Green Fluorescent Proteins

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  Subject Areas on Research

  • A Cre-inducible fluorescent reporter for observing apical membrane dynamics. 
  • A Fluorescent Cell-Based System for Imaging Zika Virus Infection in Real-Time. 
  • A GH3-like domain in reaper is required for mitochondrial localization and induction of IAP degradation. 
  • A Gαs DREADD mouse for selective modulation of cAMP production in striatopallidal neurons. 
  • A Mep2-dependent transcriptional profile links permease function to gene expression during pseudohyphal growth in Saccharomyces cerevisiae. 
  • A Modular Method for the High-Yield Synthesis of Site-Specific Protein-Polymer Therapeutics. 
  • A beta-arrestin/green fluorescent protein biosensor for detecting G protein-coupled receptor activation. 
  • A broad competence to respond to SHORT ROOT revealed by tissue-specific ectopic expression. 
  • A circuit model for saccadic suppression in the superior colliculus. 
  • A cis-acting sequence homologous to the yeast filamentation and invasion response element regulates expression of a pectinase gene from the bean pathogen Colletotrichum lindemuthianum. 
  • A common ankyrin-G-based mechanism retains KCNQ and NaV channels at electrically active domains of the axon. 
  • A comprehensive search for calcium binding sites critical for TMEM16A calcium-activated chloride channel activity. 
  • A functional antigenomic promoter for the paramyxovirus simian virus 5 requires proper spacing between an essential internal segment and the 3' terminus. 
  • A gene expression map of the Arabidopsis root. 
  • A genome-wide visual screen reveals a role for sphingolipids and ergosterol in cell surface delivery in yeast. 
  • A grafted ovarian fragment rescues host fertility after chemotherapy. 
  • A gut-brain neural circuit for nutrient sensory transduction. 
  • A high-resolution root spatiotemporal map reveals dominant expression patterns. 
  • A lentiviral vector bearing a reverse intron demonstrates superior expression of both proteins and microRNAs. 
  • A method to measure myocardial calcium handling in adult Drosophila. 
  • A mouse model of adult-onset anaemia due to erythropoietin deficiency. 
  • A next-generation dual-recombinase system for time- and host-specific targeting of pancreatic cancer. 
  • A novel function of VCP (valosin-containing protein; p97) in the control of N-glycosylation of proteins in the endoplasmic reticulum. 
  • A novel imaging method for quantitative Golgi localization reveals differential intra-Golgi trafficking of secretory cargoes. 
  • A novel membrane-anchored Rab5 interacting protein required for homotypic endosome fusion. 
  • A novel rodent mammary window of orthotopic breast cancer for intravital microscopy. 
  • A phase I trial of hyperthermia-induced interleukin-12 gene therapy in spontaneously arising feline soft tissue sarcomas. 
  • A protocol for imaging alternative splicing regulation in vivo using fluorescence reporters in transgenic mice. 
  • A regulatory cascade of three homeobox genes, ceh-10, ttx-3 and ceh-23, controls cell fate specification of a defined interneuron class in C. elegans. 
  • A self-adjuvanting supramolecular vaccine carrying a folded protein antigen. 
  • A small molecule screen identifies a novel compound that induces a homeotic transformation in Hydra. 
  • A stress-sensitive reporter predicts longevity in isogenic populations of Caenorhabditis elegans. 
  • A transgenic toolkit for visualizing and perturbing microtubules reveals unexpected functions in the epidermis. 
  • AAV Gene Therapy for MPS1-associated Corneal Blindness. 
  • Actin-associated neurabin-protein phosphatase-1 complex regulates hippocampal plasticity. 
  • Activation of the canonical bone morphogenetic protein (BMP) pathway during lung morphogenesis and adult lung tissue repair. 
  • Activation-induced endocytosis of the raft-associated transmembrane adaptor protein LAB/NTAL in B lymphocytes: evidence for a role in internalization of the B cell receptor. 
  • Acute in vivo genetic rescue demonstrates that phosphorylation of RIM1alpha serine 413 is not required for mossy fiber long-term potentiation. 
  • Adducin promotes micrometer-scale organization of beta2-spectrin in lateral membranes of bronchial epithelial cells. 
  • Adeno-associated virus-mediated antiangiogenic gene therapy with thrombospondin-1 type 1 repeats and endostatin. 
  • Agonist-specific regulation of delta-opioid receptor trafficking by G protein-coupled receptor kinase and beta-arrestin. 
  • Alphavirus replicon particles as candidate HIV vaccines. 
  • Altered morphology of hippocampal dentate granule cell presynaptic and postsynaptic terminals following conditional deletion of TrkB. 
  • An auxin gradient and maximum in the Arabidopsis root apex shown by high-resolution cell-specific analysis of IAA distribution and synthesis. 
  • An experimental study of GFP-based FRET, with application to intrinsically unstructured proteins. 
  • Analysis of the biologic properties of p230 Bcr-Abl reveals unique and overlapping properties with the oncogenic p185 and p210 Bcr-Abl tyrosine kinases. 
  • Angiotropism of human prostate cancer cells: implications for extravascular migratory metastasis. 
  • Apolipoprotein E-mimetics inhibit neurodegeneration and restore cognitive functions in a transgenic Drosophila model of Alzheimer's disease. 
