High-Throughput Screening Assays

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  Subject Areas on Research

  • A CRISPR Activation Screen Identifies a Pan-avian Influenza Virus Inhibitory Host Factor. 
  • A Multivariate Computational Method to Analyze High-Content RNAi Screening Data. 
  • A Precision Medicine Drug Discovery Pipeline Identifies Combined CDK2 and 9 Inhibition as a Novel Therapeutic Strategy in Colorectal Cancer. 
  • A biomimetic Schlemm's canal inner wall: A model to study outflow physiology, glaucoma pathology and high-throughput drug screening. 
  • A densely interconnected genome-wide network of microRNAs and oncogenic pathways revealed using gene expression signatures. 
  • A high throughput screen identifies Nefopam as targeting cell proliferation in β-catenin driven neoplastic and reactive fibroproliferative disorders. 
  • A high-throughput histoarray for quantitative molecular profiling of multiple, uniformly oriented medaka (Oryzias latipes) embryos. 
  • A high-throughput in vitro drug screen in a genetically engineered mouse model of diffuse intrinsic pontine glioma identifies BMS-754807 as a promising therapeutic agent. 
  • A high-throughput neutralizing antibody assay for COVID-19 diagnosis and vaccine evaluation. 
  • A new class of temporarily phenotypic enhancers identified by CRISPR/Cas9-mediated genetic screening. 
  • A rapid and affordable screening platform for membrane protein trafficking. 
  • A review of three-dimensional in vitro tissue models for drug discovery and transport studies. 
  • A whole animal chemical screen approach to identify modifiers of intestinal neutrophilic inflammation. 
  • Acoustic separation of circulating tumor cells. 
  • Allosteric "beta-blocker" isolated from a DNA-encoded small molecule library. 
  • Assessment of genetic and nongenetic interactions for the prediction of depressive symptomatology: an analysis of the Wisconsin Longitudinal Study using machine learning algorithms. 
  • Bayesian Multi-Plate High-Throughput Screening of Compounds. 
  • Bisphenol A affects androgen receptor function via multiple mechanisms. 
  • Characterization of Plasmodium liver stage inhibition by halofuginone. 
  • Circulating Tumor Cell Phenotyping via High-Throughput Acoustic Separation. 
  • Circulating tumor cell-derived organoids: Current challenges and promises in medical research and precision medicine. 
  • Clopidogrel metaboliser status based on point-of-care CYP2C19 genetic testing in patients with coronary artery disease. 
  • CometChip enables parallel analysis of multiple DNA repair activities. 
  • Comparison of gene expression profiles induced by coarse, fine, and ultrafine particulate matter. 
  • Computationally guided high-throughput design of self-assembling drug nanoparticles. 
  • DNA methylation and miRNA expression profiling in childhood B-cell acute lymphoblastic leukemia. 
  • Derivatives of plant phenolic compound affect the type III secretion system of Pseudomonas aeruginosa via a GacS-GacA two-component signal transduction system. 
  • Designing a Better Mousetrap: Reflections on the November 28, 2017, US Food and Drug Administration Meeting on Next-Generation "High-Sensitivity" Cardiac Troponin Assays to Diagnose Myocardial Infarction. 
  • Developing High-Throughput Assays to Analyze and Screen Electrophysiological Phenotypes. 
  • Development and application of a multiplex assay for the simultaneous measurement of antibody responses elicited by common childhood vaccines. 
  • Development and implementation of a proficiency testing program for Luminex bead-based cytokine assays. 
  • Development of a Fluorescence-Based, High-Throughput SARS-CoV-2 3CLpro Reporter Assay. 
  • Development of a cell-based, high-throughput screening assay for ATM kinase inhibitors. 
  • Development of a high-throughput screening assay for inhibitors of small ubiquitin-like modifier proteases. 
  • Development of a precision medicine pipeline to identify personalized treatments for colorectal cancer. 
  • Development of small molecules targeting the Wnt pathway for the treatment of colon cancer: a high-throughput screening approach. 
  • Digital microfluidics comes of age: high-throughput screening to bedside diagnostic testing for genetic disorders in newborns. 
  • First quantitative high-throughput screen in zebrafish identifies novel pathways for increasing pancreatic β-cell mass. 
  • Fluorescence linked enzyme chemoproteomic strategy for discovery of a potent and selective DAPK1 and ZIPK inhibitor. 
  • Fluorescent indicator displacement assays to identify and characterize small molecule interactions with RNA. 
  • Genetic Analysis of Complex Traits in Saccharomyces cerevisiae . 
  • Genetic Dissection of Heritable Traits in Yeast Using Bulk Segregant Analysis. 
  • HTS-Compatible CometChip Enables Genetic Screening for Modulators of Apoptosis and DNA Double-Strand Break Repair. 
  • High spatial resolution proteomic comparison of the brain in humans and chimpanzees. 
  • High-Throughput Computational Search for Half-Metallic Oxides. 
