Salmonella typhimurium impedes innate immunity with a mast-cell-suppressing protein tyrosine phosphatase, SptP.
Journal Article (Journal Article)
The virulence of Salmonella is linked to its invasive capacity and suppression of adaptive immunity. This does not explain, however, the rapid dissemination of the pathogen after it breaches the gut. In our study, S. Typhimurium suppressed degranulation of local mast cells (MCs), resulting in limited neutrophil recruitment and restricting outflow of vascular contents into infection sites, thus facilitating bacterial spread. MC suppression was mediated by secreted effector protein (SptP), which shares structural homology with Yersinia YopH. SptP functioned by dephosphorylating the vesicle fusion protein N-ethylmalemide-sensitive factor and by blocking phosphorylation of Syk. Without SptP, orally challenged S. Typhimurium failed to suppress MC degranulation and exhibited limited colonization of the mesenteric lymph nodes. Administration of SptP to sites of E. coli infection markedly enhanced its virulence. Thus, SptP-mediated inactivation of local MCs is a powerful mechanism utilized by S. Typhimurium to impede early innate immunity.
Full Text
Duke Authors
Cited Authors
- Choi, HW; Brooking-Dixon, R; Neupane, S; Lee, C-J; Miao, EA; Staats, HF; Abraham, SN
Published Date
- December 12, 2013
Published In
Volume / Issue
- 39 / 6
Start / End Page
- 1108 - 1120
PubMed ID
- 24332031
Pubmed Central ID
- PMC4326046
Electronic International Standard Serial Number (EISSN)
- 1097-4180
Digital Object Identifier (DOI)
- 10.1016/j.immuni.2013.11.009
Language
- eng
Conference Location
- United States