  • Apparent loss-of-function mutant GPCRs revealed as constitutively desensitized receptors. 
  • Applications for green fluorescent protein (GFP) in the study of host-pathogen interactions. 
  • Applications of gene fusions to green fluorescent protein and flow cytometry to the study of bacterial gene expression in host cells. 
  • Assaying autophagic activity in transgenic GFP-Lc3 and GFP-Gabarap zebrafish embryos. 
  • Assembly dynamics of FtsZ rings in Bacillus subtilis and Escherichia coli and effects of FtsZ-regulating proteins. 
  • Association of calcineurin with the COPI protein Sec28 and the COPII protein Sec13 revealed by quantitative proteomics. 
  • Autocrine BDNF-TrkB signalling within a single dendritic spine. 
  • BDNF-mediated cerebellar granule cell development is impaired in mice null for CaMKK2 or CaMKIV. 
  • BMP signaling and cellular dynamics during regeneration of airway epithelium from basal progenitors. 
  • Bacterial genetics by flow cytometry: rapid isolation of Salmonella typhimurium acid-inducible promoters by differential fluorescence induction. 
  • Barriers to productive transfection of trabecular meshwork cells. 
  • Bcr-Abl-mediated protection from apoptosis downstream of mitochondrial cytochrome c release. 
  • Binding of the beta2 adrenergic receptor to N-ethylmaleimide-sensitive factor regulates receptor recycling. 
  • Bioenergetic analysis of peroxisome proliferator-activated receptor gamma coactivators 1alpha and 1beta (PGC-1alpha and PGC-1beta) in muscle cells. 
  • Bioluminescent virion shells: new tools for quantitation of AAV vector dynamics in cells and live animals. 
  • Bisphenol A affects androgen receptor function via multiple mechanisms. 
  • Blood-derived macrophages infiltrate the retina and activate Muller glial cells under experimental choroidal neovascularization. 
  • Bone marrow chimeras and c-fms conditional ablation (Mafia) mice reveal an essential role for resident myeloid cells in lipopolysaccharide/TLR4-induced corneal inflammation. 
  • Bone marrow-derived progenitor cells contribute to experimental choroidal neovascularization. 
  • Brain radiation injury leads to a dose- and time-dependent recruitment of peripheral myeloid cells that depends on CCR2 signaling. 
  • CD47 regulates the phagocytic clearance and replication of the Plasmodium yoelii malaria parasite. 
  • CDK5 regulates cell adhesion and migration in corneal epithelial cells. 
  • CNS inflammation and bone marrow neuropathy in type 1 diabetes. 
  • CX3CR1 tyrosine sulfation enhances fractalkine-induced cell adhesion. 
  • CX3CR1+ interstitial dendritic cells form a contiguous network throughout the entire kidney. 
  • Ca2+/calmodulin regulates trafficking of Ca(V)1.2 Ca2+ channels in cultured hippocampal neurons. 
  • Calcineurin activation causes retinal ganglion cell degeneration. 
  • Calcium/calmodulin-dependent kinase kinase 2 regulates hematopoietic stem and progenitor cell regeneration. 
  • Cell cycle-dependent expression of thyroid hormone receptor-beta is a mechanism for variable hormone sensitivity. 
  • Cell type-specific expression profiling in plants via cell sorting of protoplasts from fluorescent reporter lines. 
  • Cellular trafficking of G protein-coupled receptor/beta-arrestin endocytic complexes. 
  • Changes in gravitational force induce alterations in gene expression that can be monitored in the live, developing zebrafish heart. 
  • Characterization of basal pseudopod-like processes in ileal and colonic PYY cells. 
  • Characterization of mesonephric development and regeneration using transgenic zebrafish. 
  • Coiled-coil targeting of myocilin to intracellular membranes. 
  • Comparative studies of molecular mechanisms of dopamine D2 and D3 receptors for the activation of extracellular signal-regulated kinase. 
  • Constitutively active alpha-1b adrenergic receptor mutants display different phosphorylation and internalization features. 
  • Cortical inhibition modified by embryonic neural precursors grafted into the postnatal brain. 
  • Cortico-cortical projections in mouse visual cortex are functionally target specific. 
  • Covalent protein-oligonucleotide conjugates by copper-free click reaction. 
  • Cryptococcus neoformans differential gene expression detected in vitro and in vivo with green fluorescent protein. 
  • Cryptococcus neoformans gene expression during murine macrophage infection. 
  • Cse1l is a negative regulator of CFTR-dependent fluid secretion. 
  • Cti6 is an Rpd3-Sin3 histone deacetylase-associated protein required for growth under iron-limiting conditions in Saccharomyces cerevisiae. 
  • Cyclophilin A is localized to the nucleus and controls meiosis in Saccharomyces cerevisiae. 
  • Desmoplakin: an unexpected regulator of microtubule organization in the epidermis. 
  • Development and use of SIV-based Integrase defective lentiviral vector for immunization. 
  • Development of a modified positive selection medium that allows to isolate Aspergillus oryzae strains cured of the integrated niaD-based plasmid. 
  • Differential effects of unfolded protein response pathways on axon injury-induced death of retinal ganglion cells. 
  • Differential functions for the transcription factor E2A in positive and negative gene regulation in pre-B lymphocytes. 
