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Down-regulation of IL-2 receptor alpha (CD25) characterizes human gammadelta-T cells rendered resistant to apoptosis after CD2 engagement in the presence of IL-12.

Publication ,  Journal Article
Guo, BL; Hollmig, KA; Lopez, RD
Published in: Cancer Immunol Immunother
January 2002

We recently identified a CD2-mediated, IL-12-dependent signaling pathway that inhibits apoptosis in mitogen-stimulated human gammadelta-T cells. Here we show that gammadelta-T cells which acquire resistance to mitogen-induced apoptosis upregulate IL-12 receptor beta 1 subunit (IL-12Rbeta1); in contrast, gammadelta-T cells which remain sensitive to mitogen-induced apoptosis fail to express IL-12Rbeta1. Next we show that gammadelta-T cells which are rendered resistant to mitogen-induced apoptosis attenuate their expression of the IL-2 receptor alpha chain (IL-2Ralpha/CD25), this in part accounting for their acquired resistance to IL-2-induced death. In contrast, apoptosis-sensitive gammadelta-T cells are shown to persist in their expression of IL-2Ralpha/CD25, thus remaining sensitive to IL-2-induced death. Moreover, we show that apoptosis-resistant, but not apoptosis-sensitive, gammadelta-T cells display an enhanced responsiveness to IL-15, a finding in keeping with the known function of IL-15 as a growth and survival factor. Finally, we present evidence to suggest that this differential responsiveness to IL-15 occurs in part by the increased expression of the IL-15Ralpha chain on apoptosis-resistant gammadelta-T cells, compared to apoptosis-sensitive gammadelta-T cells. The biological and clinical implications of these findings are discussed.

Duke Scholars

Published In

Cancer Immunol Immunother

DOI

ISSN

0340-7004

Publication Date

January 2002

Volume

50

Issue

11

Start / End Page

625 / 637

Location

Germany

Related Subject Headings

  • T-Lymphocytes
  • Receptors, Interleukin-2
  • Receptors, Antigen, T-Cell, gamma-delta
  • Interleukin-12
  • Immunology
  • Humans
  • Flow Cytometry
  • Down-Regulation
  • Cells, Cultured
  • CD2 Antigens
 

Citation

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ICMJE
MLA
NLM
Guo, B. L., Hollmig, K. A., & Lopez, R. D. (2002). Down-regulation of IL-2 receptor alpha (CD25) characterizes human gammadelta-T cells rendered resistant to apoptosis after CD2 engagement in the presence of IL-12. Cancer Immunol Immunother, 50(11), 625–637. https://doi.org/10.1007/s00262-001-0244-4
Guo, Ben L., Klaus A. Hollmig, and Richard D. Lopez. “Down-regulation of IL-2 receptor alpha (CD25) characterizes human gammadelta-T cells rendered resistant to apoptosis after CD2 engagement in the presence of IL-12.Cancer Immunol Immunother 50, no. 11 (January 2002): 625–37. https://doi.org/10.1007/s00262-001-0244-4.
Guo, Ben L., et al. “Down-regulation of IL-2 receptor alpha (CD25) characterizes human gammadelta-T cells rendered resistant to apoptosis after CD2 engagement in the presence of IL-12.Cancer Immunol Immunother, vol. 50, no. 11, Jan. 2002, pp. 625–37. Pubmed, doi:10.1007/s00262-001-0244-4.
Journal cover image

Published In

Cancer Immunol Immunother

DOI

ISSN

0340-7004

Publication Date

January 2002

Volume

50

Issue

11

Start / End Page

625 / 637

Location

Germany

Related Subject Headings

  • T-Lymphocytes
  • Receptors, Interleukin-2
  • Receptors, Antigen, T-Cell, gamma-delta
  • Interleukin-12
  • Immunology
  • Humans
  • Flow Cytometry
  • Down-Regulation
  • Cells, Cultured
  • CD2 Antigens