Immunoprecipitation

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  Subject Areas on Research

  • A RIPK3-caspase 8 complex mediates atypical pro-IL-1β processing. 
  • A possible coagulation-independent mechanism for pregnancy loss involving β(2) glycoprotein 1-dependent antiphospholipid antibodies and CD1d. 
  • A recessive variant of XRCC4 predisposes to non- BRCA1/2 breast cancer in chinese women and impairs the DNA damage response via dysregulated nuclear localization. 
  • A suppressor/enhancer screen in Drosophila reveals a role for wnt-mediated lipid metabolism in primordial germ cell migration. 
  • A two-phase innate host response to alphavirus infection identified by mRNP-tagging in vivo. 
  • ARID1A and PGR proteins interact in the endometrium and reveal a positive correlation in endometriosis. 
  • Acetylation of mitochondrial proteins. 
  • Activated α2-macroglobulin binding to cell surface GRP78 induces T-loop phosphorylation of Akt1 by PDK1 in association with Raptor. 
  • Activity-dependent expression of ELAV/Hu RBPs and neuronal mRNAs in seizure and cocaine brain. 
  • African-derived genetic polymorphisms in TNFAIP3 mediate risk for autoimmunity. 
  • Amyloid precursor protein and amyloid beta-peptide bind to ATP synthase and regulate its activity at the surface of neural cells. 
  • An engineered Saccharomyces cerevisiae strain binds the broadly neutralizing human immunodeficiency virus type 1 antibody 2G12 and elicits mannose-specific gp120-binding antibodies. 
  • An siRNA Screen Identifies the U2 snRNP Spliceosome as a Host Restriction Factor for Recombinant Adeno-associated Viruses. 
  • Ankrd11 is a chromatin regulator involved in autism that is essential for neural development. 
  • Ankyrin-G Inhibits Endocytosis of Cadherin Dimers. 
  • Arabidopsis DDB1-CUL4 ASSOCIATED FACTOR1 forms a nuclear E3 ubiquitin ligase with DDB1 and CUL4 that is involved in multiple plant developmental processes. 
  • Association of calcineurin with the COPI protein Sec28 and the COPII protein Sec13 revealed by quantitative proteomics. 
  • Asymmetric cell divisions promote stratification and differentiation of mammalian skin. 
  • BSTA promotes mTORC2-mediated phosphorylation of Akt1 to suppress expression of FoxC2 and stimulate adipocyte differentiation. 
  • Bcr-Abl-mediated protection from apoptosis downstream of mitochondrial cytochrome c release. 
  • Beta2-adrenergic receptor lysosomal trafficking is regulated by ubiquitination of lysyl residues in two distinct receptor domains. 
  • Binding of activated alpha2-macroglobulin to its cell surface receptor GRP78 in 1-LN prostate cancer cells regulates PAK-2-dependent activation of LIMK. 
  • Bioenergetic defect associated with mKATP channel opening in a mouse model carrying a mitofusin 2 mutation. 
  • Bioluminescence resonance energy transfer identify scaffold protein CNK1 interactions in intact cells. 
  • C-Raf antagonizes apoptosis induced by IFN-alpha in human lung cancer cells by phosphorylation and increase of the intracellular content of elongation factor 1A. 
  • CD1d degradation in Chlamydia trachomatis-infected epithelial cells is the result of both cellular and chlamydial proteasomal activity. 
  • COMMD proteins, a novel family of structural and functional homologs of MURR1. 
  • CYLD inhibits tumorigenesis and metastasis by blocking JNK/AP1 signaling at multiple levels. 
  • Ca2+/calmodulin regulates trafficking of Ca(V)1.2 Ca2+ channels in cultured hippocampal neurons. 
  • Calorie restriction reduces oxidative stress by SIRT3-mediated SOD2 activation. 
  • Calreticulin chaperones regulate functional expression of vomeronasal type 2 pheromone receptors. 
  • Cell cycle dependence of group VIA calcium-independent phospholipase A2 activity. 