  • High-Throughput Mapping of B Cell Receptor Sequences to Antigen Specificity. 
  • High-Throughput Tissue Bioenergetics Analysis Reveals Identical Metabolic Allometric Scaling for Teleost Hearts and Whole Organisms. 
  • High-performance virtual screening by targeting a high-resolution RNA dynamic ensemble. 
  • High-throughput cardiac safety evaluation and multi-parameter arrhythmia profiling of cardiomyocytes using microelectrode arrays. 
  • High-throughput flow cytometry screening reveals a role for junctional adhesion molecule a as a cancer stem cell maintenance factor. 
  • High-throughput identification and dendritic cell-based functional validation of MHC class I-restricted Mycobacterium tuberculosis epitopes. 
  • High-throughput phenotyping of multicellular organisms: finding the link between genotype and phenotype. 
  • High-throughput quantitative analysis of HIV-1 and SIV-specific ADCC-mediating antibody responses. 
  • High-throughput screening for modulators of cellular contractile force. 
  • High-throughput targeted proteomics discovery approach and spontaneous reperfusion in ST-segment elevation myocardial infarction. 
  • Human genome-wide measurement of drug-responsive regulatory activity. 
  • Identification of Autophagy-Inhibiting Factors of Mycobacterium tuberculosis by High-Throughput Loss-of-Function Screening. 
  • Identification of Novel Mast Cell Activators Using Cell-Based High-Throughput Screening. 
  • In vivo monitoring of cardiomyocyte proliferation to identify chemical modifiers of heart regeneration. 
  • Inflammatory breast cancer tumor emboli express high levels of anti-apoptotic proteins: use of a quantitative high content and high-throughput 3D IBC spheroid assay to identify targeting strategies. 
  • Introduction to the special issue on emerging high throughput and complementary model screens for neurotoxicology. 
  • LabKey Server NAb: a tool for analyzing, visualizing and sharing results from neutralizing antibody assays. 
  • Live-cell Measurement of Odorant Receptor Activation Using a Real-time cAMP Assay. 
  • Loss-of-Function RNAi Screen to Identify Necrosis-Signaling Molecules. 
  • Methods for high-dimensional analysis of cells dissociated from cryopreserved synovial tissue 
  • Mitochondrial Diagnostics: A Multiplexed Assay Platform for Comprehensive Assessment of Mitochondrial Energy Fluxes. 
  • Multiplex newborn screening for Pompe, Fabry, Hunter, Gaucher, and Hurler diseases using a digital microfluidic platform. 
  • Nuclear morphology measurements with angle-resolved low coherence interferometry for application to cell biology and early cancer detection. 
  • Optimization and validation of a neutralizing antibody assay for HIV-1 in A3R5 cells. 
  • Optimization and validation of the TZM-bl assay for standardized assessments of neutralizing antibodies against HIV-1. 
  • Overcoming redundancy: an RNAi enhancer screen for morphogenesis genes in Caenorhabditis elegans. 
  • Performance of a new, rapid, automated immunoassay for the detection of anti-platelet factor 4/heparin complex antibodies. 
  • Pilot and feasibility study: prospective proteomic profiling of mammary epithelial cells from high-risk women provides evidence of activation of pro-survival pathways. 
  • Predicting protein-ligand interactions based on bow-pharmacological space and Bayesian additive regression trees. 
  • Protein-DNA binding in the absence of specific base-pair recognition. 
  • Quantitative high-throughput efficacy profiling of approved oncology drugs in inflammatory breast cancer models of acquired drug resistance and re-sensitization. 
  • Rac1/Pak1/p38/MMP-2 Axis Regulates Angiogenesis in Ovarian Cancer. 
  • Robust, linear correlations between growth rates and β-lactam-mediated lysis rates. 
  • Scaffold Hopping and Optimization of Maleimide Based Porcupine Inhibitors. 
  • Screening Regulatory Element Function with CRISPR/Cas9-based Epigenome Editing. 
  • Serologic screening of United States blood donors for Babesia microti using an investigational enzyme immunoassay. 
  • Single-cell microarray enables high-throughput evaluation of DNA double-strand breaks and DNA repair inhibitors. 
  • Targeting RNA with small molecules: from fundamental principles towards the clinic. 
  • The Phenix software for automated determination of macromolecular structures. 
  • Tissue-engineered blood vessels as promising tools for testing drug toxicity. 
  • Transcriptional regulation improves the throughput of three-hybrid counter selections in Saccharomyces cerevisiae. 
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  Keywords of People

  • Calakos, Nicole, Lincoln Financial Group Distinguished Professor of Neurobiology, Duke Science & Society
  • Ferrari, Guido, Professor in Surgery, Molecular Genetics and Microbiology
  • McLendon, Roger Edwin, Professor of Pathology, Pathology
  • Weinhold, Kent James, Joseph W. and Dorothy W. Beard Distinguished Professor of Experimental Surgery, in the School of Medicine, Immunology