Protein Multimerization

• 

  Subject Areas on Research

  • A DNA mimic: the structure and mechanism of action for the anti-repressor protein AbbA. 
  • A Sweet Embrace: Control of Protein-Protein Interactions by O-Linked β-N-Acetylglucosamine. 
  • A cell-based multiplex immunoassay platform using fluorescent protein-barcoded reporter cell lines. 
  • A chimeric kinesin-1 head/kinesin-5 tail motor switches between diffusive and processive motility. 
  • A constitutively active GPCR governs morphogenic transitions in Cryptococcus neoformans. 
  • A cut above the other caspases. 
  • A graphical method for analyzing distance restraints using residual dipolar couplings for structure determination of symmetric protein homo-oligomers. 
  • ADF/cofilin promotes invadopodial membrane recycling during cell invasion in vivo. 
  • ALS- and FTD-associated missense mutations in TBK1 differentially disrupt mitophagy. 
  • Accelerated neurodegeneration through chaperone-mediated oligomerization of tau. 
  • Actin is required for IFT regulation in Chlamydomonas reinhardtii. 
  • Amanitin-resistant RNA polymerase II mutations are in the enzyme's largest subunit. 
  • An estimate to the first approximation of microtubule rupture force. 
  • Androgen receptor variant-driven prostate cancer: clinical implications and therapeutic targeting. 
  • Ankyrin-G Inhibits Endocytosis of Cadherin Dimers. 
  • Antibody Responses Elicited by Immunization with BG505 Trimer Immune Complexes. 
  • Antibody responses induced by SHIV infection are more focused than those induced by soluble native HIV-1 envelope trimers in non-human primates. 
  • Antigenicity and Immunogenicity of HIV-1 Envelope Trimers Complexed to a Small-Molecule Viral Entry Inhibitor. 
  • Atomic force microscopy captures MutS tetramers initiating DNA mismatch repair. 
  • BMP antagonism protects Nodal signaling in the gastrula to promote the tissue interactions underlying mammalian forebrain and craniofacial patterning. 
  • Brain spectrin. Isolation of subunits and formation of hybrids with erythrocyte spectrin subunits. 
  • Calmodulin dissociation regulates Myo5 recruitment and function at endocytic sites. 
  • Cancer. The transcription factor GABP selectively binds and activates the mutant TERT promoter in cancer. 
  • Characterization and structure of the manganese-responsive transcriptional regulator ScaR. 
  • Characterization of SARS2 Nsp15 nuclease activity reveals it's mad about U. 
  • Characterization of a trimeric MPER containing HIV-1 gp41 antigen. 
  • Clonal analysis of human anti-V3 monoclonal antibodies selected by a V3 tetramer. 
  • Comparison of the physical and functional properties of the 48-subunit native molecule and the 24- and 12-subunit dissociation intermediates of Limulus polyphemus hemocyanin. 
  • Computational design and elaboration of a de novo heterotetrameric alpha-helical protein that selectively binds an emissive abiological (porphinato)zinc chromophore. 
  • Cryo-EM structure of a mitochondrial calcium uniporter. 
  • Crystal structure, conformational fixation and entry-related interactions of mature ligand-free HIV-1 Env. 
  • Dependence of leucine-rich repeat kinase 2 (LRRK2) kinase activity on dimerization. 
  • Determinants of Tenascin-C and HIV-1 envelope binding and neutralization. 
  • Developing an HIV vaccine. 
  • Dimer-specific immunoprecipitation of active caspase-2 identifies TRAF proteins as novel activators. 
  • Dimerization and ubiquitin mediated recruitment of A20, a complex deubiquitinating enzyme. 
  • Dimerization deficiency of enigmatic retinitis pigmentosa-linked rhodopsin mutants. 
  • Direct observation of multimer stabilization in the mechanical unfolding pathway of a protein undergoing oligomerization. 
  • Discrete fracture patterns of virus shells reveal mechanical building blocks. 
  • Disruption of arterial perivascular drainage of amyloid-β from the brains of mice expressing the human APOE ε4 allele. 
  • Disruption of the HIV-1 Envelope allosteric network blocks CD4-induced rearrangements. 
  • Disruption of the β1L Isoform of GABP Reverses Glioblastoma Replicative Immortality in a TERT Promoter Mutation-Dependent Manner. 
