Gene Silencing

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  Subject Areas on Research

  • 5' CpG island methylation of the FHIT gene is correlated with loss of gene expression in lung and breast cancer. 
  • A balancing act: RNA binding protein HuR/TTP axis in endometriosis patients. 
  • A computational analysis of antisense off-targets in prokaryotic organisms. 
  • A genome-wide RNAi screen reveals multiple regulators of caspase activation. 
  • A heterozygous IDH1R132H/WT mutation induces genome-wide alterations in DNA methylation. 
  • A novel Cbx1, PurB, and Sp3 complex mediates long-term silencing of tissue- and lineage-specific genes. 
  • APC promoter hypermethylation contributes to the loss of APC expression in colorectal cancers with allelic loss on 5q. 
  • Absence of Y chromosome in human placental site trophoblastic tumor. 
  • Activation of Akt/PDK signaling in macrophages upon binding of receptor-recognized forms of alpha2-macroglobulin to its cellular receptor: effect of silencing the CREB gene. 
  • Acyl carrier protein-specific 4'-phosphopantetheinyl transferase activates 10-formyltetrahydrofolate dehydrogenase. 
  • Adeno-associated virus-mediated ILK gene silencing in the rat NAc core. 
  • Alternative inclusion of fibroblast growth factor receptor 2 exon IIIc in Dunning prostate tumors reveals unexpected epithelial mesenchymal plasticity. 
  • An analysis of T cell intrinsic roles of E2A by conditional gene disruption in the thymus. 
  • An undecaprenyl phosphate-aminoarabinose flippase required for polymyxin resistance in Escherichia coli. 
  • Analysis of DNA methylation in a three-generation family reveals widespread genetic influence on epigenetic regulation. 
  • Angiotensin II-induced MMP-2 activity and MMP-14 and basigin protein expression are mediated via the angiotensin II receptor type 1-mitogen-activated protein kinase 1 pathway in retinal pigment epithelium: implications for age-related macular degeneration. 
  • Argonaute2 suppresses Drosophila fragile X expression preventing neurogenesis and oogenesis defects. 
  • Autophagic protein ULK1 regulates FOXM1 signalling in human hepatoma cells. 
  • BAHCC1 binds H3K27me3 via a conserved BAH module to mediate gene silencing and oncogenesis. 
  • BPIFB3 regulates autophagy and coxsackievirus B replication through a noncanonical pathway independent of the core initiation machinery. 
  • Binding of activated alpha2-macroglobulin to its cell surface receptor GRP78 in 1-LN prostate cancer cells regulates PAK-2-dependent activation of LIMK. 
  • C-terminally truncated derivatives of Escherichia coli Hfq are proficient in riboregulation. 
  • CD4(+) T cells contribute to the remodeling of the microenvironment required for sustained tumor regression upon oncogene inactivation. 
  • CLT1 targets bladder cancer through integrin α5β1 and CLIC3. 
  • Caspase-8 and caspase-3 small interfering RNA decreases ischemia/reperfusion injury to the liver in mice. 
  • Cell type-specific delivery of siRNAs with aptamer-siRNA chimeras. 
  • Centromere Silencing Mechanisms. 
  • Cleavage and polyadenylation specificity factor 1 (CPSF1) regulates alternative splicing of interleukin 7 receptor (IL7R) exon 6. 
  • Comparative RNAi screening reveals host factors involved in enterovirus infection of polarized endothelial monolayers. 
  • Comprehensive genomic profiling of pancreatic acinar cell carcinomas identifies recurrent RAF fusions and frequent inactivation of DNA repair genes. 
  • Computational Analysis of Targeting SARS-CoV-2, Viral Entry Proteins ACE2 and TMPRSS2, and Interferon Genes by Host MicroRNAs. 
  • Conditional gene silencing in mammalian cells mediated by a stress-inducible promoter. 
  • Convergence of Parkin, PINK1, and α-Synuclein on Stress-induced Mitochondrial Morphological Remodeling. 
  • Coordinate regulation of forskolin-induced cellular proliferation in macrophages by protein kinase A/cAMP-response element-binding protein (CREB) and Epac1-Rap1 signaling: effects of silencing CREB gene expression on Akt activation. 
