Gene Knockout Techniques

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  Subject Areas on Research

  • A CRISPR Screen Identifies the Cell Polarity Determinant Crumbs 3 as an Adeno-associated Virus Restriction Factor in Hepatocytes. 
  • A Missing Link to Vitamin B12 Metabolism. 
  • A Protocol for the Production of Integrase-deficient Lentiviral Vectors for CRISPR/Cas9-mediated Gene Knockout in Dividing Cells. 
  • A Rapidly Evolving Polybasic Motif Modulates Bacterial Detection by Guanylate Binding Proteins. 
  • A conserved role of αA-crystallin in the development of the zebrafish embryonic lens. 
  • A dual-target molecular mechanism of pyrethrum repellency against mosquitoes. 
  • A flucytosine-responsive Mbp1/Swi4-like protein, Mbs1, plays pleiotropic roles in antifungal drug resistance, stress response, and virulence of Cryptococcus neoformans. 
  • A thioesterase bypasses the requirement for exogenous fatty acids in the plsX deletion of Streptococcus pneumoniae. 
  • ATM Paradoxically Promotes Oncogenic Transformation via Transcriptional Reprogramming. 
  • Abi1 loss drives prostate tumorigenesis through activation of EMT and non-canonical WNT signaling. 
  • Activation of Piezo1 mechanosensitive ion channel in HEK293T cells by 30 MHz vertically deployed surface acoustic waves. 
  • Altered lymphopoiesis and immunodeficiency in miR-142 null mice. 
  • Alternative Splicing of Rice WRKY62 and WRKY76 Transcription Factor Genes in Pathogen Defense. 
  • An inner activation gate controls TMEM16F phospholipid scrambling. 
  • Axl2 integrates polarity establishment, maintenance, and environmental stress response in the filamentous fungus Ashbya gossypii. 
  • Brain microvascular injury and white matter disease provoked by diabetes-associated hyperamylinemia. 
  • CERKL gene knockout disturbs photoreceptor outer segment phagocytosis and causes rod-cone dystrophy in zebrafish. 
  • Calcium/calmodulin-dependent protein kinase kinase 2 regulates macrophage-mediated inflammatory responses. 
  • Caspase-3 regulates the migration, invasion and metastasis of colon cancer cells. 
  • Cell-cell adhesions and cell contractility are upregulated upon desmosome disruption. 
  • Coding and non-coding roles of MOCCI (C15ORF48) coordinate to regulate host inflammation and immunity. 
  • Conditional knockout of Fgf13 in murine hearts increases arrhythmia susceptibility and reveals novel ion channel modulatory roles. 
  • Copper Influences the Antibacterial Outcomes of a β-Lactamase-Activated Prochelator against Drug-Resistant Bacteria. 
  • DOK2 inhibits EGFR-mutated lung adenocarcinoma. 
  • Defective innate immunity and hyperinflammation in newborn cystic fibrosis transmembrane conductance regulator-knockout ferret lungs. 
  • Deletion of CaMKK2 from the liver lowers blood glucose and improves whole-body glucose tolerance in the mouse. 
  • Dengue Virus Targets Nrf2 for NS2B3-Mediated Degradation Leading to Enhanced Oxidative Stress and Viral Replication. 
  • Differential Control of iNKT Cell Effector Lineage Differentiation by the Forkhead Box Protein O1 (Foxo1) Transcription Factor. 
  • Differential LRRK2 expression in the cortex, striatum, and substantia nigra in transgenic and nontransgenic rodents. 
  • Dihydrophenylalanine: a prephenate-derived Photorhabdus luminescens antibiotic and intermediate in dihydrostilbene biosynthesis. 
  • Direct RNA sequencing reveals m6A modifications on adenovirus RNA are necessary for efficient splicing. 
  • Distinct Responses of Stem Cells to Telomere Uncapping-A Potential Strategy to Improve the Safety of Cell Therapy. 
  • Distinct function of estrogen receptor α in smooth muscle and fibroblast cells in prostate development. 
  • Distinct regulatory mechanisms governing embryonic versus adult adipocyte maturation. 
  • Effects of the sazetidine-a family of compounds on the body temperature in wildtype, nicotinic receptor β2-/- and α7-/- mice. 
  • Endogenous Retrovirus Activation as a Key Mechanism of Anti-Tumor Immune Response in Radiotherapy. 
  • Fission yeast Cyk3p is a transglutaminase-like protein that participates in cytokinesis and cell morphogenesis. 
  • Functional characterization of a Nudix hydrolase AtNUDX8 upon pathogen attack indicates a positive role in plant immune responses. 
  • Gangliosides are essential endosomal receptors for quasi-enveloped and naked hepatitis A virus. 
  • Heme Oxygenase-1/Carbon Monoxide System and Embryonic Stem Cell Differentiation and Maturation into Cardiomyocytes. 
  • Hepatitis C Virus Infection Is Inhibited by a Noncanonical Antiviral Signaling Pathway Targeted by NS3-NS4A. 
  • Hepatitis-C-virus-induced microRNAs dampen interferon-mediated antiviral signaling. 
  • High XRCC1 protein expression is associated with poorer survival in patients with head and neck squamous cell carcinoma. 
  • Histone lysine methyltransferase SDG8 is involved in brassinosteroid-regulated gene expression in Arabidopsis thaliana. 
  • Human UDP-galactose 4'-epimerase (GALE) is required for cell-surface glycome structure and function. 
