Corneal Angiogenesis Assay
New vessel growth in the avascular and transparent cornea occurs under a variety of pathological conditions and is readily distinguishable. Therefore, the corneal neovascularization (CNV) assay has become a widely used in vivo model for angiogenesis research. Many techniques, including chemical cauterization and mechanical manipulations have been developed. This chapter describes the background, physiology, induction techniques and image analysis of CNV with emphasis on the most frequently applied micropocket assay. In this assay, angiogenesis is induced by placing a polymer pellet containing an angiogenic factor, such as bFGF, into a surgically created stromal pocket and allowing for its sustained release. A putative anti- or pro-angiogenic substance is incorporated into the pellet, or given locally or systemically. The degree of suppression or enhancement of angiogenesis is quantified by image analysis. CNV assay offers a quantitable and precise means of screening for angiogenesis inhibitors, but also has limitations. It is desirable to combine CNV with other in vivo assays to investigate angiogenesis. © 2006 John Wiley & Sons, Ltd.
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