  • Cyclophilin A and Ess1 interact with and regulate silencing by the Sin3-Rpd3 histone deacetylase. 
  • DNA adducts of decarbamoyl mitomycin C efficiently kill cells without wild-type p53 resulting from proteasome-mediated degradation of checkpoint protein 1. 
  • Decay of mutualistic potential in aphid endosymbionts through silencing of biosynthetic loci: Buchnera of Diuraphis. 
  • Derepression of BDNF transcription involves calcium-dependent phosphorylation of MeCP2. 
  • Dicentric chromosomes: unique models to study centromere function and inactivation. 
  • Dietary isothiocyanate-induced apoptosis via thiol modification of DNA topoisomerase IIα. 
  • Differential expression of the B'beta regulatory subunit of protein phosphatase 2A modulates tyrosine hydroxylase phosphorylation and catecholamine synthesis. 
  • Distinct gene expression profiles of acute myeloid/T-lymphoid leukemia with silenced CEBPA and mutations in NOTCH1. 
  • Drosophila PIWI associates with chromatin and interacts directly with HP1a. 
  • Dynamics of the epigenetic landscape during erythroid differentiation after GATA1 restoration. 
  • Endotoxin-stimulated nitric oxide production inhibits expression of cytochrome c oxidase in ANA-1 murine macrophages. 
  • Enforced expression of wild-type p53 curtails the transcription of the O(6)-methylguanine-DNA methyltransferase gene in human tumor cells and enhances their sensitivity to alkylating agents. 
  • Enteropathy-associated T cell lymphoma subtypes are characterized by loss of function of SETD2. 
  • Enzymatic properties of ALDH1L2, a mitochondrial 10-formyltetrahydrofolate dehydrogenase. 
  • Epigenetic Silencing of Recombinant Adeno-associated Virus Genomes by NP220 and the HUSH Complex. 
  • Epigenetic activation of unintegrated HIV-1 genomes by gut-associated short chain fatty acids and its implications for HIV infection. 
  • Epigenetic instability of cytokine and transcription factor gene loci underlies plasticity of the T helper 17 cell lineage. 
  • Epigenetic silencing of genes in human colon cancer. 
  • Epigenetic silencing of tumor suppressor Par-4 promotes chemoresistance in recurrent breast cancer. 
  • Epstein-Barr virus in gastric adenocarcinomas: association with ethnicity and CDKN2A promoter methylation. 
  • Estrogen-mediated epigenetic repression of large chromosomal regions through DNA looping. 
  • Exome sequencing reveals recurrent REV3L mutations in cisplatin-resistant squamous cell carcinoma of head and neck. 
  • Exosomal CCL2 from Tubular Epithelial Cells Is Critical for Albumin-Induced Tubulointerstitial Inflammation. 
  • Expression of ezrin correlates with malignant phenotype of lung cancer, and in vitro knockdown of ezrin reverses the aggressive biological behavior of lung cancer cells. 
  • FBW2 targets GCMa to the ubiquitin-proteasome degradation system. 
  • Fibronectin-integrin signaling is required for L-glutamine's protection against gut injury. 
  • Frequent ATRX, CIC, FUBP1 and IDH1 mutations refine the classification of malignant gliomas. 
  • Frequent inactivation of PTEN by promoter hypermethylation in microsatellite instability-high sporadic colorectal cancers. 
  • Functions of MutLalpha, replication protein A (RPA), and HMGB1 in 5'-directed mismatch repair. 
  • G Protein Coupled Receptor Kinase 3 Regulates Breast Cancer Migration, Invasion, and Metastasis. 
  • G protein-coupled receptor kinase 2 activates radixin, regulating membrane protrusion and motility in epithelial cells. 
  • G9a Promotes Breast Cancer Recurrence through Repression of a Pro-inflammatory Program. 
  • GGTase-I deficiency reduces tumor formation and improves survival in mice with K-RAS-induced lung cancer. 
  • Gain of glucose-independent growth upon metastasis of breast cancer cells to the brain. 
  • Gene expression and cell growth are modified by silencing SUMO2 and SUMO3 expression. 
  • Gene loss and parallel evolution contribute to species difference in flower color. 
  • Gene therapy beyond luxturna: a new horizon of the treatment for inherited retinal disease. 
