Culture Techniques

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  Subject Areas on Research

  • A comparison of the leukocyte agglutination and mixed agglutination techniques for detecting human tissue isoantigens. 
  • A demonstration in vitro of luteolytic activity in pig uterine flushings. 
  • A genetic approach for studying the physiology of the type 1A (AT1A) angiotensin receptor. 
  • A perfusion chamber for the study of CNS physiology and pharmacology in vitro. 
  • A reliable method for isolation of viable porcine islet cells. 
  • A review of three-dimensional in vitro tissue models for drug discovery and transport studies. 
  • Age-dependent inhibition of long-term potentiation by ethanol in immature versus mature hippocampus. 
  • Age-specific demographic profiles of longevity mutants in Caenorhabditis elegans show segmental effects. 
  • Altered cocaine potency in the nucleus accumbens following 7-day withdrawal from intermittent but not continuous treatment: voltammetric assessment of dopamine uptake in the rat. 
  • Axon terminal hyperexcitability associated with epileptogenesis in vitro. I. Origin of ectopic spikes. 
  • Axon terminal hyperexcitability associated with epileptogenesis in vitro. II. Pharmacological regulation by NMDA and GABAA receptors. 
  • BAI strain A avian (myeloblastosis) leukosis virus from myeloblast tissue culture. 
  • Bacterial meningitis in infants. 
  • Blocking of autologous and homologous leukocyte responses by human alloimmune plasmas: a possible in vitro correlate of enhancement. 
  • Bone morphogenetic proteins (BMPs) as regulators of dorsal forebrain development. 
  • Bovine retinal explants cultured in collagen gels. A model system for the study of proliferative retinopathy. 
  • Calcium accumulation by isolated sarcoplasmic reticulum of skeletal muscle during development in tissue culture. 
  • Cardiac muscle tissue engineering: toward an in vitro model for electrophysiological studies. 
  • Catecholaminergic cell lines from the brain and adrenal glands of tyrosine hydroxylase-SV40 T antigen transgenic mice. 
  • Characterization of keratinocyte plasminogen activator inhibitors and demonstration of the prevention of pemphigus IgG-induced acantholysis by a purified plasminogen activator inhibitor. 
  • Chlorpyrifos elicits mitotic abnormalities and apoptosis in neuroepithelium of cultured rat embryos. 
  • Chondrogenic potential of adipose tissue-derived stromal cells in vitro and in vivo. 
  • Chordin and noggin promote organizing centers of forebrain development in the mouse. 
  • Chorioallantoic membrane grafting of the embryonic murine kidney. An improved in vitro technique for studying kidney morphogenesis. 
  • Cloning efficiency of cultured human tumor cell lines measured with the use of a Coulter particle counter. 
  • Cloning of mitogen- and antigen-reactive B lymphocytes on filter paper disks: phenotypic and genotypic analysis of B cell colonies. 
  • Collagenase and connective tissue metabolism in epidermolysis bullosa. 
  • Collagenase in bone of man. 
  • Collagenase production by human dermis in vitro. 
  • Collision of excitation waves in the dog Purkinje system. Extracellular identification. 
  • Comparing Standard Versus Extended Culture Duration in Acute Hip and Knee Periprosthetic Joint Infection. 
  • Continuous human glioma-derived cell lines UC-11MG and UC-302MG. Morphologic, immunocytochemical and chromosomal characterization. 
  • Control of proteoglycan synthesis. Studies on the activation of synthesis observed during culture of articular cartilages. 
  • Culture of hepatocytes on fructose-modified chitosan scaffolds. 
  • Cystine-glutamate transport interactions in rat renal cortical tubules, brushborder vesicles, and cultured renal tubule cells. 
  • Density and distribution of hippocampal neurotransmitter receptors in autism: an autoradiographic study. 
  • Depressed monocyte chemotaxis during acute influenza infection. 
  • Depression of human monocyte chemotaxis by herpes simplex and influenza viruses. 
  • Diarrhoea associated with heat-stable enterotoxin-producing strains of Escherichia coli. 
  • Differential expression of GABA and glutamate-receptor subunits and enzymes involved in GABA metabolism between electrophysiologically identified hippocampal CA1 pyramidal cells and interneurons. 
  • Differential sensitivity of NMDA receptor-mediated synaptic potentials to ethanol in immature versus mature hippocampus. 
  • Direct antidiabetic effect of leptin through triglyceride depletion of tissues. 
  • Effect of a NOS inhibitor, L-NMMA, on the contractile function of reperfused skeletal muscle. 
  • Effect of basic fibroblast growth factor on angiogenesis and growth of isografted bone: quantitative in vitro-in vivo analysis in mice. 
  • Effects of phytanic acid on the vitamin E status, lipid composition and physical properties of retinal cell membranes: implications for adult Refsum disease. 
  • Effects of tissue constraint on shock wave-induced bubble expansion in vivo. 
