In vitro electrophoresis and in vivo electrophysiology of peripheral nerve using DC field stimulation.

Journal Article (Journal Article)

BACKGROUND: Given the movement of molecules within tissue that occurs naturally by endogenous electric fields, we examined the possibility of using a low-voltage DC field to move charged substances in rodent peripheral nerve in vitro. NEW METHOD: Labeled sugar- and protein-based markers were applied to a rodent peroneal nerve and then a 5-10 V/cm field was used to move the molecules within the extra- and intraneural compartments. Physiological and anatomical nerve properties were also assessed using the same stimulation in vivo. RESULTS: We demonstrate in vitro that charged and labeled compounds are capable of moving in a DC field along a nerve, and that the same field applied in vivo changes the excitability of the nerve, but without damage. CONCLUSIONS: The results suggest that low-voltage electrophoresis could be used to move charged molecules, perhaps therapeutically, safely along peripheral nerves.

Full Text

Duke Authors

Cited Authors

  • Madison, RD; Robinson, GA; Krarup, C; Moldovan, M; Li, Q; Wilson, WA

Published Date

  • March 30, 2014

Published In

Volume / Issue

  • 225 /

Start / End Page

  • 90 - 96

PubMed ID

  • 24485870

Pubmed Central ID

  • PMC3971989

Electronic International Standard Serial Number (EISSN)

  • 1872-678X

Digital Object Identifier (DOI)

  • 10.1016/j.jneumeth.2014.01.018


  • eng

Conference Location

  • Netherlands