  • Cells with pathogenic biallelic mutations in the human MUTYH gene are defective in DNA damage binding and repair. 
  • Clinical characteristics and outcomes in biclonal gammopathies. 
  • Constitutive proteasome-mediated turnover of Bfl-1/A1 and its processing in response to TNF receptor activation in FL5.12 pro-B cells convert it into a prodeath factor. 
  • Coordinate regulation of forskolin-induced cellular proliferation in macrophages by protein kinase A/cAMP-response element-binding protein (CREB) and Epac1-Rap1 signaling: effects of silencing CREB gene expression on Akt activation. 
  • Coordination of replication and transcription along a Drosophila chromosome. 
  • Cse1l is a negative regulator of CFTR-dependent fluid secretion. 
  • Cyclin D1 inhibits peroxisome proliferator-activated receptor gamma-mediated adipogenesis through histone deacetylase recruitment. 
  • Cyclophilin A is localized to the nucleus and controls meiosis in Saccharomyces cerevisiae. 
  • DHX15 is up-regulated in castration-resistant prostate cancer and required for androgen receptor sensitivity to low DHT concentrations. 
  • DO-RIP-seq to quantify RNA binding sites transcriptome-wide. 
  • Deletion of CD4 and CD8 coreceptors permits generation of alphabetaT cells that recognize antigens independently of the MHC. 
  • Delta-like 1-Lysine613 regulates notch signaling. 
  • Design, expression, and immunogenicity of a soluble HIV trimeric envelope fragment adopting a prefusion gp41 configuration. 
  • Differential expression of receptor tyrosine kinases (RTKs) and IGF-I pathway activation in human uterine leiomyomas. 
  • Differential functions for the transcription factor E2A in positive and negative gene regulation in pre-B lymphocytes. 
  • Differential localization of ErbB receptor ensembles influences their signaling in hippocampal neurons. 
  • Dimer-specific immunoprecipitation of active caspase-2 identifies TRAF proteins as novel activators. 
  • Direct interaction of Gas11 with microtubules: implications for the dynein regulatory complex. 
  • Disruption of Gen1 Causes Congenital Anomalies of the Kidney and Urinary Tract in Mice. 
  • Distinct beta-arrestin- and G protein-dependent pathways for parathyroid hormone receptor-stimulated ERK1/2 activation. 
  • Downregulation of NR3A-containing NMDARs is required for synapse maturation and memory consolidation. 
  • Early-life experience decreases drug-induced reinstatement of morphine CPP in adulthood via microglial-specific epigenetic programming of anti-inflammatory IL-10 expression. 
  • Ethanol metabolism modifies hepatic protein acylation in mice. 
  • Exome sequencing identifies somatic gain-of-function PPM1D mutations in brainstem gliomas. 
  • Extracellular trafficking of myocilin in human trabecular meshwork cells. 
  • FBW2 targets GCMa to the ubiquitin-proteasome degradation system. 
  • FMRP targets distinct mRNA sequence elements to regulate protein expression. 
  • FOXO transcription factors control E2F1 transcriptional specificity and apoptotic function. 
  • Formins regulate the actin-related protein 2/3 complex-independent polarization of the centrosome to the immunological synapse. 
  • Functional specialization of beta-arrestin interactions revealed by proteomic analysis. 
  • G protein-coupled receptor kinase regulates dopamine D3 receptor signaling by modulating the stability of a receptor-filamin-beta-arrestin complex. A case of autoreceptor regulation. 
  • GB virus type C envelope protein E2 elicits antibodies that react with a cellular antigen on HIV-1 particles and neutralize diverse HIV-1 isolates. 
  • Generating mammalian sirtuin tools for protein-interaction analysis. 
  • Grb7 binds to Hax-1 and undergoes an intramolecular domain association that offers a model for Grb7 regulation. 
  • Heterotrimeric Galphaq11 co-immunoprecipitates with surface-anchored GRP78 from plasma membranes of alpha2M*-stimulated macrophages. 