  • Genetic inactivation of ANGPTL4 improves glucose homeostasis and is associated with reduced risk of diabetes. 
  • Genetic therapies for cardiovascular diseases. 
  • Genomewide view of gene silencing by small interfering RNAs. 
  • Genomic and Molecular Landscape of DNA Damage Repair Deficiency across The Cancer Genome Atlas. 
  • Germline AGO2 mutations impair RNA interference and human neurological development. 
  • Germline transcription from T-cell receptor Vbeta gene is uncoupled from allelic exclusion. 
  • Glutamine enhances heat shock protein 70 expression via increased hexosamine biosynthetic pathway activity. 
  • Glutathione transferase pi class 2 (GSTp2) protects against the cardiac deformities caused by exposure to PAHs but not PCB-126 in zebrafish embryos. 
  • HIN-1, a putative cytokine highly expressed in normal but not cancerous mammary epithelial cells. 
  • Hepatic FTO expression is increased in NASH and its silencing attenuates palmitic acid-induced lipotoxicity. 
  • High frequency of hypermethylation at the 14-3-3 sigma locus leads to gene silencing in breast cancer. 
  • Histone methyltransferase SETDB1 is required for prostate cancer cell proliferation, migration and invasion. 
  • Histone modifications and chromatin organization in prostate cancer. 
  • Human gamma-satellite DNA maintains open chromatin structure and protects a transgene from epigenetic silencing. 
  • Human origin recognition complex large subunit is degraded by ubiquitin-mediated proteolysis after initiation of DNA replication. 
  • Hypermethylation of 14-3-3 sigma (stratifin) is an early event in breast cancer. 
  • Identification of Proteins Bound to Dengue Viral RNA In Vivo Reveals New Host Proteins Important for Virus Replication. 
  • Impacts of silver nanoparticle coating on the nitrification potential of Nitrosomonas europaea. 
  • Imprinting evolution and the price of silence. 
  • Inactivation of the MAL gene in breast cancer is a common event that predicts benefit from adjuvant chemotherapy. 
  • Inactivation of the tumor suppressor genes causing the hereditary syndromes predisposing to head and neck cancer via promoter hypermethylation in sporadic head and neck cancers. 
  • Inhibition of Methicillin-resistant Staphylococcus aureus-induced cytokines mRNA production in human bone marrow derived mesenchymal stem cells by 1,25-dihydroxyvitamin D3. 
  • Integrated epigenomics identifies BMP4 as a modulator of cisplatin sensitivity in gastric cancer. 
  • Integrin-linked kinase is involved in cocaine sensitization by regulating PSD-95 and synapsin I expression and GluR1 Ser845 phosphorylation. 
  • Involvement of the checkpoint protein Mec1p in silencing of gene expression at telomeres in Saccharomyces cerevisiae. 
  • Junctophilin-2 expression silencing causes cardiocyte hypertrophy and abnormal intracellular calcium-handling. 
  • Loss of cyclin D2 expression in the majority of breast cancers is associated with promoter hypermethylation. 
  • Loss of expression of the p16 tumor suppressor gene is more frequent in advanced ovarian cancers lacking p53 mutations. 
  • Loss of heterozygosity of the putative prostate cancer susceptibility gene HPC2/ELAC2 is uncommon in sporadic and familial prostate cancer. 
  • Loss of type III transforming growth factor-beta receptor expression is due to methylation silencing of the transcription factor GATA3 in renal cell carcinoma. 
  • Loss-of-function genetic tools for animal models: cross-species and cross-platform differences. 
  • Loss-of-function genomic variants highlight potential therapeutic targets for cardiovascular disease. 
  • MSH6 inactivation and emergent temozolomide resistance in human glioblastomas. 
  • MeCP2 enforces Foxp3 expression to promote regulatory T cells' resilience to inflammation. 
  • Methylation of TFPI-2 gene is not the sole cause of its silencing. 
  • Methylation of the thyroid-stimulating hormone receptor gene in epithelial thyroid tumors: a marker of malignancy and a cause of gene silencing. 
  • Methylation subtypes and large-scale epigenetic alterations in gastric cancer. 
  • Microbial inactivation of Pseudomonas putida and Pichia pastoris using gene silencing. 
