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Pol II docking and pausing at growth and stress genes in C. elegans.

Publication ,  Journal Article
Maxwell, CS; Kruesi, WS; Core, LJ; Kurhanewicz, N; Waters, CT; Lewarch, CL; Antoshechkin, I; Lis, JT; Meyer, BJ; Baugh, LR
Published in: Cell reports
February 2014

Fluctuations in nutrient availability profoundly impact gene expression. Previous work revealed postrecruitment regulation of RNA polymerase II (Pol II) during starvation and recovery in Caenorhabditis elegans, suggesting that promoter-proximal pausing promotes rapid response to feeding. To test this hypothesis, we measured Pol II elongation genome wide by two complementary approaches and analyzed elongation in conjunction with Pol II binding and expression. We confirmed bona fide pausing during starvation and also discovered Pol II docking. Pausing occurs at active stress-response genes that become downregulated in response to feeding. In contrast, "docked" Pol II accumulates without initiating upstream of inactive growth genes that become rapidly upregulated upon feeding. Beyond differences in function and expression, these two sets of genes have different core promoter motifs, suggesting alternative transcriptional machinery. Our work suggests that growth and stress genes are both regulated postrecruitment during starvation but at initiation and elongation, respectively, coordinating gene expression with nutrient availability.

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Published In

Cell reports

DOI

EISSN

2211-1247

ISSN

2211-1247

Publication Date

February 2014

Volume

6

Issue

3

Start / End Page

455 / 466

Related Subject Headings

  • Transcriptional Elongation Factors
  • Transcription, Genetic
  • Transcription Initiation Site
  • Stress, Physiological
  • Sequence Analysis, RNA
  • RNA, Helminth
  • RNA Polymerase II
  • RNA Caps
  • Promoter Regions, Genetic
  • Mutation
 

Citation

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Maxwell, C. S., Kruesi, W. S., Core, L. J., Kurhanewicz, N., Waters, C. T., Lewarch, C. L., … Baugh, L. R. (2014). Pol II docking and pausing at growth and stress genes in C. elegans. Cell Reports, 6(3), 455–466. https://doi.org/10.1016/j.celrep.2014.01.008
Maxwell, Colin S., William S. Kruesi, Leighton J. Core, Nicole Kurhanewicz, Colin T. Waters, Caitlin L. Lewarch, Igor Antoshechkin, John T. Lis, Barbara J. Meyer, and L Ryan Baugh. “Pol II docking and pausing at growth and stress genes in C. elegans.Cell Reports 6, no. 3 (February 2014): 455–66. https://doi.org/10.1016/j.celrep.2014.01.008.
Maxwell CS, Kruesi WS, Core LJ, Kurhanewicz N, Waters CT, Lewarch CL, et al. Pol II docking and pausing at growth and stress genes in C. elegans. Cell reports. 2014 Feb;6(3):455–66.
Maxwell, Colin S., et al. “Pol II docking and pausing at growth and stress genes in C. elegans.Cell Reports, vol. 6, no. 3, Feb. 2014, pp. 455–66. Epmc, doi:10.1016/j.celrep.2014.01.008.
Maxwell CS, Kruesi WS, Core LJ, Kurhanewicz N, Waters CT, Lewarch CL, Antoshechkin I, Lis JT, Meyer BJ, Baugh LR. Pol II docking and pausing at growth and stress genes in C. elegans. Cell reports. 2014 Feb;6(3):455–466.
Journal cover image

Published In

Cell reports

DOI

EISSN

2211-1247

ISSN

2211-1247

Publication Date

February 2014

Volume

6

Issue

3

Start / End Page

455 / 466

Related Subject Headings

  • Transcriptional Elongation Factors
  • Transcription, Genetic
  • Transcription Initiation Site
  • Stress, Physiological
  • Sequence Analysis, RNA
  • RNA, Helminth
  • RNA Polymerase II
  • RNA Caps
  • Promoter Regions, Genetic
  • Mutation