Cell-specific aptamers for targeted therapies.

Published

Journal Article (Review)

Many signalling proteins involved in diverse functions such as cell growth and differentiation can act as oncogenes and cause cellular transformation. These molecules represent attractive targets for cancer diagnosis or therapy and therefore are subject to intensive investigation. Aptamers are small, highly structured nucleic acid molecules, isolated from combinatorial libraries by a procedure termed SELEX. Aptamers bind to a target molecule by providing a limited number of specific contact points imbedded in a larger, defined three-dimensional structure. Recently, aptamers have been selected against whole living cells, opening a new path which presents three major advantages: (1) direct selection without prior purification of membrane-bound targets, (2) access to membrane proteins in their native conformation similar to the in vivo conditions and (3) identification of (new) targets related to a specific phenotype. The ability to raise aptamers against living cells opens some attractive possibilities for new therapeutic and delivery approaches. In this chapter, the most recent advances in the field will be reviewed together with detailed descriptions of the relevant experimental approaches.

Full Text

Duke Authors

Cited Authors

  • Cerchia, L; Giangrande, PH; McNamara, JO; de Franciscis, V

Published Date

  • 2009

Published In

Volume / Issue

  • 535 /

Start / End Page

  • 59 - 78

PubMed ID

  • 19377980

Pubmed Central ID

  • 19377980

International Standard Serial Number (ISSN)

  • 1064-3745

Digital Object Identifier (DOI)

  • 10.1007/978-1-59745-557-2_5

Language

  • eng

Conference Location

  • United States