  • Mitochondrial transcription factor A induction by redox activation of nuclear respiratory factor 1. 
  • Modeling folate, one-carbon metabolism, and DNA methylation. 
  • Modulation of pancreatic cancer chemoresistance by inhibition of TAK1. 
  • Molecular characterization of the tumor-suppressive function of nischarin in breast cancer. 
  • Morphologically normal-appearing mammary epithelial cells obtained from high-risk women exhibit methylation silencing of INK4a/ARF. 
  • Mucor circinelloides: Growth, Maintenance, and Genetic Manipulation. 
  • Mutational analysis of the PTEN gene in human uterine sarcomas. 
  • Mutational inactivation of PTPRD in glioblastoma multiforme and malignant melanoma. 
  • NF1 inactivation in adult acute myelogenous leukemia. 
  • Negative regulation of CD4 gene expression by a HES-1-c-Myb complex. 
  • Neuron-specific Sumo1-3 knockdown in mice impairs episodic and fear memories. 
  • Nicotinamide Phosphoribosyltransferase Deficiency Potentiates the Antiproliferative Activity of Methotrexate through Enhanced Depletion of Intracellular ATP. 
  • Osteopontin silencing by small interfering RNA suppresses in vitro and in vivo CT26 murine colon adenocarcinoma metastasis. 
  • Overexpressed BAG3 is a potential therapeutic target in chronic lymphocytic leukemia. 
  • Overexpression of Arabidopsis MAP kinase kinase 7 leads to activation of plant basal and systemic acquired resistance. 
  • Paracrine modulation of cholangiocyte serotonin synthesis orchestrates biliary remodeling in adults. 
  • Periostin secreted by glioblastoma stem cells recruits M2 tumour-associated macrophages and promotes malignant growth. 
  • Pharmacological inhibition of a microRNA family in nonhuman primates by a seed-targeting 8-mer antimiR. 
  • Phospholipid-binding protein Cts1 controls septation and functions coordinately with calcineurin in Cryptococcus neoformans. 
  • Promoter hypermethylation profile of kidney cancer. 
  • Promoter methylation and silencing of the tissue factor pathway inhibitor-2 (TFPI-2), a gene encoding an inhibitor of matrix metalloproteinases in human glioma cells. 
  • Quantitative Proteomics Links the LRRC59 Interactome to mRNA Translation on the ER Membrane. 
  • RIP3 Regulates Autophagy and Promotes Coxsackievirus B3 Infection of Intestinal Epithelial Cells. 
  • RNA-guided transcriptional silencing in vivo with S. aureus CRISPR-Cas9 repressors. 
  • Rapid and reversible epigenome editing by endogenous chromatin regulators. 
  • Receptor-recognized α₂-macroglobulin binds to cell surface-associated GRP78 and activates mTORC1 and mTORC2 signaling in prostate cancer cells. 
  • Regulation of mitochondrial morphology by APC/CCdh1-mediated control of Drp1 stability. 
  • Regulatory crosstalk between lineage-survival oncogenes KLF5, GATA4 and GATA6 cooperatively promotes gastric cancer development. 
  • Ribosome stalling and SGS3 phase separation prime the epigenetic silencing of transposons. 
  • STIM1 signalling controls store-operated calcium entry required for development and contractile function in skeletal muscle. 
  • Sex-induced silencing operates during opposite-sex and unisexual reproduction in Cryptococcus neoformans. 
  • Silencing of cytosolic or mitochondrial isoforms of malic enzyme has no effect on glucose-stimulated insulin secretion from rodent islets. 
  • Single-cell CUT&Tag analysis of chromatin modifications in differentiation and tumor progression. 
  • Sir2 suppresses transcription-mediated displacement of Mcm2-7 replicative helicases at the ribosomal DNA repeats. 
  • Site-specific silencing of regulatory elements as a mechanism of X inactivation. 
  • Slow sodium channel inactivation and use-dependent block modulated by the same domain IV S6 residue. 
  • Somatic single hits inactivate the X-linked tumor suppressor FOXP3 in the prostate. 
  • Subtype-Specific Radiation Response and Therapeutic Effect of FAS Death Receptor Modulation in Human Breast Cancer. 