  • High-Throughput Odorant Receptor Deorphanization Via Phospho-S6 Ribosomal Protein Immunoprecipitation and mRNA Profiling. 
  • Human centromeric chromatin is a dynamic chromosomal domain that can spread over noncentromeric DNA. 
  • Human cytidine triphosphate synthetase 1 interacting proteins. 
  • Human retroviral gag- and gag-pol-like proteins interact with the transforming growth factor-beta receptor activin receptor-like kinase 1. 
  • Hypothalamic CaMKK2 contributes to the regulation of energy balance. 
  • Identification of Proteins Bound to Dengue Viral RNA In Vivo Reveals New Host Proteins Important for Virus Replication. 
  • Identification of a new transmembrane adaptor protein that constitutively binds Grb2 in B cells. 
  • Identification of calcium-modulating cyclophilin ligand (CAML) as transducer of angiotensin II-mediated nuclear factor of activated T cells (NFAT) activation. 
  • Identification of cellular protein phosphatase-1 regulators. 
  • Identification of deacetylase substrates with the biotin switch approach. 
  • Immunity-related GTPase M (IRGM) proteins influence the localization of guanylate-binding protein 2 (GBP2) by modulating macroautophagy. 
  • Inhibitor-kappaB kinase-beta regulates LPS-induced TNF-alpha production in cardiac myocytes through modulation of NF-kappaB p65 subunit phosphorylation. 
  • Interaction between PKD1L3 and PKD2L1 through their transmembrane domains is required for localization of PKD2L1 at taste pores in taste cells of circumvallate and foliate papillae. 
  • Interaction of Cryptococcus neoformans Rim101 and protein kinase A regulates capsule. 
  • Intercellular trafficking of the nuclear oncoprotein DEK. 
  • Isolation of novel EGFR-specific VHH domains. 
  • Kif7 promotes hedgehog signaling in growth plate chondrocytes by restricting the inhibitory function of Sufu. 
  • Lgr4 and Lgr5 drive the formation of long actin-rich cytoneme-like membrane protrusions. 
  • Life with too much polyprenol: polyprenol reductase deficiency. 
  • Ligation of cell surface-associated glucose-regulated protein 78 by receptor-recognized forms of alpha 2-macroglobulin: activation of p21-activated protein kinase-2-dependent signaling in murine peritoneal macrophages. 
  • Limits in the detection of m⁶ A changes using MeRIP/m⁶ A-seq. 
  • Loss of cell surface TFII-I promotes apoptosis in prostate cancer cells stimulated with activated α₂ -macroglobulin. 
  • MDM2 antagonists overcome intrinsic resistance to CDK4/6 inhibition by inducing p21. 
  • MHC class I-presented tumor antigens identified in ovarian cancer by immunoproteomic analysis are targets for T-cell responses against breast and ovarian cancer. 
  • Mass Spectrometry-Based Proteomics for Analysis of Hydrophilic Phosphopeptides. 
  • Mechanoactivation of the angiotensin II type 1 receptor induces β-arrestin-biased signaling through Gαi coupling. 
  • Merlin facilitates ubiquitination and degradation of transactivation-responsive RNA-binding protein. 
  • Metabolic regulation of Drosophila apoptosis through inhibitory phosphorylation of Dronc. 
  • Mind bomb 1 regulation of cFLIP interactions. 
  • Mouse translation elongation factor eEF1A-2 interacts with Prdx-I to protect cells against apoptotic death induced by oxidative stress. 
  • Native nonmuscle myosin II stability and light chain binding in Drosophila melanogaster. 
  • Negative regulation of TCR signaling by linker for activation of X cells via phosphotyrosine-dependent and -independent mechanisms. 
  • Neuronal ceroid lipofuscinosis: a common pathway? 
  • Neuroprotective effects of reactive oxygen species mediated by BDNF-independent activation of TrkB. 
  • Neutrophil elastase inhibition of cell cycle progression in airway epithelial cells in vitro is mediated by p27kip1. 
