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CD58/LFA-3 and IL-12 provided by activated monocytes are critical in the in vitro expansion of CD56+ T cells.

Publication ,  Journal Article
Lopez, RD; Waller, EK; Lu, PH; Negrin, RS
Published in: Cancer Immunol Immunother
February 2001

A small proportion of human CD3+ T lymphocytes are known to co-express CD56, an antigen usually restricted in its expression to natural killer (NK) cells. Whereas the in vivo function of CD3+ CD56+ T cells remains unknown, we and others have previously shown that both in vitro and in vivo, these cells can mediate a significantly greater degree of MHC-unrestricted cytotoxicitv against a variety of human tumor cells when compared to either CD3+ CD56- T cells or lymphokine activated killer (LAK) cells. While the mechanismns regulating the in vivo expansion of CD56+ T cells are not known, here we demonstrate the importance of CD2-mediated IL-12-dependent signals in the in vitro expansion of CD56+ T cells. Specifically, we show that activated monocytes provide a contact dependent factor (CD58/LFA-3) and a soluble factor (IL-12), both critical for the in vitro expansion of CD56+ T cells. The biological and therapeutic implications of these findings are discussed.

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Published In

Cancer Immunol Immunother

DOI

ISSN

0340-7004

Publication Date

February 2001

Volume

49

Issue

12

Start / End Page

629 / 640

Location

Germany

Related Subject Headings

  • T-Lymphocytes
  • Monocytes
  • Interleukin-12
  • Interferon-gamma
  • In Vitro Techniques
  • Immunology
  • Humans
  • Gene Expression
  • Flow Cytometry
  • Enzyme-Linked Immunosorbent Assay
 

Citation

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Lopez, R. D., Waller, E. K., Lu, P. H., & Negrin, R. S. (2001). CD58/LFA-3 and IL-12 provided by activated monocytes are critical in the in vitro expansion of CD56+ T cells. Cancer Immunol Immunother, 49(12), 629–640. https://doi.org/10.1007/s002620000148
Lopez, R. D., E. K. Waller, P. H. Lu, and R. S. Negrin. “CD58/LFA-3 and IL-12 provided by activated monocytes are critical in the in vitro expansion of CD56+ T cells.Cancer Immunol Immunother 49, no. 12 (February 2001): 629–40. https://doi.org/10.1007/s002620000148.
Lopez RD, Waller EK, Lu PH, Negrin RS. CD58/LFA-3 and IL-12 provided by activated monocytes are critical in the in vitro expansion of CD56+ T cells. Cancer Immunol Immunother. 2001 Feb;49(12):629–40.
Lopez, R. D., et al. “CD58/LFA-3 and IL-12 provided by activated monocytes are critical in the in vitro expansion of CD56+ T cells.Cancer Immunol Immunother, vol. 49, no. 12, Feb. 2001, pp. 629–40. Pubmed, doi:10.1007/s002620000148.
Lopez RD, Waller EK, Lu PH, Negrin RS. CD58/LFA-3 and IL-12 provided by activated monocytes are critical in the in vitro expansion of CD56+ T cells. Cancer Immunol Immunother. 2001 Feb;49(12):629–640.
Journal cover image

Published In

Cancer Immunol Immunother

DOI

ISSN

0340-7004

Publication Date

February 2001

Volume

49

Issue

12

Start / End Page

629 / 640

Location

Germany

Related Subject Headings

  • T-Lymphocytes
  • Monocytes
  • Interleukin-12
  • Interferon-gamma
  • In Vitro Techniques
  • Immunology
  • Humans
  • Gene Expression
  • Flow Cytometry
  • Enzyme-Linked Immunosorbent Assay