Skip to main content
Journal cover image

Characterization of the interleukin 1 receptor-associated kinase 4 (IRAK4)-encoding gene in salmonid fish: the functional copy is rearranged in Oncorhynchus mykiss and that factor can impair TLR signaling in mammalian cells.

Publication ,  Journal Article
Brietzke, A; Goldammer, T; Rebl, H; Korytář, T; Köllner, B; Yang, W; Rebl, A; Seyfert, H-M
Published in: Fish Shellfish Immunol
January 2014

The interleukin 1 receptor-associated kinase 4 (IRAK4) is an essential factor for TLR-mediated activation of the host's immune functions subsequent to pathogen contact. We have characterized the respective cDNA and gene sequences from three salmonid species, salmon, rainbow trout and maraena whitefish. The gene from salmon is structured into eleven exons, as is the mammalian homologue, while exons have been fused in the genes from the two other salmonid species. Rainbow trout expresses also a pseudogene at low levels. Its basic structure resembles more closely the primordial gene than the functional copy does. The N-terminal death domain and the C-terminal protein kinase domain of the factors are better conserved throughout evolution than the linker domain. The deduced amino acid sequences of the factors from all three species group together in an evolutionary tree of IRAK4 factors. Scrutinizing expression and function of IRAK4 from rainbow trout, we found its highest expression in head kidney and spleen and lowest expression in muscle tissue. Infecting fish with Aeromonas salmonicida did not modulate its expression during 72 h of observation. Expression of a GFP-tagged trout IRAK4 revealed, expectedly, its cytoplasmic localization in human HEK-293 cells. However, this factor significantly quenched in a dose-dependent fashion not only the pathogen-induced stimulation of NF-κB factors in the HEK-293 reconstitution system of TLR2 signaling, but also the basal NF-κB levels in unstimulated control cells. Our data unexpectedly imply that IRAK4 is involved in establishing threshold levels of active NF-κB in resting cells.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Fish Shellfish Immunol

DOI

EISSN

1095-9947

Publication Date

January 2014

Volume

36

Issue

1

Start / End Page

206 / 214

Location

England

Related Subject Headings

  • Toll-Like Receptors
  • Signal Transduction
  • Sequence Analysis, DNA
  • Sequence Alignment
  • Salmonidae
  • Reverse Transcriptase Polymerase Chain Reaction
  • RNA, Messenger
  • Phylogeny
  • Molecular Sequence Data
  • Interleukin-1 Receptor-Associated Kinases
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Brietzke, A., Goldammer, T., Rebl, H., Korytář, T., Köllner, B., Yang, W., … Seyfert, H.-M. (2014). Characterization of the interleukin 1 receptor-associated kinase 4 (IRAK4)-encoding gene in salmonid fish: the functional copy is rearranged in Oncorhynchus mykiss and that factor can impair TLR signaling in mammalian cells. Fish Shellfish Immunol, 36(1), 206–214. https://doi.org/10.1016/j.fsi.2013.11.005
Brietzke, Andreas, Tom Goldammer, Henrike Rebl, Tomáš Korytář, Bernd Köllner, Wei Yang, Alexander Rebl, and Hans-Martin Seyfert. “Characterization of the interleukin 1 receptor-associated kinase 4 (IRAK4)-encoding gene in salmonid fish: the functional copy is rearranged in Oncorhynchus mykiss and that factor can impair TLR signaling in mammalian cells.Fish Shellfish Immunol 36, no. 1 (January 2014): 206–14. https://doi.org/10.1016/j.fsi.2013.11.005.
Journal cover image

Published In

Fish Shellfish Immunol

DOI

EISSN

1095-9947

Publication Date

January 2014

Volume

36

Issue

1

Start / End Page

206 / 214

Location

England

Related Subject Headings

  • Toll-Like Receptors
  • Signal Transduction
  • Sequence Analysis, DNA
  • Sequence Alignment
  • Salmonidae
  • Reverse Transcriptase Polymerase Chain Reaction
  • RNA, Messenger
  • Phylogeny
  • Molecular Sequence Data
  • Interleukin-1 Receptor-Associated Kinases