Fluorescence Resonance Energy Transfer
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Subject Areas on Research
- A DNA nanotransport device powered by polymerase phi29.
- A two-state DNA lattice switched by DNA nanoactuator.
- Allosteric Inhibition of the Protein-Protein Interaction between the Leukemia-Associated Proteins Runx1 and CBFβ
- An experimental study of GFP-based FRET, with application to intrinsically unstructured proteins.
- Analysis of the eukaryotic topoisomerase II DNA gate: a single-molecule FRET and structural perspective.
- Assembly dynamics of Mycobacterium tuberculosis FtsZ.
- Assembly of the bacteriophage T4 primosome: single-molecule and ensemble studies.
- Autocrine BDNF-TrkB signalling within a single dendritic spine.
- Cation-Activated Avidity for Rapid Reconfiguration of DNA Nanodevices.
- Cell division requires a direct link between microtubule-bound RacGAP and Anillin in the contractile ring.
- Combining QD-FRET and microfluidics to monitor DNA nanocomplex self-assembly in real-time.
- Construction, imaging, and analysis of FRET-based tension sensors in living cells.
- Design of an Escherichia coli expressed HIV-1 gp120 fragment immunogen that binds to b12 and induces broad and potent neutralizing antibodies.
- Development of a high-throughput screening assay for inhibitors of small ubiquitin-like modifier proteases.
- Differential and simultaneous adenosine di- and triphosphate binding by MutS.
- Differential specificities and simultaneous occupancy of human MutSalpha nucleotide binding sites.
- Dissociation kinetics of the GroEL-gp31 chaperonin complex studied with Förster resonance energy transfer.
- Distinct conformational changes in beta-arrestin report biased agonism at seven-transmembrane receptors.
- Enhanced Archaerhodopsin Fluorescent Protein Voltage Indicators.
- Evaluating the intracellular stability and unpacking of DNA nanocomplexes by quantum dots-FRET.
- Features of programmed cell death in intact Xenopus oocytes and early embryos revealed by near-infrared fluorescence and real-time monitoring.
- FtsZ filament dynamics at steady state: subunit exchange with and without nucleotide hydrolysis.
- G-protein-coupled receptor kinase specificity for beta-arrestin recruitment to the beta2-adrenergic receptor revealed by fluorescence resonance energy transfer.
- Gender differences in cholesterol nucleation in native bile: estrogen is a potential contributory factor.
- Heteromeric assembly of acid-sensitive ion channel and epithelial sodium channel subunits.
- Hfq structure, function and ligand binding.
- High-speed recording of neural spikes in awake mice and flies with a fluorescent voltage sensor.
- How the kinetochore couples microtubule force and centromere stretch to move chromosomes.
- Identification and structure-activity relationship of phenolic acyl hydrazones as selective agonists for the estrogen-related orphan nuclear receptors ERRbeta and ERRgamma.
- Identification of calcium-modulating cyclophilin ligand (CAML) as transducer of angiotensin II-mediated nuclear factor of activated T cells (NFAT) activation.
- Imaging cyclic AMP changes in pancreatic islets of transgenic reporter mice.
- Imaging neural spiking in brain tissue using FRET-opsin protein voltage sensors.
- Imaging spatiotemporal dynamics of neuronal signaling using fluorescence resonance energy transfer and fluorescence lifetime imaging microscopy.
- Improving FRET dynamic range with bright green and red fluorescent proteins.
- Improving Quality, Reproducibility, and Usability of FRET-Based Tension Sensors.
- Interaction between the T4 helicase loading protein (gp59) and the DNA polymerase (gp43): unlocking of the gp59-gp43-DNA complex to initiate assembly of a fully functional replisome.
- Interaction between the T4 helicase-loading protein (gp59) and the DNA polymerase (gp43): a locking mechanism to delay replication during replisome assembly.
- Investigating extracellular in situ EGFR structure and conformational changes using FRET microscopy.
- L-selectin shedding is activated specifically within transmigrating pseudopods of monocytes to regulate cell polarity in vitro.
- Lateral density of receptor arrays in the membrane plane influences sensitivity of the E. coli chemotaxis response.
- Low temperature and chemical rescue affect molecular proximity of DeltaF508-cystic fibrosis transmembrane conductance regulator (CFTR) and epithelial sodium channel (ENaC).
