Gene Knockdown Techniques

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  Subject Areas on Research

  • 58-kDa microspherule protein (MSP58) is novel Brahma-related gene 1 (BRG1)-associated protein that modulates p53/p21 senescence pathway. 
  • A Molecular Switch Regulating Cell Fate Choice between Muscle Progenitor Cells and Brown Adipocytes. 
  • A Multivariate Computational Method to Analyze High-Content RNAi Screening Data. 
  • A Small Molecule Targeting Mutagenic Translesion Synthesis Improves Chemotherapy. 
  • A conserved retromer-independent function for RAB-6.2 in C. elegans epidermis integrity. 
  • A functional genomics predictive network model identifies regulators of inflammatory bowel disease. 
  • A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. 
  • A novel missense mutation of Wilms' Tumor 1 causes autosomal dominant FSGS. 
  • A physical interaction network of dengue virus and human proteins. 
  • A protocol for heterologous expression and functional assay for mouse pheromone receptors. 
  • A systems genetics approach identifies Trp53inp2 as a link between cardiomyocyte glucose utilization and hypertrophic response. 
  • AHR2 knockdown prevents PAH-mediated cardiac toxicity and XRE- and ARE-associated gene induction in zebrafish (Danio rerio). 
  • AHR2 mediates cardiac teratogenesis of polycyclic aromatic hydrocarbons and PCB-126 in Atlantic killifish (Fundulus heteroclitus). 
  • AHR2 morpholino knockdown reduces the toxicity of total particulate matter to zebrafish embryos. 
  • AJAP1 expression modulates glioma cell motility and correlates with tumor growth and survival. 
  • Abl family kinases regulate endothelial barrier function in vitro and in mice. 
  • An heregulin-EGFR-HER3 autocrine signaling axis can mediate acquired lapatinib resistance in HER2+ breast cancer models. 
  • An oncogenic Ezh2 mutation induces tumors through global redistribution of histone 3 lysine 27 trimethylation. 
  • Animal model of Sar1b deficiency presents lipid absorption deficits similar to Anderson disease. 
  • Ankyrin-B interactions with spectrin and dynactin-4 are required for dystrophin-based protection of skeletal muscle from exercise injury. 
  • Ankyrin-G palmitoylation and βII-spectrin binding to phosphoinositide lipids drive lateral membrane assembly. 
  • Antioxidant responses and NRF2 in synergistic developmental toxicity of PAHs in zebrafish. 
  • Arhgap24 inactivates Rac1 in mouse podocytes, and a mutant form is associated with familial focal segmental glomerulosclerosis. 
  • Bcl-xL is an oncogenic driver in colorectal cancer. 
  • Blocking the interaction between apolipoprotein E and Aβ reduces intraneuronal accumulation of Aβ and inhibits synaptic degeneration. 
  • CARHSP1 is required for effective tumor necrosis factor alpha mRNA stabilization and localizes to processing bodies and exosomes. 
  • CBX4 Regulates Replicative Senescence of WI-38 Fibroblasts. 
  • CHOP mediates endoplasmic reticulum stress-induced apoptosis in Gimap5-deficient T cells. 
  • CRISPR Editing of Mutant IDH1 R132H Induces a CpG Methylation-Low State in Patient-Derived Glioma Models of G-CIMP. 
  • CRISPR/Cas9-Mediated BRCA1 Knockdown Adipose Stem Cells Promote Breast Cancer Progression. 
  • CYLD deubiquitinates RIP1 in the TNFα-induced necrosome to facilitate kinase activation and programmed necrosis. 
  • Calcineurin controls drug tolerance, hyphal growth, and virulence in Candida dubliniensis. 
  • Calcium influx through L-type CaV1.2 Ca2+ channels regulates mandibular development. 
  • Calreticulin chaperones regulate functional expression of vomeronasal type 2 pheromone receptors. 
  • Cardiomyopathy is associated with ribosomal protein gene haplo-insufficiency in Drosophila melanogaster. 
  • CircIRAK3 sponges miR-3607 to facilitate breast cancer metastasis. 
  • Clathrin light chains are required for the gyrating-clathrin recycling pathway and thereby promote cell migration. 
  • Coagulopathy in α-galactosyl transferase knockout pulmonary xenotransplants. 
  • Cyr61 mediates hepatocyte growth factor-dependent tumor cell growth, migration, and Akt activation. 
  • DICER1 deficit induces Alu RNA toxicity in age-related macular degeneration. 
