Fluorescence Resonance Energy Transfer
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Subject Areas on Research
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A DNA nanotransport device powered by polymerase phi29.
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A high-speed, bright, red fluorescent voltage sensor to detect neural activity.
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A two-state DNA lattice switched by DNA nanoactuator.
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An experimental study of GFP-based FRET, with application to intrinsically unstructured proteins.
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Assembly dynamics of Mycobacterium tuberculosis FtsZ.
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Autocrine BDNF-TrkB signalling within a single dendritic spine.
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Cation-Activated Avidity for Rapid Reconfiguration of DNA Nanodevices.
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Cell division requires a direct link between microtubule-bound RacGAP and Anillin in the contractile ring.
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Construction, imaging, and analysis of FRET-based tension sensors in living cells.
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Design of an Escherichia coli expressed HIV-1 gp120 fragment immunogen that binds to b12 and induces broad and potent neutralizing antibodies.
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Development of a high-throughput screening assay for inhibitors of small ubiquitin-like modifier proteases.
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Differential and simultaneous adenosine di- and triphosphate binding by MutS.
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Differential specificities and simultaneous occupancy of human MutSalpha nucleotide binding sites.
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Dissociation kinetics of the GroEL-gp31 chaperonin complex studied with Förster resonance energy transfer.
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Distinct conformational changes in beta-arrestin report biased agonism at seven-transmembrane receptors.
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Enhanced Archaerhodopsin Fluorescent Protein Voltage Indicators.
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FRET efficiency measurement in a molecular tension probe with a low-cost frequency-domain fluorescence lifetime imaging microscope.
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Features of programmed cell death in intact Xenopus oocytes and early embryos revealed by near-infrared fluorescence and real-time monitoring.
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FtsZ filament dynamics at steady state: subunit exchange with and without nucleotide hydrolysis.
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G-protein-coupled receptor kinase specificity for beta-arrestin recruitment to the beta2-adrenergic receptor revealed by fluorescence resonance energy transfer.
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Gender differences in cholesterol nucleation in native bile: estrogen is a potential contributory factor.
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Hfq structure, function and ligand binding.
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High-speed recording of neural spikes in awake mice and flies with a fluorescent voltage sensor.
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How the kinetochore couples microtubule force and centromere stretch to move chromosomes.
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Identification and structure-activity relationship of phenolic acyl hydrazones as selective agonists for the estrogen-related orphan nuclear receptors ERRbeta and ERRgamma.
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Identification of calcium-modulating cyclophilin ligand (CAML) as transducer of angiotensin II-mediated nuclear factor of activated T cells (NFAT) activation.
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Imaging neural spiking in brain tissue using FRET-opsin protein voltage sensors.
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Imaging spatiotemporal dynamics of neuronal signaling using fluorescence resonance energy transfer and fluorescence lifetime imaging microscopy.
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Improving FRET dynamic range with bright green and red fluorescent proteins.
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Improving Quality, Reproducibility, and Usability of FRET-Based Tension Sensors.
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L-selectin shedding is activated specifically within transmigrating pseudopods of monocytes to regulate cell polarity in vitro.
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Lateral density of receptor arrays in the membrane plane influences sensitivity of the E. coli chemotaxis response.
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Molecular and biophysical analysis of apoptosis using a combined quantitative phase imaging and fluorescence resonance energy transfer microscope.
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Monitoring β-secretase activity in living cells with a membrane-anchored FRET probe.
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Most mutations that cause spinocerebellar ataxia autosomal recessive type 16 (SCAR16) destabilize the protein quality-control E3 ligase CHIP.
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Multiplexed GTPase and GEF biosensor imaging enables network connectivity analysis.
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On the intractability of estrogen-related receptor alpha as a target for activation by small molecules.
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Optogenetic probes.
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Pharmacological characterization of membrane-expressed human trace amine-associated receptor 1 (TAAR1) by a bioluminescence resonance energy transfer cAMP biosensor.
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Protonophore- and pH-insensitive glucose and sucrose accumulation detected by FRET nanosensors in Arabidopsis root tips.
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Quantification of microRNAs, splicing isoforms, and homologous mRNAs with the invader assay.
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Rapid in vitro assembly dynamics and subunit turnover of FtsZ demonstrated by fluorescence resonance energy transfer.
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Rational design of a calcium-binding protein.
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Regulation of Rac1 translocation and activation by membrane domains and their boundaries.
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Resonating to the music of ubiquitination.
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Robust approaches to quantitative ratiometric FRET imaging of CFP/YFP fluorophores under confocal microscopy.
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Single transcriptional and translational preQ1 riboswitches adopt similar pre-folded ensembles that follow distinct folding pathways into the same ligand-bound structure.
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Single-molecule FRET reveals multiscale chromatin dynamics modulated by HP1α.
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Software for lattice light-sheet imaging of FRET biosensors, illustrated with a new Rap1 biosensor.
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Stable docking of neutralizing human immunodeficiency virus type 1 gp41 membrane-proximal external region monoclonal antibodies 2F5 and 4E10 is dependent on the membrane immersion depth of their epitope regions.
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Stretching fibronectin.
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Studying 3D subdomains of proteins at the nanometer scale using fluorescence spectroscopy.
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Studying signal transduction in single dendritic spines.
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The evolving capabilities of rhodopsin-based genetically encoded voltage indicators.
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The nicotinic α6 subunit gene determines variability in chronic pain sensitivity via cross-inhibition of P2X2/3 receptors.
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Thrombin-cleaved COOH(-) terminal osteopontin peptide binds with cyclophilin C to CD147 in murine breast cancer cells.
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Transient opening of fibronectin type III (FNIII) domains: the interaction of the third FNIII domain of FN with anastellin.
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Tunable molecular tension sensors reveal extension-based control of vinculin loading.
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Two-state displacement by the kinesin-14 Ncd stalk.
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beta-arrestin-biased agonism at the beta2-adrenergic receptor.
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beta2-adrenergic receptor signaling and desensitization elucidated by quantitative modeling of real time cAMP dynamics.
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Keywords of People