Fluorescence Resonance Energy Transfer
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Subject Areas on Research
- A DNA nanotransport device powered by polymerase phi29.
- A high-speed, bright, red fluorescent voltage sensor to detect neural activity.
- A near-infrared genetically encoded calcium indicator for in vivo imaging.
- A two-state DNA lattice switched by DNA nanoactuator.
- An experimental study of GFP-based FRET, with application to intrinsically unstructured proteins.
- Assembly dynamics of Mycobacterium tuberculosis FtsZ.
- Autocrine BDNF-TrkB signalling within a single dendritic spine.
- Cation-Activated Avidity for Rapid Reconfiguration of DNA Nanodevices.
- Cell division requires a direct link between microtubule-bound RacGAP and Anillin in the contractile ring.
- Construction, imaging, and analysis of FRET-based tension sensors in living cells.
- Design of an Escherichia coli expressed HIV-1 gp120 fragment immunogen that binds to b12 and induces broad and potent neutralizing antibodies.
- Development of a high-throughput screening assay for inhibitors of small ubiquitin-like modifier proteases.
- Differential and simultaneous adenosine di- and triphosphate binding by MutS.
- Differential specificities and simultaneous occupancy of human MutSalpha nucleotide binding sites.
- Dissociation kinetics of the GroEL-gp31 chaperonin complex studied with Förster resonance energy transfer.
- Distinct conformational changes in beta-arrestin report biased agonism at seven-transmembrane receptors.
- Domain Organization in the 54-kDa Subunit of the Chloroplast Signal Recognition Particle.
- Engineering rhodopsins' activation spectra using a FRET-based approach.
- FRET efficiency measurement in a molecular tension probe with a low-cost frequency-domain fluorescence lifetime imaging microscope.
- Features of programmed cell death in intact Xenopus oocytes and early embryos revealed by near-infrared fluorescence and real-time monitoring.
- Flow cytometric analysis of fluorescence resonance energy transfer: a tool for high-throughput screening of molecular interactions in living cells.
- Fretting about FRET: correlation between kappa and R.
- FtsZ filament dynamics at steady state: subunit exchange with and without nucleotide hydrolysis.
- G-protein-coupled receptor kinase specificity for beta-arrestin recruitment to the beta2-adrenergic receptor revealed by fluorescence resonance energy transfer.
- Gender differences in cholesterol nucleation in native bile: estrogen is a potential contributory factor.
- Hfq structure, function and ligand binding.
- How the kinetochore couples microtubule force and centromere stretch to move chromosomes.
- Identification and structure-activity relationship of phenolic acyl hydrazones as selective agonists for the estrogen-related orphan nuclear receptors ERRbeta and ERRgamma.
- Identification of calcium-modulating cyclophilin ligand (CAML) as transducer of angiotensin II-mediated nuclear factor of activated T cells (NFAT) activation.
- Imaging spatiotemporal dynamics of neuronal signaling using fluorescence resonance energy transfer and fluorescence lifetime imaging microscopy.
- Improving Quality, Reproducibility, and Usability of FRET-Based Tension Sensors.
- L-selectin shedding is activated specifically within transmigrating pseudopods of monocytes to regulate cell polarity in vitro.
- Lateral density of receptor arrays in the membrane plane influences sensitivity of the E. coli chemotaxis response.
- Mechanism and function of synaptotagmin-mediated membrane apposition.
- Molecular and biophysical analysis of apoptosis using a combined quantitative phase imaging and fluorescence resonance energy transfer microscope.
- Monitoring molecular interactions in living cells using flow cytometric analysis of fluorescence resonance energy transfer.
- Monitoring β-secretase activity in living cells with a membrane-anchored FRET probe.
- Most mutations that cause spinocerebellar ataxia autosomal recessive type 16 (SCAR16) destabilize the protein quality-control E3 ligase CHIP.
- Multiplexed GTPase and GEF biosensor imaging enables network connectivity analysis.
- On the intractability of estrogen-related receptor alpha as a target for activation by small molecules.
- Optogenetic probes.
- Pharmacological characterization of membrane-expressed human trace amine-associated receptor 1 (TAAR1) by a bioluminescence resonance energy transfer cAMP biosensor.
- Protonophore- and pH-insensitive glucose and sucrose accumulation detected by FRET nanosensors in Arabidopsis root tips.
- Rapid in vitro assembly dynamics and subunit turnover of FtsZ demonstrated by fluorescence resonance energy transfer.
- Rational design of a calcium-binding protein.
- Recognition Patterns of the C1/C2 Epitopes Involved in Fc-Mediated Response in HIV-1 Natural Infection and the RV114 Vaccine Trial.
- Regulation of Rac1 translocation and activation by membrane domains and their boundaries.
- Resonating to the music of ubiquitination.
- Robust approaches to quantitative ratiometric FRET imaging of CFP/YFP fluorophores under confocal microscopy.
- Simplified Instrument Calibration for Wide-Field Fluorescence Resonance Energy Transfer (FRET) Measured by the Sensitized Emission Method.
- Single transcriptional and translational preQ1 riboswitches adopt similar pre-folded ensembles that follow distinct folding pathways into the same ligand-bound structure.
- Single-molecule FRET reveals multiscale chromatin dynamics modulated by HP1α.
- Software for lattice light-sheet imaging of FRET biosensors, illustrated with a new Rap1 biosensor.
- Stable docking of neutralizing human immunodeficiency virus type 1 gp41 membrane-proximal external region monoclonal antibodies 2F5 and 4E10 is dependent on the membrane immersion depth of their epitope regions.
- Stretching fibronectin.
- Studying 3D subdomains of proteins at the nanometer scale using fluorescence spectroscopy.
- Studying signal transduction in single dendritic spines.
- The nicotinic α6 subunit gene determines variability in chronic pain sensitivity via cross-inhibition of P2X2/3 receptors.
- Thrombin-cleaved COOH(-) terminal osteopontin peptide binds with cyclophilin C to CD147 in murine breast cancer cells.
- Transient opening of fibronectin type III (FNIII) domains: the interaction of the third FNIII domain of FN with anastellin.
- Tunable molecular tension sensors reveal extension-based control of vinculin loading.
- Two-state displacement by the kinesin-14 Ncd stalk.
- Unusual mode of dimerization of retinitis pigmentosa-associated F220C rhodopsin.
- beta-arrestin-biased agonism at the beta2-adrenergic receptor.
- beta2-adrenergic receptor signaling and desensitization elucidated by quantitative modeling of real time cAMP dynamics.
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Keywords of People
- Bejsovec, Amy, Associate Professor of Biology, Biology
- Erickson, Harold Paul, Professor Emeritus of Cell Biology, Cell Biology