High-Throughput Screening Assays

• 

  Subject Areas on Research

  • A CRISPR Activation Screen Identifies a Pan-avian Influenza Virus Inhibitory Host Factor. 
  • A Multivariate Computational Method to Analyze High-Content RNAi Screening Data. 
  • A biomimetic Schlemm's canal inner wall: A model to study outflow physiology, glaucoma pathology and high-throughput drug screening. 
  • A comprehensive approach to identifying repurposed drugs to treat SCN8A epilepsy. 
  • A densely interconnected genome-wide network of microRNAs and oncogenic pathways revealed using gene expression signatures. 
  • A high throughput screen identifies Nefopam as targeting cell proliferation in β-catenin driven neoplastic and reactive fibroproliferative disorders. 
  • A high-throughput histoarray for quantitative molecular profiling of multiple, uniformly oriented medaka (Oryzias latipes) embryos. 
  • A high-throughput in vitro drug screen in a genetically engineered mouse model of diffuse intrinsic pontine glioma identifies BMS-754807 as a promising therapeutic agent. 
  • A new class of temporarily phenotypic enhancers identified by CRISPR/Cas9-mediated genetic screening. 
  • A rapid and affordable screening platform for membrane protein trafficking. 
  • A review of three-dimensional in vitro tissue models for drug discovery and transport studies. 
  • A whole animal chemical screen approach to identify modifiers of intestinal neutrophilic inflammation. 
  • Acoustic separation of circulating tumor cells. 
  • Allosteric "beta-blocker" isolated from a DNA-encoded small molecule library. 
  • An extractive removal step optimized for a high-throughput α-cellulose extraction method for δ13C and δ18O stable isotope ratio analysis in conifer tree rings. 
  • Assessment of genetic and nongenetic interactions for the prediction of depressive symptomatology: an analysis of the Wisconsin Longitudinal Study using machine learning algorithms. 
  • Bisphenol A affects androgen receptor function via multiple mechanisms. 
  • Characterization of Plasmodium liver stage inhibition by halofuginone. 
  • Clopidogrel metaboliser status based on point-of-care CYP2C19 genetic testing in patients with coronary artery disease. 
  • Comparison of gene expression profiles induced by coarse, fine, and ultrafine particulate matter. 
  • Composite primary neuronal high-content screening assay for Huntington's disease incorporating non-cell-autonomous interactions. 
  • Derivatives of plant phenolic compound affect the type III secretion system of Pseudomonas aeruginosa via a GacS-GacA two-component signal transduction system. 
  • Designing a Better Mousetrap: Reflections on the November 28, 2017, US Food and Drug Administration Meeting on Next-Generation "High-Sensitivity" Cardiac Troponin Assays to Diagnose Myocardial Infarction. 
  • Developing High-Throughput Assays to Analyze and Screen Electrophysiological Phenotypes. 
  • Development and application of a multiplex assay for the simultaneous measurement of antibody responses elicited by common childhood vaccines. 
  • Development and implementation of a proficiency testing program for Luminex bead-based cytokine assays. 
  • Development of a cell-based, high-throughput screening assay for ATM kinase inhibitors. 
  • Development of a high-throughput screening assay for inhibitors of small ubiquitin-like modifier proteases. 
  • Development of small molecules targeting the Wnt pathway for the treatment of colon cancer: a high-throughput screening approach. 
  • Digital microfluidics comes of age: high-throughput screening to bedside diagnostic testing for genetic disorders in newborns. 
  • Fluorescence linked enzyme chemoproteomic strategy for discovery of a potent and selective DAPK1 and ZIPK inhibitor. 
  • Genetic Analysis of Complex Traits in Saccharomyces cerevisiae. 
  • Genetic Dissection of Heritable Traits in Yeast Using Bulk Segregant Analysis. 
  • High spatial resolution proteomic comparison of the brain in humans and chimpanzees. 
  • High-Throughput Tissue Bioenergetics Analysis Reveals Identical Metabolic Allometric Scaling for Teleost Hearts and Whole Organisms. 
  • High-performance virtual screening by targeting a high-resolution RNA dynamic ensemble. 