  • DIO2 modifies inflammatory responses in chondrocytes. 
  • Derlin-1 is overexpressed in non-small cell lung cancer and promotes cancer cell invasion via EGFR-ERK-mediated up-regulation of MMP-2 and MMP-9. 
  • Dietary isothiocyanate-induced apoptosis via thiol modification of DNA topoisomerase IIα. 
  • Differential AMPK phosphorylation by glucagon and metformin regulates insulin signaling in human hepatic cells. 
  • Direct RNA sequencing reveals m6A modifications on adenovirus RNA are necessary for efficient splicing. 
  • Discovery of Genetic Variation on Chromosome 5q22 Associated with Mortality in Heart Failure. 
  • Disrupted junctional membrane complexes and hyperactive ryanodine receptors after acute junctophilin knockdown in mice. 
  • Disruption of the β1L Isoform of GABP Reverses Glioblastoma Replicative Immortality in a TERT Promoter Mutation-Dependent Manner. 
  • Dmrt1 induces the male pathway in a turtle species with temperature-dependent sex determination. 
  • Downregulation of the AU-rich RNA-binding protein ZFP36 in chronic HBV patients: implications for anti-inflammatory therapy. 
  • Drosophila Subdued is a moonlighting transmembrane protein 16 (TMEM16) that transports ions and phospholipids. 
  • EGFR-upregulated LIFR promotes SUCLG2-dependent castration resistance and neuroendocrine differentiation of prostate cancer. 
  • Efficient and specific gene knockdown by small interfering RNAs produced in bacteria. 
  • Efficient cross-priming of antiviral CD8+ T cells by antigen donor cells is GRP94 independent. 
  • Embryonic cardiotoxicity of weak aryl hydrocarbon receptor agonists and CYP1A inhibitor fluoranthene in the Atlantic killifish (Fundulus heteroclitus). 
  • Endothelial cell Pannexin1 modulates severity of ischemic stroke by regulating cerebral inflammation and myogenic tone. 
  • Engineering an analog-sensitive CDK12 cell line using CRISPR/Cas. 
  • Epithelial-mesenchymal transition in prostate cancer: providing new targets for therapy. 
  • Erk2 phosphorylation of Drp1 promotes mitochondrial fission and MAPK-driven tumor growth. 
  • Erlotinib-mediated inhibition of EGFR signaling induces metabolic oxidative stress through NOX4. 
  • Erythropoietin receptor contributes to melanoma cell survival in vivo. 
  • Fezf2 regulates telencephalic precursor differentiation from mouse embryonic stem cells. 
  • Full Spectrum of LPS Activation in Alveolar Macrophages of Healthy Volunteers by Whole Transcriptomic Profiling. 
  • Generation and characterization of a complete null estrogen receptor alpha mouse using Cre/LoxP technology. 
  • Genetic Engineering of Mesenchymal Stem Cells for Differential Matrix Deposition on 3D Woven Scaffolds. 
  • Genetic and bioinformatic analyses of the expression and function of PI3K regulatory subunit PIK3R3 in an Asian patient gastric cancer library. 
  • Genetic association for renal traits among participants of African ancestry reveals new loci for renal function. 
  • Genome-wide association and functional follow-up reveals new loci for kidney function. 
  • Genome-wide meta-analysis uncovers novel loci influencing circulating leptin levels. 
  • Global identification of MLL2-targeted loci reveals MLL2's role in diverse signaling pathways. 
  • Glutathione transferase pi class 2 (GSTp2) protects against the cardiac deformities caused by exposure to PAHs but not PCB-126 in zebrafish embryos. 
  • Gnao1 (G alphaO protein) is a likely genetic contributor to variation in physical dependence on opioids in mice. 
  • Gαi is required for carvedilol-induced β1 adrenergic receptor β-arrestin biased signaling. 
  • HIF-1 Alpha Regulates the Response of Primary Sarcomas to Radiation Therapy through a Cell Autonomous Mechanism. 
  • HOXB13 interaction with MEIS1 modifies proliferation and gene expression in prostate cancer. 
  • Hedgehog-GLI signaling inhibition suppresses tumor growth in squamous lung cancer. 
  • Hepatic Mitochondrial SAB Deletion or Knockdown Alleviates Diet-Induced Metabolic Syndrome, Steatohepatitis, and Hepatic Fibrosis. 
  • Hepatic insulin signaling is required for obesity-dependent expression of SREBP-1c mRNA but not for feeding-dependent expression. 