  • Nitric oxide-mediated regulation of β-amyloid clearance via alterations of MMP-9/TIMP-1. 
  • Noncentrosomal microtubules and type II myosins potentiate epidermal cell adhesion and barrier formation. 
  • Opposing effects of cancer-type-specific SPOP mutants on BET protein degradation and sensitivity to BET inhibitors. 
  • Osteopontin up-regulates critical epithelial-mesenchymal transition transcription factors to induce an aggressive breast cancer phenotype. 
  • PARalyzer: definition of RNA binding sites from PAR-CLIP short-read sequence data. 
  • Phosphorylation of c-Abl by protein kinase Pak2 regulates differential binding of ABI2 and CRK. 
  • Post-transcriptional regulation of transcript abundance by a conserved member of the tristetraprolin family in Candida albicans. 
  • Preligand assembly domain-mediated ligand-independent association between TRAIL receptor 4 (TR4) and TR2 regulates TRAIL-induced apoptosis. 
  • Prostate cancer-associated SPOP mutations confer resistance to BET inhibitors through stabilization of BRD4. 
  • Protein kinase-zeta interacts with munc18c: role in GLUT4 trafficking. 
  • Protein phosphatase 1 regulates assembly and function of the beta-catenin degradation complex. 
  • Protein phosphatase 5 is required for ATR-mediated checkpoint activation. 
  • Proteolysis-independent downregulation of DELLA repression in Arabidopsis by the gibberellin receptor GIBBERELLIN INSENSITIVE DWARF1. 
  • Proteomic analysis of mitotic RNA polymerase II reveals novel interactors and association with proteins dysfunctional in disease. 
  • Proteomic identification of the cerebral cavernous malformation signaling complex. 
  • Pseudomonas invasion of type I pneumocytes is dependent on the expression and phosphorylation of caveolin-2. 
  • RIP-Chip: the isolation and identification of mRNAs, microRNAs and protein components of ribonucleoprotein complexes from cell extracts. 
  • RNA-binding protein FXR2 regulates adult hippocampal neurogenesis by reducing Noggin expression. 
  • Receptor-specific ubiquitination of beta-arrestin directs assembly and targeting of seven-transmembrane receptor signalosomes. 
  • Redox proteomic identification of HNE-bound mitochondrial proteins in cardiac tissues reveals a systemic effect on energy metabolism after doxorubicin treatment. 
  • Redox-mediated upregulation of hepatocyte iNOS transcription requires coactivator PC4. 
  • Regulation of MEF2 by histone deacetylase 4- and SIRT1 deacetylase-mediated lysine modifications. 
  • Regulation of early adipose commitment by Zfp521. 
  • Regulation of male germ cell cycle arrest and differentiation by DND1 is modulated by genetic background. 
  • Regulation of plant stem cell quiescence by a brassinosteroid signaling module. 
  • Ribotrap : targeted purification of RNA-specific RNPs from cell lysates through immunoaffinity precipitation to identify regulatory proteins and RNAs. 
  • S-Nitrosation of arginase 1 requires direct interaction with inducible nitric oxide synthase 
  • SAP-mediated inhibition of diacylglycerol kinase α regulates TCR-induced diacylglycerol signaling. 
  • SATB1 Expression Governs Epigenetic Repression of PD-1 in Tumor-Reactive T Cells. 
  • SIRT3 deacetylates mitochondrial 3-hydroxy-3-methylglutaryl CoA synthase 2 and regulates ketone body production. 
  • SIRT6 Promotes Hepatic Beta-Oxidation via Activation of PPARα. 
  • Scanning Quadrupole Data-Independent Acquisition, Part B: Application to the Analysis of the Calcineurin-Interacting Proteins during Treatment of Aspergillus fumigatus with Azole and Echinocandin Antifungal Drugs. 
  • Staphylococcus aureus sepsis and mitochondrial accrual of the 8-oxoguanine DNA glycosylase DNA repair enzyme in mice. 
  • Stimulation of GCMa transcriptional activity by cyclic AMP/protein kinase A signaling is attributed to CBP-mediated acetylation of GCMa. 
