Protein Structure, Quaternary

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  Subject Areas on Research

  • A complete algorithm to resolve ambiguity for intersubunit NOE assignment in structure determination of symmetric homo-oligomers. 
  • A geometric arrangement algorithm for structure determination of symmetric protein homo-oligomers from NOEs and RDCs. 
  • Activation of Slo1 BK channels by Mg2+ coordinated between the voltage sensor and RCK1 domains. 
  • Adenosine nucleotides and the regulation of GRP94-client protein interactions. 
  • Affinity maturation in an HIV broadly neutralizing B-cell lineage through reorientation of variable domains. 
  • African-derived genetic polymorphisms in TNFAIP3 mediate risk for autoimmunity. 
  • Blood traffic control. 
  • CD22 forms a quaternary complex with SHIP, Grb2, and Shc. A pathway for regulation of B lymphocyte antigen receptor-induced calcium flux. 
  • Computational de novo design and characterization of a four-helix bundle protein that selectively binds a nonbiological cofactor. 
  • Computational design and elaboration of a de novo heterotetrameric alpha-helical protein that selectively binds an emissive abiological (porphinato)zinc chromophore. 
  • Crystal structure of a PP2A B56-BubR1 complex and its implications for PP2A substrate recruitment and localization. 
  • Crystal structure of an efficacious gonococcal adherence inhibitor: an enolase from Lactobacillus gasseri. 
  • Crystal structure of molybdopterin synthase and its evolutionary relationship to ubiquitin activation. 
  • Crystal structure of the Haemophilus influenzae Hap adhesin reveals an intercellular oligomerization mechanism for bacterial aggregation. 
  • Crystal structures of the active and alloxanthine-inhibited forms of xanthine dehydrogenase from Rhodobacter capsulatus. 
  • Curved FtsZ protofilaments generate bending forces on liposome membranes. 
  • DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociation. 
  • Dependence of leucine-rich repeat kinase 2 (LRRK2) kinase activity on dimerization. 
  • Development of human protein reference database as an initial platform for approaching systems biology in humans. 
  • Differential loss of prolyl isomerase or chaperone activity of Ran-binding protein 2 (Ranbp2) unveils distinct physiological roles of its cyclophilin domain in proteostasis. 
  • Direct observation of multimer stabilization in the mechanical unfolding pathway of a protein undergoing oligomerization. 
  • Dynamics of Forced Nucleosome Unraveling and Role of Nonuniform Histone-DNA Interactions 
  • Electrostatic and lipid anchor contributions to the interaction of transducin with membranes: mechanistic implications for activation and translocation. 
  • Evidence for conservation of architecture and physical properties of Omp85-like proteins throughout evolution. 
  • Extracellular superoxide dismutase. 
  • Facile synthesis of site-specifically acetylated and methylated histone proteins: reagents for evaluation of the histone code hypothesis. 
  • Fatty acid synthase inhibition engages a novel caspase-2 regulatory mechanism to induce ovarian cancer cell death. 
  • Flexibility of the thrombin-activatable fibrinolysis inhibitor pro-domain enables productive binding of protein substrates. 
  • FtsZ filament capping by MciZ, a developmental regulator of bacterial division. 
  • Gamma-tubulin nucleation: template or protofilament? 
  • Glutamate receptor desensitization is mediated by changes in quaternary structure of the ligand binding domain. 
  • How an enzyme binds the C1 carrier tetrahydromethanopterin. Structure of the tetrahydromethanopterin-dependent formaldehyde-activating enzyme (Fae) from Methylobacterium extorquens AM1. 
  • Identification of novel quaternary domain interactions in the Hsp90 chaperone, GRP94. 
  • Implications for proteasome nuclear localization revealed by the structure of the nuclear proteasome tether protein Cut8. 
  • Induction of antibodies in rhesus macaques that recognize a fusion-intermediate conformation of HIV-1 gp41. 
  • Interactions of NO with hemoglobin: from microbes to man. 
  • Investigating extracellular in situ EGFR structure and conformational changes using FRET microscopy. 
  • Keeping G proteins at bay: a complex between G protein-coupled receptor kinase 2 and Gbetagamma. 
  • Mechanism of the Class I KDPG aldolase. 
  • Molecular architecture of native HIV-1 gp120 trimers. 
  • Molecular architectures of trimeric SIV and HIV-1 envelope glycoproteins on intact viruses: strain-dependent variation in quaternary structure. 
  • Monitoring of receptor dimerization using plasmonic coupling of gold nanoparticles. 
  • NAD+ modulates p53 DNA binding specificity and function. 
  • NMR structural inference of symmetric homo-oligomers. 
  • Nitric-oxide synthase forms N-NO-pterin and S-NO-cys: implications for activity, allostery, and regulation. 
  • PR48, a novel regulatory subunit of protein phosphatase 2A, interacts with Cdc6 and modulates DNA replication in human cells. 
  • Phage display screening for peptides that inhibit polyglutamine aggregation. 
  • Plant immunity requires conformational changes [corrected] of NPR1 via S-nitrosylation and thioredoxins. 
  • Polymorphic fibrillation of the destabilized fourth fasciclin-1 domain mutant A546T of the Transforming growth factor-β-induced protein (TGFBIp) occurs through multiple pathways with different oligomeric intermediates. 
  • Probing domain interactions in soluble guanylate cyclase. 