  • Subtype-specific 3D genome alteration in acute myeloid leukaemia. 
  • Sustained adrenergic signaling leads to increased metastasis in ovarian cancer via increased PGE2 synthesis. 
  • Syndecan-1 facilitates breast cancer metastasis to the brain. 
  • Synthetic microRNA designed to target glioma-associated antigen 1 transcription factor inhibits division and induces late apoptosis in pancreatic tumor cells. 
  • TGF-β-mediated silencing of genomic organizer SATB1 promotes Tfh cell differentiation and formation of intra-tumoral tertiary lymphoid structures. 
  • TP53 dysfunction in diffuse large B-cell lymphoma. 
  • The CO/HO system reverses inhibition of mitochondrial biogenesis and prevents murine doxorubicin cardiomyopathy. 
  • The PTEN tumor suppressor gene and its role in lymphoma pathogenesis. 
  • The Saccharomyces cerevisiae suppressor of choline sensitivity (SCS2) gene is a multicopy Suppressor of mec1 telomeric silencing defects. 
  • The granulin-epithelin precursor is a steroid-regulated growth factor in endometrial cancer. 
  • The role of MTJ-1 in cell surface translocation of GRP78, a receptor for alpha 2-macroglobulin-dependent signaling. 
  • The role of calcium-independent phospholipase A2γ in modulation of aqueous humor drainage and Ca2+ sensitization of trabecular meshwork contraction. 
  • The von Hippel-Lindau protein, vascular endothelial growth factor, and kidney cancer. 
  • Therapeutic rAAVrh10 Mediated SOD1 Silencing in Adult SOD1(G93A) Mice and Nonhuman Primates. 
  • Therapeutic targets for neuroblastomas. 
  • Transcriptional regulation of survivin by c-Myc in BCR/ABL-transformed cells: implications in anti-leukaemic strategy. 
  • Transcriptional repressor functions of Drosophila E2F1 and E2F2 cooperate to inhibit genomic DNA synthesis in ovarian follicle cells. 
  • Tumor Suppressors RB1 and CDKN2a Cooperatively Regulate Cell-Cycle Progression and Differentiation During Cardiomyocyte Development and Repair. 
  • Twist1 in podocytes ameliorates podocyte injury and proteinuria by limiting CCL2-dependent macrophage infiltration. 
  • U19/Eaf2 knockout causes lung adenocarcinoma, B-cell lymphoma, hepatocellular carcinoma and prostatic intraepithelial neoplasia. 
  • UBE2N Promotes Melanoma Growth via MEK/FRA1/SOX10 Signaling. 
  • Ubiquitylation of cyclin E requires the sequential function of SCF complexes containing distinct hCdc4 isoforms. 
  • Uncoupling of genomic and epigenetic signals in the maintenance and inheritance of heterochromatin domains in fission yeast. 
  • Unmasking a role for sex chromosomes in gene silencing. 
  • Upregulation of mTORC2 activation by the selective agonist of EPAC, 8-CPT-2Me-cAMP, in prostate cancer cells: assembly of a multiprotein signaling complex. 
  • c-Jun Contributes to Transcriptional Control of GNA12 Expression in Prostate Cancer Cells. 
  • miR-27b shapes the presynaptic transcriptome and influences neurotransmission by silencing the polycomb group protein Bmi1. 
  • p38 Mitogen-activated protein kinase plays a stimulatory role in hepatic gluconeogenesis. 
  • β-Secretase (BACE1) inhibition causes retinal pathology by vascular dysregulation and accumulation of age pigment. 
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  Keywords of People

  • Berchuck, Andrew, James M. Ingram Distinguished Professor of Gynecologic Oncology, Obstetrics and Gynecology, Gynecologic Oncology
  • Choi, Steven Sok, Associate Professor of Medicine, Medicine, Gastroenterology
  • Guan, Ziqiang, Research Professor in Biochemistry, Biochemistry
  • Landstrom, Andrew Paul, Associate Professor of Pediatrics, Cell Biology
  • McLendon, Roger Edwin, Professor of Pathology, Pathology
  • Starmer, Charles Franklin, Professor Emeritus of Computer Science, Computer Science
  • Sullivan, Beth Ann, Professor of Molecular Genetics and Microbiology, Duke Science & Society