Protein Stability

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  Subject Areas on Research

  • A Period2 Phosphoswitch Regulates and Temperature Compensates Circadian Period. 
  • A cellular genome-wide association study reveals human variation in microtubule stability and a role in inflammatory cell death. 
  • A common single nucleotide polymorphism A118G of the μ opioid receptor alters its N-glycosylation and protein stability. 
  • A miniaturized technique for assessing protein thermodynamics and function using fast determination of quantitative cysteine reactivity. 
  • ADA-SCID with 'WAZA-ARI' mutations that synergistically abolished ADA protein stability. 
  • Allosteric nanobodies reveal the dynamic range and diverse mechanisms of G-protein-coupled receptor activation. 
  • Analysis of Brain Protein Stability Changes in Mouse Models of Normal Aging and α-Synucleinopathy Reveals Age- and Disease-Related Differences. 
  • Are dust mite allergens more abundant and/or more stable than other Dermatophagoides pteronyssinus proteins? 
  • Autophagic activity dictates the cellular response to oncogenic RAS. 
  • Bazedoxifene exhibits antiestrogenic activity in animal models of tamoxifen-resistant breast cancer: implications for treatment of advanced disease. 
  • Both the conserved GRAS domain and nuclear localization are required for SHORT-ROOT movement. 
  • Characterization of the Saccharomyces cerevisiae ATP-Interactome using the iTRAQ-SPROX Technique. 
  • Cold sensitivity of the SARS-CoV-2 spike ectodomain. 
  • Comparative Analysis of Mass-Spectrometry-Based Proteomic Methods for Protein Target Discovery Using a One-Pot Approach. 
  • Conceptual Approaches to Modulating Antibody Effector Functions and Circulation Half-Life. 
  • Control of cellular GADD34 levels by the 26S proteasome. 
  • D614G Mutation Alters SARS-CoV-2 Spike Conformation and Enhances Protease Cleavage at the S1/S2 Junction. 
  • Design, display and immunogenicity of HIV1 gp120 fragment immunogens on virus-like particles. 
  • Developmental regulation of neural cell adhesion molecule in human prefrontal cortex. 
  • Direct observation of multimer stabilization in the mechanical unfolding pathway of a protein undergoing oligomerization. 
  • Discovery of Age-Related Protein Folding Stability Differences in the Mouse Brain Proteome. 
  • Discovery of Manassantin A Protein Targets Using Large-Scale Protein Folding and Stability Measurements. 
  • Discovery of Tamoxifen and N-Desmethyl Tamoxifen Protein Targets in MCF-7 Cells Using Large-Scale Protein Folding and Stability Measurements. 
  • Discovery of the Xenon-Protein Interactome Using Large-Scale Measurements of Protein Folding and Stability. 
  • Disruption of the HIV-1 Envelope allosteric network blocks CD4-induced rearrangements. 
  • Downregulation of the proapoptotic protein MOAP-1 by the UBR5 ubiquitin ligase and its role in ovarian cancer resistance to cisplatin. 
  • Energetics-based methods for protein folding and stability measurements. 
  • Enhanced cytoprotective effects of the inhibitor of apoptosis protein cellular IAP1 through stabilization with TRAF2. 
  • Environmental Stability of Plasmonic Biosensors Based on Natural versus Artificial Antibody. 
  • Fibronectin aggregation and assembly: the unfolding of the second fibronectin type III domain. 
  • First somatic mutation of E2F1 in a critical DNA binding residue discovered in well-differentiated papillary mesothelioma of the peritoneum. 
  • Framework Mutations of the 10-1074 bnAb Increase Conformational Stability, Manufacturability, and Stability While Preserving Full Neutralization Activity. 
  • G Protein and β-arrestin signaling bias at the ghrelin receptor. 
  • Global analysis of protein folding thermodynamics for disease state characterization. 
  • Haptoglobin alters oxygenation and oxidation of hemoglobin and decreases propagation of peroxide-induced oxidative reactions. 
  • Harnessing Environmental Ca2+ for Extracellular Protein Thermostabilization. 
  • Impact of vibrational entropy on the stability of unsolvated peptide helices with increasing length 
  • Insight into the mechanism of inactivation of ribonucleotide reductase by gemcitabine 5'-diphosphate in the presence or absence of reductant. 
  • Key mutations stabilize antigen-binding conformation during affinity maturation of a broadly neutralizing influenza antibody lineage. 
  • Large-Scale Analysis of Breast Cancer-Related Conformational Changes in Proteins Using Limited Proteolysis. 
  • Large-Scale Analysis of Breast Cancer-Related Conformational Changes in Proteins Using SILAC-SPROX. 
  • Mammalian Y chromosomes retain widely expressed dosage-sensitive regulators. 
  • Mapping and Engineering Functional Domains of the Assembly-Activating Protein of Adeno-associated Viruses. 
  • Mechanical Stability of a Small, Highly-Luminescent Engineered Protein NanoLuc. 
  • Mechanism of intracellular allosteric β2AR antagonist revealed by X-ray crystal structure. 
