The favorable IFNL3 genotype escapes mRNA decay mediated by AU-rich elements and hepatitis C virus-induced microRNAs.

Published

Journal Article

IFNL3, which encodes interferon-λ3 (IFN-λ3), has received considerable attention in the hepatitis C virus (HCV) field, as many independent genome-wide association studies have identified a strong association between polymorphisms near IFNL3 and clearance of HCV. However, the mechanism underlying this association has remained elusive. In this study, we report the identification of a functional polymorphism (rs4803217) in the 3' untranslated region (UTR) of IFNL3 mRNA that dictated transcript stability. We found that this polymorphism influenced AU-rich element (ARE)-mediated decay (AMD) of IFNL3 mRNA, as well as the binding of HCV-induced microRNAs during infection. Together these pathways mediated robust repression of the unfavorable IFNL3 polymorphism. Our data reveal a previously unknown mechanism by which HCV attenuates the antiviral response and indicate new potential therapeutic targets for HCV treatment.

Full Text

Duke Authors

Cited Authors

  • McFarland, AP; Horner, SM; Jarret, A; Joslyn, RC; Bindewald, E; Shapiro, BA; Delker, DA; Hagedorn, CH; Carrington, M; Gale, M; Savan, R

Published Date

  • January 2014

Published In

Volume / Issue

  • 15 / 1

Start / End Page

  • 72 - 79

PubMed ID

  • 24241692

Pubmed Central ID

  • 24241692

Electronic International Standard Serial Number (EISSN)

  • 1529-2916

Digital Object Identifier (DOI)

  • 10.1038/ni.2758

Language

  • eng

Conference Location

  • United States