  • Dynamics of Forced Nucleosome Unraveling and Role of Nonuniform Histone-DNA Interactions 
  • Efficacy of the adjuvanted subunit protein COVID-19 vaccine, SCB-2019: a phase 2 and 3 multicentre, double-blind, randomised, placebo-controlled trial. 
  • Electron tomography of fiber cell cytoplasm and dense cores of multilamellar bodies from human age-related nuclear cataracts. 
  • Elicitation of Robust Tier 2 Neutralizing Antibody Responses in Nonhuman Primates by HIV Envelope Trimer Immunization Using Optimized Approaches. 
  • Enhancing and shaping the immunogenicity of native-like HIV-1 envelope trimers with a two-component protein nanoparticle. 
  • Epitopes for neutralizing antibodies induced by HIV-1 envelope glycoprotein BG505 SOSIP trimers in rabbits and macaques. 
  • FKBP12 dimerization mutations effect FK506 binding and differentially alter calcineurin inhibition in the human pathogen Aspergillus fumigatus. 
  • Fatty acid synthase inhibition engages a novel caspase-2 regulatory mechanism to induce ovarian cancer cell death. 
  • Fibronectin Conformation and Assembly: Analysis of Fibronectin Deletion Mutants and Fibronectin Glomerulopathy (GFND) Mutants. 
  • Flexible stereospecific interactions and composition within nucleoprotein complexes assembled on the TCR alpha gene enhancer. 
  • Folding Landscape of Mutant Huntingtin Exon1: Diffusible Multimers, Oligomers and Fibrils, and No Detectable Monomer. 
  • FtsZ Protofilament Curvature Is the Opposite of Tubulin Rings. 
  • Generation and Characterization of a Bivalent HIV-1 Subtype C gp120 Protein Boost for Proof-of-Concept HIV Vaccine Efficacy Trials in Southern Africa. 
  • GlcNAc-1-P-transferase-tunicamycin complex structure reveals basis for inhibition of N-glycosylation. 
  • Grc3 programs the essential endoribonuclease Las1 for specific RNA cleavage. 
  • Guides to the final frontier of the cytoskeleton: septins in filamentous fungi. 
  • HIV-1 Envelope Conformation, Allostery, and Dynamics. 
  • HIV-1 VACCINES. HIV-1 neutralizing antibodies induced by native-like envelope trimers. 
  • HIV-1 envelope glycoprotein trimers display open quaternary conformation when bound to the gp41 membrane-proximal external-region-directed broadly neutralizing antibody Z13e1. 
  • HIV-1-neutralizing antibody induced by simian adenovirus- and poxvirus MVA-vectored BG505 native-like envelope trimers. 
  • Haptoglobin alters oxygenation and oxidation of hemoglobin and decreases propagation of peroxide-induced oxidative reactions. 
  • Heterodimerization among thyroid hormone receptor, retinoic acid receptor, retinoid X receptor, chicken ovalbumin upstream promoter transcription factor, and an endogenous liver protein. 
  • High-affinity interaction of poly(ADP-ribose) and the human DEK oncoprotein depends upon chain length. 
  • Highly photostable fluorescent labeling of proteins in live cells using exchangeable coiled coils heterodimerization. 
  • HipBA-promoter structures reveal the basis of heritable multidrug tolerance. 
  • Hypoxia inhibits induction of aryl hydrocarbon receptor activity in topminnow hepatocarcinoma cells in an ARNT-dependent manner. 
  • Immunization of rabbits with highly purified, soluble, trimeric human immunodeficiency virus type 1 envelope glycoprotein induces a vigorous B cell response and broadly cross-reactive neutralization. 
  • Immunogenicity in Rabbits of HIV-1 SOSIP Trimers from Clades A, B, and C, Given Individually, Sequentially, or in Combination. 
  • In vitro reconstitution of septin assemblies on supported lipid bilayers. 
  • Inside-out Z rings--constriction with and without GTP hydrolysis. 
  • Insight into F plasmid DNA segregation revealed by structures of SopB and SopB-DNA complexes. 
  • Interaction with cellular CD4 exposes HIV-1 envelope epitopes targeted by antibody-dependent cell-mediated cytotoxicity. 
  • Interactions of nucleolin and ribosomal protein L26 (RPL26) in translational control of human p53 mRNA. 
  • International Workshop at the Nobel Forum, Karolinska Institutet on G protein-coupled receptors: finding the words to describe monomers, oligomers, and their molecular mechanisms and defining their meaning. Can a consensus be reached? 
  • Kinesin walks the line: single motors observed by atomic force microscopy. 
