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Identification of prohibitin and prohibiton as novel factors binding to the p53 induced gene 3 (PIG3) promoter (TGYCC)(15) motif.

Publication ,  Journal Article
Guan, X; Liu, Z; Wang, L; Johnson, DG; Wei, Q
Published in: Biochem Biophys Res Commun
January 24, 2014

The promoter of p53 induced gene 3 (PIG3) contains a variable number of tandem repeats (VNTRs) of pentanucleotides (TGYCC)n that is known as a p53 binding site. In this study, we investigated whether other potential molecules could bind to this PIG3 promoter (TGYCC)n motif. Ligand-chromatography combined with liquid chromatography-tandem mass spectrometry analyses indicated direct interactions of prohibitin and/or prohibiton with the (TGYCC)15 motif, which was confirmed by electrophoretic mobility shift assay and super-gel shift analysis with anti-prohibitin and anti-prohibiton antibodies. Using the chromatin immunopercipipation assay, we further demonstrated that prohibitin and prohibiton associated with the (TGYCC)15 motif in vivo regardless of the p53 status and apoptotic stress. We also found that prohibitin and prohibiton up-regulated PIG3 transcription independent of p53, although p53 obviously enhanced this process, and that the knock-down of prohibitin and prohibiton inhibited camptothecin-induced apoptosis. Taken together, our findings suggest that prohibitin and prohibiton contribute to PIG3-mediated apoptosis by binding to the PIG3 promoter (TGYCC)15 motif.

Duke Scholars

Published In

Biochem Biophys Res Commun

DOI

EISSN

1090-2104

Publication Date

January 24, 2014

Volume

443

Issue

4

Start / End Page

1239 / 1244

Location

United States

Related Subject Headings

  • Tumor Suppressor Protein p53
  • Repressor Proteins
  • RNA, Neoplasm
  • RNA, Messenger
  • Proto-Oncogene Proteins
  • Protein Binding
  • Promoter Regions, Genetic
  • Prohibitins
  • Microsatellite Repeats
  • Intracellular Signaling Peptides and Proteins
 

Citation

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Guan, X., Liu, Z., Wang, L., Johnson, D. G., & Wei, Q. (2014). Identification of prohibitin and prohibiton as novel factors binding to the p53 induced gene 3 (PIG3) promoter (TGYCC)(15) motif. Biochem Biophys Res Commun, 443(4), 1239–1244. https://doi.org/10.1016/j.bbrc.2013.12.124
Guan, Xiaoxiang, Zhensheng Liu, Luo Wang, David G. Johnson, and Qingyi Wei. “Identification of prohibitin and prohibiton as novel factors binding to the p53 induced gene 3 (PIG3) promoter (TGYCC)(15) motif.Biochem Biophys Res Commun 443, no. 4 (January 24, 2014): 1239–44. https://doi.org/10.1016/j.bbrc.2013.12.124.
Guan X, Liu Z, Wang L, Johnson DG, Wei Q. Identification of prohibitin and prohibiton as novel factors binding to the p53 induced gene 3 (PIG3) promoter (TGYCC)(15) motif. Biochem Biophys Res Commun. 2014 Jan 24;443(4):1239–44.
Guan, Xiaoxiang, et al. “Identification of prohibitin and prohibiton as novel factors binding to the p53 induced gene 3 (PIG3) promoter (TGYCC)(15) motif.Biochem Biophys Res Commun, vol. 443, no. 4, Jan. 2014, pp. 1239–44. Pubmed, doi:10.1016/j.bbrc.2013.12.124.
Guan X, Liu Z, Wang L, Johnson DG, Wei Q. Identification of prohibitin and prohibiton as novel factors binding to the p53 induced gene 3 (PIG3) promoter (TGYCC)(15) motif. Biochem Biophys Res Commun. 2014 Jan 24;443(4):1239–1244.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

EISSN

1090-2104

Publication Date

January 24, 2014

Volume

443

Issue

4

Start / End Page

1239 / 1244

Location

United States

Related Subject Headings

  • Tumor Suppressor Protein p53
  • Repressor Proteins
  • RNA, Neoplasm
  • RNA, Messenger
  • Proto-Oncogene Proteins
  • Protein Binding
  • Promoter Regions, Genetic
  • Prohibitins
  • Microsatellite Repeats
  • Intracellular Signaling Peptides and Proteins