  • Hepatocyte nuclear factor-1β (HNF-1β) promotes glucose uptake and glycolytic activity in ovarian clear cell carcinoma. 
  • IKKβ promotes metabolic adaptation to glutamine deprivation via phosphorylation and inhibition of PFKFB3. 
  • IRG1 and Inducible Nitric Oxide Synthase Act Redundantly with Other Interferon-Gamma-Induced Factors To Restrict Intracellular Replication of Legionella pneumophila. 
  • Identification of prohibitin and prohibiton as novel factors binding to the p53 induced gene 3 (PIG3) promoter (TGYCC)(15) motif. 
  • In vivo Modeling Implicates APOL1 in Nephropathy: Evidence for Dominant Negative Effects and Epistasis under Anemic Stress. 
  • Inflammatory signaling sensitizes Piezo1 mechanotransduction in articular chondrocytes as a pathogenic feed-forward mechanism in osteoarthritis. 
  • Inhibition of Human Dendritic Cell ER Stress Response Reduces T Cell Alloreactivity Yet Spares Donor Anti-tumor Immunity. 
  • Inhibition of Spleen Tyrosine Kinase Potentiates Paclitaxel-Induced Cytotoxicity in Ovarian Cancer Cells by Stabilizing Microtubules. 
  • Integrating genetic, transcriptional, and functional analyses to identify 5 novel genes for atrial fibrillation. 
  • Integrin alpha 6 regulates glioblastoma stem cells. 
  • Intrastromal Gene Therapy Prevents and Reverses Advanced Corneal Clouding in a Canine Model of Mucopolysaccharidosis I. 
  • Intrinsic Antiviral Activity of Optineurin Prevents Hyperproliferation of a Primary Herpes Simplex Virus Type 2 Infection. 
  • Knockdown of AHR1A but not AHR1B exacerbates PAH and PCB-126 toxicity in zebrafish (Danio rerio) embryos. 
  • Knockdown of the cell cycle inhibitor p21 enhances cartilage formation by induced pluripotent stem cells. 
  • Kv2.1 Clustering Contributes to Insulin Exocytosis and Rescues Human β-Cell Dysfunction. 
  • LGR5 is associated with tumor aggressiveness in papillary thyroid cancer. 
  • LRRK2 and Rab10 coordinate macropinocytosis to mediate immunological responses in phagocytes. 
  • Ligation of cell surface GRP78 with antibody directed against the COOH-terminal domain of GRP78 suppresses Ras/MAPK and PI 3-kinase/AKT signaling while promoting caspase activation in human prostate cancer cells. 
  • Live Imaging of Host-Parasite Interactions in a Zebrafish Infection Model Reveals Cryptococcal Determinants of Virulence and Central Nervous System Invasion. 
  • Loss of ARID1A in Tumor Cells Renders Selective Vulnerability to Combined Ionizing Radiation and PARP Inhibitor Therapy. 
  • MOV10 Provides Antiviral Activity against RNA Viruses by Enhancing RIG-I-MAVS-Independent IFN Induction. 
  • Manganese superoxide dismutase expression in endothelial progenitor cells accelerates wound healing in diabetic mice. 
  • Manganese superoxide dismutase-mediated inside-out signaling in HaCaT human keratinocytes and SKH-1 mouse skin. 
  • Manifold roles of β-arrestins in GPCR signaling elucidated with siRNA and CRISPR/Cas9. 
  • Mechanism of threshold size assessment: Metamorphosis is triggered by the TGF-beta/Activin ligand Myoglianin. 
  • Melanoma immunotherapy using mature DCs expressing the constitutive proteasome. 
  • Mitochondrial Superoxide Dismutase Has a Protumorigenic Role in Ovarian Clear Cell Carcinoma. 
  • Monoclonal antibody targeting of N-cadherin inhibits prostate cancer growth, metastasis and castration resistance. 
  • Mutations in the gene that encodes the F-actin binding protein anillin cause FSGS. 
  • N-cadherin is Key to Expression of the Nucleus Pulposus Cell Phenotype under Selective Substrate Culture Conditions. 
  • N6-methyladenosine alters RNA structure to regulate binding of a low-complexity protein. 
  • Neuron-specific Sumo1-3 knockdown in mice impairs episodic and fear memories. 
  • New loci for body fat percentage reveal link between adiposity and cardiometabolic disease risk. 
  • Nkx2-5 Second Heart Field Target Gene Ccdc117 Regulates DNA Metabolism and Proliferation. 
  • Notch3 pathway alterations in ovarian cancer. 
