Primary Cell Culture

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  Subject Areas on Research

  • A genetically defined disease model reveals that urothelial cells can initiate divergent bladder cancer phenotypes. 
  • ANK2 autism mutation targeting giant ankyrin-B promotes axon branching and ectopic connectivity. 
  • Abl family kinases regulate FcγR-mediated phagocytosis in murine macrophages. 
  • Acetalated Dextran Microparticles for Codelivery of STING and TLR7/8 Agonists. 
  • Adenylosuccinate Is an Insulin Secretagogue Derived from Glucose-Induced Purine Metabolism. 
  • Amenable epigenetic traits of dental pulp stem cells underlie high capability of xeno-free episomal reprogramming. 
  • Apolipoprotein E4 Impairs Neuronal Insulin Signaling by Trapping Insulin Receptor in the Endosomes. 
  • Asteropsin A: an unusual cystine-crosslinked peptide from porifera enhances neuronal Ca2+ influx. 
  • Biochemical Pathways and Myometrial Cell Differentiation Leading to Nodule Formation Containing Collagen and Fibronectin. 
  • CRISPR/Cas9-Mediated BRCA1 Knockdown Adipose Stem Cells Promote Breast Cancer Progression. 
  • Cellular pharmacokinetic and pharmacodynamic analyses of ethacrynic acid: Implications in topical drug delivery in the eye. 
  • Clinical Trial in a Dish: Personalized Stem Cell-Derived Cardiomyocyte Assay Compared With Clinical Trial Results for Two QT-Prolonging Drugs. 
  • Coding and non-coding roles of MOCCI (C15ORF48) coordinate to regulate host inflammation and immunity. 
  • Conditions that promote primary human skeletal myoblast culture and muscle differentiation in vitro. 
  • Continuous Culture of Mouse Primary B Lymphocytes by Forced Expression of Bach2. 
  • Deletion of CaMKK2 from the liver lowers blood glucose and improves whole-body glucose tolerance in the mouse. 
  • Differential DNA methylation patterns in human Schlemm's canal endothelial cells with glaucoma. 
  • Differential Killing of Salmonella enterica Serovar Typhi by Antibodies Targeting Vi and Lipopolysaccharide O:9 Antigen. 
  • Directional Exosome Proteomes Reflect Polarity-Specific Functions in Retinal Pigmented Epithelium Monolayers. 
  • Disruption of the β1L Isoform of GABP Reverses Glioblastoma Replicative Immortality in a TERT Promoter Mutation-Dependent Manner. 
  • Diverse neurotoxicants target the differentiation of embryonic neural stem cells into neuronal and glial phenotypes. 
  • Dlg5 regulates dendritic spine formation and synaptogenesis by controlling subcellular N-cadherin localization. 
  • Dysregulation of mitochondrial bioenergetics and quality control by HIV-1 Tat in cardiomyocytes. 
  • Endothelial colony forming cells (ECFCs) as a model for studying effects of low-dose ionizing radiation: growth inhibition by a single dose. 
  • Enhanced HIF2α expression during human trophoblast differentiation into syncytiotrophoblast suppresses transcription of placental growth factor. 
  • Erythrocyte plasma membrane-bound ERK1/2 activation promotes ICAM-4-mediated sickle red cell adhesion to endothelium. 
  • Fibroblast growth factor 23 directly targets hepatocytes to promote inflammation in chronic kidney disease. 
  • Fibrotic Changes to Schlemm's Canal Endothelial Cells in Glaucoma. 
  • Fluorescence linked enzyme chemoproteomic strategy for discovery of a potent and selective DAPK1 and ZIPK inhibitor. 
  • Global phosphotyrosinylated protein profile of cell-matrix adhesion complexes of trabecular meshwork cells. 
  • Guanylate binding proteins enable rapid activation of canonical and noncanonical inflammasomes in Chlamydia-infected macrophages. 
  • HASF (C3orf58) is a novel ligand of the insulin-like growth factor 1 receptor. 
  • Hepatic Mitochondrial SAB Deletion or Knockdown Alleviates Diet-Induced Metabolic Syndrome, Steatohepatitis, and Hepatic Fibrosis. 
  • IL-6/STAT3 promotes regeneration of airway ciliated cells from basal stem cells. 
  • In Vitro Pre-Clinical Validation of Suicide Gene Modified Anti-CD33 Redirected Chimeric Antigen Receptor T-Cells for Acute Myeloid Leukemia. 
  • In vitro and in vivo inhibition of malaria parasite infection by monoclonal antibodies against Plasmodium falciparum circumsporozoite protein (CSP). 
  • In vitro determinants of asbestos fiber toxicity: effect on the relative toxicity of Libby amphibole in primary human airway epithelial cells. 
  • Increased BCR responsiveness in B cells from patients with chronic GVHD. 
  • Inflammatory signaling sensitizes Piezo1 mechanotransduction in articular chondrocytes as a pathogenic feed-forward mechanism in osteoarthritis. 
  • Inhibition of LRRK2 kinase activity promotes anterograde axonal transport and presynaptic targeting of α-synuclein. 
  • Interferon lambda protects the female reproductive tract against Zika virus infection. 
  • Isolation and Culture of Murine Primary Chondrocytes: Costal and Growth Plate Cartilage. 
  • Isolation and culture of murine primary chondrocytes. 
  • KIF18B is a cell type-specific regulator of spindle orientation in the epidermis. 
  • Keratin Profiling by Single-Cell RNA-Sequencing Identifies Human Prostate Stem Cell Lineage Hierarchy and Cancer Stem-Like Cells. 
