CD58/LFA-3 and IL-12 provided by activated monocytes are critical in the in vitro expansion of CD56+ T cells.
Journal Article (Journal Article)
A small proportion of human CD3+ T lymphocytes are known to co-express CD56, an antigen usually restricted in its expression to natural killer (NK) cells. Whereas the in vivo function of CD3+ CD56+ T cells remains unknown, we and others have previously shown that both in vitro and in vivo, these cells can mediate a significantly greater degree of MHC-unrestricted cytotoxicitv against a variety of human tumor cells when compared to either CD3+ CD56- T cells or lymphokine activated killer (LAK) cells. While the mechanismns regulating the in vivo expansion of CD56+ T cells are not known, here we demonstrate the importance of CD2-mediated IL-12-dependent signals in the in vitro expansion of CD56+ T cells. Specifically, we show that activated monocytes provide a contact dependent factor (CD58/LFA-3) and a soluble factor (IL-12), both critical for the in vitro expansion of CD56+ T cells. The biological and therapeutic implications of these findings are discussed.
Full Text
Duke Authors
Cited Authors
- Lopez, RD; Waller, EK; Lu, PH; Negrin, RS
Published Date
- February 2001
Published In
Volume / Issue
- 49 / 12
Start / End Page
- 629 - 640
PubMed ID
- 11258789
International Standard Serial Number (ISSN)
- 0340-7004
Digital Object Identifier (DOI)
- 10.1007/s002620000148
Language
- eng
Conference Location
- Germany