  • LRRK2 autophosphorylation enhances its GTPase activity. 
  • Mapping the immunogenic landscape of near-native HIV-1 envelope trimers in non-human primates. 
  • Mcm2-7 Is an Active Player in the DNA Replication Checkpoint Signaling Cascade via Proposed Modulation of Its DNA Gate. 
  • Microtubule-driven multimerization recruits ase1p onto overlapping microtubules. 
  • Mimicry of an HIV broadly neutralizing antibody epitope with a synthetic glycopeptide. 
  • Molecular mechanism by which the nucleoid occlusion factor, SlmA, keeps cytokinesis in check. 
  • Monitoring of receptor dimerization using plasmonic coupling of gold nanoparticles. 
  • NURR1 expression regulates retinal pigment epithelial-mesenchymal transition and age-related macular degeneration phenotypes. 
  • Nature of Amorphous Hydrophilic Block Affects Self-Assembly of an Artificial Viral Coat Polypeptide. 
  • Neutralization-guided design of HIV-1 envelope trimers with high affinity for the unmutated common ancestor of CH235 lineage CD4bs broadly neutralizing antibodies. 
  • Neutralizing Antibody Induction by HIV-1 Envelope Glycoprotein SOSIP Trimers on Iron Oxide Nanoparticles May Be Impaired by Mannose Binding Lectin. 
  • Nitric-oxide synthase forms N-NO-pterin and S-NO-cys: implications for activity, allostery, and regulation. 
  • Nucleolin mediates nucleosome disruption critical for DNA double-strand break repair. 
  • Overcoming the solubility limit with solubility-enhancement tags: successful applications in biomolecular NMR studies. 
  • Piezo proteins are pore-forming subunits of mechanically activated channels. 
  • Plasmid protein TubR uses a distinct mode of HTH-DNA binding and recruits the prokaryotic tubulin homolog TubZ to effect DNA partition. 
  • Prefusion structure of trimeric HIV-1 envelope glycoprotein determined by cryo-electron microscopy. 
  • Presenting native-like HIV-1 envelope trimers on ferritin nanoparticles improves their immunogenicity. 
  • Primate-specific melanoma antigen-A11 regulates isoform-specific human progesterone receptor-B transactivation. 
  • Probing domain interactions in soluble guanylate cyclase. 
  • Quaternary contact in the initial interaction of CD4 with the HIV-1 envelope trimer. 
  • Rad17 recruits the MRE11-RAD50-NBS1 complex to regulate the cellular response to DNA double-strand breaks. 
  • Rapid and orthogonal logic gating with a gibberellin-induced dimerization system. 
  • Reactivation of PTEN tumor suppressor for cancer treatment through inhibition of a MYC-WWP1 inhibitory pathway. 
  • Repletion of factor XIII following cardiopulmonary bypass using a recombinant A-subunit homodimer. A preliminary report. 
  • Revisiting the mystery of fibronectin multimers: the fibronectin matrix is composed of fibronectin dimers cross-linked by non-covalent bonds. 
  • Robust neutralizing antibodies elicited by HIV-1 JRFL envelope glycoprotein trimers in nonhuman primates. 
  • Sar1 assembly regulates membrane constriction and ER export. 
  • Septin Form and Function at the Cell Cortex. 
  • Septin filaments exhibit a dynamic, paired organization that is conserved from yeast to mammals. 
  • Sequence determinants of in cell condensate morphology, dynamics, and oligomerization as measured by number and brightness analysis. 
  • Sequential and Simultaneous Immunization of Rabbits with HIV-1 Envelope Glycoprotein SOSIP.664 Trimers from Clades A, B and C. 
  • SlmA forms a higher-order structure on DNA that inhibits cytokinetic Z-ring formation over the nucleoid. 
  • Soluble Envelope Glycoprotein Trimers from a CD4-Independent HIV-1 Elicit Antibody-Dependent Cellular Cytotoxicity-Mediating Antibodies in Guinea Pigs. 
  • Sparsely-sampled, high-resolution 4-D omit spectra for detection and assignment of intermolecular NOEs of protein complexes. 
  • Stabilization of the gp120 V3 loop through hydrophobic interactions reduces the immunodominant V3-directed non-neutralizing response to HIV-1 envelope trimers. 
  • Steric factors moderate conformational fluidity and contribute to the high proton sensitivity of Root effect hemoglobins. 
  • Structural Survey of Broadly Neutralizing Antibodies Targeting the HIV-1 Env Trimer Delineates Epitope Categories and Characteristics of Recognition. 