  • Protein secondary structure determination by constrained single-particle cryo-electron tomography. 
  • Quantification of the Impact of the HIV-1-Glycan Shield on Antibody Elicitation. 
  • Quaternary contact in the initial interaction of CD4 with the HIV-1 envelope trimer. 
  • Rapid double 8-nm steps by a kinesin mutant. 
  • Regulatory interactions between the Reg1-Glc7 protein phosphatase and the Snf1 protein kinase. 
  • Repetitive architecture of the Haemophilus influenzae Hia trimeric autotransporter. 
  • Ribosome-independent regulation of translocon composition and Sec61alpha conformation. 
  • Structural and immunologic correlates of chemically stabilized HIV-1 envelope glycoproteins. 
  • Structural basis for host recognition by the Haemophilus influenzae Hia autotransporter. 
  • Structural basis for the remarkable stability of Bacillus subtilis lipase (Lip A) at low pH. 
  • Structural basis of Rev1-mediated assembly of a quaternary vertebrate translesion polymerase complex consisting of Rev1, heterodimeric polymerase (Pol) ζ, and Pol κ. 
  • Structural characterization of the late competence protein ComFB from Bacillus subtilis. 
  • Structural determinants of autoproteolysis of the Haemophilus influenzae Hap autotransporter. 
  • Structural mechanism of ATP-induced polymerization of the partition factor ParF: implications for DNA segregation. 
  • Structural mechanism of trimeric HIV-1 envelope glycoprotein activation. 
  • Structure and accessibility of HA trimers on intact 2009 H1N1 pandemic influenza virus to stem region-specific neutralizing antibodies. 
  • Structure and immune recognition of trimeric pre-fusion HIV-1 Env. 
  • Structure determination of symmetric homo-oligomers by a complete search of symmetry configuration space, using NMR restraints and van der Waals packing. 
  • Structure of HIV-1 gp120 V1/V2 domain with broadly neutralizing antibody PG9. 
  • Structure of herpes simplex virus glycoprotein D bound to the human receptor nectin-1. 
  • Structure of mammalian protein geranylgeranyltransferase type-I. 
  • Structure of protein geranylgeranyltransferase-I from the human pathogen Candida albicans complexed with a lipid substrate. 
  • Structure of the outer membrane translocator domain of the Haemophilus influenzae Hia trimeric autotransporter. 
  • Structure of β-galactosidase at 3.2-Å resolution obtained by cryo-electron microscopy. 
  • Structures of partition protein ParA with nonspecific DNA and ParB effector reveal molecular insights into principles governing Walker-box DNA segregation. 
  • Structures of the Bacillus subtilis glutamine synthetase dodecamer reveal large intersubunit catalytic conformational changes linked to a unique feedback inhibition mechanism. 
  • Suppressing aberrant GluN3A expression rescues synaptic and behavioral impairments in Huntington's disease models. 
  • Surface loop dynamics in adeno-associated virus capsid assembly. 
  • Surfactant protein D decreases pollen-induced IgE-dependent mast cell degranulation. 
  • The 2.1 A structure of Aerococcus viridans L-lactate oxidase (LOX). 
  • The MerR-like protein BldC binds DNA direct repeats as cooperative multimers to regulate Streptomyces development. 
  • The MutSalpha-proliferating cell nuclear antigen interaction in human DNA mismatch repair. 
  • The Streptomyces master regulator BldD binds c-di-GMP sequentially to create a functional BldD2-(c-di-GMP)4 complex. 
  • The crystal structure of Escherichia coli MoeA and its relationship to the multifunctional protein gephyrin. 
  • The dual nature of human extracellular superoxide dismutase: one sequence and two structures. 
  • The effects of hypochlorous acid and neutrophil proteases on the structure and function of extracellular superoxide dismutase. 
  • The role of higher-order protein structure in supporting binding by heteroclitic monoclonal antibodies: the monoclonal antibody KIM185 to CD18 also binds C4-binding protein. 
  • The structure of a high fidelity DNA polymerase bound to a mismatched nucleotide reveals an "ajar" intermediate conformation in the nucleotide selection mechanism. 
  • The structure of irisin reveals a novel intersubunit β-sheet fibronectin type III (FNIII) dimer: implications for receptor activation. 
  • Three is better than one: pre-ligand receptor assembly in the regulation of TNF receptor signaling. 
  • Three-dimensional structures of soluble CD4-bound states of trimeric simian immunodeficiency virus envelope glycoproteins determined by using cryo-electron tomography. 
  • Total chemical synthesis and biophysical characterization of the minimal isoform of the KChIP2 potassium channel regulatory subunit. 
  • Translocator proteins in the two-partner secretion family have multiple domains. 
  • Trimeric HIV-1 glycoprotein gp140 immunogens and native HIV-1 envelope glycoproteins display the same closed and open quaternary molecular architectures. 
  • Using Cryo-EM to Map Small Ligands on Dynamic Metabolic Enzymes: Studies with Glutamate Dehydrogenase. 
  • Variations in IBD (ACAD8) in children with elevated C4-carnitine detected by tandem mass spectrometry newborn screening. 
  • Visualization of arrestin recruitment by a G-protein-coupled receptor. 
  • XMAP215 is a long thin molecule that does not increase microtubule stiffness. 
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  Keywords of People

  • Erickson, Harold Paul, James B. Duke Professor of Cell Biology, Biomedical Engineering
  • Luftig, Micah Alan, Associate Professor of Molecular Genetics and Microbiology, Molecular Genetics and Microbiology