Cryoelectron Microscopy
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Subject Areas on Research
- 2.2 Å resolution cryo-EM structure of β-galactosidase in complex with a cell-permeant inhibitor.
- 2.3 Å resolution cryo-EM structure of human p97 and mechanism of allosteric inhibition.
- A collaborative framework for 3D alignment and classification of heterogeneous subvolumes in cryo-electron tomography.
- A core-weighted fitting method for docking atomic structures into low-resolution maps: application to cryo-electron microscopy.
- A new way to see RNAs.
- An HIV-1 antibody from an elite neutralizer implicates the fusion peptide as a site of vulnerability.
- Assessment of detailed conformations suggests strategies for improving cryoEM models: Helix at lower resolution, ensembles, pre-refinement fixups, and validation at multi-residue length scale.
- Atomic Resolution Cryo-EM Structure of β-Galactosidase.
- Atomic resolution cryo-EM structure of a native-like CENP-A nucleosome aided by an antibody fragment.
- Automated image acquisition and processing using a new generation of 4K x 4K CCD cameras for cryo electron microscopic studies of macromolecular assemblies.
- Breaking Cryo-EM Resolution Barriers to Facilitate Drug Discovery.
- Caging metal ions with visible light-responsive nanopolymersomes.
- Classification of polyhedral shapes from individual anisotropically resolved cryo-electron tomography reconstructions.
- Computational separation of conformational heterogeneity using cryo-electron tomography and 3D sub-volume averaging.
- Conformational ensemble of the human TRPV3 ion channel.
- Constructing atomic structural models into cryo-EM densities using molecular dynamics - Pros and cons.
- Controlling the SARS-CoV-2 spike glycoprotein conformation.
- Cryo-EM Analysis of the Conformational Landscape of Human P-glycoprotein (ABCB1) During its Catalytic Cycle.
- Cryo-EM Structures Reveal Mechanism and Inhibition of DNA Targeting by a CRISPR-Cas Surveillance Complex.
- Cryo-EM Structures of the Magnesium Channel CorA Reveal Symmetry Break upon Gating.
- Cryo-EM reveals active site coordination within a multienzyme pre-rRNA processing complex.
- Cryo-EM structure of a mitochondrial calcium uniporter.
- Cryo-EM structure of human rhodopsin bound to an inhibitory G protein.
- Cryo-EM structure of the essential ribosome assembly AAA-ATPase Rix7.
- Cryo-electron Microscopy Structures of Chimeric Hemagglutinin Displayed on a Universal Influenza Vaccine Candidate.
- Cryo-electron microscopy structure of the TRPV2 ion channel.
- Cryo-electron microscopy structure of the lysosomal calcium-permeable channel TRPML3.
- Cryo-electron microscopy--a primer for the non-microscopist.
- Dengue virus and autophagy.
- Determination of molecular structures of HIV envelope glycoproteins using cryo-electron tomography and automated sub-tomogram averaging.
- Disc formation in cholesterol-free liposomes during phase transition.
- Discrete fracture patterns of virus shells reveal mechanical building blocks.
- Genetically encoded lipid-polypeptide hybrid biomaterials that exhibit temperature-triggered hierarchical self-assembly.
- Glutamate receptor desensitization is mediated by changes in quaternary structure of the ligand binding domain.
- HIV-1 envelope glycoprotein trimers display open quaternary conformation when bound to the gp41 membrane-proximal external-region-directed broadly neutralizing antibody Z13e1.
- Immunization expands B cells specific to HIV-1 V3 glycan in mice and macaques.
- Improved chemistry restraints for crystallographic refinement by integrating the Amber force field into Phenix.
- Large conformational changes in a kinesin motor catalyzed by interaction with microtubules.
- Lateral density of receptor arrays in the membrane plane influences sensitivity of the E. coli chemotaxis response.
- Macromolecular structure determination using X-rays, neutrons and electrons: recent developments in Phenix.
- Membrane protein structure determination using cryo-electron tomography and 3D image averaging.
- Molecular architecture of native HIV-1 gp120 trimers.
- Molecular structure of a 9-MDa icosahedral pyruvate dehydrogenase subcomplex containing the E2 and E3 enzymes using cryoelectron microscopy.
- Naturally-occurring cholesterol analogues in lipid nanoparticles induce polymorphic shape and enhance intracellular delivery of mRNA.
- New tools in MolProbity validation: CaBLAM for CryoEM backbone, UnDowser to rethink "waters," and NGL Viewer to recapture online 3D graphics.
- Prefusion structure of trimeric HIV-1 envelope glycoprotein determined by cryo-electron microscopy.
- Protein secondary structure determination by constrained single-particle cryo-electron tomography.
- Quaternary contact in the initial interaction of CD4 with the HIV-1 envelope trimer.
- Routine single particle CryoEM sample and grid characterization by tomography.
- Salt Responsive Morphologies of ssDNA-Based Triblock Polyelectrolytes in Semi-Dilute Regime: Effect of Volume Fractions and Polyelectrolyte Length.
- Single-particle cryo-EM structure of a voltage-activated potassium channel in lipid nanodiscs.
- Spatial localization of the Ebola virus glycoprotein mucin-like domain determined by cryo-electron tomography.
- Spotiton: New features and applications.
- Structural Insights into Electrophile Irritant Sensing by the Human TRPA1 Channel.
- Structural and immunologic correlates of chemically stabilized HIV-1 envelope glycoproteins.
- Structural basis of cooling agent and lipid sensing by the cold-activated TRPM8 channel.
- Structural impact of K63 ubiquitin on yeast translocating ribosomes under oxidative stress.
- Structural mechanism of glutamate receptor activation and desensitization.
- Structural mechanism of trimeric HIV-1 envelope glycoprotein activation.
- Structure of an endosomal signaling GPCR-G protein-β-arrestin megacomplex.
- Structure of the M2 muscarinic receptor-β-arrestin complex in a lipid nanodisc.
- Structure of the cold- and menthol-sensing ion channel TRPM8.
- Structure of β-galactosidase at 3.2-Å resolution obtained by cryo-electron microscopy.
- Structure-based design of a quadrivalent fusion glycoprotein vaccine for human parainfluenza virus types 1-4.
- Structures of human Patched and its complex with native palmitoylated sonic hedgehog.
- Structures of the pleiotropic translational regulator Hfq and an Hfq-RNA complex: a bacterial Sm-like protein.
- Symmetry transitions during gating of the TRPV2 ion channel in lipid membranes.
- Tat-functionalized near-infrared emissive polymersomes for dendritic cell labeling.
- Temperature triggered self-assembly of polypeptides into multivalent spherical micelles.
- The Chimpanzee SIV Envelope Trimer: Structure and Deployment as an HIV Vaccine Template.
- The aquaporin sidedness revisited.
- Three-dimensional imaging of the highly bent architecture of Bdellovibrio bacteriovorus by using cryo-electron tomography.
- Three-dimensional structures of soluble CD4-bound states of trimeric simian immunodeficiency virus envelope glycoproteins determined by using cryo-electron tomography.
- Trimeric HIV-1 glycoprotein gp140 immunogens and native HIV-1 envelope glycoproteins display the same closed and open quaternary molecular architectures.
- Two Patched molecules engage distinct sites on Hedgehog yielding a signaling-competent complex.
- Using Cryo-EM to Map Small Ligands on Dynamic Metabolic Enzymes: Studies with Glutamate Dehydrogenase.
- Visualization of alpha-helical features in a density map constructed using 9 molecular images of the 1.8 MDa icosahedral core of pyruvate dehydrogenase.
- Visualizing structural transitions of ligand-dependent gating of the TRPM2 channel.