Biocatalysis

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  Subject Areas on Research

  • A Class of Reactive Acyl-CoA Species Reveals the Non-enzymatic Origins of Protein Acylation. 
  • A hot oxidant, 3-NO2Y122 radical, unmasks conformational gating in ribonucleotide reductase. 
  • ADAM9 inhibition increases membrane activity of ADAM10 and controls α-secretase processing of amyloid precursor protein. 
  • Acyl carrier protein-specific 4'-phosphopantetheinyl transferase activates 10-formyltetrahydrofolate dehydrogenase. 
  • Allosteric cooperation in a de novo-designed two-domain protein. 
  • Bacterial Growth Inhibition Screen (BGIS): harnessing recombinant protein toxicity for rapid and unbiased interrogation of protein function. 
  • Cardioprotective effects of iron chelator HAPI and ROS-activated boronate prochelator BHAPI against catecholamine-induced oxidative cellular injury. 
  • Catalytic mechanism of 4-oxalocrotonate tautomerase: significances of protein-protein interactions on proton transfer pathways. 
  • Characterization of the PMT gene family in Cryptococcus neoformans. 
  • Chasing acyl carrier protein through a catalytic cycle of lipid A production. 
  • Chemical and enzyme-assisted syntheses of norbuprenorphine-3-β-D-glucuronide. 
  • Cryo-EM Analysis of the Conformational Landscape of Human P-glycoprotein (ABCB1) During its Catalytic Cycle. 
  • Cytosolic accumulation of small nucleolar RNAs (snoRNAs) is dynamically regulated by NADPH oxidase. 
  • Detection of necrosis by release of lactate dehydrogenase activity. 
  • Differential loss of prolyl isomerase or chaperone activity of Ran-binding protein 2 (Ranbp2) unveils distinct physiological roles of its cyclophilin domain in proteostasis. 
  • Dual-Enzyme-Loaded Multifunctional Hybrid Nanogel System for Pathological Responsive Ultrasound Imaging and T2-Weighted Magnetic Resonance Imaging. 
  • Emi2-mediated inhibition of E2-substrate ubiquitin transfer by the anaphase-promoting complex/cyclosome through a D-box-independent mechanism. 
  • Enzymatic Synthesis of Nucleobase-Modified Single-Stranded DNA Offers Tunable Resistance to Nuclease Degradation. 
  • Enzymatic polymerization of high molecular weight DNA amphiphiles that self-assemble into star-like micelles. 
  • Enzymatic properties of ALDH1L2, a mitochondrial 10-formyltetrahydrofolate dehydrogenase. 
  • Enzyme redesign guided by cancer-derived IDH1 mutations. 
  • Equilibration of tyrosyl radicals (Y356•, Y731•, Y730•) in the radical propagation pathway of the Escherichia coli class Ia ribonucleotide reductase. 
  • Functional Class I and II Amino Acid-activating Enzymes Can Be Coded by Opposite Strands of the Same Gene. 
  • Glutamate 350 Plays an Essential Role in Conformational Gating of Long-Range Radical Transport in Escherichia coli Class Ia Ribonucleotide Reductase. 
  • HRP-mediated polymerization forms tough nanocomposite hydrogels with high biocatalytic performance. 
  • Hybrid photopatterned enzymatic reaction (HyPER) for in situ cell manipulation 
  • Identification of a cyclic nucleotide as a cryptic intermediate in molybdenum cofactor biosynthesis. 
  • Identification of undecaprenyl phosphate-beta-D-galactosamine in Francisella novicida and its function in lipid A modification. 
  • In vitro hepatic metabolism explains higher clearance of voriconazole in children versus adults: role of CYP2C19 and flavin-containing monooxygenase 3. 
  • Interplay of catalysis, fidelity, threading, and processivity in the exo- and endonucleolytic reactions of human exonuclease I. 
  • Investigating the Role of Class I Adenylate-Forming Enzymes in Natural Product Biosynthesis. 
  • Length Specificity and Polymerization Mechanism of (1,3)-β-d-Glucan Synthase in Fungal Cell Wall Biosynthesis. 
  • Localized and Controlled Delivery of Nitric Oxide to the Conventional Outflow Pathway via Enzyme Biocatalysis: Toward Therapy for Glaucoma. 
  • Paradigm Shift for Radical S-Adenosyl-l-methionine Reactions: The Organometallic Intermediate Ω Is Central to Catalysis. 
  • Site-specific incorporation of 3-nitrotyrosine as a probe of pKa perturbation of redox-active tyrosines in ribonucleotide reductase. 
  • Structural and Functional Characterization of Sulfonium Carbon-Oxygen Hydrogen Bonding in the Deoxyamino Sugar Methyltransferase TylM1. 
  • Structural and In Vivo Studies on Trehalose-6-Phosphate Synthase from Pathogenic Fungi Provide Insights into Its Catalytic Mechanism, Biological Necessity, and Potential for Novel Antifungal Drug Design. 
  • Structural reorganization and preorganization in enzyme active sites: comparisons of experimental and theoretically ideal active site geometries in the multistep serine esterase reaction cycle. 
  • Structure of the essential Haemophilus influenzae UDP-diacylglucosamine pyrophosphohydrolase LpxH in lipid A biosynthesis. 
  • Structure-Activity Relationship of Sulfonyl Piperazine LpxH Inhibitors Analyzed by an LpxE-Coupled Malachite Green Assay. 
  • Structures of the Bacillus subtilis glutamine synthetase dodecamer reveal large intersubunit catalytic conformational changes linked to a unique feedback inhibition mechanism. 
  • Structures of trehalose-6-phosphate phosphatase from pathogenic fungi reveal the mechanisms of substrate recognition and catalysis. 
  • The dCMP transferase activity of yeast Rev1 is biologically relevant during the bypass of endogenously generated AP sites. 
  • Thermal responsive microgels as recyclable carriers to immobilize active proteins with enhanced nonaqueous biocatalytic performance. 
  • Unique functional and structural properties of the LRRK2 protein ATP-binding pocket. 
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  Keywords of People

  • Deshusses, Marc, Professor of Civil and Environmental Engineering, Nicholas Institute-Energy Initiative
  • Ferreira, Paulo Alexandre, Associate Professor in Ophthalmology, Pathology