Hypoxia inducible factor-alpha activation in lymphoma and relationship to the thioredoxin family.


Journal Article

Hypoxia inducible factors (HIFs) activate oncogenic pathways, while thioredoxins (Trx), including Trx1 and Trx reductases-1 and -2 (TrxR1 and TrxR2), promote HIF-alpha stabilization. In immunoblotting studies in lymphoma cell lines we found that Raji and SUDHL4 cells exhibited normoxic HIF-2alpha protein stabilization. Five cell lines showed increased TrxR1 expression, while only Namalwa, HF1 and SUDHL4 had Trx1 and TrxR2 activation. Tissue microarrays in diffuse large B-cell lymphoma (DLBCL) and follicular lymphoma (FL) identified different HIF expression among histological subgroups (e.g. 44% DLBCL vs. 11% of FL cases with moderate-to-high expression of HIF-1alpha and HIF-2alpha, P = 0.0017). These data demonstrate that HIF and the thioredoxin family are abnormally activated in lymphoma.

Full Text

Cited Authors

  • Evens, AM; Schumacker, PT; Helenowski, IB; Singh, ATK; Dokic, D; Keswani, A; Kordeluk, E; Raji, A; Winter, JN; Jovanovic, BD; Holmgren, A; Nelson, BP; Gordon, LI

Published Date

  • May 2008

Published In

Volume / Issue

  • 141 / 5

Start / End Page

  • 676 - 680

PubMed ID

  • 18422776

Pubmed Central ID

  • 18422776

Electronic International Standard Serial Number (EISSN)

  • 1365-2141

International Standard Serial Number (ISSN)

  • 0007-1048

Digital Object Identifier (DOI)

  • 10.1111/j.1365-2141.2008.07093.x


  • eng