- Microfluidic preparation of polymer-nucleic acid nanocomplexes improves nonviral gene transfer.
- Molecular and biophysical analysis of apoptosis using a combined quantitative phase imaging and fluorescence resonance energy transfer microscope.
- Molecular proximity of cystic fibrosis transmembrane conductance regulator and epithelial sodium channel assessed by fluorescence resonance energy transfer.
- Monitoring the topoisomerase II DNA gate conformational change with fluorescence resonance energy transfer.
- Monitoring β-secretase activity in living cells with a membrane-anchored FRET probe.
- Most mutations that cause spinocerebellar ataxia autosomal recessive type 16 (SCAR16) destabilize the protein quality-control E3 ligase CHIP.
- Multiplexed 3D FRET imaging in deep tissue of live embryos.
- Near-simultaneous and real-time detection of multiple analytes in affinity microcolumns.
- On the intractability of estrogen-related receptor alpha as a target for activation by small molecules.
- Optogenetic probes.
- Pharmacological characterization of membrane-expressed human trace amine-associated receptor 1 (TAAR1) by a bioluminescence resonance energy transfer cAMP biosensor.
- Protonophore- and pH-insensitive glucose and sucrose accumulation detected by FRET nanosensors in Arabidopsis root tips.
- Quantification of microRNAs, splicing isoforms, and homologous mRNAs with the invader assay.
- Quantitative comparison of intracellular unpacking kinetics of polyplexes by a model constructed from quantum dot-FRET.
- Quantum dot-based theranostics.
- Rapid in vitro assembly dynamics and subunit turnover of FtsZ demonstrated by fluorescence resonance energy transfer.
- Rational design of a calcium-binding protein.
- Regulation of Rac1 translocation and activation by membrane domains and their boundaries.
- Resonating to the music of ubiquitination.
- Robust approaches to quantitative ratiometric FRET imaging of CFP/YFP fluorophores under confocal microscopy.
- Single transcriptional and translational preQ1 riboswitches adopt similar pre-folded ensembles that follow distinct folding pathways into the same ligand-bound structure.
- Single-molecule FRET reveals multiscale chromatin dynamics modulated by HP1α.
- Single-molecule measurements of the opening and closing of the DNA gate by eukaryotic topoisomerase II.
- Software for lattice light-sheet imaging of FRET biosensors, illustrated with a new Rap1 biosensor.
- Stable docking of neutralizing human immunodeficiency virus type 1 gp41 membrane-proximal external region monoclonal antibodies 2F5 and 4E10 is dependent on the membrane immersion depth of their epitope regions.
- Stretching fibronectin.
- Studying 3D subdomains of proteins at the nanometer scale using fluorescence spectroscopy.
- Studying signal transduction in single dendritic spines.
- TR-FRET-based duplex immunoassay reveals an inverse correlation of soluble and aggregated mutant huntingtin in huntington's disease.
- The alpha1a-adrenergic receptor occupies membrane rafts with its G protein effectors but internalizes via clathrin-coated pits.
- The convergence of quantum-dot-mediated fluorescence resonance energy transfer and microfluidics for monitoring DNA polyplex self-assembly in real time.
- The evolving capabilities of rhodopsin-based genetically encoded voltage indicators.
- The nicotinic α6 subunit gene determines variability in chronic pain sensitivity via cross-inhibition of P2X2/3 receptors.
- Thrombin-cleaved COOH(-) terminal osteopontin peptide binds with cyclophilin C to CD147 in murine breast cancer cells.
- Transient opening of fibronectin type III (FNIII) domains: the interaction of the third FNIII domain of FN with anastellin.
- Tunable molecular tension sensors reveal extension-based control of vinculin loading.
- Two-state displacement by the kinesin-14 Ncd stalk.
- Understanding nonviral nucleic acid delivery with quantum dot-FRET nanosensors.
- Uptake and intracellular fate of multifunctional nanoparticles: a comparison between lipoplexes and polyplexes via quantum dot mediated Förster resonance energy transfer.
- beta-arrestin-biased agonism at the beta2-adrenergic receptor.
- beta2-adrenergic receptor signaling and desensitization elucidated by quantitative modeling of real time cAMP dynamics.
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Keywords of People
- Bejsovec, Amy, Associate Professor of Biology, Biology
- Erickson, Harold Paul, James B. Duke Distinguished Professor of Cell Biology, Cell Biology