimens with SmartScope. 
  • Beam image-shift accelerated data acquisition for near-atomic resolution single-particle cryo-electron tomography. 
  • Bespoke library docking for 5-HT2A receptor agonists with antidepressant activity. 
  • Breaking Cryo-EM Resolution Barriers to Facilitate Drug Discovery. 
  • Caging metal ions with visible light-responsive nanopolymersomes. 
  • Characterization of SARS2 Nsp15 nuclease activity reveals it's mad about U. 
  • Classification of polyhedral shapes from individual anisotropically resolved cryo-electron tomography reconstructions. 
  • Coevolution of Adeno-associated Virus Capsid Antigenicity and Tropism through a Structure-Guided Approach. 
  • Cold sensitivity of the SARS-CoV-2 spike ectodomain. 
  • Complementary antibody lineages achieve neutralization breadth in an HIV-1 infected elite neutralizer. 
  • Computational separation of conformational heterogeneity using cryo-electron tomography and 3D sub-volume averaging. 
  • Conformational ensemble of the human TRPV3 ion channel. 
  • Constructing atomic structural models into cryo-EM densities using molecular dynamics - Pros and cons. 
  • Controlling the SARS-CoV-2 spike glycoprotein conformation. 
  • Cryo-EM Analysis of the Conformational Landscape of Human P-glycoprotein (ABCB1) During its Catalytic Cycle. 
  • Cryo-EM Structures Reveal Mechanism and Inhibition of DNA Targeting by a CRISPR-Cas Surveillance Complex. 
  • Cryo-EM Structures of the Magnesium Channel CorA Reveal Symmetry Break upon Gating. 
  • Cryo-EM model validation recommendations based on outcomes of the 2019 EMDataResource challenge. 
  • Cryo-EM reveals active site coordination within a multienzyme pre-rRNA processing complex. 
  • Cryo-EM reveals the architecture of the PELP1-WDR18 molecular scaffold. 
  • Cryo-EM structure of a mitochondrial calcium uniporter. 
  • Cryo-EM structure of human rhodopsin bound to an inhibitory G protein. 
  • Cryo-EM structure of the essential ribosome assembly AAA-ATPase Rix7. 
  • Cryo-EM structures of SARS-CoV-2 Omicron BA.2 spike. 
  • Cryo-EM structures of the SARS-CoV-2 endoribonuclease Nsp15 reveal insight into nuclease specificity and dynamics. 
  • Cryo-electron Microscopy Structures of Chimeric Hemagglutinin Displayed on a Universal Influenza Vaccine Candidate. 
  • Cryo-electron microscopy structure of the TRPV2 ion channel. 
  • Cryo-electron microscopy structure of the lysosomal calcium-permeable channel TRPML3. 
  • Cryo-electron microscopy--a primer for the non-microscopist. 
  • Current View of Ligand and Lipid Recognition by the Menthol Receptor TRPM8. 
  • D614G Mutation Alters SARS-CoV-2 Spike Conformation and Enhances Protease Cleavage at the S1/S2 Junction. 
  • Data-driven determination of number of discrete conformations in single-particle cryo-EM. 
  • Dengue virus and autophagy. 
  • Determination of molecular structures of HIV envelope glycoproteins using cryo-electron tomography and automated sub-tomogram averaging. 
  • Disc formation in cholesterol-free liposomes during phase transition. 
  • Discrete fracture patterns of virus shells reveal mechanical building blocks. 
  • Effect of natural mutations of SARS-CoV-2 on spike structure, conformation, and antigenicity. 
  • Exploiting the exploiter: a viral inhibitor stabilizes TAP for cryo-EM. 
  • Genetically encoded lipid-polypeptide hybrid biomaterials that exhibit temperature-triggered hierarchical self-assembly. 
  • Glutamate receptor desensitization is mediated by changes in quaternary structure of the ligand binding domain. 
  • HIV-1 envelope glycoprotein trimers display open quaternary conformation when bound to the gp41 membrane-proximal external-region-directed broadly neutralizing antibody Z13e1. 
  • Heat-dependent opening of TRPV1 in the presence of capsaicin. 
  • High-density binding to Plasmodium falciparum circumsporozoite protein repeats by inhibitory antibody elicited in mouse with human immunoglobulin repertoire. 
  • High-resolution structure determination using high-throughput electron cryo-tomography. 
  • Identification of IOMA-class neutralizing antibodies targeting the CD4-binding site on the HIV-1 envelope glycoprotein. 
  • Immunization expands B cells specific to HIV-1 V3 glycan in mice and macaques. 
  • Improved AlphaFold modeling with implicit experimental information. 
  • Improved chemistry restraints for crystallographic refinement by integrating the Amber force field into Phenix. 
  • Large conformational changes in a kinesin motor catalyzed by interaction with microtubules. 
  • Lateral density of receptor arrays in the membrane plane influences sensitivity of the E. coli chemotaxis response. 
  • Macromolecular structure determination using X-rays, neutrons and electrons: recent developments in Phenix. 
  • Mechanism by which T7 bacteriophage protein Gp1.2 inhibits Escherichia coli dGTPase. 
  • Membrane protein structure determination using cryo-electron tomography and 3D image averaging. 
  • Method for the structural analysis of Twinkle mitochondrial DNA helicase by cryo-EM. 
  • Methotrexate recognition by the human reduced folate carrier SLC19A1. 
  • Molecular architecture of native HIV-1 gp120 trimers. 