  • Microglassification™: a novel technique for protein dehydration. 
  • Molecular mechanism of activation of human musk receptors OR5AN1 and OR1A1 by (R)-muscone and diverse other musk-smelling compounds. 
  • Mst1/Mst2 regulate development and function of regulatory T cells through modulation of Foxo1/Foxo3 stability in autoimmune disease. 
  • Nanomechanics of streptavidin hubs for molecular materials. 
  • Nemo-like Kinase Drives Foxp3 Stability and Is Critical for Maintenance of Immune Tolerance by Regulatory T Cells. 
  • NuMA localization, stability, and function in spindle orientation involve 4.1 and Cdk1 interactions. 
  • Overcoming the solubility limit with solubility-enhancement tags: successful applications in biomolecular NMR studies. 
  • PGC-1 coactivators regulate MITF and the tanning response. 
  • Painting proteins with covalent labels: what's in the picture? 
  • Pathogenic Bacteria Target Plant Plasmodesmata to Colonize and Invade Surrounding Tissues. 
  • Picomole-scale characterization of protein stability and function by quantitative cysteine reactivity. 
  • Protein Folding Stability Changes Across the Proteome Reveal Targets of Cu Toxicity in E. coli . 
  • Proteome-Wide Characterization of Phosphorylation-Induced Conformational Changes in Breast Cancer. 
  • Proteome-Wide Structural Biology: An Emerging Field for the Structural Analysis of Proteins on the Proteomic Scale. 
  • Quantitation of protein-protein interactions by thermal stability shift analysis. 
  • Quaternary contact in the initial interaction of CD4 with the HIV-1 envelope trimer. 
  • SINAT E3 Ligases Control the Light-Mediated Stability of the Brassinosteroid-Activated Transcription Factor BES1 in Arabidopsis. 
  • Sel1L is indispensable for mammalian endoplasmic reticulum-associated degradation, endoplasmic reticulum homeostasis, and survival. 
  • Simultaneous determination of alpha-aminoadipic semialdehyde, piperideine-6-carboxylate and pipecolic acid by LC-MS/MS for pyridoxine-dependent seizures and folinic acid-responsive seizures. 
  • Site-specific phosphorylation of casein kinase 1 δ (CK1δ) regulates its activity towards the circadian regulator PER2. 
  • Spontaneous Unfolding-Refolding of Fibronectin Type III Domains Assayed by Thiol Exchange: THERMODYNAMIC STABILITY CORRELATES WITH RATES OF UNFOLDING RATHER THAN FOLDING. 
  • Stabilization of the gp120 V3 loop through hydrophobic interactions reduces the immunodominant V3-directed non-neutralizing response to HIV-1 envelope trimers. 
  • Structural and immunologic correlates of chemically stabilized HIV-1 envelope glycoproteins. 
  • Structural instability and divergence from conserved residues underlie intracellular retention of mammalian odorant receptors. 
  • Structure of active β-arrestin-1 bound to a G-protein-coupled receptor phosphopeptide. 
  • Structure-Based Design of a Soluble Prefusion-Closed HIV-1 Env Trimer with Reduced CD4 Affinity and Improved Immunogenicity. 
  • Temperature-induced opening of TRPV1 ion channel is stabilized by the pore domain. 
  • Temporal dynamics of Wnt-dependent transcriptome reveal an oncogenic Wnt/MYC/ribosome axis. 
  • The Chemistry of Lyophilized Blood Products. 
  • The LINK-A lncRNA activates normoxic HIF1α signalling in triple-negative breast cancer. 
  • The Streptomyces master regulator BldD binds c-di-GMP sequentially to create a functional BldD2-(c-di-GMP)4 complex. 
  • Thermodynamic analysis of ligand-induced changes in protein thermal unfolding applied to high-throughput determination of ligand affinities with extrinsic fluorescent dyes. 
  • Thermodynamic analysis of protein folding and stability using a tryptophan modification protocol. 
  • Thermodynamic analysis of protein-ligand binding interactions in complex biological mixtures using the stability of proteins from rates of oxidation. 
  • UNC-6 (netrin) stabilizes oscillatory clustering of the UNC-40 (DCC) receptor to orient polarity 
  • Use of a polyanionic carbomer, Carbopol971P, in combination with MF59, improves antibody responses to HIV-1 envelope glycoprotein. 
  • VEGF internalization is not required for VEGFR-2 phosphorylation in bioengineered surfaces with covalently linked VEGF 
  • Wnt proteins synergize to activate β-catenin signaling. 
  • Wnt/β-catenin pathway in podocytes integrates cell adhesion, differentiation, and survival. 
  • ZipA and FtsA* stabilize FtsZ-GDP miniring structures. 
  • β-arrestin1 mediates metastatic growth of breast cancer cells by facilitating HIF-1-dependent VEGF expression. 
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  Keywords of People

  • Bennett, Vann, George Barth Geller Distinguished Professor of Molecular Biology, Duke Cancer Institute
  • Erickson, Harold Paul, James B. Duke Distinguished Professor Emeritus, Cell Biology
  • McDonnell, Donald Patrick, Glaxo-Wellcome Distinguished Professor of Molecular Cancer Biology, in the School of Medicine, Cell Biology
  • Oas, Terrence Gilbert, Professor of Biochemistry, Biochemistry