  • Endothelial cells adhere to the RGD domain and the fibrinogen-like terminal knob of tenascin. 
  • Enzymatic susceptibility and spontaneous release of human melanoma tumor-associated antigens. 
  • Establishment of the DU.528 human lymphohemopoietic stem cell line. 
  • Ethanol inhibition of AMPA and kainate receptor-mediated depolarizations of hippocampal area CA1. 
  • Evidence that a precursor glycoprotein is cleaved to yield the major glycoprotein of avian tumor virus. 
  • Evolutionary modification of cell lineage in the direct-developing sea urchin Heliocidaris erythrogramma. 
  • Expression in normal human tissues of five nucleotide excision repair genes measured simultaneously by multiplex reverse transcription-polymerase chain reaction. 
  • Fluorescent labeling of dissociated fetal cells for tissue culture. 
  • Fumonisins disrupt sphingolipid metabolism, folate transport, and neural tube development in embryo culture and in vivo: a potential risk factor for human neural tube defects among populations consuming fumonisin-contaminated maize. 
  • GABAB autoreceptors mediate activity-dependent disinhibition and enhance signal transmission in the dentate gyrus. 
  • GABAB receptors mediate disinhibition and facilitate long-term potentiation in the dentate gyrus. 
  • Genetic studies in Alzheimer's disease with an NACP/alpha-synuclein polymorphism. 
  • HL-A antigenic loss in malignant transformation. 
  • Heating or freezing bone. Effects on angiogenesis induction and growth potential in mice. 
  • High-resolution cross-sectional imaging of the gastrointestinal tract using optical coherence tomography: preliminary results. 
  • Human fetal liver hematopoietic cell expansion with a novel bioreactor system. 
  • Hyperglycemia induces insulin resistance on angiotensinogen gene expression in diabetic rat kidney proximal tubular cells. 
  • Implementing a urinary tract infection clinical practice guideline in an ambulatory urgent care practice. 
  • In vitro chemotherapeutic testing of urologic tumors. 
  • In vitro chick embryo cell response to strain MC29 avian leukosis virus. 
  • In vitro cultivation of prostatic epithelium. 
  • In vitro culture of epithelial cells derived from urogenital tissues. 
  • In vitro development of inner cell masses isolated from t0/t0 and tW5/tW5 mouse embryos. 
  • In vitro effects of antihypertensive drugs on thromboxane agonist (U46619)-induced vasoconstriction in human internal mammary artery. 
  • In vitro inhibition of collagen cross links by catechol analogs. 
  • In vitro study of drug-induced granulocytopenia. 
  • In vitro synthesis of steroids by a feminising adrenocortical carcinoma: effect of prolactin and other protein hormones. 
  • Inflammation and herpes simplex virus: release of a chemotaxis-generating factor from infected cells. 
  • Inflammation and viral infection: chemotactic activity resulting from the interaction of antiviral antibody and complement with cells infected with herpes simplex virus. 
  • Insulin-like growth factor-1 contributes to neovascularization in age-related macular degeneration. 
  • Intervertebral disc cells exhibit differences in gene expression in alginate and monolayer culture. 
  • Isolation of a human prostate carcinoma cell line (DU 145). 
  • Leptin mediates the parathyroid hormone-related protein paracrine stimulation of fetal lung maturation. 
  • Location-dependent artifact for no measurement using multiwell plates. 
  • Macrophage infiltration of pancreatic acini and islets in acute Kawasaki disease. 
  • Metabolism of benzo[a]pyrene by cultured tracheobronchial tissues from mice, rats, hamsters, bovines and humans. 
  • Microbial colonization of electrocardiographic telemetry systems before and after cleaning. 
  • Minor RNA and other components of host origin intrinsic to avian leukosis virus particles. 
  • Morphologic and immunologic studies of human prostatic carcinoma. 
  • Morphological and biochemical studies of virus (SV40) transformed prostatic tissue. 
  • Necrosis and apoptosis of tumor cells in embolized meningiomas: histopathology and P53, BCL-2, CD-68 immunohistochemistry. 
  • Oncogenic and immunogenic potential of cloned HIX virus in mice and cats. 
  • Peat-based gnotobiotic plant growth systems for Arabidopsis microbiome research. 
  • Pharmacist-managed antimicrobial stewardship program for patients discharged from the emergency department. 
  • Polypeptides of avian RNA tumor viruses. IV. Components of the viral envelope. 
  • Polypeptides of endogenous avian C-type viruses: their detection in the plasma membrane of normal and infected cells. 
  • Postfreeze viability of human renal epithelial carcinoma cells related to phase transformations in ternary solutions. 
  • Production of chemotactic factor and lymphotoxin by human leukocytes stimulated with Herpes simplex virus. 
  • Rapid diagnosis of herpes simplex infection: amplification for electron-microscopy by short-term in vitro replication. 