  • Humoral Reactivity of Renal Transplant-Waitlisted Patients to Cells From GGTA1/CMAH/B4GalNT2, and SLA Class I Knockout Pigs. 
  • Id3 restricts the developmental potential of gamma delta lineage during thymopoiesis. 
  • Identification of a Locus Near ULK1 Associated With Progression-Free Survival in Ovarian Cancer. 
  • Importance of the Conserved Carboxyl-Terminal CNOT1 Binding Domain to Tristetraprolin Activity In Vivo. 
  • In Vivo Role of INPP4B in Tumor and Metastasis Suppression through Regulation of PI3K-AKT Signaling at Endosomes. 
  • Incorporation of extracellular fatty acids by a fatty acid kinase-dependent pathway in Staphylococcus aureus. 
  • Introducing MX Cassettes into Saccharomyces cerevisiae. 
  • Isocitrate-to-SENP1 signaling amplifies insulin secretion and rescues dysfunctional β cells. 
  • JMJD6 cleaves MePCE to release positive transcription elongation factor b (P-TEFb) in higher eukaryotes. 
  • Key roles of necroptotic factors in promoting tumor growth. 
  • Knocking out lca5 in zebrafish causes cone-rod dystrophy due to impaired outer segment protein trafficking. 
  • Knockout of Nr2e3 prevents rod photoreceptor differentiation and leads to selective L-/M-cone photoreceptor degeneration in zebrafish. 
  • Loss of aryl hydrocarbon receptor potentiates FoxM1 signaling to enhance self-renewal of colonic stem and progenitor cells. 
  • MAP4K3 mediates amino acid-dependent regulation of autophagy via phosphorylation of TFEB. 
  • MX Cassettes for Knocking Out Genes in Yeast. 
  • Metabolic regulation of protein N-alpha-acetylation by Bcl-xL promotes cell survival. 
  • Mice Lacking RIP3 Kinase are not Protected from Acute Radiation Syndrome. 
  • Microcephaly with altered cortical layering in GIT1 deficiency revealed by quantitative neuroimaging. 
  • Mitochondrial quality-control dysregulation in conditional HO-1-/- mice. 
  • Modulation of PICALM Levels Perturbs Cellular Cholesterol Homeostasis. 
  • Mutation of Nogo-B receptor, a subunit of cis-prenyltransferase, causes a congenital disorder of glycosylation. 
  • NADPH Oxidase 4 Regulates Inflammation in Ischemic Heart Failure: Role of Soluble Epoxide Hydrolase. 
  • Niche-specific contribution to streptococcal virulence of a MalR-regulated carbohydrate binding protein. 
  • Ocular mucosal CD11b+ and CD103+ mouse dendritic cells under normal conditions and in allergic immune responses. 
  • Optimal pulse length of insonification for Piezo1 activation and intracellular calcium response. 
  • Pathway for lipid A biosynthesis in Arabidopsis thaliana resembling that of Escherichia coli. 
  • Physical interaction between VIVID and white collar complex regulates photoadaptation in Neurospora. 
  • Popping Out MX Cassettes from Saccharomyces cerevisiae. 
  • Probe Sensitivity to Cortical versus Intracellular Cytoskeletal Network Stiffness. 
  • RNase L Is Involved in Liposaccharide-Induced Lung Inflammation. 
  • Regulation of mammalian autophagy by class II and III PI 3-kinases through PI3P synthesis. 
  • Role of Cryptococcus neoformans Rho1 GTPases in the PKC1 signaling pathway in response to thermal stress. 
  • Role of the DSC1 channel in regulating neuronal excitability in Drosophila melanogaster: extending nervous system stability under stress. 
  • Silencing the porcine iGb3s gene does not affect Galα3Gal levels or measures of anticipated pig-to-human and pig-to-primate acute rejection. 
  • Statistical analysis of genetic interactions in Tn-Seq data. 
  • Steroid-sensitive nephrotic syndrome candidate gene CLVS1 regulates podocyte oxidative stress and endocytosis. 
  • Systematic Dissection of the Metabolic-Apoptotic Interface in AML Reveals Heme Biosynthesis to Be a Regulator of Drug Sensitivity. 
  • T-Scan: A Genome-wide Method for the Systematic Discovery of T Cell Epitopes. 
  • TCF1 links GIPR signaling to the control of beta cell function and survival. 
  • The Arp2/3 complex has essential roles in vesicle trafficking and transcytosis in the mammalian small intestine. 
  • The Primary Effect on the Proteome of ARID1A-mutated Ovarian Clear Cell Carcinoma is Downregulation of the Mevalonate Pathway at the Post-transcriptional Level. 
  • The arginase inhibitor Nω-hydroxy-nor-arginine (nor-NOHA) induces apoptosis in leukemic cells specifically under hypoxic conditions but CRISPR/Cas9 excludes arginase 2 (ARG2) as the functional target. 
  • The characterization of twenty sequenced human genomes. 
  • The desirable donor pig to eliminate all xenoreactive antigens. 
  • The development of CD4 binding site antibodies during HIV-1 infection. 
  • The fate of human platelets exposed to porcine renal endothelium: a single-pass model of platelet uptake in domestic and genetically modified porcine organs. 
  • Transcriptional network predicts viral set point during acute HIV-1 infection. 
  • Variation in the fitness effects of mutations with population density and size in Escherichia coli. 
  • Wnt/β-catenin pathway in podocytes integrates cell adhesion, differentiation, and survival.