  • Differentiation- and stress-dependent nuclear cytoplasmic redistribution of myopodin, a novel actin-bundling protein. 
  • Discordant on/off switching of gene expression in myocytes during cardiac hypertrophy in vivo. 
  • Disproportionate relationship between APOBEC-1 expression and apolipoprotein B mRNA editing activity. 
  • Disruption of Arp2/3 results in asymmetric structural plasticity of dendritic spines and progressive synaptic and behavioral abnormalities. 
  • Disruption of lysosome function promotes tumor growth and metastasis in Drosophila. 
  • Distinct Kinase-Independent Role of RIPK3 in CD11c+ Mononuclear Phagocytes in Cytokine-Induced Tissue Repair. 
  • Disulfide-mediated dimerization of L1 Ig domains. 
  • Domain 3 of hepatitis C virus core protein is sufficient for intracellular lipid accumulation. 
  • Domain-specific antibodies reveal multiple-site topology of Nup153 within the nuclear pore complex. 
  • Downregulation of NR3A-containing NMDARs is required for synapse maturation and memory consolidation. 
  • Dual labeling of the fibronectin matrix and actin cytoskeleton with green fluorescent protein variants. 
  • Dual labeling of the fibronectin matrix and actin cytoskeleton with green fluorescent protein variants. 
  • Dynamic shuttling and intranuclear mobility of nuclear hormone receptors. 
  • Dynamics and elasticity of the fibronectin matrix in living cell culture visualized by fibronectin-green fluorescent protein. 
  • EGFRvIII-specific chimeric antigen receptor T cells migrate to and kill tumor deposits infiltrating the brain parenchyma in an invasive xenograft model of glioblastoma. 
  • Early recruitment of AU-rich element-containing mRNAs determines their cytosolic fate during iron deficiency. 
  • Efficient and rapid induction of a chronic myelogenous leukemia-like myeloproliferative disease in mice receiving P210 bcr/abl-transduced bone marrow. 
  • Efficient intratracheal delivery of airway epithelial cells in mice and pigs. 
  • Embryonic expression and steroid regulation of brain aromatase cyp19a1b in zebrafish (Danio rerio). 
  • Emergent properties during dorsal closure in Drosophila morphogenesis. 
  • Emergent properties during dorsal closure in Drosophila morphogenesis. 
  • Endocardial cell epithelial-mesenchymal transformation requires Type III TGFβ receptor interaction with GIPC. 
  • Endocytosis and synaptic removal of NR3A-containing NMDA receptors by PACSIN1/syndapin1. 
  • Engineering key components in a synthetic eukaryotic signal transduction pathway. 
  • Enhanced green fluorescent protein as a marker for localizing murine cytomegalovirus in acute and latent infection. 
  • Enhanced inhibition of neointimal hyperplasia by genetically engineered endothelial progenitor cells. 
  • Enhancement of rAAV2-mediated transgene expression in retina cells in vitro and in vivo by coadministration of low-dose chemotherapeutic drugs. 
  • Eradication of solid human breast tumors in nude mice with an intravenously injected light-emitting oncolytic vaccinia virus. 
  • Error-prone polyploid mitosis during normal Drosophila development. 
  • Erythropoietin activates mitochondrial biogenesis and couples red cell mass to mitochondrial mass in the heart. 
  • Evidence for type II cells as cells of origin of K-Ras-induced distal lung adenocarcinoma. 
  • Ex vivo generation of dendritic cells from cryopreserved, post-induction chemotherapy, mobilized leukapheresis from pediatric patients with medulloblastoma. 
  • Expanded polyglutamine domain proteins bind neurofilament and alter the neurofilament network. 
  • Expanded polyglutamine stretches form an 'aggresome'. 
  • Expression of dominant negative Rho-binding domain of Rho-kinase in organ cultured human eye anterior segments increases aqueous humor outflow. 
  • Expression of the Salmonella spp. virulence factor SifA in yeast alters Rho1 activity on peroxisomes. 
  • FACS-optimized mutants of the green fluorescent protein (GFP). 
  • FGF8 signaling is chemotactic for cardiac neural crest cells. 
  • FRAP analysis reveals stabilization of adhesion structures in the epidermis compared to cultured keratinocytes. 
  • Faithful expression of GFP from the PLCbeta2 promoter in a functional class of taste receptor cells. 
  • Feedback control of Swe1p degradation in the yeast morphogenesis checkpoint. 
  • Filamentous fungal-specific septin AspE is phosphorylated in vivo and interacts with actin, tubulin and other septins in the human pathogen Aspergillus fumigatus. 
  • Flow cytometry and bacterial pathogenesis. 
  • Fluorescence linked enzyme chemoproteomic strategy for discovery of a potent and selective DAPK1 and ZIPK inhibitor. 
  • Fluorescence resonance energy transfer analysis of cell surface receptor interactions and signaling using spectral variants of the green fluorescent protein. 
  • Fluorescence-based isolation of bacterial genes expressed within host cells. 
  • Functional maturation of hPSC-derived forebrain interneurons requires an extended timeline and mimics human neural development. 
  • GAP-43 promoter elements in transgenic zebrafish reveal a difference in signals for axon growth during CNS development and regeneration. 
  • GATA2 regulates body water homeostasis through maintaining aquaporin 2 expression in renal collecting ducts. 