  • Syncoilin modulates peripherin filament networks and is necessary for large-calibre motor neurons. 
  • Systems analysis identifies melanoma-enriched pro-oncogenic networks controlled by the RNA binding protein CELF1. 
  • TLS interaction with NMDA R1 splice variant in retinal ganglion cell line RGC-5. 
  • Targeting Viral Proteostasis Limits Influenza Virus, HIV, and Dengue Virus Infection. 
  • Tau--an inhibitor of deacetylase HDAC6 function. 
  • Tenascin-C is an innate broad-spectrum, HIV-1-neutralizing protein in breast milk. 
  • The 9-1-1 checkpoint clamp physically interacts with polzeta and is partially required for spontaneous polzeta-dependent mutagenesis in Saccharomyces cerevisiae. 
  • The Chlamydia trachomatis type III secretion chaperone Slc1 engages multiple early effectors, including TepP, a tyrosine-phosphorylated protein required for the recruitment of CrkI-II to nascent inclusions and innate immune signaling. 
  • The Trim39 ubiquitin ligase inhibits APC/CCdh1-mediated degradation of the Bax activator MOAP-1. 
  • The antigen for Hep Par 1 antibody is the urea cycle enzyme carbamoyl phosphate synthetase 1. 
  • The coactivator SRC-1 is an essential coordinator of hepatic glucose production. 
  • The nucleocapsid region of HIV-1 Gag cooperates with the PTAP and LYPXnL late domains to recruit the cellular machinery necessary for viral budding. 
  • The role of MTJ-1 in cell surface translocation of GRP78, a receptor for alpha 2-macroglobulin-dependent signaling. 
  • The telomeric protein TRF2 binds the ATM kinase and can inhibit the ATM-dependent DNA damage response. 
  • Thrombin-cleaved COOH(-) terminal osteopontin peptide binds with cyclophilin C to CD147 in murine breast cancer cells. 
  • Time course of degradation of cardiac troponin I in patients with acute ST-elevation myocardial infarction: the ASSENT-2 troponin substudy. 
  • Tissue transglutaminase regulates focal adhesion kinase/AKT activation by modulating PTEN expression in pancreatic cancer cells. 
  • Translational profiling of cardiomyocytes identifies an early Jak1/Stat3 injury response required for zebrafish heart regeneration. 
  • Tyrosine phosphorylation-independent regulation of lipopolysaccharide-mediated response by the transmembrane adaptor protein LAB. 
  • VCP is essential for mitochondrial quality control by PINK1/Parkin and this function is impaired by VCP mutations. 
  • beta-Arrestin 1 and Galphaq/11 coordinately activate RhoA and stress fiber formation following receptor stimulation. 
  • c-Abl Mediated Tyrosine Phosphorylation of Aha1 Activates Its Co-chaperone Function in Cancer Cells. 
  • hBub1 negatively regulates p53 mediated early cell death upon mitotic checkpoint activation. 
  • miR-126 and miR-126* repress recruitment of mesenchymal stem cells and inflammatory monocytes to inhibit breast cancer metastasis. 
  • p38-MAPK induced dephosphorylation of alpha-tropomyosin is associated with depression of myocardial sarcomeric tension and ATPase activity. 
  • tPA-mediated generation of plasmin is catalyzed by the proteoglycan NG2. 
  • {beta}-Arrestin is crucial for ubiquitination and down-regulation of the insulin-like growth factor-1 receptor by acting as adaptor for the MDM2 E3 ligase. 
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  Keywords of People

  • Alexander, John Hunter Peel, Professor of Medicine, Medicine, Cardiology
  • Erickson, Harold Paul, James B. Duke Distinguished Professor Emeritus, Cell Biology
  • Hirschey, Matthew, Associate Professor of Medicine, Cell Biology
  • McLendon, Roger Edwin, Professor of Pathology, Pathology
  • Patz Jr., Edward F., James and Alice Chen Distinguished Professor of Radiology, Pathology