  • Structural and Biochemical Characterization of the Francisella tularensis Pathogenicity Regulator, Macrophage Locus Protein A (MglA). 
  • Structural basis for ADP-mediated transcriptional regulation by P1 and P7 ParA. 
  • Structural insight and characterization of human Twinkle helicase in mitochondrial disease. 
  • Structural mechanism of ATP-induced polymerization of the partition factor ParF: implications for DNA segregation. 
  • Structural mechanism of trimeric HIV-1 envelope glycoprotein activation. 
  • Structure and DNA-binding traits of the transition state regulator AbrB. 
  • Structure and accessibility of HA trimers on intact 2009 H1N1 pandemic influenza virus to stem region-specific neutralizing antibodies. 
  • Structure and immune recognition of trimeric pre-fusion HIV-1 Env. 
  • Structure and immunogenicity of a stabilized HIV-1 envelope trimer based on a group-M consensus sequence. 
  • Structure of a trimeric bacterial microcompartment shell protein, EtuB, associated with ethanol utilization in Clostridium kluyveri. 
  • Structure of the Z Ring-associated Protein, ZapD, Bound to the C-terminal Domain of the Tubulin-like Protein, FtsZ, Suggests Mechanism of Z Ring Stabilization through FtsZ Cross-linking. 
  • Structure-Based Design of a Soluble Prefusion-Closed HIV-1 Env Trimer with Reduced CD4 Affinity and Improved Immunogenicity. 
  • Structure-Based Stabilization of SOSIP Env Enhances Recombinant Ectodomain Durability and Yield. 
  • Structure-Function Analysis of Interallelic Complementation in ROOTY Transheterozygotes. 
  • Structure-based Design of Cyclically Permuted HIV-1 gp120 Trimers That Elicit Neutralizing Antibodies. 
  • Structures of AAA protein translocase Bcs1 suggest translocation mechanism of a folded protein. 
  • Structures of partition protein ParA with nonspecific DNA and ParB effector reveal molecular insights into principles governing Walker-box DNA segregation. 
  • Structures of the Bacillus subtilis glutamine synthetase dodecamer reveal large intersubunit catalytic conformational changes linked to a unique feedback inhibition mechanism. 
  • Structures of the Escherichia coli transcription activator and regulator of diauxie, XylR: an AraC DNA-binding family member with a LacI/GalR ligand-binding domain. 
  • Structures of the T. brucei kRNA editing factor MRB1590 reveal unique RNA-binding pore motif contained within an ABC-ATPase fold. 
  • Systematic solution to homo-oligomeric structures determined by NMR. 
  • Targeting HIV-1 envelope glycoprotein trimers to B cells by using APRIL improves antibody responses. 
  • The Functional Landscape of Patient-Derived RNF43 Mutations Predicts Sensitivity to Wnt Inhibition. 
  • The Glycan Hole Area of HIV-1 Envelope Trimers Contributes Prominently to the Induction of Autologous Neutralization. 
  • The cell division protein MinD from Pseudomonas aeruginosa dominates the assembly of the MinC-MinD copolymers. 
  • The mutagen and carcinogen cadmium is a high-affinity inhibitor of the zinc-dependent MutLα endonuclease. 
  • The structure of irisin reveals a novel intersubunit β-sheet fibronectin type III (FNIII) dimer: implications for receptor activation. 
  • Truncating CLCN1 mutations in myotonia congenita: variable patterns of inheritance. 
  • Two-Component Ferritin Nanoparticles for Multimerization of Diverse Trimeric Antigens. 
  • Tyrosine cross-linking reveals interfacial dynamics in adeno-associated viral capsids during infection. 
  • UNC-6 (netrin) stabilizes oscillatory clustering of the UNC-40 (DCC) receptor to orient polarity 
  • Upregulation of mTORC2 activation by the selective agonist of EPAC, 8-CPT-2Me-cAMP, in prostate cancer cells: assembly of a multiprotein signaling complex. 
  • Vaccine Induction of Heterologous Tier 2 HIV-1 Neutralizing Antibodies in Animal Models. 
  • ZDOCK server: interactive docking prediction of protein-protein complexes and symmetric multimers. 
• 

  Keywords of People

  • Bennett, Vann, George Barth Geller Distinguished Professor of Molecular Biology, Duke Cancer Institute
  • Erickson, Harold Paul, James B. Duke Distinguished Professor Emeritus, Cell Biology