  • GFP fusions to a microtubule motor protein to visualize meiotic and mitotic spindle dynamics in Drosophila. 
  • GFP-moesin illuminates actin cytoskeleton dynamics in living tissue and demonstrates cell shape changes during morphogenesis in Drosophila. 
  • Galectin-1 drives lymphoma CD20 immunotherapy resistance: validation of a preclinical system to identify resistance mechanisms. 
  • Generation of neuronal intranuclear inclusions by polyglutamine-GFP: analysis of inclusion clearance and toxicity as a function of polyglutamine length. 
  • Generation of recombinant adeno-associated virus vectors by a complete adenovirus-mediated approach. 
  • Genetic Screen Reveals Link between the Maternal Effect Sterile Gene mes-1 and Pseudomonas aeruginosa-induced Neurodegeneration in Caenorhabditis elegans. 
  • Genetic modification of mesenchymal stem cells overexpressing CCR1 increases cell viability, migration, engraftment, and capillary density in the injured myocardium. 
  • Genome-wide direct target analysis reveals a role for SHORT-ROOT in root vascular patterning through cytokinin homeostasis. 
  • Genome-wide significant risk associations for mucinous ovarian carcinoma. 
  • Gradated assembly of multiple proteins into supramolecular nanomaterials. 
  • Graft-extrinsic cells predominate in vein graft arterialization. 
  • Green fluorescent protein as a tag for molecular motor proteins. 
  • Hand2 regulates extracellular matrix remodeling essential for gut-looping morphogenesis in zebrafish. 
  • Heat shock protein 90 is required for conidiation and cell wall integrity in Aspergillus fumigatus. 
  • Heat-induced gene expression as a novel targeted cancer gene therapy strategy. 
  • Heterogeneity of molecular targets on clonal cancer lines derived from a novel hormone-refractory prostate cancer tumor system. 
  • High-resolution in vivo imaging of fluorescent proteins using window chamber models. 
  • High-resolution, noninvasive longitudinal live imaging of immune responses. 
  • High-speed recording of neural spikes in awake mice and flies with a fluorescent voltage sensor. 
  • How vinculin regulates force transmission. 
  • Human ASPM participates in spindle organisation, spindle orientation and cytokinesis. 
  • Human Trial of a Genetically Modified Herpes Simplex Virus for Rapid Detection of Positive Peritoneal Cytology in the Staging of Pancreatic Cancer. 
  • Human casein kinase Idelta phosphorylation of human circadian clock proteins period 1 and 2. 
  • Human substance P receptor lacking the C-terminal domain remains competent to desensitize and internalize. 
  • Hypoxia-regulated therapeutic gene as a preemptive treatment strategy against ischemia/reperfusion tissue injury. 
  • IL-4 induces differentiation and expansion of Th2 cytokine-producing eosinophils. 
  • IL-6/STAT3 promotes regeneration of airway ciliated cells from basal stem cells. 
  • Identification of Drosophila neuropeptide receptors by G protein-coupled receptors-beta-arrestin2 interactions. 
  • Identification of calcium-modulating cyclophilin ligand (CAML) as transducer of angiotensin II-mediated nuclear factor of activated T cells (NFAT) activation. 
  • Identification of select glucocorticoids as Smoothened agonists: potential utility for regenerative medicine. 
  • Identification of the TBX5 transactivating domain and the nuclear localization signal. 
  • Imaging alternative splicing in living cells. 
  • Imaging molecular pathways: reporter genes. 
  • Imaging the alternative silencing of FGFR2 exon IIIb in vivo. 
  • Immunofluorescent localization of a novel progesterone receptor(s) in a T47D-Y breast cancer cell line lacking genomic progesterone receptor expression. 
  • Improving FRET dynamic range with bright green and red fluorescent proteins. 
  • In Vivo Determination of Mitochondrial Function Using Luciferase-Expressing Caenorhabditis elegans: Contribution of Oxidative Phosphorylation, Glycolysis, and Fatty Acid Oxidation to Toxicant-Induced Dysfunction. 
  • In situ growth of a PEG-like polymer from the C terminus of an intein fusion protein improves pharmacokinetics and tumor accumulation. 
  • In vivo gene delivery and visualization of corneal stromal cells using an adenoviral vector and keratocyte-specific promoter. 
  • In vivo protein expression and immune responses generated by DNA vaccines expressing mycobacterial antigens fused with a reporter protein. 
  • Incorporation of heterologous outer membrane and periplasmic proteins into Escherichia coli outer membrane vesicles. 
  • Individual beta cells within the intact islet differentially respond to glucose. 
  • Inhibition of Cdc42 during mitotic exit is required for cytokinesis. 
  • Inhibition of polyglutamine protein aggregation and cell death by novel peptides identified by phage display screening. 
  • Inhibitory effect of b-AP15 on the 20S proteasome. 
  • Initial stages of tumor cell-induced angiogenesis: evaluation via skin window chambers in rodent models. 
  • Inositol 1,4,5-trisphosphate receptor localization and stability in neonatal cardiomyocytes requires interaction with ankyrin-B. 
  • Inside-out Ca(2+) signalling prompted by STIM1 conformational switch. 
  • Intravascular location of breast cancer cells after spontaneous metastasis to the lung. 
  • Intravital fluorescence facilitates measurement of multiple physiologic functions and gene expression in tumors of live animals. 