  • Lowering circulating apolipoprotein E levels improves aged bone fracture healing. 
  • M. tuberculosis-Initiated Human Mannose Receptor Signaling Regulates Macrophage Recognition and Vesicle Trafficking by FcRγ-Chain, Grb2, and SHP-1. 
  • MKK3 influences mitophagy and is involved in cigarette smoke-induced inflammation. 
  • MYH9 E1841K Mutation Augments Proteinuria and Podocyte Injury and Migration. 
  • Macrophage and adipocyte interaction as a source of inflammation in kidney disease. 
  • Macroporous Dual-compartment Hydrogels for Minimally Invasive Transplantation of Primary Human Hepatocytes. 
  • Mechano-growth factor peptide, the COOH terminus of unprocessed insulin-like growth factor 1, has no apparent effect on myoblasts or primary muscle stem cells. 
  • Mitochondrial protein adducts formation and mitochondrial dysfunction during N-acetyl-m-aminophenol (AMAP)-induced hepatotoxicity in primary human hepatocytes. 
  • Moderate hypothermia induces marked increase in levels and nuclear accumulation of SUMO2/3-conjugated proteins in neurons. 
  • Modulation of Human Macrophage Responses to Mycobacterium tuberculosis by Silver Nanoparticles of Different Size and Surface Modification. 
  • Mutational signatures of de-differentiation in functional non-coding regions of melanoma genomes. 
  • Mutations Preventing Regulated Exon Skipping in MET Cause Osteofibrous Dysplasia. 
  • Nuclear factor erythroid-derived factor 2-related factor 2 regulates transcription of CCAAT/enhancer-binding protein β during adipogenesis. 
  • Open conformers of HLA-F are high-affinity ligands of the activating NK-cell receptor KIR3DS1. 
  • PD-L1 inhibits acute and chronic pain by suppressing nociceptive neuron activity via PD-1. 
  • PI3K regulation of RAC1 is required for KRAS-induced pancreatic tumorigenesis in mice. 
  • Perivascular dendritic cells elicit anaphylaxis by relaying allergens to mast cells via microvesicles. 
  • Piezo1 is a mechanically activated ion channel and mediates pressure induced pancreatitis. 
  • Primary cilia and the reciprocal activation of AKT and SMAD2/3 regulate stretch-induced autophagy in trabecular meshwork cells. 
  • Progesterone, Inflammatory Cytokine (TNF-α), and Oxidative Stress (H2O2) Regulate Progesterone Receptor Membrane Component 1 Expression in Fetal Membrane Cells. 
  • Proteomic Analysis of Primary Human Airway Epithelial Cells Exposed to the Respiratory Toxicant Diacetyl. 
  • RasGRP1 is a potential biomarker to stratify anti-EGFR therapy response in colorectal cancer. 
  • Redox-Active Mn Porphyrin-based Potent SOD Mimic, MnTnBuOE-2-PyP(5+), Enhances Carbenoxolone-Mediated TRAIL-Induced Apoptosis in Glioblastoma Multiforme. 
  • Role of MCP-1 and IL-8 in viral anterior uveitis, and contractility and fibrogenic activity of trabecular meshwork cells. 
  • Small molecule dual-inhibitors of TRPV4 and TRPA1 for attenuation of inflammation and pain. 
  • Sodium channel NaV1.3 is important for enterochromaffin cell excitability and serotonin release. 
  • Somatodendritic Expression of JAM2 Inhibits Oligodendrocyte Myelination. 
  • Stat3 binds to mtDNA and regulates mitochondrial gene expression in keratinocytes. 
  • Synaptotagmin 17 controls neurite outgrowth and synaptic physiology via distinct cellular pathways. 
  • Targeting In Vivo Metabolic Vulnerabilities of Th2 and Th17 Cells Reduces Airway Inflammation. 
  • The Importance of IL-6 in the Development of LAT-Mediated Autoimmunity. 
  • The Ubiquitin-Modifying Enzyme A20 Terminates C-Type Lectin Receptor Signals and Is a Suppressor of Host Defense against Systemic Fungal Infection. 
  • The arginase inhibitor Nω-hydroxy-nor-arginine (nor-NOHA) induces apoptosis in leukemic cells specifically under hypoxic conditions but CRISPR/Cas9 excludes arginase 2 (ARG2) as the functional target. 
  • The comprehensive role of E-cadherin in maintaining prostatic epithelial integrity during oncogenic transformation and tumor progression. 
  • The copper chelator ATN-224 induces peroxynitrite-dependent cell death in hematological malignancies. 
  • The expression level of C19MC miRNAs in early pregnancy and in response to viral infection. 
  • The transcription factor Twist1 in the distal nephron but not in macrophages propagates aristolochic acid nephropathy. 
  • The ubiquitin conjugating enzyme Ube2W regulates solubility of the Huntington's disease protein, huntingtin. 
  • Tracing Self-Reactive B Cells in Normal Mice. 
  • Transfection efficiency and transgene expression kinetics of mRNA delivered in naked and nanoparticle format. 
  • Tropism of SARS-CoV-2 for human cortical astrocytes. 
  • WIPI1 is a conserved mediator of right ventricular failure. 
  • Wild-type Kras expands and exhausts hematopoietic stem cells. 
  • cAMP-Response Element-Binding 3-Like Protein 1 (CREB3L1) is Required for Decidualization and its Expression is Decreased in Women with Endometriosis. 
  • ΔN-TRPV1: A Molecular Co-detector of Body Temperature and Osmotic Stress. 
  • β-Arrestin-2 desensitizes the transient receptor potential vanilloid 1 (TRPV1) channel. 
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  Keywords of People

  • Zennadi, Rahima, Associate Professor in Medicine, Pathology