  • Molecular structure of a 9-MDa icosahedral pyruvate dehydrogenase subcomplex containing the E2 and E3 enzymes using cryoelectron microscopy. 
  • Naturally-occurring cholesterol analogues in lipid nanoparticles induce polymorphic shape and enhance intracellular delivery of mRNA. 
  • Neutralizing Antibody Responses Induced by HIV-1 Envelope Glycoprotein SOSIP Trimers Derived from Elite Neutralizers. 
  • New tools in MolProbity validation: CaBLAM for CryoEM backbone, UnDowser to rethink "waters," and NGL Viewer to recapture online 3D graphics. 
  • Polyclonal antibody responses to HIV Env immunogens resolved using cryoEM. 
  • Prefusion structure of trimeric HIV-1 envelope glycoprotein determined by cryo-electron microscopy. 
  • Protein secondary structure determination by constrained single-particle cryo-electron tomography. 
  • Quaternary contact in the initial interaction of CD4 with the HIV-1 envelope trimer. 
  • Recapitulation of HIV-1 Env-antibody coevolution in macaques leading to neutralization breadth. 
  • Receptor Switching in Newly Evolved Adeno-associated Viruses. 
  • Routine single particle CryoEM sample and grid characterization by tomography. 
  • Salt Responsive Morphologies of ssDNA-Based Triblock Polyelectrolytes in Semi-Dilute Regime: Effect of Volume Fractions and Polyelectrolyte Length. 
  • Sample preparation of the human TRPA1 ion channel for cryo-EM studies. 
  • Signaling at the endosome: cryo-EM structure of a GPCR-G protein-beta-arrestin megacomplex. 
  • Single-particle cryo-EM structure of a voltage-activated potassium channel in lipid nanodiscs. 
  • Spatial localization of the Ebola virus glycoprotein mucin-like domain determined by cryo-electron tomography. 
  • Spotiton: New features and applications. 
  • Structural Insights into Electrophile Irritant Sensing by the Human TRPA1 Channel. 
  • Structural and immunologic correlates of chemically stabilized HIV-1 envelope glycoproteins. 
  • Structural basis of NPR1 in activating plant immunity. 
  • Structural basis of cooling agent and lipid sensing by the cold-activated TRPM8 channel. 
  • Structural basis of glycan276-dependent recognition by HIV-1 broadly neutralizing antibodies. 
  • Structural impact of K63 ubiquitin on yeast translocating ribosomes under oxidative stress. 
  • Structural insight and characterization of human Twinkle helicase in mitochondrial disease. 
  • Structural mechanism of glutamate receptor activation and desensitization. 
  • Structural mechanism of trimeric HIV-1 envelope glycoprotein activation. 
  • Structurally Mapping Antigenic Epitopes of Adeno-associated Virus 9: Development of Antibody Escape Variants. 
  • Structure of an endosomal signaling GPCR-G protein-β-arrestin megacomplex. 
  • Structure of myosin filaments from relaxed Lethocerus flight muscle by cryo-EM at 6 Å resolution. 
  • Structure of the M2 muscarinic receptor-β-arrestin complex in a lipid nanodisc. 
  • Structure of the cold- and menthol-sensing ion channel TRPM8. 
  • Structure of β-galactosidase at 3.2-Å resolution obtained by cryo-electron microscopy. 
  • Structure, function and pharmacology of human itch GPCRs. 
  • Structure-based design of a quadrivalent fusion glycoprotein vaccine for human parainfluenza virus types 1-4. 
  • Structures of human Patched and its complex with native palmitoylated sonic hedgehog. 
  • Structures of the pleiotropic translational regulator Hfq and an Hfq-RNA complex: a bacterial Sm-like protein. 
  • Symmetry transitions during gating of the TRPV2 ion channel in lipid membranes. 
  • Tat-functionalized near-infrared emissive polymersomes for dendritic cell labeling. 
  • Tau induces formation of α-synuclein filaments with distinct molecular conformations. 
  • Temperature triggered self-assembly of polypeptides into multivalent spherical micelles. 
  • The Chimpanzee SIV Envelope Trimer: Structure and Deployment as an HIV Vaccine Template. 
  • The aquaporin sidedness revisited. 
  • The structure of helical lipoprotein lipase reveals an unexpected twist in lipase storage. 
  • Three-dimensional imaging of the highly bent architecture of Bdellovibrio bacteriovorus by using cryo-electron tomography. 
  • Three-dimensional structures of soluble CD4-bound states of trimeric simian immunodeficiency virus envelope glycoproteins determined by using cryo-electron tomography. 
  • Trimeric HIV-1 glycoprotein gp140 immunogens and native HIV-1 envelope glycoproteins display the same closed and open quaternary molecular architectures. 
  • Two Patched molecules engage distinct sites on Hedgehog yielding a signaling-competent complex. 
  • Untwisted α-Synuclein Filaments Formed in the Presence of Lipid Vesicles. 
  • Using Cryo-EM to Map Small Ligands on Dynamic Metabolic Enzymes: Studies with Glutamate Dehydrogenase. 
  • Vanilloid-dependent TRPV1 opening trajectory from cryoEM ensemble analysis. 
  • Viral epitope profiling of COVID-19 patients reveals cross-reactivity and correlates of severity. 
  • Visualization of alpha-helical features in a density map constructed using 9 molecular images of the 1.8 MDa icosahedral core of pyruvate dehydrogenase. 
  • Visualizing structural transitions of ligand-dependent gating of the TRPM2 channel.