  • JNK-induced MCP-1 production in spinal cord astrocytes contributes to central sensitization and neuropathic pain. 
  • L-selectin shedding is activated specifically within transmigrating pseudopods of monocytes to regulate cell polarity in vitro. 
  • Lentiviral mediated gene delivery to the anterior chamber of rodent eyes. 
  • Lentiviral vector design for optimal T cell receptor gene expression in the transduction of peripheral blood lymphocytes and tumor-infiltrating lymphocytes. 
  • Lentiviral vector integration in the human genome induces alternative splicing and generates aberrant transcripts. 
  • Ligand-selective interactions of ER detected in living cells by fluorescence resonance energy transfer. 
  • Ligand-specific dynamics of the androgen receptor at its response element in living cells. 
  • Light-inducible gene regulation with engineered zinc finger proteins. 
  • Localization and function of ADP ribosylation factor A in Aspergillus nidulans. 
  • Localization of brain-derived neurotrophic factor to distinct terminals of mossy fiber axons implies regulation of both excitation and feedforward inhibition of CA3 pyramidal cells. 
  • Loss of basal cells precedes bronchiolitis obliterans-like pathological changes in a murine model of chlorine gas inhalation. 
  • Lung development and repair: contribution of the ciliated lineage. 
  • MKKS/BBS6, a divergent chaperonin-like protein linked to the obesity disorder Bardet-Biedl syndrome, is a novel centrosomal component required for cytokinesis. 
  • Maternal Loss of Ube3a Impairs Experience-Driven Dendritic Spine Maintenance in the Developing Visual Cortex. 
  • Maximal polar growth potential depends on the polarisome component AgSpa2 in the filamentous fungus Ashbya gossypii. 
  • Mechanisms regulating SHORT-ROOT intercellular movement. 
  • Mesenchymal stem cells overexpressing Akt dramatically repair infarcted myocardium and improve cardiac function despite infrequent cellular fusion or differentiation. 
  • Metal-responsive transcription factor (MTF-1) handles both extremes, copper load and copper starvation, by activating different genes. 
  • Microbial colonization induces dynamic temporal and spatial patterns of NF-κB activation in the zebrafish digestive tract. 
  • Microglial morphology and dynamic behavior is regulated by ionotropic glutamatergic and GABAergic neurotransmission. 
  • Mitochondrial dysfunction in NnaD mutant flies and Purkinje cell degeneration mice reveals a role for Nna proteins in neuronal bioenergetics. 
  • Mitochondrial localization of Reaper to promote inhibitors of apoptosis protein degradation conferred by GH3 domain-lipid interactions. 
  • Mobilization of intracellular copper stores by the ctr2 vacuolar copper transporter. 
  • Modulation of phagosome biogenesis by Legionella pneumophila creates an organelle permissive for intracellular growth. 
  • Molecular and genetic approaches for the analysis of C. elegans neuronal development. 
  • Molecular determinants of synapsin targeting to presynaptic terminals. 
  • Molecular determinants underlying the formation of stable intracellular G protein-coupled receptor-beta-arrestin complexes after receptor endocytosis*. 
  • Molecular memory by reversible translocation of calcium/calmodulin-dependent protein kinase II. 
  • Monitoring neural activity with bioluminescence during natural behavior. 
  • Monosynaptic premotor circuit tracing reveals neural substrates for oro-motor coordination. 
  • Mouse germ cell clusters form by aggregation as well as clonal divisions. 
  • Multiple, conserved cryptic recombination signals in VH gene segments: detection of cleavage products only in pro B cells. 
  • Murine granulocyte-macrophage colony-stimulating factor expressed from a bicistronic simian immunodeficiency virus-based integrase-defective lentiviral vector does not enhance T-cell responses in mice. 
  • Muscle-specific RING finger 1 is a bona fide ubiquitin ligase that degrades cardiac troponin I. 
  • Mutations in TMEM216 perturb ciliogenesis and cause Joubert, Meckel and related syndromes. 
  • Neocortical disynaptic inhibition requires somatodendritic integration in interneurons. 
  • Nerve activity-dependent modulation of calcineurin signaling in adult fast and slow skeletal muscle fibers. 
  • Neurabin/protein phosphatase-1 complex regulates dendritic spine morphogenesis and maturation. 
  • Neural cell adhesion molecule-secreting transgenic mice display abnormalities in GABAergic interneurons and alterations in behavior. 
  • Neuregulin-1 enhances depolarization-induced GABA release. 
  • Neuronal cell shape and neurite initiation are regulated by the Ndr kinase SAX-1, a member of the Orb6/COT-1/warts serine/threonine kinase family. 
  • Nogo receptor 1 is expressed by nearly all retinal ganglion cells. 
  • Nonlethal oxidant injury to human retinal pigment epithelium cells causes cell membrane blebbing but decreased MMP-2 activity. 
  • Nonmuscle myosin II generates forces that transmit tension and drive contraction in multiple tissues during dorsal closure. 
  • Novel Sfp1 transcriptional regulation of Saccharomyces cerevisiae gene expression changes during spaceflight. 
  • Novel integrase-defective lentiviral episomal vectors for gene transfer. 
  • Novel sphingosine-1-phosphate receptor modulator KRP203 combined with locally delivered regulatory T cells induces permanent acceptance of pancreatic islet allografts. 