  • Nuclear factor erythroid-derived factor 2-related factor 2 regulates transcription of CCAAT/enhancer-binding protein β during adipogenesis. 
  • Nuclear lamin stiffness is a barrier to 3D migration, but softness can limit survival. 
  • Otx but not Mitf transcription factors are required for zebrafish retinal pigment epithelium development. 
  • Overlapping high-resolution copy number alterations in cancer genomes identified putative cancer genes in hepatocellular carcinoma. 
  • PIP5K1α promotes myogenic differentiation via AKT activation and calcium release. 
  • PORCN moonlights in a Wnt-independent pathway that regulates cancer cell proliferation. 
  • Pathway for lipid A biosynthesis in Arabidopsis thaliana resembling that of Escherichia coli. 
  • Perturbations to the hedgehog pathway in sea urchin embryos. 
  • Physiologic hypoxia promotes maintenance of CML stem cells despite effective BCR-ABL1 inhibition. 
  • Primary cilia and the reciprocal activation of AKT and SMAD2/3 regulate stretch-induced autophagy in trabecular meshwork cells. 
  • Probing the role of PPARγ in the regulation of late-onset Alzheimer's disease-associated genes. 
  • Production of highly potent recombinant siRNAs in Escherichia coli. 
  • Protein Arginine Methyltransferase 5 Promotes pICln-Dependent Androgen Receptor Transcription in Castration-Resistant Prostate Cancer. 
  • Proteomic analysis of mitotic RNA polymerase II reveals novel interactors and association with proteins dysfunctional in disease. 
  • Quantitative analysis of the chromatin proteome in disease reveals remodeling principles and identifies high mobility group protein B2 as a regulator of hypertrophic growth. 
  • RIP1-dependent and independent effects of necrostatin-1 in necrosis and T cell activation. 
  • Recurrent WNT pathway alterations are frequent in relapsed small cell lung cancer. 
  • Regulation of early adipose commitment by Zfp521. 
  • Rem2-targeted shRNAs reduce frequency of miniature excitatory postsynaptic currents without altering voltage-gated Ca²⁺ currents. 
  • SENP3-mediated host defense response contains HBV replication and restores protein synthesis. 
  • SINAT E3 Ligases Control the Light-Mediated Stability of the Brassinosteroid-Activated Transcription Factor BES1 in Arabidopsis. 
  • SOCS1, SOCS3, and PIAS1 promote myogenic differentiation by inhibiting the leukemia inhibitory factor-induced JAK1/STAT1/STAT3 pathway. 
  • Selective coexpression of VEGF receptor 2 in EGFRvIII-positive glioblastoma cells prevents cellular senescence and contributes to their aggressive nature. 
  • Serotonin synthesis, release and reuptake in terminals: a mathematical model. 
  • Signal Transducer and Activator of Transcription 3, Mediated Remodeling of the Tumor Microenvironment Results in Enhanced Tumor Drug Delivery in a Mouse Model of Pancreatic Cancer. 
  • Simvastatin inhibits IFN regulatory factor 4 expression and Th17 cell differentiation in CD4+ T cells derived from patients with multiple sclerosis. 
  • Smac mimetic Birinapant induces apoptosis and enhances TRAIL potency in inflammatory breast cancer cells in an IAP-dependent and TNF-α-independent mechanism. 
  • Small nucleolar RNAs U32a, U33, and U35a are critical mediators of metabolic stress. 
  • Somatic deletions of genes regulating MSH2 protein stability cause DNA mismatch repair deficiency and drug resistance in human leukemia cells. 
  • Stbd1 is highly elevated in skeletal muscle of Pompe disease mice but suppression of its expression does not affect lysosomal glycogen accumulation. 
  • Structural basis of lipid binding for the membrane-embedded tetraacyldisaccharide-1-phosphate 4'-kinase LpxK. 
  • Systems proteomics of cardiac chromatin identifies nucleolin as a regulator of growth and cellular plasticity in cardiomyocytes. 
  • TLR3 deficiency impairs spinal cord synaptic transmission, central sensitization, and pruritus in mice. 
  • TRPV4 is a regulator of adipose oxidative metabolism, inflammation, and energy homeostasis. 
  • Tafenoquine: A Step toward Malaria Elimination. 
  • Targeting GIPC/synectin in pancreatic cancer inhibits tumor growth. 
  • Targeting RET Kinase in Neuroendocrine Prostate Cancer. 
  • Targeting UDP-α-D-glucose 6-dehydrogenase inhibits glioblastoma growth and migration. 