  • Rapid method for permanent slide preparation of colonies in soft agar cultures. 
  • Reactivity of avian RNA tumor viruses with lectins. 
  • Red blood cell and fibroblast membranes in Duchenne and myotonic muscular dystrophy. 
  • Redistribution and stabilization of cell surface glutamate receptors during synapse formation. 
  • Reduced inspiratory flow attenuates IL-8 release and MAPK activation of lung overstretch. 
  • Regulation of neurogenesis and neuronal differentiation in primary and immortalized cells from mouse olfactory epithelium. 
  • Regulation of translation and transcription of messenger RNA during early embryonic development. 
  • Relaxant effect of diazepam on uterine muscle. 
  • Response of bone marrow to MC29 avian leukosis virus in vitro. 
  • Response of human urothelium to chemical carcinogens in vitro. 
  • Retinoic acid receptor alpha function in vertebrate limb skeletogenesis: a modulator of chondrogenesis. 
  • Rhipicephalus sanguineus: vector of a new spotted fever group rickettsia in the United States. 
  • Ribosomal subunits of Landschütz ascites cells during changes in polysome distribution. 
  • Rodent model of reproductive tract leiomyomata. Establishment and characterization of tumor-derived cell lines. 
  • Role of laminin in localization of human dermal mast cells. 
  • Role of pericellular matrix in development of a mechanically functional neocartilage. 
  • SV40-transformed hamster prostatic tissue: a model of human prostatic malignancy. 
  • Scanning and transmission electron microscopy of human prostatic acinar cells. 
  • Severe acute respiratory syndrome coronavirus 2 environmental contamination in hospital rooms is uncommon using viral culture techniques. 
  • Site-specific phosphorylation and point mutations of telokin modulate its Ca2+-desensitizing effect in smooth muscle. 
  • Strain MC29 avian leukosis virus release by chick embryo cells infected with the agent. 
  • Suppression of the c-Jun N-terminal kinase pathway by 17beta-estradiol can preserve human islet functional mass from proinflammatory cytokine-induced destruction. 
  • Technology Assessment Status Evaluation: tissue sampling during endosonography. February 1997. ASGE. American Society for Gastrointestinal Endoscopy. 
  • Tenascin-C in rat lung: distribution, ontogeny and role in branching morphogenesis. 
  • Tetraethylammonium block of water flux in Aquaporin-1 channels expressed in kidney thin limbs of Henle's loop and a kidney-derived cell line. 
  • The effects of ascorbic acid on cartilage metabolism in guinea pig articular cartilage explants. 
  • The epidemiology and diagnosis of invasive candidiasis among premature infants. 
  • The genesis of seizures in vitro: axon terminal excitability. 
  • The induction of ornithine decarboxylase in cultured hepatoma cells. 
  • The influence of physical structure and charge on neurite extension in a 3D hydrogel scaffold. 
  • The origin of collagenase in periodontal tissues of man. 
  • The polarity and magnitude of ambient charge influences three-dimensional neurite extension from DRGs. 
  • The receptor protein tyrosine phosphatase mu, PTPmu, regulates histogenesis of the chick retina. 
  • The role of NMDA receptors in in vitro epileptogenesis. 
  • The use of histidase for the study of amniotic cell origin and histidinemia. 
  • Tissue culture of epithelium derived from Barrett's oesophagus. 
  • Tissue engineering of functional cardiac muscle: molecular, structural, and electrophysiological studies. 
  • Tissue harmonic imaging sonography of breast lesions: improved margin analysis, conspicuity, and image quality compared to conventional ultrasound. 
  • Tissue-culture surfaces with mixtures of aminated and fluorinated functional groups. Part 1. Synthesis and characterization. 
  • Tissue-culture surfaces with mixtures of aminated and fluorinated functional groups. Part 2. Growth and function of transgenic rat insulinoma cells (betaG I/17). 
  • Transplant approach establishes that kidneys are responsible for serum CSF-1 but require a stimulus in MRL-lpr mice. 
  • Uncompetitive antagonist binding: a biochemical index of activation of the NMDA receptor-coupled ion channel. 
  • Unfocused laser illumination kills dye-targeted mouse neurons by selective photothermolysis. 
  • Viral neoplastic transformation of hamster prostate tissue in vitro. 
  • X-ray crystal structure of acrovestone, a cytotoxic principle from Acronychia pedunculata. 
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  Keywords of People

  • Erickson, Harold Paul, James B. Duke Distinguished Professor Emeritus, Cell Biology
  • Hogan, Brigid L. M., Research Professor of Cell Biology, Cell Biology
  • Kelsoe, Garnett H., James B. Duke Distinguished Professor of Immunology, Integrative Immunobiology
  • Preminger, Glenn Michael, James F. Glenn, M.D. Distinguished Professor of Urology, Surgery, Urology
  • Sunday, Mary Elizabeth Anne, Professor of Pathology, Cell Biology