  • Nrf2-mediated protection against 6-hydroxydopamine. 
  • Nuclear localization of NPR1 is required for activation of PR gene expression. 
  • Nuclear repulsion enables division autonomy in a single cytoplasm. 
  • O-glycosylation as a sorting determinant for cell surface delivery in yeast. 
  • Observation of incipient tumor angiogenesis that is independent of hypoxia and hypoxia inducible factor-1 activation. 
  • Oct4 RNA interference induces trophectoderm differentiation in mouse embryonic stem cells. 
  • Oligomerization of expanded-polyglutamine domain fluorescent fusion proteins in cultured mammalian cells. 
  • Optimizing gene transfer to conventional outflow cells in living mouse eyes. 
  • Optogenetic probes. 
  • Out with the old, in with the new? Comparing methods for measuring protein degradation. 
  • PKA/AKAP1 and PP2A/Bβ2 regulate neuronal morphogenesis via Drp1 phosphorylation and mitochondrial bioenergetics. 
  • PSD-95 is essential for hallucinogen and atypical antipsychotic drug actions at serotonin receptors. 
  • Paracrine action accounts for marked protection of ischemic heart by Akt-modified mesenchymal stem cells. 
  • Pcp1p, an Spc110p-related calmodulin target at the centrosome of the fission yeast Schizosaccharomyces pombe. 
  • Pericyte-like location of GFP-tagged melanoma cells: ex vivo and in vivo studies of extravascular migratory metastasis. 
  • Perivascular mural cells of the mouse choroid demonstrate morphological diversity that is correlated to vasoregulatory function. 
  • Persistent genetic instability in cancer cells induced by non-DNA-damaging stress exposures. 
  • Plasticity of Hopx(+) type I alveolar cells to regenerate type II cells in the lung. 
  • Polyglutamine domain proteins with expanded repeats bind neurofilament, altering the neurofilament network. 
  • Predicting longevity in C. elegans: fertility, mobility and gene expression. 
  • Preventing stem cell incorporation into choroidal neovascularization by targeting homing and attachment factors. 
  • Primary mesenchyme cell patterning during the early stages following ingression. 
  • Probing the domain structure of FtsZ by random truncation and insertion of GFP. 
  • Production of recombinant adeno-associated viral vectors and use for in vitro and in vivo administration. 
  • Proteasome inhibition by chronic oxidative stress in human trabecular meshwork cells. 
  • Proteasome overload is a common stress factor in multiple forms of inherited retinal degeneration. 
  • Protection against Mycobacterium avium by DNA vaccines expressing mycobacterial antigens as fusion proteins with green fluorescent protein. 
  • Protein synthesis inhibitors and the chemical chaperone TMAO reverse endoplasmic reticulum perturbation induced by overexpression of the iodide transporter pendrin. 
  • Quantitative enumeration of vascular smooth muscle cells and endothelial cells derived from bone marrow precursors in experimental choroidal neovascularization. 
  • RAG2:GFP knockin mice reveal novel aspects of RAG2 expression in primary and peripheral lymphoid tissues. 
  • RANK, RANKL, OPG, and M-CSF expression in stromal cells during corneal wound healing. 
  • Rab10 regulates membrane transport through early endosomes of polarized Madin-Darby canine kidney cells. 
  • RacGap50C negatively regulates wingless pathway activity during Drosophila embryonic development. 
  • Radiation activates HIF-1 to regulate vascular radiosensitivity in tumors: role of reoxygenation, free radicals, and stress granules. 
  • Rap1 and Canoe/afadin are essential for establishment of apical-basal polarity in the Drosophila embryo. 
  • Rapid assembly dynamics of the Escherichia coli FtsZ-ring demonstrated by fluorescence recovery after photobleaching. 
  • Rapid selection of tetracycline-controlled inducible cell lines using a green fluorescent-transactivator fusion protein. 
  • Real-time imaging of morphogenetic movements in Drosophila using Gal4-UAS-driven expression of GFP fused to the actin-binding domain of moesin. 
  • Receptor-specific ubiquitination of beta-arrestin directs assembly and targeting of seven-transmembrane receptor signalosomes. 
  • Regeneration Genetics. 
  • Regeneration of amputated zebrafish fin rays from de novo osteoblasts. 
  • Regulated heat shock protein 27 expression in human retinal pigment epithelium. 
  • Regulation of ZAP-70 intracellular localization: visualization with the green fluorescent protein. 
  • Regulation of the Saccharomyces cerevisiae Slt2 kinase pathway by the stress-inducible Sdp1 dual specificity phosphatase. 
  • Repressing a repressor: gibberellin-induced rapid reduction of the RGA protein in Arabidopsis. 
  • Retinal pigment epithelium protection from oxidant-mediated loss of MMP-2 activation requires both MMP-14 and TIMP-2. 
  • RhoG regulates endothelial apical cup assembly downstream from ICAM1 engagement and is involved in leukocyte trans-endothelial migration. 
  • Role of decreased levels of lipid phosphate phosphatase-1 in accumulation of lysophosphatidic acid in ovarian cancer. 
  • Rvs161p and sphingolipids are required for actin repolarization following salt stress. 
  • S-Nitrosoglutathione Reductase Dysfunction Contributes to Obesity-Associated Hepatic Insulin Resistance via Regulating Autophagy. 