  • High-throughput cardiac safety evaluation and multi-parameter arrhythmia profiling of cardiomyocytes using microelectrode arrays. 
  • High-throughput flow cytometry screening reveals a role for junctional adhesion molecule a as a cancer stem cell maintenance factor. 
  • High-throughput identification and dendritic cell-based functional validation of MHC class I-restricted Mycobacterium tuberculosis epitopes. 
  • High-throughput phenotyping of multicellular organisms: finding the link between genotype and phenotype. 
  • High-throughput quantitative analysis of HIV-1 and SIV-specific ADCC-mediating antibody responses. 
  • High-throughput screening for modulators of cellular contractile force. 
  • High-throughput screening of microchip-synthesized genes in programmable double-emulsion droplets. 
  • Human genome-wide measurement of drug-responsive regulatory activity. 
  • In vivo monitoring of cardiomyocyte proliferation to identify chemical modifiers of heart regeneration. 
  • Inflammatory breast cancer tumor emboli express high levels of anti-apoptotic proteins: use of a quantitative high content and high-throughput 3D IBC spheroid assay to identify targeting strategies. 
  • Introduction to the special issue on emerging high throughput and complementary model screens for neurotoxicology. 
  • LabKey Server NAb: a tool for analyzing, visualizing and sharing results from neutralizing antibody assays. 
  • Live-cell Measurement of Odorant Receptor Activation Using a Real-time cAMP Assay. 
  • Loss-of-Function RNAi Screen to Identify Necrosis-Signaling Molecules. 
  • Microfluidic synthesis of multifunctional Janus particles for biomedical applications. 
  • Multiplex newborn screening for Pompe, Fabry, Hunter, Gaucher, and Hurler diseases using a digital microfluidic platform. 
  • Nuclear morphology measurements with angle-resolved low coherence interferometry for application to cell biology and early cancer detection. 
  • Optimization and validation of a neutralizing antibody assay for HIV-1 in A3R5 cells. 
  • Optimization and validation of the TZM-bl assay for standardized assessments of neutralizing antibodies against HIV-1. 
  • Overcoming redundancy: an RNAi enhancer screen for morphogenesis genes in Caenorhabditis elegans. 
  • Performance of a new, rapid, automated immunoassay for the detection of anti-platelet factor 4/heparin complex antibodies. 
  • Pilot and feasibility study: prospective proteomic profiling of mammary epithelial cells from high-risk women provides evidence of activation of pro-survival pathways. 
  • Protein-DNA binding in the absence of specific base-pair recognition. 
  • Quantitative high-throughput efficacy profiling of approved oncology drugs in inflammatory breast cancer models of acquired drug resistance and re-sensitization. 
  • Rac1/Pak1/p38/MMP-2 Axis Regulates Angiogenesis in Ovarian Cancer. 
  • Recognition of decay accelerating factor and alpha(v)beta(3) by inactivated hantaviruses: Toward the development of high-throughput screening flow cytometry assays. 
  • Robust, linear correlations between growth rates and β-lactam-mediated lysis rates. 
  • Scaffold Hopping and Optimization of Maleimide Based Porcupine Inhibitors. 
  • Screening Regulatory Element Function with CRISPR/Cas9-based Epigenome Editing. 
  • Selective mechanism-based inactivation of CYP3A4 by CYP3cide (PF-04981517) and its utility as an in vitro tool for delineating the relative roles of CYP3A4 versus CYP3A5 in the metabolism of drugs. 
  • Single-cell microarray enables high-throughput evaluation of DNA double-strand breaks and DNA repair inhibitors. 
  • The Phenix software for automated determination of macromolecular structures. 
  • Tissue-engineered blood vessels as promising tools for testing drug toxicity. 
  • Towards new business models for R&D for novel antibiotics. 
  • Use of cartilage derived from murine induced pluripotent stem cells for osteoarthritis drug screening. 
• 

  Keywords of People

  • Calakos, Nicole, Professor in Neurology, Duke Science & Society
  • Ferrari, Guido, Associate Professor of Surgery, Molecular Genetics and Microbiology
  • McLendon, Roger Edwin, Professor of Pathology, Pathology
  • Weinhold, Kent James, Joseph W. and Dorothy W. Beard Professor of Experimental Surgery, in the School of Medicine, Immunology