  • Targeting VE-PTP phosphatase protects the kidney from diabetic injury. 
  • Targeting inhibitors of the tumor suppressor PP2A for the treatment of pancreatic cancer. 
  • The ABL2 kinase regulates an HSF1-dependent transcriptional program required for lung adenocarcinoma brain metastasis. 
  • The CUL7/F-box and WD repeat domain containing 8 (CUL7/Fbxw8) ubiquitin ligase promotes degradation of hematopoietic progenitor kinase 1. 
  • The DEK oncoprotein is a Su(var) that is essential to heterochromatin integrity. 
  • The GNA13-RhoA signaling axis suppresses expression of tumor protective Kallikreins. 
  • The bacterial effector HopM1 suppresses PAMP-triggered oxidative burst and stomatal immunity. 
  • The combination of TPL2 knockdown and TNFα causes synthetic lethality via caspase-8 activation in human carcinoma cell lines. 
  • The deubiquitinases USP33 and USP20 coordinate beta2 adrenergic receptor recycling and resensitization. 
  • The effect of charge-reversal amphiphile spacer composition on DNA and siRNA delivery. 
  • The effects of PPARγ on the regulation of the TOMM40-APOE-C1 genes cluster. 
  • The feelgood mutation in zebrafish dysregulates COPII-dependent secretion of select extracellular matrix proteins in skeletal morphogenesis. 
  • The histone demethylase KDM6B regulates temperature-dependent sex determination in a turtle species. 
  • The long noncoding RNA Chaer defines an epigenetic checkpoint in cardiac hypertrophy. 
  • The potential repertoire of the innate immune system in the bladder: expression of pattern recognition receptors in the rat bladder and a rat urothelial cell line (MYP3 cells). 
  • The proximity-labeling technique BioID identifies sorting nexin 6 as a member of the insulin-like growth factor 1 (IGF1)-IGF1 receptor pathway. 
  • The regulation of ferroptosis by MESH1 through the activation of the integrative stress response. 
  • The role of WNT10B in normal prostate gland development and prostate cancer. 
  • The therapeutic effects of SET/I2PP2A inhibitors on canine melanoma. 
  • The transcription factor Twist1 in the distal nephron but not in macrophages propagates aristolochic acid nephropathy. 
  • The vacuolar-ATPase modulates matrix metalloproteinase isoforms in human pancreatic cancer. 
  • Therapeutic targeting of erbB3 with MM-121/SAR256212 enhances antitumor activity of paclitaxel against erbB2-overexpressing breast cancer. 
  • Thermosensory and nonthermosensory isoforms of Drosophila melanogaster TRPA1 reveal heat-sensor domains of a thermoTRP Channel. 
  • Three sodium channel mutations from Aedes albopictus confer resistance to Type I, but not Type II pyrethroids. 
  • Thyroid hormone stimulates hepatic lipid catabolism via activation of autophagy. 
  • Toward understanding cancer stem cell heterogeneity in the tumor microenvironment. 
  • Type 2 Diabetes Variants Disrupt Function of SLC16A11 through Two Distinct Mechanisms. 
  • Ubiquitin fold modifier 1 (UFM1) and its target UFBP1 protect pancreatic beta cells from ER stress-induced apoptosis. 
  • Ubiquitin-binding site 2 of ataxin-3 prevents its proteasomal degradation by interacting with Rad23. 
  • Viral Replication Complexes Are Targeted by LC3-Guided Interferon-Inducible GTPases. 
  • Wnt-regulated lncRNA discovery enhanced by in vivo identification and CRISPRi functional validation. 
  • Wnt6 activates endoderm in the sea urchin gene regulatory network. 
  • X-linked inhibitor of apoptosis protein mediates tumor cell resistance to antibody-dependent cellular cytotoxicity. 
  • Yin Yang 1 plays an essential role in breast cancer and negatively regulates p27. 
  • Zebrafish cardiotoxicity: the effects of CYP1A inhibition and AHR2 knockdown following exposure to weak aryl hydrocarbon receptor agonists. 
  • αB-crystallin: a novel regulator of breast cancer metastasis to the brain. 
  • β-Arrestin-2 desensitizes the transient receptor potential vanilloid 1 (TRPV1) channel. 
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  Keywords of People

  • McLendon, Roger Edwin, Professor of Pathology, Pathology
  • Pitt, Geoffrey Stuart, Adjunct Professor in the Department of Medicine, Medicine, Cardiology