  • SIRT1 protects against microglia-dependent amyloid-beta toxicity through inhibiting NF-kappaB signaling. 
  • Salmonella typhimurium proliferates and establishes a persistent infection in the intestine of Caenorhabditis elegans. 
  • Sapap3 deletion causes mGluR5-dependent silencing of AMPAR synapses. 
  • Scanning Quadrupole Data-Independent Acquisition, Part B: Application to the Analysis of the Calcineurin-Interacting Proteins during Treatment of Aspergillus fumigatus with Azole and Echinocandin Antifungal Drugs. 
  • Selective modification of antigen-specific T cells by RNA electroporation. 
  • Signaling, desensitization, and trafficking of G protein-coupled receptors revealed by green fluorescent protein conjugates. 
  • Simultaneous stretching and contraction of stress fibers in vivo. 
  • Simultaneous stretching and contraction of stress fibers in vivo. 
  • Sonic hedgehog is required early in pancreatic islet development. 
  • Sortase-catalyzed initiator attachment enables high yield growth of a stealth polymer from the C terminus of a protein. 
  • Sorting human beta-cells consequent to targeted expression of green fluorescent protein. 
  • Sox2 expression in Schwann cells inhibits myelination in vivo and induces influx of macrophages to the nerve. 
  • Spatial coordination between stem cell activity and cell differentiation in the root meristem. 
  • Sphingosine-1-phosphate phosphohydrolase regulates endoplasmic reticulum-to-golgi trafficking of ceramide. 
  • Sphingosine-phosphate lyase enhances stress-induced ceramide generation and apoptosis. 
  • Spindle dynamics during meiosis in Drosophila oocytes. 
  • Structural evidence that the P/Q domain of ZipA is an unstructured, flexible tether between the membrane and the C-terminal FtsZ-binding domain. 
  • Survival of retinal pigment epithelium after exposure to prolonged oxidative injury: a detailed gene expression and cellular analysis. 
  • Targeted beta-adrenergic receptor kinase (betaARK1) inhibition by gene transfer in failing human hearts. 
  • Targeted inactivation of the isoprenylcysteine carboxyl methyltransferase gene causes mislocalization of K-Ras in mammalian cells. 
  • Targeting inhibition of GluR1 Ser845 phosphorylation with an RNA aptamer that blocks AMPA receptor trafficking. 
  • Temporally distinct and ligand-specific recruitment of nuclear receptor-interacting peptides and cofactors to subnuclear domains containing the estrogen receptor. 
  • Temporomandibular joint pain: a critical role for Trpv4 in the trigeminal ganglion. 
  • The Arabidopsis RGA gene encodes a transcriptional regulator repressing the gibberellin signal transduction pathway. 
  • The Arabidopsis purple acid phosphatase AtPAP10 is predominantly associated with the root surface and plays an important role in plant tolerance to phosphate limitation. 
  • The C2 domain protein Cts1 functions in the calcineurin signaling circuit during high-temperature stress responses in Cryptococcus neoformans. 
  • The CD14 receptor does not mediate entry of Mycobacterium tuberculosis into human mononuclear phagocytes. 
  • The DELLA motif is essential for gibberellin-induced degradation of RGA. 
  • The L84F polymorphic variant of human O6-methylguanine-DNA methyltransferase alters stability in U87MG glioma cells but not temozolomide sensitivity. 
  • The ankyrin-B C-terminal domain determines activity of ankyrin-B/G chimeras in rescue of abnormal inositol 1,4,5-trisphosphate and ryanodine receptor distribution in ankyrin-B (-/-) neonatal cardiomyocytes. 
  • The anti-helminthic niclosamide inhibits Wnt/Frizzled1 signaling. 
  • The beta2-adrenergic receptor/betaarrestin complex recruits the clathrin adaptor AP-2 during endocytosis. 
  • The candidate sour taste receptor, PKD2L1, is expressed by type III taste cells in the mouse. 
  • The cellular and synaptic location of activated TrkB in mouse hippocampus during limbic epileptogenesis. 
  • The effect of surface agitation on ultrasound-mediated gene transfer in vitro. 
  • The effect of surface agitation on ultrasound-mediated gene transfer in vitro. 
  • The effects of crosslinking of scaffolds engineered from cartilage ECM on the chondrogenic differentiation of MSCs. 
  • The glucocorticoid receptor interacting protein 1 (GRIP1) localizes in discrete nuclear foci that associate with ND10 bodies and are enriched in components of the 26S proteasome. 
  • The homeodomain protein HOXB13 regulates the cellular response to androgens. 
  • The inducibly expressed GTPase localizes to the endoplasmic reticulum, independently of GTP binding. 
  • The integrin-linked kinase regulates cell morphology and motility in a rho-associated kinase-dependent manner. 
  • The molecular identities of the Caenorhabditis elegans intraflagellar transport genes dyf-6, daf-10 and osm-1. 
  • The netrin receptor DCC focuses invadopodia-driven basement membrane transmigration in vivo. 
  • The obligate intracellular pathogen Chlamydia trachomatis targets host lipid droplets. 
  • The stability of the G protein-coupled receptor-beta-arrestin interaction determines the mechanism and functional consequence of ERK activation. 
  • Thrombospondin-4 contributes to spinal sensitization and neuropathic pain states. 
  • Trafficking of the vesicular acetylcholine transporter in SN56 cells: a dynamin-sensitive step and interaction with the AP-2 adaptor complex. 
  • Transactivation of the epidermal growth factor receptor by angiotensin II in glomerular podocytes. 
  • Transgenic expression of green fluorescence protein can cause dilated cardiomyopathy. 
  • Transient hypoxia stimulates mitochondrial biogenesis in brain subcortex by a neuronal nitric oxide synthase-dependent mechanism. 
  • Transient opening of fibronectin type III (FNIII) domains: the interaction of the third FNIII domain of FN with anastellin. 
  • Transiently expressed ATG16L1 inhibits autophagosome biogenesis and aberrantly targets RAB11-positive recycling endosomes. 
  • Transsynaptic Tracing from Peripheral Targets with Pseudorabies Virus Followed by Cholera Toxin and Biotinylated Dextran Amines Double Labeling. 
  • Tribbles homolog 2 inactivates C/EBPalpha and causes acute myelogenous leukemia. 
  • Two and three-dimensional gene transfer from enzymatically degradable hydrogel scaffolds. 
  • Two novel alleles of tottering with distinct Ca(v)2.1 calcium channel neuropathologies. 
  • Tyrosine 1101 of Tie2 is the major site of association of p85 and is required for activation of phosphatidylinositol 3-kinase and Akt. 
  • Ultra-high expression of a thermally responsive recombinant fusion protein in E. coli. 
  • Unc-51-like kinase 1/2-mediated endocytic processes regulate filopodia extension and branching of sensory axons. 
  • Understanding the elasticity of fibronectin fibrils: unfolding strengths of FN-III and GFP domains measured by single molecule force spectroscopy. 
  • Unfolded protein response genes regulated by CED-1 are required for Caenorhabditis elegans innate immunity. 
  • Use of Hes1-GFP reporter mice to assess activity of the Hes1 promoter in bone cells under chronic inflammation. 
  • VSV-G pseudotyped, MuLV-based, semi-replication-competent retrovirus for cancer treatment. 
  • Visualization and characterization of inflammatory cell recruitment and migration through the corneal stroma in endotoxin-induced keratitis. 
  • Visualization of dynamic trafficking of a protein kinase C betaII/green fluorescent protein conjugate reveals differences in G protein-coupled receptor activation and desensitization. 
  • Visualizing hidden heterogeneity in isogenic populations of C. elegans. 
  • Visualizing receptor endocytosis and trafficking. 
  • WRP/srGAP3 facilitates the initiation of spine development by an inverse F-BAR domain, and its loss impairs long-term memory. 
  • Yolk-sac-derived macrophages regulate fetal testis vascularization and morphogenesis. 
  • Zebrafish: a see-through host and a fluorescent toolbox to probe host-pathogen interaction. 
  • [Studying pathogenesis of Alzheimer's disease in a Drosophila melanogaster model: human APP overexpression in the brain of transgenic flies leads to deficit of the synaptic protein synaptotagmin]. 
  • beta-Arrestin 1 and Galphaq/11 coordinately activate RhoA and stress fiber formation following receptor stimulation. 
  • beta-Arrestin-mediated PDE4 cAMP phosphodiesterase recruitment regulates beta-adrenoceptor switching from Gs to Gi. 
  • c-Myc creates an activation loop by transcriptionally repressing its own functional inhibitor, hMad4, in young fibroblasts, a loop lost in replicatively senescent fibroblasts. 
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  Keywords of People

  • Aballay, Alejandro, Adjunct Professor in the Department of Molecular Genetics and Microbiology, Molecular Genetics and Microbiology
  • Bennett, Vann, George B. Geller Professor for Research in Molecular Biology in the School of Medicine, Duke Cancer Institute
  • Berchuck, Andrew, James M. Ingram Professor of Gynecologic Oncology, Obstetrics and Gynecology, Gynecologic Oncology
  • Davis, Erica Ellen, Associate Professor of Pediatrics, Cell Biology
  • Erickson, Harold Paul, James B. Duke Professor of Cell Biology, Biomedical Engineering
  • Hogan, Brigid L. M., George Barth Geller Professor, Cell Biology
  • Juvvadi, Praveen Rao, Assistant Professor in Pediatrics, Pediatrics, Infectious Diseases
  • Liton, Paloma Borrajo, Associate Professor in Ophthalmology, Pathology
  • McDonnell, Donald Patrick, Chair, Department of Pharmacology & Cancer Biology, Duke Innovation & Entrepreneurship
  • Milano, Carmelo Alessio, Professor of Surgery, Surgery, Cardiovascular and Thoracic Surgery
  • Pitt, Geoffrey Stuart, Adjunct Professor in the Department of Medicine, Medicine, Cardiology
  • Soderling, Scott Haydn, Professor in Cell Biology, Cell Biology
  • Taylor, Gregory Alan, Associate Professor in Medicine, Immunology
  • Tsalik, Ephraim, Associate Professor of Medicine, Molecular Genetics and Microbiology
  • Velkey, John Matthew, Assistant Professor of the Practice of Medical Education in the Department of Cell Biology, Cell Biology
  • Zhong, Pei, Professor in the Department of Mechanical Enginnering and Materials Science, Mechanical Engineering and Materials Science